Labshake search
Citations for Miltenyi Biotec :
1 - 50 of 653 citations for CD28 Human Cynomolgus HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2020Quote: ... and human anti-CD28 (clone 15E8, Miltenyi Biotec) and cultured at 37°C in a 5% CO2 incubator.
-
bioRxiv - Immunology 2019Quote: ... Human Fc blocking reagent (Miltenyi Biotec) and 2.4G2 mouse blocking reagent were used in all human and mouse stains respectively.
-
bioRxiv - Microbiology 2020Quote: ... human Fc blocking reagent (MACS Miltenyi Biotec) was added to this blocking step ...
-
bioRxiv - Immunology 2023Quote: ... and stimulated with Dynabeads Human T-Activator CD3/CD28 (Miltenyi Biotec 111.31D) for 48 hours at 37°C ...
-
bioRxiv - Immunology 2024Quote: ... and human Treg expansion kit anti-CD3/CD28 beads (Miltenyi Biotec/Invitrogen), using x-vivo 15 cell medium (Lonza ...
-
bioRxiv - Cancer Biology 2022Quote: ... in FACS buffer containing 1% human Fc Block (Miltenyi Biotec) for 30 mins at 4ºC and were then washed three times ...
-
bioRxiv - Cancer Biology 2023Quote: ... cells were suspended in human FC block (Miltenyi Biotec Inc., FLP3330) and kept on ice for 30 minutes ...
-
bioRxiv - Immunology 2022Quote: ... for 20 minutes at 4C with anti-human Fc Block (Miltenyi Biotec). Cells were incubated with DAPI viability dye (Thermo ...
-
bioRxiv - Genetics 2020Quote: ... and CD28 antibodies (Miltenyi Biotec) and 10ng/ml recombinant IL-2 in complete RPMI ...
-
bioRxiv - Genetics 2020Quote: ... and CD28 antibodies (Miltenyi Biotec) as described earlier ...
-
bioRxiv - Immunology 2020Quote: ... Fc receptor were blocked with human FcR Blocking Reagent (120-000-442, Miltenyi Biotec) and anti-CD16/32 (clone 2.4G2 ...
-
bioRxiv - Microbiology 2021Quote: ... After a 15 minute-incubation step with human Fc blocking reagent (MACS Miltenyi Biotec), cell surface markers were detected by a 30 minute-incubation at 4°C with 4 µg/mL Pacific Blue-conjugated mouse anti-CD14 (clone 63D3 ...
-
bioRxiv - Bioengineering 2023Quote: ... Germany]) and activated using Dynabeads™ Human T-Activator CD3/CD28 Beads and 50 U/mL IL-2 (Miltenyi Biotec).
-
bioRxiv - Bioengineering 2023Quote: ... and anti-CD28 (MACS, Miltenyi Biotec) coated plate in 1 µg of each antibody in 1 ml PBS per 24-well plate for 2 h at room temperature ...
-
bioRxiv - Immunology 2021Quote: ... NK cells highly enriched from normal cynomolgus macaque (Macaca fascicularis) blood samples using a CD3 depletion kit (Miltenyi Biotec), were used as effector cells ...
-
bioRxiv - Immunology 2022Quote: ... A Fc receptor (FcR) blocking step was performed using FcR Blocking Reagent Human (Miltenyi Biotec) before cell surface antibody staining ...
-
bioRxiv - Immunology 2021Quote: NK cell effectors were enriched from normal cynomolgus macaque (Macaca fascicularis) blood samples using a CD3 depletion Kit (Miltenyi Biotec). Target and effector cells were plated at E:T ratio of 2:1 in a 96 well round bottom plate ...
-
bioRxiv - Bioengineering 2021Quote: ... The general surface marker composition for the sEVs from the three cell sources (HEK293, cbMSC, THP1) was assessed by a multiplex bead-based flow cytometry assay (MACSPlex Exosome Kit, human, Miltenyi Biotec) by using an optimized workflow as described previously.[27] In brief ...
-
bioRxiv - Microbiology 2021Quote: ... CD2/CD3/CD28 activation beads (Miltenyi Biotec, Germany) or plastic-adhered anti-CD3 mAb plus soluble anti-CD28 mAb for 2-3 days ...
-
A modular CRISPR screen identifies individual and combination pathways contributing to HIV-1 latencybioRxiv - Microbiology 2022Quote: ... and CD28 beads (Miltenyi Biotec; 130-091-441) for 2 days and grown in RPMI-1640 medium (Thermo Fisher Scientific ...
-
bioRxiv - Bioengineering 2024Quote: ... and anti-CD28 antibodies (clone: CD28.2; Miltenyi Biotec). Forty-eight hours before transduction and on the day of transduction ...
-
bioRxiv - Immunology 2021Quote: ... The CD28 MicroBead Kit (130-093-247; Miltenyi Biotec) was used for isolation of CD4+CD28null T-cells from PBMC ...
-
bioRxiv - Molecular Biology 2023Quote: ... Loaded CD2/CD3/CD28 MACSiBeads (#130-091-441, Miltenyi Biotec) were added in a 1:2 bead-to-cell-ratio according to manufacturer’s instructions and incubated for 3 days at 37°C ...
-
bioRxiv - Immunology 2022Quote: ... The Fc blocked cells were stained with 1:200 diluted anti-human CD45-APC (Cat. #130-113-114, Miltenyi Biotec) for 20 min at 4 degree Celsius in dark ...
-
bioRxiv - Immunology 2019Quote: ... (at 1:20-1:100 dilutions depending on the antibody) in the presence of human Fc receptor blocking reagent (1:10) (Miltenyi Biotec) for 15 min at 4°C ...
