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Citations for Miltenyi Biotec :
501 - 550 of 2751 citations for Dengue Virus Serotype 4 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... while bone marrow (BM) cells were depleted of T cells by using CD90.2 magnetic beads (Miltenyi Biotec). C57BL/6 (syngeneic) ...
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bioRxiv - Immunology 2019Quote: ... Total B-cells were isolated by negative selection with the Memory B-cell Isolation Kit (Miltenyi Biotec). Memory-enriched B cells fractions were isolated by negative selection following incubation of total ...
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bioRxiv - Biochemistry 2021Quote: ... Splenic T cells were purified using T cell isolation kits and AutoMacs magnetic separation system (Miltenyi Biotec) and approximately 90% of the purified cells displayed a naïve phenotype as previously described19 ...
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bioRxiv - Immunology 2019Quote: ... CD4+ CD8+ T cell subsets were isolated via magnetic separation using Pan T cell kit (Miltenyi Biotec). Effector-T-cells (CD25− ...
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bioRxiv - Immunology 2020Quote: ... TCRβ+CD4+CD138− cells were isolated from TCRβ+CD138− cells using the CD4 (L3T4) MicroBeads (Miltenyi Biotec), and unbound cells were identified as TCRβ+CD8+CD138− cells.
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bioRxiv - Cell Biology 2021Quote: ... dissociated cells were incubated with CD90 microbeads (Miltenyi Biotec, 130-096-253; 1 μl per 107 cells) to enrich CD90+ NPCs.
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bioRxiv - Biochemistry 2021Quote: ... The CD73 positive cells were collected as photoreceptor cells using an autoMACS Pro Separator (Miltenyi Biotec, USA.).
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bioRxiv - Immunology 2021Quote: ... Naïve CD4 T cells were isolated using the Naïve CD4 T Cell Isolation Kit II (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Murine CD4+ T cells were purified using the MACS CD4+ T cell negative selection kit (Miltenyi Biotec) and naïve CD4+ T cells from WT ...
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bioRxiv - Immunology 2020Quote: ... γδ T cells were isolated from this suspension using the γδTCR+ T cell Isolation Kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... total PMBCs were enriched for B cells using a MACS human B cell isolation kit (Miltenyi Biotec). Purified B cells were enumerated ...
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bioRxiv - Immunology 2021Quote: Single-cell suspensions from mouse splenocytes were prepared using a cell dissociator (GentleMACS Octo Dissociator, Miltenyi Biotec) based on a previously optimized protocol28 ...
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bioRxiv - Cell Biology 2022Quote: Cells were purified by FACS 72 h post-isolation on a MACSQuant Tyto Cell Sorter (Miltenyi Biotec) following antibody staining as described above ...
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bioRxiv - Microbiology 2022Quote: ... and CD14+ cells were isolated using the magnetic MACS Cell Separation system with CD14 microbeads (Miltenyi Biotec). Cells were incubated in RPMI 1640 medium (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: ... murine CD4+ T cells were purified using the MACS CD4+ T cell negative selection kit (Miltenyi Biotec) and naïve CD4+ T cells from WT ...
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bioRxiv - Immunology 2023Quote: ... CD4+ T cells were then enriched using a nonhuman primate CD4+ T cell isolation kit (Miltenyi Biotec). 1×104 ...
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bioRxiv - Immunology 2023Quote: Human CD4+ T cells were isolated by employing a CD4+ T cells isolation kit (Miltenyi Biotec kit) following the provider’s instructions ...
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bioRxiv - Immunology 2022Quote: ... OT-I T cells were purified from spleens with the CD8a+ T Cell Isolation Kit (Miltenyi Biotec), activated and cultured as previously described (11).
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bioRxiv - Cancer Biology 2023Quote: ... B cells were immunomagnetically separated using the MACS mouse B cells isolation kit (Miltenyi Biotec, Auburn, CA) and L.S ...
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bioRxiv - Developmental Biology 2023Quote: ... red blood cells were lysed with a Red Blood Cell Lysis kit (Miltenyi Biotec, 130-094-183), per manufacturer instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... CD8+ T cells were sorted from PBMCs using magnetic bead-based cell sorting CD8 MicroBeads (Miltenyi Biotec), the process was performed according to the manufacture’s instruction ...
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bioRxiv - Immunology 2023Quote: ... CD4+ T cells were enriched via Magnetic-activated Cell Sorting (MACS) using CD4 (L3T4) microbeads (Miltenyi Biotec). In some instances ...
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bioRxiv - Immunology 2024Quote: ... while donor-matched natural killer (NK) cells were isolated using an NK cell isolation kit (Miltenyi Biotec) and cultured overnight in hR10 supplemented with IL-15 at 10 ng/mL (PeproTech) ...
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bioRxiv - Genomics 2023Quote: ... the dissociated single-cell suspensions were thawed and filtered using a 70 μm cell strainer (Miltenyi Biotec) to remove debris ...
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bioRxiv - Genomics 2023Quote: ... Lineage cells were depleted using Mouse Lineage Cell Depletion Kit (catalog no. 130-110-470, Miltenyi BIotec) and pass through LD depletion columns (catalog no ...
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bioRxiv - Genomics 2023Quote: ... Live cell enrichment was performed using MACS Dead Cell Removal Kit (Miltenyi Biotec, cat#: 130-090-101) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells were enriched from PBMCs using the untouched CD8+ T Cell Isolation Kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... The cell suspension was enriched in CD11b+ cells through positive selection using CD11b microbeads (MACS, Miltenyi Biotec). The purity of CD11b+ cells was ∼90% as determined by FACS ...
