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Citations for Miltenyi Biotec :
201 - 250 of 645 citations for Complement Factor B CFB Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... The antibody cocktails included anti-mouse antibodies from Miltenyi Biotec: CD44-FITC (clone IM7.8.1) ...
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bioRxiv - Cancer Biology 2021Quote: ... The purity of isolated B cells was over 95% as assessed by flow cytometry analysis of PE-CD19+ cells (Miltenyi Biotec) 87,88.
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bioRxiv - Immunology 2020Quote: Mouse CB7Bl/6 MZ and FO B cells were isolated from mouse spleen cell suspension by anti-CD19 exclusion using a kit #130-100-366 (Miltenyi Biotec). Cells were fluorescently stained with rabbit αβ-Actin mAb (SP124 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Human B cells were isolated from PBMC collected from human volunteer donors by magnetic-activated cell sorting using the B cell Isolation Kit II for human cells from Miltenyi Biotec Inc (Auburn ...
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bioRxiv - Immunology 2021Quote: ... Samples were then transferred to 15 mL conical tubes and non-memory B cells were depleted using the AutoMACS Magnetic Sorter (Miltenyi Biotec) “deplete” program ...
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bioRxiv - Molecular Biology 2023Quote: ... primary B cells were isolated from Rif1FH/FH and C57BL/6 (Rif1WT/WT) mice spleens using anti-CD43 MicroBeads (Miltenyi Biotec) and expanded in complete RPMI containing 5 µg/ml LPS (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... For two donors the B cells were isolated using MACS cell sorting with anti-human CD19 microbeads and MS columns from Miltenyi Biotec, according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... (#130-101-638, Miltenyi Biotec for B cells, #130-100-777, Miltenyi Biotec for dendritic cells, #130-092-657, Miltenyi Biotec for natural killer cells ...
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bioRxiv - Immunology 2023Quote: ... B cells were isolated from PBMCs through two rounds of magnetic enrichment using the human pan B cell isolation kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... staining and single-cell sorting of PvAMA1-specific IgG+ MBCs were performed as follows: B cells were enriched using immunomagnetic positive selection with anti-CD19 magnetic MACS beads (Miltenyi Biotec) and stained with mouse anti-human CD20 (PE-Cy5.5 ...
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bioRxiv - Immunology 2023Quote: ... B cells were first enriched from the splenocyte suspension by MACS (magnetic activated cell sorting) using the Pan B cell Isolation Kit (Miltenyi Biotec), followed by staining for FACS (fluorescence activated cell sorting) ...
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bioRxiv - Biochemistry 2021Quote: Memory B cells were isolated from Peripheral blood mononuclear cells (PBMCs) derived from blood samples from COVID-19 convalescent patients using a Human Memory B cell isolation kit (Miltenyi Biotec, GE). Small pools of purified Memory B cells were seeded into 384-well plates on irradiated CD40L-expressing feeder cells for differentiation into plasma cells as described previously (41) ...
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bioRxiv - Immunology 2022Quote: ... B cells were isolated using either the StraightFrom™ Buffy Coat CD19 Microbead Kit or the Human B cell Isolation Kit II (both from Miltenyi Biotec), following the manufacturer’s protocols ...
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bioRxiv - Immunology 2020Quote: ... cells were resuspended in 480 μL staining buffer before adding 120 μL anti-mouse IgG2a/b MicroBeads (Miltenyi Biotec, Bergisch Gladbach, Germany) for an incubation period of 15 min ...
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bioRxiv - Cancer Biology 2023Quote: Splenic B cells from mice immunized with SRBC for 10 days were pre-enriched by B220 MicroBeads (Miltenyi Biotec; 130-049-501) followed by flow sorting ...
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bioRxiv - Immunology 2021Quote: B cells from spleens and lymph node of naïve mice were purified by magnetic separation using a pan-B cell isolation kit (Miltenyi Biotec #130-095-813), then incubated overnight with 30 μg/ml LPS (Sigma #L2630 ...
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bioRxiv - Immunology 2022Quote: ... B cells were depleted from OTII splenocytes by magnetic sorting using CD45R (B220) MicroBeads according manufacturer’s instructions (Miltenyi Biotec cat. 130-049-501). For experiments involving cell division analysis ...
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bioRxiv - Bioengineering 2022Quote: Omicron RBD-specific memory B cells were isolated from pre-enriched memory B cells by magnetic positive selection according to the manufacturer’s protocol (Miltenyi Biotec, 130-095-838). Briefly ...
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bioRxiv - Systems Biology 2022Quote: ... and T and B cells were depleted by using CD19 and CD3 MicroBeads (Miltenyi Biotec Cat#130-097-055 and #130-097-043) to enrich for myeloid cells ...
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bioRxiv - Immunology 2022Quote: B cells from PBMCs of uninfected controls or of COVID-19 convalescent individuals were enriched using the pan-B-cell isolation kit according to manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were subsequently stained in FACS buffer (PBS + 2% FCS + 1mM EDTA ...
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bioRxiv - Molecular Biology 2023Quote: ... natural killer cells and monocytes were isolated from PBMCs by negative selection and magnetic sorting using cocktails of biotin-conjugated antibodies against non-target cells (#130-096-535,Miltenyi Biotec for T cells, (#130-101-638, Miltenyi Biotec for B cells, #130-100-777, Miltenyi Biotec for dendritic cells ...
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bioRxiv - Immunology 2024Quote: ... splenic P14 TM were enriched at indicated time points after LCMV Arm by removal of APC- or PE-labeled CD4+ T and CD19+ B cells with anti-APC/PE magnetic microbeads (Miltenyi Biotec & STEMCELL Technologies) and 5×103 P14 TM were transferred i.v ...
