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Citations for Miltenyi Biotec :
301 - 350 of 425 citations for 7 Oxabicyclo 2.2.1 hept 5 ene 2 carboxylicacid 2 hydroxy 1R 2R 4R rel 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... Extracted protein samples were centrifuged at 20 000g for 2 min at 4°C and the supernatant was collected for immunoprecipitation using the µMACS GFP Isolation Kit (Miltenyi Biotec, MediRay, New Zealand). Total and immunoprecipitated proteins were resolved on a 4–12% SDS-PAGE gel ...
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bioRxiv - Developmental Biology 2023Quote: ... the dorsolateral neocortex was dissected and a single cell suspension was produced from 2-3 hemispheres per condition using the MACS Neural Tissue Dissociation kit containing papain (Miltenyi Biotec, 130-092-628). Cells were fixed in 1% formaldehyde for exactly 10 min on a rotating wheel (10 rpm ...
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bioRxiv - Microbiology 2023Quote: ... Cells were allowed to recover for 30 min to 2 h at 37C and were then brought to 2.5e6 cells/mL in RPMI complete media with 100 U/mL IL-2 and at a 1:1 ratio with activation beads (Miltenyi Biotec #130-091-441). Two days later ...
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bioRxiv - Developmental Biology 2023Quote: ... Cerebral cortex was minced to small 2-3 mm pieces and dissociated using Neural Tissue Dissociation Kit P compounds (Miltenyi Biotec, 130-092-628) to obtain a cell suspension ...
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bioRxiv - Cancer Biology 2023Quote: Tumors from orthotopic PDAC mice were minced into 2-4 mm pieces and added with an enzyme mix from a tumor dissociation kit (Miltenyi Biotec, Bergisch Gladbach, Germany) in Roswell Park Memorial Institute medium 1640 (Cytiva ...
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bioRxiv - Cancer Biology 2023Quote: ... mixture at room temperature for 30 minutes and subsequently adding into 10 mL of RPMI+10%FBS and 2 uL of IL4 (Miltenyi Biotec 130-106-196). Cells were seeded at 0.5 × 106 per mL of CD40 stimulation medium at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... the cells were washed with PBS and resuspended in 50 µl of 1X annexin binding buffer containing 2 µl of FITC-annexin V (Miltenyi Biotec, 130-092-052). Cells were then incubated at room temperature for 30 min in the dark ...
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bioRxiv - Cancer Biology 2024Quote: ... frozen single cell suspensions from MDOTS processing (see above) were thawed and CD8+ TILs were isolated using Cd8a (LY-2) Microbeads (Miltenyi Biotec, 130-117-044). T cells were cultured in RPMI (Corning ...
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bioRxiv - Immunology 2020Quote: ... iMS1 and iMono cells were isolated after 7 d of culture using human CD14 Microbeads (Miltenyi Biotec). Isolated CD14+ cells were validated using flow cytometry (CD14-FITC ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... Cells were centrifuged and resuspended with fresh media with 40 μL/mL of PeptTivator® SARS-CoV-2 Prot_S (Miltenyi Biotec, Cat# 130-126-700), 1 μL/mL monensin (BioLegend) ...
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bioRxiv - Immunology 2021Quote: ... and the respective SARS-CoV-2 peptide pool (0.6 nmol/peptide, S and N pools from Miltenyi Biotec, 130-126-701 and 130-126-699). Unstimulated samples were used for subtraction of background cytokine production ...
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bioRxiv - Immunology 2020Quote: Pools of lyophilized peptides covering the immunodominant sequence of the spike glycoprotein and the complete sequence of the membrane glycoprotein of SARS-CoV-2 (15-mer sequences with 11 amino acids overlap) were obtained from Miltenyi Biotec (Thieme et al., 2020) resuspended and stored according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2022Quote: ... The lungs were incubated for 20min at 37 ◦C under continuous rotation and then run on the gentleMACS m-Lung-2 program on the gentleMACS (Miltenyi Biotec, Cat #130-095-937). This was followed by brief centrifugation and the passage of cells through the 70-uM filter ...
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bioRxiv - Developmental Biology 2023Quote: ... The lungs were incubated for 20min at 37 ◦C under continuous rotation and then run on the gentleMACS m-Lung-2 program on the gentleMACS (Miltenyi Biotec, Cat #130-095-937). This was followed by brief centrifugation and the passage of cells through the 70-uM filter ...
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bioRxiv - Immunology 2024Quote: ... AVX/COVID-12 or 30 nM of a mixture of 10-15 amino acid long peptides derived from the SARS-CoV-2 spike protein (PepTivator, Miltenyi Biotec, North Rhine-Westphalia, Germany). In parallel ...
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bioRxiv - Immunology 2020Quote: ... IL-5-Vio515 (Miltenyi Biotec), IL-4-PE Dazzle594 and IL-13-BV421 (Biolegend) ...
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bioRxiv - Genomics 2023Quote: ... 2 mM EDTA; dilution of MACS BSA Stock Solution [Miltenyi Biotec, 130-091-376] 1:20 with autoMACS® Rinsing Solution [Miltenyi Biotec, 130-091-222]).
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bioRxiv - Immunology 2021Quote: ... The isolated T cells were then stimulated with IL-7 and IL-15 (Miltenyi Biotec, Bergisch Gladbach, Germany) at a concentration of 25μg/2L bag of TexMACS media with 3% human AB serum (Innovative Research ...
