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Citations for Miltenyi Biotec :
251 - 300 of 448 citations for AKT1 2 3 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... and in presence of 33 U/ml human IL-2 (premium-grade, Miltenyi Biotec).
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bioRxiv - Immunology 2020Quote: ... and 50 U/ml of human IL-2 IS (Miltenyi Biotec, #130-097-142) for primary T cells ...
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bioRxiv - Immunology 2021Quote: ... then positively selected using anti-ACSA-2 MicroBeads (Miltenyi Biotec, Cat# 130-097-678). Total RNA was prepared using TRI Reagent (Millipore-Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... astrocyte ACSA-2+ cell sorting was performed using an autoMACS Pro Separator (Miltenyi Biotec). After isolation ...
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bioRxiv - Neuroscience 2023Quote: ... followed by ACSA-2 MicroBeads (cat # 130-097-678, MACS Miltenyi Biotec, Auburn, CA) for 15 min ...
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bioRxiv - Microbiology 2023Quote: ... Cells were stimulated with IL-2 (10 ng/ml) (Miltenyi Biotec #130-097-745) and with anti-CD3/CD28 beads (Gibco #11132D ...
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bioRxiv - Developmental Biology 2024Quote: ... 2-photon imaging was also performed with a TrimScope Matrix II from Miltenyi Biotec using an Olympus 25X W/NA 1.05 objective at a wavelength of 850 nm owing to Spectra Physics Insight X3+ laser.
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bioRxiv - Bioengineering 2024Quote: ... at a ratio of 1:2 in NK MACS media (Miltenyi Biotec, Gaithersburg, MD) supplemented with 5% human AB serum (Millipore Sigma ...
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bioRxiv - Immunology 2024Quote: ... 5% human AB serum (Biowest) and 40 IU/ml of IL-2 (Miltenyi Biotec). 24 hours later ...
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bioRxiv - Microbiology 2024Quote: Splenocytes were incubated with SARS-CoV-2 S peptide pools (1μg/ml, Miltenyi Biotec) for 6 h in the presence of GolgiPlug (BD Bioscience) ...
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bioRxiv - Immunology 2019Quote: CD4+ and CD14+ cells were enriched from the PBMCs of 3 healthy donors via magnetic separation using the corresponding MicroBeads (Miltenyi Biotec). The CD14+ fraction was cryopreserved in liquid nitrogen ...
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bioRxiv - Cell Biology 2020Quote: Pre-conditioned media from FAP cell culture stimulated 3 days with PDGF-AA (20 ng/ml, human PDGF-AA, Cat nb. 130-108-983, Miltenyi Biotec) or control was collected and stored at −20°C ...
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bioRxiv - Immunology 2022Quote: ... supplemented with cytokines (IL-6 10ng/ml, IL-3 20ng/ml, M-CSF 50ng/ml and SCF 20ng/ml; all Miltenyi Biotec), with 2.5% Pen/Strep and 20μM L-Glutamine (Sigma-Aldrich) ...
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bioRxiv - Genetics 2019Quote: ... The flow-through was pelleted at 1250 rpm in a swinging bucket centrifuge set to 6°C then resuspended in 3 ml cold AutoMACs Running Buffer (AMB, Miltenyi Biotec). After gentle resuspension ...
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bioRxiv - Developmental Biology 2020Quote: ... Cell clumps and debris were removed by two consecutive rounds of centrifugation 3 minutes at 200xg and filtration through 30 µm cell strainers (Miltenyi Biotec). Additional mechanical dissociation and removal of cell aggregates was achieved by gentle pipetting up and down 5 times using a P200 pipette and Gel Saver tips (QSP) ...
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bioRxiv - Immunology 2020Quote: ... after which 3 mL of FACS buffer was added and the mixture was passed over a magnetized LS column (Miltenyi Biotec). The column was washed once with 5 mL ice-cold FACS buffer and then removed from the magnetic field and 5 mL ice-cold FACS buffer was pushed through the unmagnetized column twice using a plunger to elute the bound cell fraction.
