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Citations for Miltenyi Biotec :
401 - 450 of 1490 citations for Goat Anti Human IgM HRP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... anti-CD19 or anti-CD14 microbeads (Miltenyi Biotec). Enriched cells were then sorted as live CD45+ CD3+ CD4+ or CD45+ CD3+ CD8+ or CD45+ CD3neg CD19+ or CD45+ CD3neg CD14+ using FACS ARIA.
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bioRxiv - Immunology 2021Quote: Naive B cells were purified from PBMCs using the MACS Human B Cell isolation kit (Miltenyi Biotec) and incubated with 25nM of each SARS-CoV-2 probe (RBD-mFc-BV650 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Primary human T cells were purified from PBMCs by Pan T Cell Isolation Kit (Miltenyi Biotec, Germany). Purified T cells were stimulated with anti-CD3/CD28 Dynabeads (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... NK cells were isolated from PBMCs by negative depletion with human NK cell Isolation Kit (Miltenyi Biotec). Cells were cultured in RPMI 1640 medium supplemented with GlutaMAX (Gibco) ...
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bioRxiv - Cancer Biology 2022Quote: ... freshly isolated tumor samples were dissociated using the human tumor dissociation kit (Miltenyi Biotec; 130-095-929): surgical resection tissue was first minced into small pieces using a scalpel ...
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bioRxiv - Cancer Biology 2022Quote: ... 25 μL Enzyme A (all provided in the human tumor dissociation kit (Miltenyi Biotec; 130-095-929), and 4.7 mL DMEM (Gibco ...
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bioRxiv - Cancer Biology 2022Quote: ... The xenograft tumors were immediately digested using the human Tumor Dissociation Kit (130-095-929, Miltenyi Biotec) in combination with the GentleMACS Octo Dissociator (Miltenyi Biotec) ...
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bioRxiv - Developmental Biology 2021Quote: ... This enriched macrophage/monocyte solution was incubated with human and mouse CD11b microbeads (Miltenyi Biotec; 130093-634), and cells were further enriched for Cd11b-positive macrophages/monocytes using Miltenyi MACS Separation Columns (LS ...
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bioRxiv - Neuroscience 2022Quote: ... Human iPSCs (hiPSCs) were cultured in a chemically defined growth medium (StemMACS iPS-Brew XF (Miltenyi Biotec) or mTSeRTM1 (Stem Cell Technologies ...
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bioRxiv - Immunology 2021Quote: ... CD19+ B cells were purified from human PBMCs using the REAlease® CD19 Microbead Kit (Miltenyi Biotec) according to the manufacturer’s recommendations with purity greater than 98 %.
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bioRxiv - Genomics 2020Quote: Dissociation mixture was based on Miltenyi Human Tumor Dissociation Kit (Miltenyi Biotec, catalog no. 130-095-929). Before starting ...
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bioRxiv - Microbiology 2020Quote: ... 50 ng/ml recombinant human granulocyte macrophage colony–stimulating factor (rhGM-CSF) (130-093-865, Miltenyi Biotec), and 50 ng/ml rh interleukin (IL)-4 (130-093-921 ...
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bioRxiv - Immunology 2020Quote: ... iMS1 and iMono cells were isolated after 7 d of culture using human CD14 Microbeads (Miltenyi Biotec). Isolated CD14+ cells were validated using flow cytometry (CD14-FITC ...
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bioRxiv - Immunology 2021Quote: ... total PMBCs were enriched for B cells using a MACS human B cell isolation kit (Miltenyi Biotec). Purified B cells were enumerated ...
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bioRxiv - Molecular Biology 2022Quote: ... the human induced pluripotent stem cell (hiPSC) line HMGU12 was harvested using StemMACS Passaging Solution (Miltenyi Biotec) and resuspended in DMEM/F-12 medium supplemented with 20% KSR ...
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bioRxiv - Cell Biology 2022Quote: ... Human peripheral blood monocytes were isolated by the classical monocyte isolation kit (Miltenyi Biotec, #130-117-337), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: iMSC-like cells were acquired by stimulating ADSCs with recombinant human IL-1β (0.5ng/ml, Miltenyi Biotec) overnight ...
