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Citations for Miltenyi Biotec :
451 - 500 of 2724 citations for Dengue Virus Serotype 3 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... 1 ml of protein extract was mixed with 50 µl of magnetic beads from µMACS™ GFP Isolation Kit (Miltenyi Biotec) and the mixture was incubated on ice while mild shaking ...
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bioRxiv - Cell Biology 2022Quote: ... aliquots of isolated mitochondria (1 µg total protein each) first had 100 ng mRNA spike-in control added (StemMACS eGFP mRNA #130-101-114, Miltenyi Biotec), and were then incubated with or without triton-x (1% v/v ...
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bioRxiv - Cell Biology 2019Quote: ... The MicroBeads/cell extracts mixture was then applied to the μColumn followed by gentle wash with the lysis buffer used above for protein extraction (Miltenyi Biotec). Pre-heated (95°C ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were detected with anti-TuMV coat protein antibody at a concentration of 1:1500 and Goat anti-Rabbit IgG (H+L)-HRP conjugate (Miltenyi Biotec) at a concentration of 1:10,000 ...
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bioRxiv - Cancer Biology 2022Quote: ... we separate the epidermal tissue from the dermis and then dissociated to single-cell suspensions by combining mechanical dissociation with enzymatic degradation of the extracellular adhesion proteins using Epidermis Dissociation Kit for Human (130-103-464, MACS Miltenyi Biotec). The dissociated cell suspension was strained with a 40μm filter (BD Falcon) ...
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bioRxiv - Immunology 2023Quote: ... Duplicate cultures were stimulated with or without a combined preparation of Peptivator Peptide Pools of the complete Spike protein and predicted immunodominant sequences (Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Immunology 2021Quote: Naive B cells were purified from PBMCs using the MACS Human B Cell isolation kit (Miltenyi Biotec) and incubated with 25nM of each SARS-CoV-2 probe (RBD-mFc-BV650 ...
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bioRxiv - Systems Biology 2019Quote: ... 100 μl per 10 million cells of MACS dead cell removal beads (Miltenyi Biotec, 130-090-101) were added and incubated according to the product’s accompanying protocols ...
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bioRxiv - Biophysics 2019Quote: ... T cells were isolated from PBMCs with Pan T cell isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany), then were stimulated for 48⍰h with anti-CD3/anti-CD28 Dynabeads (Gibco by Thermo Fischer Scientific ...
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bioRxiv - Immunology 2019Quote: ... CTV-labeled T cells were then isolated using a Pan naïve T cell isolation kit (Miltenyi Biotec) according to manufacturer’s instructions and isolation purity (≥95% ...
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bioRxiv - Synthetic Biology 2021Quote: ... Primary human T cells were purified from PBMCs by Pan T Cell Isolation Kit (Miltenyi Biotec, Germany). Purified T cells were stimulated with anti-CD3/CD28 Dynabeads (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... NK cells were isolated from PBMCs by negative depletion with human NK cell Isolation Kit (Miltenyi Biotec). Cells were cultured in RPMI 1640 medium supplemented with GlutaMAX (Gibco) ...
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bioRxiv - Cancer Biology 2022Quote: ... RBC lysed and dead cells were separated (using dead cell removal kit, Miltenyi Biotec, 130-090-101). The live cells were then resuspended in a sort buffer to inject 1 million cells per B6 MMTV-PyMT mouse retro-orbitally.
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bioRxiv - Systems Biology 2022Quote: ... and MM cells were enriched by CD138-positive selection with magnetic activated cell separation microbeads (Miltenyi Biotec). MM cells were cultured in RPMI containing 100 U/ml penicillin and 100 ug/ml streptomycin ...
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bioRxiv - Immunology 2022Quote: ... and further enriched for memory cells by depletion of naïve T cells using CD45RA beads (Miltenyi Biotec), prior to lectin staining and sorting as described further below ...
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bioRxiv - Immunology 2020Quote: ... while bone marrow (BM) cells were depleted of T cells by using CD90.2 magnetic beads (Miltenyi Biotec). C57BL/6 (syngeneic) ...
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bioRxiv - Immunology 2019Quote: ... Total B-cells were isolated by negative selection with the Memory B-cell Isolation Kit (Miltenyi Biotec). Memory-enriched B cells fractions were isolated by negative selection following incubation of total ...
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bioRxiv - Biochemistry 2021Quote: ... Splenic T cells were purified using T cell isolation kits and AutoMacs magnetic separation system (Miltenyi Biotec) and approximately 90% of the purified cells displayed a naïve phenotype as previously described19 ...
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bioRxiv - Immunology 2019Quote: ... CD4+ CD8+ T cell subsets were isolated via magnetic separation using Pan T cell kit (Miltenyi Biotec). Effector-T-cells (CD25− ...
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bioRxiv - Immunology 2020Quote: ... TCRβ+CD4+CD138− cells were isolated from TCRβ+CD138− cells using the CD4 (L3T4) MicroBeads (Miltenyi Biotec), and unbound cells were identified as TCRβ+CD8+CD138− cells.
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bioRxiv - Cell Biology 2021Quote: ... dissociated cells were incubated with CD90 microbeads (Miltenyi Biotec, 130-096-253; 1 μl per 107 cells) to enrich CD90+ NPCs.
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bioRxiv - Biochemistry 2021Quote: ... The CD73 positive cells were collected as photoreceptor cells using an autoMACS Pro Separator (Miltenyi Biotec, USA.).
