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Citations for Miltenyi Biotec :
551 - 600 of 2875 citations for Dengue Virus Serotype 2 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... Dead cells are removed using Dead Cell Removal Kit (Miltenyi Biotec, cat. no. 130-090-101). 1×106 PBMCs were crosslinked in 1 ml freshly prepared glyoxal fixation buffer (3% glyoxal ...
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bioRxiv - Immunology 2023Quote: CD8+ T-cells were isolated from HLA.A2- donors using magnetic-activated cell sorting (MACS, Miltenyi Biotec). Anti-CD3/CD28/CD2 Immunocult (Stemcell Technologies ...
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bioRxiv - Pathology 2023Quote: Endothelial cells were isolated from single cell suspension using LS Columns (#130-042-401, Miltenyi Biotec) and MACs magnetic beads with negative selection of CD45 (#130-052-301 ...
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bioRxiv - Cancer Biology 2023Quote: ... dead cell removal was performed using dead cell removal kit (Miltenyi Biotec, Catalog #: 130-090-101) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... The cells were then passed through a 30 μM cell strainer (Miltenyi Biotec 130-098-458) and assessed for viability and doublets before being pelleted and washed 3x in buffer (PBS + 0.04% BSA + Y-27632 (10 μM)) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Dead and dying cells were filtered using the Dead Cell Removal Kit (Miltenyi Biotec, Cologne, Germany) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... and dead cell removal using the Dead Cell Removal Kit (130-090-101, MACS Miltenyi Biotec) to confirm cell viability >85% with trypan blue staining (Invitrogen) ...
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bioRxiv - Developmental Biology 2023Quote: ... Dead cells were then removed with a Dead Cell Removal kit (Miltenyi Biotec 130-090-101) and MACS MS columns (Miltenyi Biotec) ...
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bioRxiv - Immunology 2023Quote: ... naïve CD4+ T cells were purified using the naïve CD4+ T cell isolation Kit (Miltenyi Biotec). Naïve T cells were cultured at a concentration of 1.5–2×106/ml in complete RPMI 1640 medium (supplemented with 10% heat-inactivated FBS ...
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bioRxiv - Immunology 2023Quote: ... to exclude CD3-positive T cells in the cell suspension using anti-biotin microbeads (Miltenyi Biotec) and LS columns (Miltenyi Biotec) ...
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bioRxiv - Cancer Biology 2024Quote: ... Mouse cell contaminations were removed using the Mouse Cell Depletion Kit (Miltenyi Biotec, 130-104-694). Tertiary tNES cells were cultured in complete neural stem cell medium (see section Cell culture ...
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bioRxiv - Cancer Biology 2023Quote: ... and dead cells were removed with the Dead Cell Removal Kit (Miltenyi Biotec, 130-090-101) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... LP cell suspensions were enriched for HLA-DR+ cells using anti-HLA-DR microbeads (Miltenyi Biotec) and LS MACS columns according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: B cells were eluted from PBMCs using a MACS Human B Cell isolation kit (Miltenyi Biotec). B cells were stained with rGP38 (IbAr10200 ...
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bioRxiv - Immunology 2023Quote: ... and total splenic B cells were purified using a negative B cell isolation kit (Miltenyi Biotec). Purity of enriched B cells was determined by flow cytometry ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells were negatively selected from PBMCs using CD4+ T cell isolation kit (Miltenyi Biotec) and cultured in RPMI 1640 media supplemented with 25 mM HEPES ...
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bioRxiv - Immunology 2024Quote: ... NKG2C+ NK cells were then selectively isolated as aNK cells using anti-PE microbeads (Miltenyi Biotec), while the flow-through ...
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bioRxiv - Immunology 2024Quote: NK cells were isolated from PBMCs using a NK cell negative selection kit from Miltenyi Biotec as per manufacturer’s instructions using LS columns (Miltenyi Biotec ...
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bioRxiv - Pathology 2020Quote: ... Dead Cell Removal Kit (Miltenyi Biotec) was used to remove the cells with low viability ...
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bioRxiv - Neuroscience 2023Quote: ... dead cell removal kit (Miltenyi Biotec) to purify single cells ...
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bioRxiv - Immunology 2022Quote: ... stem cell factor (SCF, Miltenyi Biotec), and thrombopoietin (TPO ...
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bioRxiv - Plant Biology 2020Quote: ... 20 mg of total protein in a volume of 5 ml were incubated with 50 μl of anti-GFP superparamagnetic MicroBeads (Miltenyi Biotec) for 1 h at 4 °C with gentle agitation ...
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bioRxiv - Cell Biology 2022Quote: ... 1 ml of protein extract was mixed with 50 µl of magnetic beads from µMACS™ GFP Isolation Kit (Miltenyi Biotec) and the mixture was incubated on ice while mild shaking ...
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bioRxiv - Cell Biology 2022Quote: ... aliquots of isolated mitochondria (1 µg total protein each) first had 100 ng mRNA spike-in control added (StemMACS eGFP mRNA #130-101-114, Miltenyi Biotec), and were then incubated with or without triton-x (1% v/v ...
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bioRxiv - Cell Biology 2019Quote: ... The MicroBeads/cell extracts mixture was then applied to the μColumn followed by gentle wash with the lysis buffer used above for protein extraction (Miltenyi Biotec). Pre-heated (95°C ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were detected with anti-TuMV coat protein antibody at a concentration of 1:1500 and Goat anti-Rabbit IgG (H+L)-HRP conjugate (Miltenyi Biotec) at a concentration of 1:10,000 ...