-
bioRxiv - Cell Biology 2022Quote: ... samples were saturated with PBS containing 1% BSA and 1% FCS and human FcR-blocking reagent (Miltenyi Biotec, 130-059-901) for 15 minutes at 4°C prior to labelling using an anti-human CD95-APC antibody (clone DX2 ...
-
bioRxiv - Genetics 2020Quote: ... and CD28 antibodies (bead-to-cell ratio 1:2, Miltenyi Biotec). Resting or stimulated CD4 T cells were nucleofected with the MPRA plasmid library using at least 6 technical replicates for each sample ...
-
bioRxiv - Genetics 2020Quote: ... and CD28 antibodies (bead-to-cell ratio 1:2, Miltenyi Biotec) and IL-2 (10ng/ml ...
-
bioRxiv - Microbiology 2023Quote: ... and activated with CD2/CD3/CD28 magnetic beads (Miltenyi Biotec, Germany) for 48 hr ...
-
bioRxiv - Immunology 2019Quote: ... cells were activated with T Cell TransAct CD3/CD28 (Miltenyi Biotec, Germany) at 1:200 concentration 24h after electroporation and the next day the supernatant was removed to withdraw beads ...
-
bioRxiv - Immunology 2022Quote: ... and stimulated with CD3/CD28 MACSiBead Particles (Miltenyi Biotec, Bergisch Gladbach, Germany) in a 1:1 ratio for 2 h at room temperature ...
-
bioRxiv - Cancer Biology 2021Quote: ... and stimulated with particles coated with anti-CD3/anti-CD28 antibodies (Miltenyi Biotec) at a cell-to-bead ratio of 1:2 ...
-
bioRxiv - Immunology 2020Quote: ... T cells were activated by injection of anti-CD3/CD28 beads (Miltenyi Biotec) at a ratio of 8:1 beads:T cell followed by injection of oligomycin (2μM ...
-
bioRxiv - Genomics 2023Quote: ... at 0.1ug/ul with either commercially biotinylated Anti-CD28 (Clone 15E8, Miltenyi Biotec), Anti-ICOS (Clone ISA-3 ...
-
bioRxiv - Molecular Biology 2023Quote: ... PBMCs were activated using anti-CD3 antibody and anti-CD28 antibody (Miltenyi Biotec). 24 hr post activation ...
-
bioRxiv - Biophysics 2023Quote: T cells were activated using αCD3/CD28 beads (T Cell TransActTM; Miltenyi Biotec), according to the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2020Quote: ... Fc receptor block (Miltenyi Biotec Inc) was added and incubated on ice-water bath for 10 min ...
-
bioRxiv - Molecular Biology 2020Quote: ... Fc receptor blocker (Miltenyi Biotec Inc) was added and ice-water bath was used for 10 minutes ...
-
bioRxiv - Immunology 2024Quote: ... containing Fc receptor blocker (Miltenyi Biotec) and incubated for 10 minutes at 4°C ...
-
bioRxiv - Immunology 2020Quote: ... anti-CD28 APC-Vio770 (clone REA612, Miltenyi Biotec, Cat. 130-116-506, 1:100), anti-CD45RA BV421 (clone HI100 ...
-
bioRxiv - Cancer Biology 2022Quote: ... the Fc receptor was blocked (Miltenyi Biotec) and cells were stained with the following antibodies for 30 minutes at 4°C ...
-
bioRxiv - Microbiology 2020Quote: ... Cells were then restimulated using CD2/CD3/CD28 flow cytometry-compatible stimulation beads (Miltenyi Biotec) in complete RPMI-1640 supplemented with 80 U/ml IL-2-IS (Miltenyi Biotec ...
-
bioRxiv - Cancer Biology 2023Quote: ... The T cells were stimulated with anti-CD3/CD28 beads (x 25 dilution; Miltenyi Biotec) in the presence or absence of recombinant mouse TGF-β1 (5 ng/ml ...
-
bioRxiv - Cancer Biology 2022Quote: ... anti-CD28 antibody (15E8; 0.5µg/mL) (Miltenyi Biotec, Cat# 130-093-387 and 130-093-375) and IL-2 (100 U/ml ...
-
bioRxiv - Cancer Biology 2022Quote: ... anti-CD28 antibody (15E8; 0.5µg/mL) (Miltenyi Biotec, Cat# 130- 093-387 and 130-093-375), and IL-2 (100 U/mL ...
-
bioRxiv - Immunology 2021Quote: ... Cells were restimulated with anti-CD2/anti-CD3/anti-CD28 (T cell activation beads, Miltenyi Biotec) to achieve a 1:1 bead/cell ratio in accordance with the manufacturer’s instructions for cell expansion ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... and cultured in the presence of 3 μg/mL anti-CD28 mAb (clone: 15E8, Miltenyi Biotec), 25 U/mL human IL-7 (Miltenyi Biotec ...
-
bioRxiv - Cancer Biology 2020Quote: ... Samples were incubated with Fc blocking reagent (Miltenyi Biotec) for 10 minutes at room temperature before the addition of monoclonal antibodies for 30 minutes at 4°C ...
-
bioRxiv - Immunology 2022Quote: ... cells were resuspended in Fc receptor blocker (Miltenyi Biotec) diluted in PBS/2% BSA and incubated on ice for 10 min ...
-
bioRxiv - Immunology 2022Quote: ... followed by incubation with Fc receptor blocker (Miltenyi Biotec) for 15 min on ice ...