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bioRxiv - Immunology 2021Quote: ... 21 and 63 DPI samples (n=3) were depleted of alveolar macrophages using a MACS cell separation LS column (Miltenyi Biotec) after staining for CD14 and CD172a (see Table S2) ...
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bioRxiv - Genomics 2021Quote: ... cells were blocked with 3%BSA diluted in PBS for 1h at RT and then incubated with anti-CD-19-PE antibody (1:50) in 3% BSA-PBS (Miltenyi Biotec) for 1h at RT ...
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bioRxiv - Developmental Biology 2022Quote: The soft palate from E13.5 embryos was dissected and digested using Multi Tissue Dissociate Kit 3 (Miltenyi Biotec, 130-110-204) at 37°C (600 rpm for 15 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... Remaining clumps of cells and debris were removed via two rounds of centrifugation for 3 min at 200 g followed by filtration through 30 μm cell strainers (Miltenyi Biotec). To ensure all remaining cells aggregates were dissociated ...
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bioRxiv - Biophysics 2022Quote: ... Naive T cells were purified via magnetic negative selection from spleens of 6–8 week old mice using either CD4+ (for 3.L2) or CD8+ (for P14 and OT1) T-cell isolation kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... JAM-1 and CD45 (Fig. 3, Supplementary Table 4) for 15 min prior to analysis on a MACSQuant10 Flow Analyzer (Miltenyi Biotec). To determine proportions of SCs and FAPs (Fig ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 to 7 Nematostella polyps or 3 Aiptasia polyps constituted one biological sample and were transferred to tissue homogenization tubes (“C-tubes”, 130-096-334, Miltenyi Biotec) after they were relaxed ...
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bioRxiv - Cancer Biology 2022Quote: ... standard CAR-T cells for the clinical setting were produced except for several modifications(20): CD8+ T cells were stimulated with 1 ug/ml OKT-3/CD28 antibodies (Miltenyi Biotec) for 72 h ...
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bioRxiv - Pathology 2019Quote: ... and blocked in FACS buffer (PBS, 2% BSA) containing an FcR blocking reagent (Miltenyi Biotec). Samples were stained with fluorophore-conjugated antibody cocktails ...
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bioRxiv - Developmental Biology 2020Quote: ... Samples resuspended in 2% FBS/PBS solution were blocked with mouse Fc Block (Miltenyi Biotec) for 5 minutes on ice ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were washed twice with 1× PBS + 2% FBS and fed to autoMACS (Miltenyi Biotec) to enrich for CD117+ cells ...
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bioRxiv - Neuroscience 2022Quote: ... Astrocyte fraction was enriched using Anti-ACSA-2 MicroBead Kit (Miltenyi Biotec, #130-097-679).
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bioRxiv - Neuroscience 2021Quote: ... cell pellets were resuspended in 1 mL FACS buffer (DPBS with 2% BSA, Miltenyi Biotec #130-091-376 ...
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bioRxiv - Immunology 2020Quote: ... anti-Dectin-2 PE (REA1001) and anti-CD64 APC (REA286) were purchased from Miltenyi Biotec; rat anti-Dectin-2 (D2.11E4 ...
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bioRxiv - Neuroscience 2022Quote: ... followed by addition of 150 μL Anti-ACSA-2 microbeads (Miltenyi Biotec 130-097-678). The sample was placed on a rotating wheel at 4°C for 30 min ...
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bioRxiv - Immunology 2023Quote: ... and the membrane glycoprotein (PepTivator® SARS-CoV-2 Prot_M) were obtained from Miltenyi Biotec. The PepTivator® Peptide Pools are constituted by peptides of 15 amino acid length with 11 amino acid overlap ...
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bioRxiv - Neuroscience 2023Quote: ... 2) 4.17 mM GadoSpin-P (n=6; MW=200 kDa; Miltenyi Biotec, Bergisch Gladbach, Germany); and 3 ...