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bioRxiv - Immunology 2021Quote: ... Naïve CD4 T cells were isolated using the Naïve CD4 T Cell Isolation Kit II (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Murine CD4+ T cells were purified using the MACS CD4+ T cell negative selection kit (Miltenyi Biotec) and naïve CD4+ T cells from WT ...
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bioRxiv - Immunology 2020Quote: ... γδ T cells were isolated from this suspension using the γδTCR+ T cell Isolation Kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... total PMBCs were enriched for B cells using a MACS human B cell isolation kit (Miltenyi Biotec). Purified B cells were enumerated ...
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bioRxiv - Immunology 2021Quote: Single-cell suspensions from mouse splenocytes were prepared using a cell dissociator (GentleMACS Octo Dissociator, Miltenyi Biotec) based on a previously optimized protocol28 ...
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bioRxiv - Cell Biology 2022Quote: Cells were purified by FACS 72 h post-isolation on a MACSQuant Tyto Cell Sorter (Miltenyi Biotec) following antibody staining as described above ...
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bioRxiv - Microbiology 2022Quote: ... and CD14+ cells were isolated using the magnetic MACS Cell Separation system with CD14 microbeads (Miltenyi Biotec). Cells were incubated in RPMI 1640 medium (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: ... murine CD4+ T cells were purified using the MACS CD4+ T cell negative selection kit (Miltenyi Biotec) and naïve CD4+ T cells from WT ...
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bioRxiv - Immunology 2023Quote: ... CD4+ T cells were then enriched using a nonhuman primate CD4+ T cell isolation kit (Miltenyi Biotec). 1×104 ...
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bioRxiv - Immunology 2023Quote: Human CD4+ T cells were isolated by employing a CD4+ T cells isolation kit (Miltenyi Biotec kit) following the provider’s instructions ...
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bioRxiv - Immunology 2022Quote: ... OT-I T cells were purified from spleens with the CD8a+ T Cell Isolation Kit (Miltenyi Biotec), activated and cultured as previously described (11).
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bioRxiv - Cancer Biology 2023Quote: ... B cells were immunomagnetically separated using the MACS mouse B cells isolation kit (Miltenyi Biotec, Auburn, CA) and L.S ...
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bioRxiv - Developmental Biology 2023Quote: ... red blood cells were lysed with a Red Blood Cell Lysis kit (Miltenyi Biotec, 130-094-183), per manufacturer instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... CD8+ T cells were sorted from PBMCs using magnetic bead-based cell sorting CD8 MicroBeads (Miltenyi Biotec), the process was performed according to the manufacture’s instruction ...
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bioRxiv - Immunology 2023Quote: ... CD4+ T cells were enriched via Magnetic-activated Cell Sorting (MACS) using CD4 (L3T4) microbeads (Miltenyi Biotec). In some instances ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells were enriched from PBMCs using the untouched CD8+ T Cell Isolation Kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... The cell suspension was enriched in CD11b+ cells through positive selection using CD11b microbeads (MACS, Miltenyi Biotec). The purity of CD11b+ cells was ∼90% as determined by FACS ...
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bioRxiv - Genomics 2023Quote: ... Live cell enrichment was performed using MACS Dead Cell Removal Kit (Miltenyi Biotec, cat#: 130-090-101) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Lineage cells were depleted using Mouse Lineage Cell Depletion Kit (catalog no. 130-110-470, Miltenyi BIotec) and pass through LD depletion columns (catalog no ...
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bioRxiv - Genomics 2023Quote: ... the dissociated single-cell suspensions were thawed and filtered using a 70 μm cell strainer (Miltenyi Biotec) to remove debris ...
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bioRxiv - Immunology 2024Quote: ... untouched Pan T cells were enriched by negative selection using T cell isolation kit II (Miltenyi biotec). All donor tissue information is included in Table S1.
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bioRxiv - Immunology 2024Quote: ... Human T cells were enriched from PBMCs using the EasySep Negative Human T Cell kit (Miltenyi Biotec) according to manufacturer instructions and labeled with 5 M Tag-it Violet (Biolegend ...
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bioRxiv - Pathology 2024Quote: ... Dead cells were removed using a dead cell removal kit (Catalog No. 130-090-101, Miltenyi Biotec). Cardiomyocytes were purified using a PSC-Derived Cardiomyocyte Isolation Kit (130-110-188 ...
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bioRxiv - Immunology 2024Quote: ... while donor-matched natural killer (NK) cells were isolated using an NK cell isolation kit (Miltenyi Biotec) and cultured overnight in hR10 supplemented with IL-15 at 10 ng/mL (PeproTech) ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells were negatively selected from the spleenocytes using CD4+ T cell isolation kit (Miltenyi Biotec) and cultured in RPMI 1640 media supplemented with 25 mM HEPES ...
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bioRxiv - Immunology 2024Quote: ... Naïve CD4+ T cells were isolated using Human Naïve CD4+ T Cell Isolation Kit II (Miltenyi Biotec). CD14+ monocytes were isolated using Human CD14 Microbeads (Miltenyi Biotec) ...
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bioRxiv - Bioengineering 2024Quote: ... B cells were then isolated with Pan B Cell Isolation Kit II (Miltenyi Biotec, 130-104-443) and LS columns (Miltenyi Biotec ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were immunoprecipitated by adding 30ul of α-c-Myc magnetic beads (μMACS Anti-c-myc Miltenyi Biotec, cat# 130-091-284) and incubated for 2h at 4°C with rotation ...