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bioRxiv - Biophysics 2023Quote: ... Human primary classical monocytes (CD14+CD16-) were further isolated using the Classical Monocyte Isolation Kit (Miltenyi Biotec) by magnetic-activated cell sorting (MACS) ...
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bioRxiv - Immunology 2023Quote: Human CD4+ T cells were isolated by employing a CD4+ T cells isolation kit (Miltenyi Biotec kit) following the provider’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Tumors harvested from mice were processed using the human tumor dissociation kit (Miltenyi Biotec, 130-095-929) with a GentleMacs Octo Dissociator with Heaters (Miltenyi Biotec ...
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bioRxiv - Systems Biology 2024Quote: ... and 25 μl of Enzyme A from the human Tumor Dissociation Kit (Miltenyi Biotec #130-095-929). C Tubes were placed in the gentleMACS Dissociator under the “Medium” Tumor type (gentleMACS Program 37C_h_TDK_2 ...
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bioRxiv - Immunology 2023Quote: ... CD14+ monocytes were isolated magnetically by positive selection using human CD14 MicroBeads (#130-050-201; Miltenyi Biotec) on LS columns (#130-042-401 ...
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bioRxiv - Immunology 2024Quote: ... Naïve CD4+ T cells were isolated using Human Naïve CD4+ T Cell Isolation Kit II (Miltenyi Biotec). CD14+ monocytes were isolated using Human CD14 Microbeads (Miltenyi Biotec) ...
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bioRxiv - Cancer Biology 2024Quote: Lungs harvested from mice were processed using the human tumor dissociation kit (Miltenyi Biotec, 130-095-929) with a GentleMacs Octo Dissociator with Heaters (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... Cells were restimulated with anti-CD2/anti-CD3/anti-CD28 (T cell activation beads, Miltenyi Biotec) to achieve a 1:1 bead/cell ratio in accordance with the manufacturer’s instructions for cell expansion ...
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bioRxiv - Cancer Biology 2021Quote: ... Freshly isolated PDX tumors were dissociated into single-cell suspensions using the Human Tumor Dissociation Kit (Miltenyi Biotec) and a gentleMACS™ Octo Dissociator (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... pDCs were further purified by magnetic sorting with human Diamond Plasmacytoid dendritic-cell isolation kit II (Miltenyi Biotec). Purity of cell preparation was assessed by flow cytometry and for all donors was more than 90%.
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bioRxiv - Immunology 2021Quote: ... The cell fraction was then depleted with human CD45 beads (100ul beads per 2×108 cells; Miltenyi Biotec) using autoMACS ...
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bioRxiv - Cancer Biology 2020Quote: ... cell suspensions were incubated for 15 minutes on ice with human FcR block (Miltenyi Biotec, Bergisch Gladbach, Germany). 1 x 106 cells were then distributed into control and experimental groups ...
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bioRxiv - Cancer Biology 2021Quote: ... Peripheral blood B cells (PBBC) were isolated using the human Total B cell isolation kit II (Miltenyi Biotec) as per manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... CD4 T cells were purified by negative selection using the human CD4 T Cell Isolation Kit (Miltenyi Biotec). T cells were activated with T cell activation/expansion CD2/3/28 beads at a ratio of 1:2 bead to cells (Miltenyi Biotec) ...
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bioRxiv - Cancer Biology 2022Quote: ... 12.5 μL Enzyme A (all provided in the human multi-tissues dissociation kit (Miltenyi Biotec; 130-110-201), and 2.35 mL DMEM (Gibco ...
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bioRxiv - Cancer Biology 2022Quote: ... Freshly isolated PDX tumors were dissociated into single-cell suspension using a Human Tumor Dissociation Kit (Miltenyi Biotec) and a gentleMACS™ Octo Dissociator (Miltenyi Biotec) ...
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bioRxiv - Neuroscience 2022Quote: Day 37 neurons were harvested using the Miltenyi MACS human mitochondria isolation kit (Miltenyi Biotec 130-094-532) with additional proteases added to the lysis buffer aprotinin (10 μg/ml) ...