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bioRxiv - Cancer Biology 2022Quote: ... we separate the epidermal tissue from the dermis and then dissociated to single-cell suspensions by combining mechanical dissociation with enzymatic degradation of the extracellular adhesion proteins using Epidermis Dissociation Kit for Human (130-103-464, MACS Miltenyi Biotec). The dissociated cell suspension was strained with a 40μm filter (BD Falcon) ...
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bioRxiv - Immunology 2023Quote: ... Duplicate cultures were stimulated with or without a combined preparation of Peptivator Peptide Pools of the complete Spike protein and predicted immunodominant sequences (Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Immunology 2021Quote: ... and PepTivator® SARS-CoV-2 Prot_S Complete peptide pool (Miltenyi Biotec, 15 mers with 11 amino acid overlap) covering the entire SARS-CoV-2 S protein ...
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bioRxiv - Cancer Biology 2022Quote: ... Spleens were placed in 3 ml of PBS supplemented with 2% FBS in C tubes and dissociated using program m_spleen_01 on a gentleMACS Octo dissociator with heaters (Miltenyi Biotec). Dissociated tissue was passaged through a 70 μm cell strainer and centrifuged at 1500 rpm x 5 minutes ...
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bioRxiv - Immunology 2022Quote: ... 500 IU/ml IL-2 and 10 ng/ml IL-15 (all the above regents were from Miltenyi Biotec). The above transfection mix was added into NK cell culture seeded in six-well plates (3×106/well ...
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bioRxiv - Genomics 2023Quote: ... pre-coated in 1 mM EDTA and deposited into a 5 mL ice-cold of PEB buffer (PBS, pH 7.2, 0.5% BSA, 2 mM EDTA; dilution of MACS BSA Stock Solution [Miltenyi Biotec, 130-091-376] 1:20 with autoMACS® Rinsing Solution [Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: Naive B cells were purified from PBMCs using the MACS Human B Cell isolation kit (Miltenyi Biotec) and incubated with 25nM of each SARS-CoV-2 probe (RBD-mFc-BV650 ...
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bioRxiv - Systems Biology 2019Quote: ... 100 μl per 10 million cells of MACS dead cell removal beads (Miltenyi Biotec, 130-090-101) were added and incubated according to the product’s accompanying protocols ...
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bioRxiv - Biophysics 2019Quote: ... T cells were isolated from PBMCs with Pan T cell isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany), then were stimulated for 48⍰h with anti-CD3/anti-CD28 Dynabeads (Gibco by Thermo Fischer Scientific ...
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bioRxiv - Immunology 2019Quote: ... CTV-labeled T cells were then isolated using a Pan naïve T cell isolation kit (Miltenyi Biotec) according to manufacturer’s instructions and isolation purity (≥95% ...
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bioRxiv - Synthetic Biology 2021Quote: ... Primary human T cells were purified from PBMCs by Pan T Cell Isolation Kit (Miltenyi Biotec, Germany). Purified T cells were stimulated with anti-CD3/CD28 Dynabeads (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... NK cells were isolated from PBMCs by negative depletion with human NK cell Isolation Kit (Miltenyi Biotec). Cells were cultured in RPMI 1640 medium supplemented with GlutaMAX (Gibco) ...
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bioRxiv - Cancer Biology 2022Quote: ... RBC lysed and dead cells were separated (using dead cell removal kit, Miltenyi Biotec, 130-090-101). The live cells were then resuspended in a sort buffer to inject 1 million cells per B6 MMTV-PyMT mouse retro-orbitally.
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bioRxiv - Systems Biology 2022Quote: ... and MM cells were enriched by CD138-positive selection with magnetic activated cell separation microbeads (Miltenyi Biotec). MM cells were cultured in RPMI containing 100 U/ml penicillin and 100 ug/ml streptomycin ...
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bioRxiv - Immunology 2022Quote: ... and further enriched for memory cells by depletion of naïve T cells using CD45RA beads (Miltenyi Biotec), prior to lectin staining and sorting as described further below ...
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bioRxiv - Immunology 2020Quote: ... while bone marrow (BM) cells were depleted of T cells by using CD90.2 magnetic beads (Miltenyi Biotec). C57BL/6 (syngeneic) ...
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bioRxiv - Immunology 2019Quote: ... Total B-cells were isolated by negative selection with the Memory B-cell Isolation Kit (Miltenyi Biotec). Memory-enriched B cells fractions were isolated by negative selection following incubation of total ...
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bioRxiv - Biochemistry 2021Quote: ... Splenic T cells were purified using T cell isolation kits and AutoMacs magnetic separation system (Miltenyi Biotec) and approximately 90% of the purified cells displayed a naïve phenotype as previously described19 ...
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bioRxiv - Immunology 2019Quote: ... CD4+ CD8+ T cell subsets were isolated via magnetic separation using Pan T cell kit (Miltenyi Biotec). Effector-T-cells (CD25− ...
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bioRxiv - Immunology 2020Quote: ... TCRβ+CD4+CD138− cells were isolated from TCRβ+CD138− cells using the CD4 (L3T4) MicroBeads (Miltenyi Biotec), and unbound cells were identified as TCRβ+CD8+CD138− cells.
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bioRxiv - Cell Biology 2021Quote: ... dissociated cells were incubated with CD90 microbeads (Miltenyi Biotec, 130-096-253; 1 μl per 107 cells) to enrich CD90+ NPCs.
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bioRxiv - Biochemistry 2021Quote: ... The CD73 positive cells were collected as photoreceptor cells using an autoMACS Pro Separator (Miltenyi Biotec, USA.).
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bioRxiv - Immunology 2021Quote: ... Naïve CD4 T cells were isolated using the Naïve CD4 T Cell Isolation Kit II (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Murine CD4+ T cells were purified using the MACS CD4+ T cell negative selection kit (Miltenyi Biotec) and naïve CD4+ T cells from WT ...