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Citations for Miltenyi Biotec :
301 - 350 of 642 citations for Ephrin type A receptor 8 EphA8 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Human MSCs were cultured in StemMACS MSC Expansion Medium (Miltenyi Biotec, Bergisch Gladbach, Germany). Murine MSCs and LPR-MSCs were isolated and cultured as previously described (Albarenque et al. ...
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bioRxiv - Immunology 2020Quote: ... Cells were then incubated with CD11b MicroBeads (mouse/human; Miltenyi Biotec Inc., Auburn, CA) and passed through MACS® LS magnetic columns for positive selection of CD11b+ cells ...
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bioRxiv - Microbiology 2021Quote: ... After a 15 minute-incubation step with human Fc blocking reagent (MACS Miltenyi Biotec), cell surface markers were detected by a 30 minute-incubation at 4°C with 4 µg/mL Pacific Blue-conjugated mouse anti-CD14 (clone 63D3 ...
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bioRxiv - Immunology 2019Quote: ... filtered human AB serum then subjected to gentle mechanical dissociation using gentleMACS (Miltenyi Biotec) followed by short 20 min enzymatic dissociation at 37°C using a very low concentration of Liberase TL (0.042 mg/ml ...
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bioRxiv - Microbiology 2021Quote: ... PBMCs were incubated with anti-human CD14 antibodies coupled to magnetic beads (Miltenyi Biotec) and subjected to magnetic separation using LS columns (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2020Quote: ... and in presence of 33 U/ml human IL-2 (premium-grade, Miltenyi Biotec).
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bioRxiv - Immunology 2020Quote: Human and mouse lung tissues were digested using the Tumor Dissociation Kit (Miltenyi Biotec) for 30 minutes at 37°C and 1,500 rpm ...
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bioRxiv - Immunology 2020Quote: ... total PBMC were enriched in CD14+ monocytes using the human CD14 microbeads (Miltenyi Biotec) for positive magnetic selection according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... T cells were isolated using the human Pan T cell Isolation Kit (Miltenyi Biotec) and naive CD4+ T cell using the Naive CD4+ T Cell Isolation (Miltenyi Biotec) ...
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bioRxiv - Immunology 2020Quote: ... and 50 U/ml of human IL-2 IS (Miltenyi Biotec, #130-097-142) for primary T cells ...
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bioRxiv - Immunology 2022Quote: ... we used human IFNγ secretion assay detection kit (PE) (Miltenyi Biotec, #130-054-202) as per the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... PE CD158a/h (KIR2DL1/DS1) anti-Human antibody: 130-099-209 from Miltenyi Biotec. APC CD158b1/b2j (KIR2DL2/DL3/DS2 ...
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bioRxiv - Microbiology 2022Quote: ... Primary human T cells were purified by positive selection using CD3 MicroBeads (Miltenyi Biotec), as previously described (Goujon et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... or REA control human IgG1 APC-conjugated isotype antibody (Miltenyi Biotec, 130-120-709) for 1 h at room temperature in the dark in 0.75% saponin (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... human cells were incubated with anti-biotin microbeads (Miltenyi Biotec, Cat#130-090-485) in MACS buffer at 4°C for 15 min ...
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bioRxiv - Immunology 2022Quote: ... All setups were stained with 5μL of human FcR blocking reagent (Miltenyi Biotec, USA) to mitigate unspecific binding and LIVE/DEAD blue stain (Invitrogen ...
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bioRxiv - Genetics 2022Quote: ... Human bone marrow CD45+ cells were sorted by immunomagnetic selection with AutoMACS (Miltenyi Biotec) after immunostaining with the CD45 MicroBead Kit (Miltenyi Biotec) ...
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bioRxiv - Immunology 2023Quote: ... and CD38 (20 µl per 107 cells, from human CD38 MicroBead kit, Miltenyi Biotec) followed by depletion using magnetic anti-biotin beads (30 µl per 107 cells ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 100 ng/mL of recombinant human flt3 ligand (Miltenyi Biotec, Auburn, CA), stem cell factor (SCF ...
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bioRxiv - Microbiology 2022Quote: ... CD8 T cells were depleted using a kit from Miltenyi Biotec (CD8 MicroBeads, human) and cells cultured in RPMI-1640 (Life Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... Enzymes and reagents from the human tumor dissociation kit (Miltenyi Biotec: 130-095-929) were added to the tumor tissue along with 1.75 mL of DMEM ...
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bioRxiv - Immunology 2024Quote: ... 5% human AB serum (Biowest) and 40 IU/ml of IL-2 (Miltenyi Biotec). 24 hours later ...
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bioRxiv - Immunology 2024Quote: ... Human monocytes were negatively selected from PBMCs using the monocytes Isolation Kit (Miltenyi Biotec). Monocytes were then plated 0.15×106/well onto 96-well plate (for ELISA ...
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bioRxiv - Cancer Biology 2024Quote: ... Thawed and fresh samples were dissociated using the Tumor Dissociation Kit (human, Miltenyi Biotec) and tough dissociation protocol on gentleMACS Dissociator (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2020Quote: ... wild-type and JAK2V617F mutant marrow Lin-cKit+ HSPCs were isolated by flow sorting and magnetic bead isolation (Miltenyi Biotec, San Diego, CA). Total RNA was extracted using the RNeasy mini kit (Qiagen ...
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bioRxiv - Molecular Biology 2024Quote: ... Isolation of specific cell types was performed separately in male and female cultures An anti-CD11b microbeads antibody (Miltenyi Biotec, 130-049-601) and an anti-O4 microbeads antibody were used to isolate microglia and oligodendrocytes ...
