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Citations for Miltenyi Biotec :
51 - 100 of 653 citations for Granzyme B GZMB Mouse CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: Memory B cells and naïve B cells were isolated from RosetteSep pre-sorted total B cells using MACS cell sorting from Miltenyi Biotec. Memory B cells were obtained via positive selection using the Milteny Memory B cell isolation kit ...
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bioRxiv - Molecular Biology 2023Quote: ... CD43− B cells were enriched from the spleen by immunomagnetic depletion of non-B cells with CD43-MicroBeads (Miltenyi Biotec). The following monoclonal antibodies were used for flow-cytometric analysis of mouse lymphoid organs from 4-12 week-old mice ...
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bioRxiv - Cancer Biology 2023Quote: ... and B-CLL cells were further purified using the Pan B-cell Isolation Kit (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany). Versalyse buffer (Beckman-Coulter ...
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bioRxiv - Immunology 2022Quote: ... B cell kit (Miltenyi Biotec, 130-090-862) was followed according to the manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... naïve B cells were isolated from the spleens of mice using the B cell isolation kit (Miltenyi Biotec: 130-090-862) and 30×103 cultured on the irradiated 40LB cells with IL-4 in supplemented RPMI-1640 media (final ...
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bioRxiv - Immunology 2020Quote: ... of cytapheresis rings from healthy donors (EFS Bordeaux) and B cells were sorted with the B cell isolation kit II (Miltenyi Biotec). Sorted B cells were cultured for 4 days in RPMI 1640 with UltraGlutamine (Lonza ...
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bioRxiv - Immunology 2020Quote: Splenic B cells were isolated from 5 or 12 weeks old MRL/Lpr mice using B Cell Isolation Kit (Miltenyi Biotec) and stained with CSFE before co-culturing with purified TCRβ+CD138+ or TCRβ+CD138− cells in the presence of anti-CD3/CD28 antibodies (BD Biosciences) ...
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bioRxiv - Immunology 2021Quote: B cells were isolated from spleens of WT female mice and enriched with a B cell isolation kit (Miltenyi Biotec MACS). The purity of isolated B cells was 96±0.8% ...
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bioRxiv - Immunology 2021Quote: ... B cell isolation from cryopreserved PBMCs was carried out using Memory B cells Isolation Kit (MACS/Miltenyi Biotec, Bergisch Gladbach, Germany). Next ...
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bioRxiv - Immunology 2021Quote: Splenic B cells were isolated from 10 to 12 weeks old MRL/Lpr mice using B Cell Isolation Kit (Miltenyi Biotec). Isolated cells were washed three times with PBS ...
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bioRxiv - Immunology 2023Quote: PBMCs from three different donors were isolated from human buffy coat by Ficoll density gradient centrifugation and untouched B-cells were purified by negative magnetic enrichment using a B-cell isolation kit II (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: Resting B-cells were enriched from the spleen using the B-cell Isolation Kit (Miltenyi Biotec, Germany, Cat# 130-090-862). 1 mL 8 million/mL B-cells in HBSS buffer were sequentially incubated with 1 μM cholesterol strand (30 min ...
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bioRxiv - Immunology 2023Quote: ... B cell enrichment of bone marrow cells was performed using the Pan B Cell Isolation Kit (Miltenyi Biotec, #130-095-813). MultiScreen-IP Filter Plates (Sigma ...
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bioRxiv - Immunology 2023Quote: A cluster of differentiation 19 (CD19)-positive B lymphocytes (total B cells) were purified from buffy coats derived from healthy donors using magnetic microbeads (Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Microbiology 2021Quote: ... B-cells purified from donor PBMCs using the MACS Human B-Cells isolation kit (Miltenyi Biotec Miltenyi Biotec Cat# 130-091-151) were stained with a panel of antibodies ...
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bioRxiv - Microbiology 2021Quote: ... B-cells purified from donor PBMCs using the MACS Human B-Cells isolation kit (Miltenyi Biotec Miltenyi Biotec Cat# 130-091-151) were stained with a panel of antibodies ...
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bioRxiv - Microbiology 2021Quote: ... B lymphocyte isolation was conducted through negative selection by using the B cell Isolation Kit II (Miltenyi Biotec, Cat#130-091-151) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2022Quote: ... or Pan B Cell (Miltenyi Biotec, 130-104-433) isolation kits ...
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bioRxiv - Immunology 2023Quote: ... B cells were enriched with CD19 Microbeads (Miltenyi Biotec) or MojoSort Mouse Pan B cell Isolation Kit (BioLegend) ...
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bioRxiv - Immunology 2023Quote: ... with Whole Blood B Cell Isolation Kit (Miltenyi Biotec). Sample acquisition was approved by the Regional Ethics Committee of South-Eastern Norway (project 6544) ...
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bioRxiv - Immunology 2021Quote: ... were isolated by gradient centrifugation and then enriched for B cells by negative selection using a pan-B-cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec, 130-101-638).
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bioRxiv - Immunology 2021Quote: ... peripheral blood mononuclear cells were enriched for B cells by negative selection using a pan-B-cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were incubated in FACS buffer (1× PBS ...
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bioRxiv - Immunology 2021Quote: ... peripheral blood mononuclear cells were enriched for B cells by negative selection using a pan-B-cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were incubated in Flourescence-Activated Cell-sorting (FACS ...
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bioRxiv - Immunology 2022Quote: ... peripheral blood mononuclear cells were enriched for B cells by negative selection using a pan-B-cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were incubated in Flourescence-Activated Cell-sorting (FACS ...