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bioRxiv - Pathology 2024Quote: ... Dead cells were removed using a dead cell removal kit (Catalog No. 130-090-101, Miltenyi Biotec). Cardiomyocytes were purified using a PSC-Derived Cardiomyocyte Isolation Kit (130-110-188 ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells were negatively selected from the spleenocytes using CD4+ T cell isolation kit (Miltenyi Biotec) and cultured in RPMI 1640 media supplemented with 25 mM HEPES ...
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bioRxiv - Immunology 2024Quote: ... Naïve CD4+ T cells were isolated using Human Naïve CD4+ T Cell Isolation Kit II (Miltenyi Biotec). CD14+ monocytes were isolated using Human CD14 Microbeads (Miltenyi Biotec) ...
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bioRxiv - Bioengineering 2024Quote: ... B cells were then isolated with Pan B Cell Isolation Kit II (Miltenyi Biotec, 130-104-443) and LS columns (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... untouched Pan T cells were enriched by negative selection using T cell isolation kit II (Miltenyi biotec). All donor tissue information is included in Table S1.
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bioRxiv - Immunology 2024Quote: ... Human T cells were enriched from PBMCs using the EasySep Negative Human T Cell kit (Miltenyi Biotec) according to manufacturer instructions and labeled with 5 M Tag-it Violet (Biolegend ...
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bioRxiv - Neuroscience 2021Quote: ... we prepared single-cell suspensions from spleens of 4- and 12-month-old APPtgp38fl/flLysM-Cre+/- and APPtgp38fl/flLysM-Cre-/- littermate mice with the Spleen Dissociation Kit (Miltenyi Biotec B.V. & Co. KG). After blocking with 25 μg/ml rat anti-mouse CD16/CD32 antibody (clone 2.4G2 ...
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bioRxiv - Cancer Biology 2019Quote: ... tumor samples were cut into pieces of 2-4 mm and suspended using the tumor dissociation protocol with the GentleMacs® dissociator (Miltenyi Biotec GmbH, Germany). The cell suspension was applied to a cell strainer (70 μm ...
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bioRxiv - Plant Biology 2022Quote: ... Extracted protein samples were centrifuged at 20 000g for 2 min at 4°C and the supernatant was collected for immunoprecipitation using the µMACS GFP Isolation Kit (Miltenyi Biotec, MediRay, New Zealand). Total and immunoprecipitated proteins were resolved on a 4–12% SDS-PAGE gel ...
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bioRxiv - Cancer Biology 2023Quote: ... 2-4 mm pieces of tumors from huPDAC mice were treated with an enzyme mix from a tumor dissociation kit (Miltenyi Biotec, Bergisch Gladbach, Germany). The tissues were dissociated using a gentle MACS Dissociator (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2023Quote: Tumors from orthotopic PDAC mice were minced into 2-4 mm pieces and added with an enzyme mix from a tumor dissociation kit (Miltenyi Biotec, Bergisch Gladbach, Germany) in Roswell Park Memorial Institute medium 1640 (Cytiva ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were immunoprecipitated by adding 30ul of α-c-Myc magnetic beads (μMACS Anti-c-myc Miltenyi Biotec, cat# 130-091-284) and incubated for 2h at 4°C with rotation ...
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bioRxiv - Immunology 2021Quote: ... The cells were incubated for 18 h at 37 °C at 1.5×106 cells per well with 2 μg/ml of SARS-CoV-2 Spike protein peptide pool (PepTivator® SARS-CoV-2 Prot_S, Miltenyi Biotec, Germany). Cells were stained with anti-CD8α (APC) ...
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bioRxiv - Immunology 2021Quote: ... Cells were cultured for 18 h at 5×105 cells per well with 2 μg/ml of a peptide pool consisting mainly of 15-mers (overlapping by 11 amino acids) covering the immunodominant sequence domains of the SARS-CoV-2 Spike protein (PepTivator® SARS-CoV-2 Prot_S; Miltenyi Biotec, Germany). The number of spots was determined using an automatic ELISPOT reader and image analysis software (CTL-ImmunoSpot® S6 Micro Analyzer ...
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bioRxiv - Microbiology 2022Quote: ... and stimulated with either SARS-CoV-2 S-trimer (10-100 µg/mL) or SARS-CoV-2 PepTivator® Peptide S and NP protein Pools (10 µg/mL each, Miltenyi Biotec) for 72 h at 37°C ...
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bioRxiv - Immunology 2021Quote: ... bone marrow of RBD-his tag protein immunized mice were isolated and purified with mouse CD138 MicroBeads (Miltenyi Biotec, 130-098-257) following standard protocol provided by the manufacturer ...
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bioRxiv - Cell Biology 2020Quote: ... Stro-1 positive cells were collected by magnetic-activated cell sorting (MACS) according to manufacturer’s instructions (Miltenyi Biotec).
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bioRxiv - Immunology 2021Quote: ... The cell fraction was then depleted with human CD45 beads (100ul beads per 2×108 cells; Miltenyi Biotec) using autoMACS ...
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bioRxiv - Developmental Biology 2022Quote: ... Muscle stem cells (MuSCs) were isolated from mouse TA muscle using a satellite cell isolation kit (Miltenyi Biotec) following the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... or from enriched mouse lung CD4+ T cells (CD4 T cell isolation kit, Miltenyi Biotec, Auburn, CA, USA), using RNeasy Micro Kit (Qiagen ...
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bioRxiv - Cancer Biology 2021Quote: ... Peripheral blood B cells (PBBC) were isolated using the human Total B cell isolation kit II (Miltenyi Biotec) as per manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: CD8+/-immune cell fractions were isolated by magnetic-activated cell sorting (130-045-201, CD8 MicroBeads, Miltenyi Biotec). Immune cells were allowed to rest for 24h ...