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Citations for Miltenyi Biotec :
201 - 250 of 300 citations for CD14 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... CD4+ or CD14+ cells were positively selected using either CD4 or CD14 Microbeads loaded onto MACS separation columns according to manufacturer’s instructions (Miltenyi Biotec). CD4+ PBMCs were grown in the above cell media supplemented with 5ng/ml IL-2 (Peprotech ...
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bioRxiv - Cell Biology 2021Quote: ... 0.5% heat-inactivated fetal calf serum (FCS) at pH 7.4 and magnetically sorted with using magnetic microbeads coated with antibodies directed against CD14 (Miltenyi Biotec). Monocytes were then seeded on plastic at 2×106 cells/well in six-well plates in RPMI 1640 (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... Fresh tumor tissue from NSCLC patients were enzymatically digested and single-cell suspensions were stained with CD14 microbeads (Miltenyi Biotec) and positively selected using AutoMACS ProSeparator (Miltenyi Biotec) ...
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bioRxiv - Microbiology 2021Quote: ... PBMCs were isolated using Ficoll-Hypaque density centrifugation and monocytes were positively selected using anti-CD14 microbeads (MACS Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... CD14+ cell isolation was done using immunomagnetic bead selection according to manufacturer’s instructions to achieve 95–99% purity (Miltenyi Biotec). CD14+ monocytes were cultured in RPMI cell medium supplemented with 10% FCS ...
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bioRxiv - Biophysics 2021Quote: ... 0.5% heat-inactivated Fetal Calf Serum (FCS) at pH 7.4 and magnetically sorted with magnetic microbeads coated with antibodies directed against CD14 (Miltenyi Biotec). Monocytes were then seeded on glass coverslips at 1.5 × 106 cells/well in six-well plates in RPMI 1640 (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... Pure monocytes (MO) were then isolated from PBMCs by positive selection with magnetic CD14 MicroBeads (Miltenyi Biotec, Bergisch Gladbach, Germany). Purified monocytes were resuspended in Roswell Park Memorial Institute (RPMI ...
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bioRxiv - Immunology 2020Quote: ... CD14+ monocytes were isolated from peripheral blood mononuclear cells (PBMC) using magnetic bead selection (Miltenyi Biotec, San Diego, CA, USA). To differentiate cells into macrophages ...
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bioRxiv - Immunology 2022Quote: ... recombinant human IL-2 (100 IU/ml, Miltenyi Biotec), PHA (0,25 µg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 50ng/ml recombinant human SCF (Miltenyi Biotec), recombinant human Flt3-ligand (Miltenyi Biotec) ...
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bioRxiv - Immunology 2019Quote: ... Donors were screened for the presence of KIR2DS4 with the mAb JJC11.6 (Miltenyi Biotec). 105 resting NK cells were mixed at 1:1 with 221 cell lines or peptide loaded target cells in presence of 1 μl BV421 anti-CD107a mAb for 2 hr at 37°C (H4A3 ...
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bioRxiv - Immunology 2019Quote: ... Cells were then stained with mAbs to KIR2DS4 (Miltenyi Biotec, JJC11.6, 130-099-963) and KIR2DL1/S1 (EB6 ...
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bioRxiv - Immunology 2021Quote: ... the cells were resuspended in PBS containing 2% FCS and 2mM EDTA and stained with the extracellular mix using APC-Vio770 anti-CD14 (clone REA599) from Miltenyi Biotec and used at 1/100 ...
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bioRxiv - Immunology 2019Quote: CD4+ and CD14+ cells were enriched from the PBMCs of 3 healthy donors via magnetic separation using the corresponding MicroBeads (Miltenyi Biotec). The CD14+ fraction was cryopreserved in liquid nitrogen ...
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bioRxiv - Immunology 2019Quote: Human monocytes were purified from peripheral blood mononuclear cells (PBMCs) from healthy donors by positive immunoselection with anti-CD14-conjugated MACS beads (Miltenyi Biotec). Monocytes were also purified from PBMCs from either HLA-A2 WT NOD2 donors or HLA-A2 homozygous mutant NOD2 Crohn’s patients ...
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bioRxiv - Immunology 2021Quote: ... were obtained by density gradient centrifugation and monocytes were subsequently purified by immunomagnetic separation using anti CD14-conjugated magnetic microbeads (Miltenyi Biotec) according to the manufacture’s protocol and as previously published (23) ...