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bioRxiv - Neuroscience 2023Quote: ... pre-filled with 2 mL ice-cold Nuclei Extraction Buffer (#130-128-024, Miltenyi Biotec) supplemented with 0.2 U/μL RNase inhibitor (#2489605 ...
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bioRxiv - Immunology 2024Quote: ... CD8- thymocytes were obtained by negative selection using anti-CD8 (Ly-2) MicroBeads (Miltenyi Biotec). The cells were then stained with fluorescently labeled antibodies to CD4 ...
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bioRxiv - Immunology 2024Quote: ... cells were stimulated with either PepTivator® SARS-CoV-2 Prot_S-peptide pool (Miltenyi Biotec) or S recombinant protein (Sino Biological ...
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bioRxiv - Immunology 2024Quote: Tumors were dissociated using Multi Tissue Dissociation Kit 2 (Miltenyi Biotec, Cat#: 130-110-203) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... We stimulated splenocytes and lung cells (3 × 105) in vitro with either N-protein peptide pool (Miltenyi Biotec; #130-126-699, Germany) or S-protein peptide pool (Genscript ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were spun at 400 g for 3 minutes at 4°C and washed in MACS Buffer (Miltenyi Biotec, 130-091-222) supplemented with 4% FBS (FBS ...
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bioRxiv - Neuroscience 2020Quote: ... ~100 μg Fe / 1 × 106 cells (40 μl Miltenyi Biotec FeraTrack SPIO / 2 ml culture medium) was chosen according to cell viability and manufacturer’s instructions ...
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bioRxiv - Biophysics 2019Quote: ... 5% CO2 in the presence of IL-2 (50⍰ng/ml; Miltenyi Biotec, Bergisch Gladbach, Germany) and used 6 to 10⍰days after stimulation ...
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bioRxiv - Immunology 2021Quote: ... M and N protein pools [1 µg/mL] (PepTivator® SARS-CoV-2 regents; Miltenyi Biotec) in 96-well U-bottom plates at 0,5-1×106 PBMCs per well ...
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bioRxiv - Neuroscience 2020Quote: Astrocyte and microglia were purified by anti-ACSA-2 micro beads (130-097-679, Miltenyi Biotec) and anti-CD11b micro beads (130-049-601 ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 40 ng/ml basic fibroblast growth factor-2 (FGF2) (Miltenyi Biotec, Bergisch Gladbach, Germany), 20 ng/ml epidermal growth factor (EGF ...
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bioRxiv - Neuroscience 2022Quote: ... Microglia and astrocytes were respectively isolated using Anti-CD11b and Anti-ACSA-2 MicroBeads (Miltenyi Biotec). Cells were counted and resuspended in 10-15 µL and 20-30 µL of buffer (0.05% BSA in DPBS ...
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bioRxiv - Biophysics 2019Quote: ... 5% CO2 in the presence of IL-2 (50 ng/ml; Miltenyi Biotec, Bergisch Gladbach, Germany) and used 7 days after activation ...
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bioRxiv - Synthetic Biology 2020Quote: ... and the media was further supplemented with 100 U/ml human IL-2 IS (Miltenyi Biotec), 1 ng/ml IL-15 (Miltenyi Biotec) ...
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bioRxiv - Immunology 2022Quote: ... the PepTivator® SARS-CoV-2 Prot_S B.1.1.529 Mutation Pool (130-129-928, Miltenyi Biotec) and SARS-CoV-2 Spike Glycoprotein B.1.1.529-Omicron (RP30121 ...
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bioRxiv - Immunology 2022Quote: ... PepTivator® SARS-CoV-2 Prot_S B.1.1.529 WT Reference Pool (130-129-927, Miltenyi Biotec) and SARS-CoV-2 Spike Glycoprotein-crud (RP30020 ...
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bioRxiv - Bioengineering 2023Quote: ... Conjugated primary antibodies used was anti-cardiac troponin 2 (TNNT2) PE (Miltenyi Biotec – 130-120-405). Viability marker Zombie Aqua (Biolegend – 423102 ...