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bioRxiv - Molecular Biology 2023Quote: ... PBMCs were activated using anti-CD3 antibody and anti-CD28 antibody (Miltenyi Biotec). 24 hr post activation ...
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bioRxiv - Cancer Biology 2022Quote: B cells were purified by negative selection from single-cell suspensions from spleen using magnetic separation B cell isolation kit (Miltenyi Biotec 130-090-862). Purified cells were cultured for 4 days in RPMI (Corning ...
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bioRxiv - Genetics 2021Quote: ... CD14+ monocytes and CD19+ B cells were isolated from human PBMCs using the Human CD3+ T Cell Isolation kit (Miltenyi Biotec,130-050-101), Human CD14+ monocytes Isolation kit (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells and CD19+ B cells were primarily sorted from PBMCs of convalescents and vaccinees by CD4 and CD19 MicroBeads (Miltenyi Biotec, Bergisch Gladbach, Germany), respectively ...
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bioRxiv - Molecular Biology 2023Quote: B cells were purified from red blood cell-lysed single-cell suspensions of mouse spleens by magnetic negative selection using a B Cell Isolation Kit (Miltenyi Biotec, 130-090-862). B cells (3x105 per well in a 12-well plate ...
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bioRxiv - Genetics 2020Quote: ... and CD28 antibodies (Miltenyi Biotec) and 10ng/ml recombinant IL-2 in complete RPMI ...
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bioRxiv - Genetics 2020Quote: ... and CD28 antibodies (Miltenyi Biotec) as described earlier ...
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bioRxiv - Neuroscience 2020Quote: ... CD63 biotinylated antibody (Miltenyi Biotec, Cat# 130-108-922 ...
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bioRxiv - Molecular Biology 2022Quote: Healthy and CLL splenic B cells were isolated from one-year-old WT and Eμ–TCL1 mice by positive B-cell selection with CD19 magnetic beads (CD19 MicroBeads, Miltenyi Biotec, cat. 130-121-301). Briefly ...
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bioRxiv - Immunology 2022Quote: ... an isotype control antibody was used (1:50 dilution, REA control antibody, Miltenyi Biotec, Germany). Samples were analysed using a flow cytometer (Becton Dickinson FACSCalibur) ...
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bioRxiv - Cancer Biology 2019Quote: ... Naïve mouse B-cells were isolated from the spleen of 7–10 weeks old wild type or c-mycf/f mice with the B-cell isolation kit (MACS Miltenyi Biotec Cat. no. 130-090-862). After purification ...
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bioRxiv - Cell Biology 2024Quote: ... supernatant was removed and pellet was resuspended in CMF-B (uptake) or 1X PBS (EdU labeling) then passed through a 70 µm filter (Miltenyi Biotec MACS SmartStrainers, 130-098-462) to remove aggregates.
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bioRxiv - Immunology 2019Quote: The following antibodies from Miltenyi Biotec were used for flow cytometry ...
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bioRxiv - Cancer Biology 2020Quote: Fluorescent antibodies against Gr1 (Miltenyi Biotec), CD11b (BD) ...
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bioRxiv - Molecular Biology 2020Quote: ... Anti-DYKDDDDK-HRP antibodies (Miltenyi Biotec) were used to immunostain Tj-TR-3xFlag bands ...
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bioRxiv - Developmental Biology 2023Quote: ... CD19 and CD45R antibodies (Miltenyi Biotec) were added to the mixture and the cells were further incubated on ice for ten minutes ...
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bioRxiv - Immunology 2022Quote: ... an anti-biotin–PE antibody (Miltenyi Biotec) was exclusively used as described 26 ...
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bioRxiv - Developmental Biology 2021Quote: ... The antibodies (Miltenyi Biotec, Bergisch Gladbach, Germany) used in this study included anti-human CD34-FITC (130-098-142) ...
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bioRxiv - Immunology 2020Quote: ... All antibodies were purchased from Miltenyi Biotec, Invitrogen or BD Biosciences.
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bioRxiv - Cell Biology 2022Quote: ... All antibodies were purchased from Miltenyi Biotec
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bioRxiv - Immunology 2023Quote: ... an anti-biotin–PE antibody (Miltenyi Biotec) was exclusively used ...
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bioRxiv - Immunology 2023Quote: ... a biotinylated anti-NKG2A antibody (Miltenyi Biotec) and Anti-Biotin Microbeads (Miltenyi Biotec ...
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bioRxiv - Molecular Biology 2020Quote: ... anti SSEA1 monoclonal antibody DSHB) and then incubated with a microbead-linked donkey anti-mouse IgM antibody (Miltenyi Biotec). Then ...
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bioRxiv - Microbiology 2019Quote: ... or IFN-γ detection antibody (Miltenyi Biotec, Germany). After washing the cells ...
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bioRxiv - Bioengineering 2020Quote: ... tagged with a CD34-PE antibody (Miltenyi Biotec), and purified on a fluorescence-activated cell sorting (FACS ...
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bioRxiv - Neuroscience 2021Quote: ... The CD31-FITC antibody (Miltenyi Biotec, Auburn, CA) was added to the cell suspension at a dilution of 1:50 ...
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bioRxiv - Immunology 2022Quote: ... Then the following antibodies (all from Miltenyi Biotec) were used ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary CD133 antibody (Miltenyi Biotec, 130-090-422) was diluted 1:25 in Antibody Diluent with Background Reducing Components (Dako ...