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bioRxiv - Immunology 2022Quote: ... peripheral blood mononuclear cells were enriched for B cells by negative selection using a pan-B-cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were incubated in FACS buffer (1× PBS ...
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bioRxiv - Immunology 2022Quote: ... peripheral blood mononuclear cells were enriched for B cells by negative selection using a pan-B-cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were incubated in Flourescence-Activated Cell-sorting (FACS ...
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bioRxiv - Immunology 2021Quote: ... peripheral blood mononuclear cells were enriched for B cells by negative selection using a pan-B-cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were incubated in FACS buffer (1× PBS ...
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bioRxiv - Immunology 2021Quote: Murine naïve B cells were purified from spleen by magnetic negative selection by using Pan B Cell Isolation Kit (Miltenyi Biotec, 130-095-813) according to the manufacturer’s instructions and maintained in appropriate culture medium ...
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bioRxiv - Immunology 2020Quote: PBMCs were enriched for B cells by negative selection using a pan B cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were incubated in FACS buffer (1 X Phosphate-buffered Saline (PBS) ...
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bioRxiv - Immunology 2022Quote: ... were enriched for B cells by negative selection using a pan-B-cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were incubated in Flourescence-Activated Cell-sorting (FACS ...
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bioRxiv - Immunology 2022Quote: Splenic B cells from 24-week old MRL/lpr mice were isolated with a Pan B Cell Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany). B cells were cultured with RPMI-1640 medium (Lonza ...
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bioRxiv - Immunology 2023Quote: Single-cell suspensions of splenocytes from individual mice were prepared and B cell enrichment was performed using the Pan B Cell Isolation Kit (Miltenyi Biotec, #130-095-813). Plasmablast cell sorting was performed using a FACSAria (BD Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... B cells were additionally permeabilized with Inside Perm (Miltenyi Biotec) and stained with the unlabeled primary antibodies anti-NET ...
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bioRxiv - Bioengineering 2024Quote: CD43-negative B cells were collected using AutoMACS (Miltenyi Biotec, Inc. ...
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bioRxiv - Bioengineering 2024Quote: ... chambers were obtained from the Stanford Blood Center and primary human B cells were isolated by negative selection using the human B Cell Isolation Kit II (Miltenyi Biotec, cat: 130-091-151). Cells were cultured in Iscove’s modified Dulbecco’s medium (IMDM ...
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bioRxiv - Immunology 2020Quote: B cells were isolated from PBMCs using CD19-microbeads (Miltenyi Biotec) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2022Quote: ... Plasma B cells were isolated by CD138+ cell enrichment (Miltenyi Biotec CD138+ plasma cell isolation kit ...
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bioRxiv - Immunology 2022Quote: ... B cells were enriched from PBMCs with CD19 microbeads (Miltenyi Biotec). CD19+ cells were stained with anti-human AF700-CD20 (BD) ...
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bioRxiv - Cancer Biology 2020Quote: B cells were isolated from splenocyte suspensions using CD43 microbeads (Miltenyi Biotec), and plated at a density of 1×106/mL in B-cell media (DMEM high glucose with Glutamax (Gibco) ...
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bioRxiv - Genetics 2021Quote: ... and Human CD19+ B Cell Isolation kit (Miltenyi Biotec,130-050-301) respectively.
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bioRxiv - Immunology 2020Quote: ... Stained B cells were investigated with the MACSQuant Analyzer 10 (Miltenyi Biotec) and analyzed by using Flow-Jo or Flowlogic software.
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bioRxiv - Immunology 2021Quote: ... splenocytes were pre-enriched for B cells with B220 microbeads (Miltenyi Biotec) following manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... B cell enrichment kit was purchased from Miltenyi Biotec (Bergisch Gladbach, Germany). Recombinant mouse CD19 ...
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bioRxiv - Immunology 2021Quote: ... B cells were purified by negative selection (Miltenyi Biotec, Cat#130-091-151) from PBMCs and stimulated with R848 (1 ug/ml) ...
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bioRxiv - Cancer Biology 2020Quote: ... Naïve B cells were separated by positive selection using AutoMACS system (Miltenyi Biotec) after incubation with anti-IgD-FITC (BD Pharmingen ...
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Micro RNAs are minor constituents of extracellular vesicles and are hardly delivered to target cellsbioRxiv - Cell Biology 2020Quote: ... B cells were isolated using CD19 MicroBeads and MACS separation columns (Miltenyi Biotec), according to the manufacturer’s instruction.
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bioRxiv - Immunology 2022Quote: ... B cells were stimulated with 1 μg/ml CpG oligodeoxyribonucleotide 2006 (Miltenyi Biotec), 2.4 μg/ml F(ab’)2 Fragment Goat Anti-Human IgA + IgG + IgM (H+L ...
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bioRxiv - Immunology 2019Quote: ... Antibodies used were: CD19 PE (LT19) and CD138 APC (B-B4; Miltenyi Biotec); CD23 APC (M-L233) ...
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bioRxiv - Immunology 2022Quote: ... with the 37°C Multi-B program of a gentleMACS Dissociator (Miltenyi Biotec). The resulting single cell suspension was passed through a 70 µm cell strainer (Falcon) ...
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bioRxiv - Microbiology 2020Quote: ... we enriched B cells by incubating PBMCs with anti-CD20 MicroBeads (Miltenyi Biotec) followed by magnetic separation on a MACS LS column (Miltenyi Biotec ...