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bioRxiv - Immunology 2022Quote: ... CD14+ monocytes were isolated from PBMC from healthy subjects by positive selection withanti-CD14 magnetic labeling beads and an isolation column (Miltenyi Biotec). Monocytes were resuspended in RPMI 1640 medium (Gibco ...
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bioRxiv - Immunology 2022Quote: ... and monocytes were purified with anti-CD14 magnetic beads from PBMCs immediately after isolation as recommended by the manufacturer (Miltenyi Biotec) 85 ...
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bioRxiv - Immunology 2019Quote: ... washed and either used for RNA extraction or for isolation of monocytes by positive selection (CD14) with magnetic beads (130-050-201, Miltenyi Biotec).
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bioRxiv - Immunology 2019Quote: ... Monocyte isolation was performed by gradient centrifugation on Percoll (Pharmacia, Uppsala, Sweden) following by magnetic-activated cell separation sorting using human CD14 Microbeads (Miltenyi Biotec). Freshly isolated CD14+ monocytes were cultured for 7-8 days to differentiate into macrophages in IMDM medium supplemented with 10% FCS ...
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bioRxiv - Immunology 2019Quote: Human monocytes were purified from healthy donor peripheral blood mononuclear cells (PBMCs) by positive immunoselection with anti-CD14-conjugated MACS beads (Miltenyi Biotec). moDCs were obtained by culturing monocytes for 5 days with IL-4 and GM-CSF (Peprotech) ...
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bioRxiv - Genomics 2021Quote: ... with fluorescent APC-conjugated anti-CD14 and PE-conjugated anti-CD16 antibodies (Cat#130-091-243 and Cat #130-091-245, respectively, Miltenyi Biotec). Samples were then analyzed on a MACSQuant Analyzer 10 benchtop flow cytometer (Miltenyi Biotec).
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bioRxiv - Immunology 2023Quote: ... through peripheral blood mononuclear cell isolation by Ficoll density gradient centrifugation and magnetic separation using CD14 MicroBeads (130-050-201, Miltenyi Biotec), according to manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... through depletion of non-target cells by anti CD14/ CD15/CD16/CD19/CD34/CD36/CD56/CD123/CD235a biotin-conjugated monoclonal antibodies MicroBeads Kit (Miltenyi Biotec), with the use of a LS column ...
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bioRxiv - Immunology 2024Quote: ... and monocytes were purified with anti-CD14 magnetic beads from PBMCs immediately after isolation as recommended by the manufacturer (Miltenyi Biotec)53 ...
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bioRxiv - Microbiology 2020Quote: ... 5 million units per kg recombinant mouse IFNα (Miltenyi Biotec) were injected intravenously ...
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bioRxiv - Immunology 2022Quote: ... Recombinant human transforming growth factor β1 (premium grade, Miltenyi Biotec) was diluted and used at the indicated concentrations.
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bioRxiv - Neuroscience 2023Quote: ... and human recombinant TGF-β1 50 ng/mL (Miltenyi Biotec).
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bioRxiv - Genomics 2021Quote: ... CD14+ monocytes were isolated from PBMCs by a magnetic cell separation system (MACS) using anti-human CD14 antibody attached to microbeads (Miltenyi Biotec #130050201) on an LS column (Miltenyi Biotec #130-042-401 ...
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bioRxiv - Genomics 2021Quote: ... CD4+ T cells were then isolated from CD14+ monocyte-free PBMCs using anti-human CD4 antibody attached to microbeads (Miltenyi Biotec #130045101) on a new LS column ...
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bioRxiv - Immunology 2020Quote: Human monocytes were isolated from peripheral blood mononuclear cells from healthy donors (convention n°7828, Etablissement Français du Sang, Marseille, France) following CD14 selection using MACS magnetic beads (Miltenyi Biotec, Bergisch, Germany) as previously described (19) ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary human monocytes and CD3+ T cells were isolated from PBMCs using CD14 MicroBeads™ and pan T cell isolation kit (Miltenyi Biotec), respectively ...
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bioRxiv - Pathology 2021Quote: ... Human recombinant OSM was added at 100 ng/ml (Miltenyi Biotec). Cells were cultured for 14 days at 37°C in 5% CO2 atmosphere ...
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bioRxiv - Developmental Biology 2022Quote: ... 20 ng/mL human recombinant FGF-2 (all from Miltenyi Biotec)) ...