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bioRxiv - Microbiology 2020Quote: ... T cells from the lungs of naïve or infected mice (day 8 pi) were enriched using the Pan T cell Isolation Kit II (Miltenyi Biotec) and co-cultured with infected BMDC at a ratio of 10:1 for 2-3 hours followed by the addition of Brefeldin A (5µg/ml ...
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bioRxiv - Immunology 2021Quote: Mouse naive T cells were purified from spleens and lymph nodes of 8-12 weeks old mice using the Naive CD4+ T Cell Isolation Kit according to the manufacturer’s instructions (Miltenyi Biotec). Cells were cultured in Iscove’s Modified Dulbecco’s Medium (IMDM ...
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bioRxiv - Immunology 2021Quote: ... 8-12-week-old mice were intraperitoneally injected with 10 million CD4+ T cells (Miltenyi Biotec, Cat#130-104-454) from Bm12 mice purified by magnetic cell sorting and mice were sacrificed for analysis at indicated time points ...
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ADAR2 deaminase activity promotes Th17 effector function and protects against intestine inflammationbioRxiv - Immunology 2022Quote: Mouse naive T cells were purified from spleens and lymph nodes of 8-12 weeks old mice using the Naive CD4+ T Cell Isolation Kit according to the manufacturer’s instructions (Miltenyi Biotec). Cells were cultured in Iscove’s Modified Dulbecco’s Medium (IMDM ...
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bioRxiv - Immunology 2022Quote: Mouse naive T cells were purified from spleens and lymph nodes of 8-12 weeks old mice using the Naive CD4+ T Cell Isolation Kit according to the manufacturer’s instructions (Miltenyi Biotec). Cells were cultured in Iscove’s Modified Dulbecco’s Medium (IMDM ...
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bioRxiv - Immunology 2021Quote: Naive CD4+ T cells from spleens of wild-type C57BL/6J and P2rx7-/- mice were sorted with MACS columns (Miltenyi Biotec, Bergisch Gladbach, Germany). For cytokine-skewed Th17 differentiation ...
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bioRxiv - Immunology 2021Quote: αβT cells transduced with γδTCR-Cl5 either alone or together with NKG2D wild type or the different NKG2D chimeras were incubated with a biotin-labeled anti-αβTCR antibody (clone BW242/412; Miltenyi Biotec, Bergisch Gladbach, Germany) and subsequently incubated with an anti-biotin antibody coupled to magnetic beads (anti-biotin MicroBeads ...
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bioRxiv - Genomics 2020Quote: ... Structures were enzymatically dissociated into single cells using the Tumor Dissociation Kit (human) (Miltenyi Biotec) with a gentleMACS™ Octo Dissociator (with heaters ...
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bioRxiv - Immunology 2019Quote: ... CD4 T cells were isolated from healthy human donor PBMCs by negative selection (Miltenyi Biotec), and activated with plate-bound anti-CD3 (clone OKT3 ...
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bioRxiv - Cell Biology 2021Quote: Human neutrophils were isolated on a MACS LS column system (Miltenyi Biotec #130-042-401), enriched with magnetic anti-CD15 antibodies (Miltenyi Biotec #130-046-601 ...
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bioRxiv - Cancer Biology 2020Quote: ... and anti-human CD38 (IB6, PE, Miltenyi Biotec, Cat No 130-113-989, 1:50) antibodies ...
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bioRxiv - Immunology 2022Quote: ... resting NK cells were obtained using the human NK cell isolation kit (MACS Miltenyi Biotec), following manufacture’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... and CD4+ T cells were isolated using human CD4+T cell isolation kit (Miltenyi Biotec). Purified CD4+T cells were washed and stained with 3 µM CFSE (Invitrogen) ...
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bioRxiv - Bioengineering 2022Quote: ... Human monocytes were isolated from PBMC using a magnetic-based monocyte isolation kit (Miltenyi Biotec) and differentiated into macrophages with GMCSF (50 ng/mL ...
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bioRxiv - Systems Biology 2020Quote: ... Monocytes were positively selected by a magnetic field using Human CD14 MicroBeads (MACS Miltenyi Biotec) and LS Columns (Miltenybiotec) ...
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bioRxiv - Immunology 2019Quote: ... human CAR T cells were sorted for CD34+ (CD34 MicroBead Kit UltraPure, MACS, Miltenyi Biotec) CAR T cells and expanded for up to 15 days in the presence of human IL-2 ...
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bioRxiv - Immunology 2020Quote: ... plus 10ng/ml recombinant human IL-2 and IL-4 (Miltenyi Biotec, Auburn, CA, USA). Cells were maintained in a humidified incubator at 37°C with 5% CO2 ...
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bioRxiv - Synthetic Biology 2020Quote: ... T cells were stimulated and expanded using TransAct Human T cell activation reagent (Miltenyi Biotec). For transduction ...
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bioRxiv - Immunology 2019Quote: ... density centrifugation followed by magnetic selection with CD14 Human MicroBeads (130-050-201, Miltenyi Biotec). Monocytes were incubated for 7 days with RPMI-1640 (Gibco ...
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bioRxiv - Cancer Biology 2021Quote: ... HDMECs were then isolated using a human CD31 MicroBead kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... monocytes were purified from human PBMC by using CD14 microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instruction ...
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bioRxiv - Genomics 2020Quote: ... anti-human EPCAM antibody conjugated to PE (1:50 dilution, Miltenyi Biotec #130-091-253), and either Calcein-AM (1:200 dilution ...
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bioRxiv - Immunology 2022Quote: ... CD8 or CD4 T cells were purified from human PBMCs using isolation kits (Miltenyi Biotec) following manufacturer’s instruction ...
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bioRxiv - Immunology 2022Quote: ... T cells were purified using the human Naïve Pan T Cell Isolation Kit (Miltenyi Biotec) and NK cells using the human NK Cell Isolation Kit (Miltenyi Biotec) ...