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bioRxiv - Immunology 2022Quote: ... The RPMI medium was additionally supplemented with 200 ng/mL human Flt3 ligand (Miltenyi Biotec, 130-096-479) and 5 ng/mL murine GM-CSF (Peprotech ...
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bioRxiv - Bioengineering 2019Quote: ... Patient-derived GBM6 and GBM12 cells were stained with human-CD133 (PE conjugated 1:11 dilution; Miltenyi Biotec), then with human-CD68 (FITC conjugated 1:20 dilution ...
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bioRxiv - Immunology 2020Quote: Mouse monoclonal antibodies against human CD86 (clone FM95) and DC-SIGN (clone DCN47.5) were purchased from Miltenyi Biotec. Antibodies against human CD83 (clone HB15e ...
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bioRxiv - Molecular Biology 2021Quote: ... and the CD56+CD3− NK cells were isolated by using the human NK cell isolation kit (Miltenyi Biotec) following the manufacturer’s instructions.
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bioRxiv - Bioengineering 2021Quote: ... The CB cells were lineage depleted with the human lineage cell depletion kit (Miltenyi Biotec, 130-092-211), which contains the following biotin-conjugated monoclonal antibodies ...
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bioRxiv - Cancer Biology 2020Quote: ... Leland Chung) and PC3-mm (RRID: CVCL_4885) were blocked with human FcR blocking reagent (1:100; Miltenyi Biotec) and subsequently incubated with anti-CD117/c-kit-APC (1:50 ...
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bioRxiv - Immunology 2021Quote: ... Th1 cultures were activated with CD2/3/28 activation beads (Human T cell Activation/Expansion kit, Miltenyi Biotec) at a 2:1 ratio of cells to beads with anti-IL-4 (2μg/mL) ...
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bioRxiv - Microbiology 2021Quote: Purified rhesus and human CD4+ T cells were isolated from PBMCs using magnetic MACS CD4 MicroBeads (Miltenyi Biotec), as previously described (35) ...
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bioRxiv - Genomics 2021Quote: ... CD4+ T cells were then isolated using magnetic column separation (Miltenyi Biotec, CD4+ T cell isolation kit, human); 17.7 million CD4+ T cells ± 7.2 million were isolated from each subject ...
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bioRxiv - Cell Biology 2022Quote: ... and B cells and CD8+ T cells were isolated from these using the human CD19 Microbeads (Miltenyi Biotec) or the human CD8+ isolation kit (Miltenyi Biotec).
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bioRxiv - Cancer Biology 2022Quote: ... For tumor samples digestion was performed using tumor dissociation kit for human (130-095-929, MACS Miltenyi Biotec), mechanical dissociation using gentleMACSTM Dissociator running the gentleMACS program h_tumor_01 ...
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bioRxiv - Biophysics 2022Quote: ... and REA control (I)-APC human for the control of unspecific binding (Miltenyi Biotec, Ref. 130-104- 615). At least 10000 events were gated according to the FSC/SSC dot plot with the exclusion of dead cells.
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bioRxiv - Immunology 2023Quote: ... according to the manufacturer’s suggestions and monocytes purified using positive selection with paramagnetic CD14 (human) MicroBeads (Miltenyi Biotec). CD14+ monocytes were differentiated into macrophages using 100 ng/mL of recombinant human M-CSF (Immunotools ...
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Single-cell profiling and zebrafish avatars reveal LGALS1 as immunomodulating target in glioblastomabioRxiv - Cancer Biology 2023Quote: ... monocytes were isolated from the PBMCs using a monocyte isolation kit (Pan Monocyte Isolation Kit, human, Miltenyi Biotec). Next ...
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bioRxiv - Cancer Biology 2023Quote: ... and subsequently enriched for CD34+ cells using the human CD34 MicroBead kit (130-046-702 MACS Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Human peripheral blood mononuclear cells (PBMC) were purified from whole blood by density gradient centrifugation (FicollPaqueTM, Miltenyi Biotec). Whole blood was collected from healthy donors through an NIH Department of Transfusion Medicine (DTM)-approved protocol (Institutional Review Board of the National Institute of Allergy and Infectious Diseases [NIAID]) ...