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bioRxiv - Developmental Biology 2024Quote: ... 20 ng/mL human recombinant FGF-2 (all from Miltenyi Biotec)) and growth factor-reduced Matrigel (Corning ...
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bioRxiv - Immunology 2024Quote: ... with 30 U/ml of recombinant human IL-2 (Miltenyi Biotec). T cells were cultured in AIMV (ThermoFisher Scientific ...
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bioRxiv - Immunology 2019Quote: ... 130-096-793), CD20-VioGreen (LT20, cat.: 130-113-379), CD14-VioGreen (TÜK4, 130-113-153) (all from Miltenyi Biotec, Bergisch Gladbach, Germany);
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bioRxiv - Immunology 2022Quote: ... The pellet was resuspended in 0.5 mL DPBS with 0.5 % BSA and labeled with 50 μL anti-CD14 microbeads (Miltenyi Biotec, catalog# 130-050-201). Cells were labeled for at least 20 minutes at 4 ° C and CD14+ cells were isolated by magnetic column (Miltenyi Biotec ...
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Dendritic cells focus CTL responses toward highly conserved and topologically important HIV epitopesbioRxiv - Immunology 2020Quote: ... PBMC were thawed and monocytes and peripheral blood lymphocytes (PBL) were separated using human anti-CD14 Ab-coated microbeads to positively select monocytes (Miltenyi Biotec, Auburn, CA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Isolated PBMCs were then immediately used for the collection of CD14+ monocytes by positive selection using a Miltenyi bead-based protocol following manufacturer’s instructions (Miltenyi Biotec, Bergisch Gladbach, Germany). After assessing cell viability and numbers ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Human CD14+ monocytes were isolated from blood using Lymphoprep density centrifugation (STEMCELL, Melbourne, Australia) followed by CD14+ MACS magnetic bead separation (Miltenyi Biotec, Sydney, Australia). The isolated monocytes were differentiated for 7 days in Iscove’s Modified Dulbecco’s Medium supplemented with 10% FBS ...
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bioRxiv - Immunology 2020Quote: Monocytes were purified from freshly thawed PBMC using CD14 antibodies conjugated to magnetic microbeads per the manufacturer’s recommendations (Miltenyi Biotec, San Diego CA). Magnetically bound monocytes were washed and eluted for collection ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5% heat-inactivated Fetal Calf Serum (FCS) at pH 7.4 and monocytes were magnetically sorted with magnetic microbeads coupled with antibodies directed against CD14 (Miltenyi Biotec #130-050-201). Monocytes were then seeded on glass coverslips at 1.5×106 cells/well in six-well plates in RPMI 1640 (Gibco ...
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bioRxiv - Immunology 2024Quote: ... to obtain monocyte-derived macrophages which were purified with magnetic bead separation (CD14 negative isolation protocol, Monocyte Isolation Kit II. Miltenyi Biotec, Auburn, CA). Isolated CD14+ monocyte-derived macrophages (MDMs ...
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bioRxiv - Immunology 2020Quote: ... cells were blocked at 37C for 15 minutes with 0.5ug/mL anti-CD40 mAb (Miltenyi Biotec).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and cultured in the presence of 3 μg/mL anti-CD28 mAb (clone: 15E8, Miltenyi Biotec), 25 U/mL human IL-7 (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2020Quote: ... Once counted CD14+ human monocytes were isolated from PBMCs using magnetic labeling (Monocyte Isolation Kit II, Cat. 130-091-153, Miltenyi Biotec, San Diego, CA) and magnetic columns as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... lineage kit for depletion of hematopoietic and erythrocyte precursor cells (CD2, CD3, CD11b, CD14, CD15, CD16, CD19, CD56, CD123, and CD235a; Miltenyi Biotec, Birgisch Gladbach, Germany), endothelial cells (CD31 ...
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bioRxiv - Genetics 2021Quote: ... CD14+ monocytes and CD19+ B cells were isolated from human PBMCs using the Human CD3+ T Cell Isolation kit (Miltenyi Biotec,130-050-101), Human CD14+ monocytes Isolation kit (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... Peripheral blood mononuclear cells (PBMCs) were purified using density gradient centrifugation over Ficoll-Paque and monocytes isolated with subsequent CD14 MACS sorting (Miltenyi Biotec, Bergisch Gladsbach, Germany) as described previously [22 ...