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bioRxiv - Neuroscience 2020Quote: ... 10-20 cortices of PN day 2-4 pups were dissociated using the MACS Neural Tissue Dissociation Kit-T (Miltenyi Biotec, Cat# 130-093-231, Auburn, CA) at 37°C (5% CO2 ...
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bioRxiv - Immunology 2020Quote: C1 and C4-plasma generated cells were isolated after 7 d of culture using human CD14 Microbeads (Miltenyi Biotec). Isolated CD14+ cells were validated using flow cytometry (CD14-FITC ...
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bioRxiv - Molecular Biology 2022Quote: ... Plated neurospheres were maintained for 7 days on N2B27 basal medium supplemented with Dual Smad inhibitors (Miltenyi Biotec (SB), Tocris (DM)) ...
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bioRxiv - Genomics 2023Quote: ... TB6505 and TB6545 (1 givinostat and 7 vehicle slices) were dissociated using the Adult Brain Dissociation kit (Miltenyi Biotec) with modifications ...
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bioRxiv - Genetics 2023Quote: ... 1 × 10∧7 cells were processed for dead cell depletion using Dead Cell Removal Kit (Miltenyi Biotec # 130-090-101) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... and 5 ng/mL EGF (Miltenyi Biotec). Half of the medium was changed every day until the first colonies appeared ...
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bioRxiv - Immunology 2022Quote: ... antibodies in T cell media (RPMI containing 10% FBS, 2mM L-Glutamine, 1% Penicilin-Streptomycin) with IL-15 and IL-7 (Miltenyi Biotec, 10ng/ml each ...
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bioRxiv - Cancer Biology 2023Quote: ... and an enzymatic cocktail (supplementary table 7) and digested at 37°C for 42 minutes (37C_m_TDK_2 program) on a gentleMACS Octo Dissociator (Miltenyi Biotec, 130-096-427). Cell suspensions were then filtered through a 40 μm cell strainer (Corning ...
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bioRxiv - Immunology 2023Quote: ... 1×106 vehicle or ACCi-conditioned OT-1 T cells were infused into B16-OVA tumor bearing mice 10 days after tumor challenge and tumors were harvested 7 days later using the Mouse Tumor Dissociation Kit and gentleMACS Octo dissociator with heaters (Miltenyi Biotec) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2022Quote: TILs were stimulated for 7 days with anti-biotin MACSiBead Particles loaded with biotinylated CD3/CD28 antibodies (Miltenyi Biotec, Bergisch Gladbach, Germany). The preparation of the conjugated beads was carried out according to the manufacture’s recommendation (activation/expansion kit ...
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bioRxiv - Immunology 2022Quote: ... and 5 ng/ml human FLT3L (Miltenyi Biotec) as described previously 50.
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bioRxiv - Molecular Biology 2019Quote: ... FGF2 (5 ng/mL, Cat # 130093837, Miltenyi Biotec), insulin (same as above ...
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bioRxiv - Cancer Biology 2019Quote: ... CD44 Vioblue (REA690, Miltenyi Biotec, 1:5 dilution) used in Figure 1f
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bioRxiv - Cell Biology 2023Quote: ... and 5 ng/mL GM-CSF (Miltenyi Biotec) at 37 °C in a humidified atmosphere with 5% CO2.
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bioRxiv - Immunology 2023Quote: ... human TGFβ-1 (5 ng/ml, Miltenyi Biotec), anti-mouse IL-4 (11B11 ...
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bioRxiv - Cancer Biology 2019Quote: ... and FcR blocking reagent (Miltenyi Biotec, dilution 1:5) or isotype control antibody (mouse IgG1 ...
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bioRxiv - Developmental Biology 2022Quote: ... The cells are centrifugated at 600 g for 7 min at 4 °C and resuspended with 180 μl running buffer (Miltenyi Biotec, 130-091-221) for MACS selection.
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bioRxiv - Bioengineering 2022Quote: ... and then the cell pellet was resuspended in 100 µL of MACS buffer and 25 µL of anti-integrin α-7 microbeads (Miltenyi Biotec, #130-104-261) for incubation at 4 °C for 15 minutes ...
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bioRxiv - Immunology 2023Quote: ... Media was replenished every 48 hours for 7 days at which point BMDCs were harvested and magnetically selected using CD11c specific microbeads (Miltenyi Biotec, Cat# 130-125-835).
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bioRxiv - Microbiology 2020Quote: ... 5 million units per kg recombinant mouse IFNα (Miltenyi Biotec) were injected intravenously ...
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bioRxiv - Immunology 2023Quote: ... IL-1β (5 ng/m, #130-093-898 Miltenyi Biotec) and PGE2 (10 ug/ml ...
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bioRxiv - Cancer Biology 2019Quote: ... Naïve mouse B-cells were isolated from the spleen of 7–10 weeks old wild type or c-mycf/f mice with the B-cell isolation kit (MACS Miltenyi Biotec Cat. no. 130-090-862). After purification ...
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bioRxiv - Microbiology 2023Quote: ... Murine neutrophils were isolated from bone marrow of 7–12-week-old mice using an Anti-Ly-6G MicroBeads kit (Miltenyi Biotec; Cat. No. 130-120-337) per the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... or normal tissue were placed in 50 ml Falcon tubes containing 7-10 ml of MACS® Tissue Storage Solution (Miltenyi Biotec, cat. 130-100-008) and stored at room temperature for no longer than 2 hours ...
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bioRxiv - Immunology 2019Quote: ... 5% CO2.in the presence of 1μg/ml CD40 (Miltenyi Biotec). Activated BKV-specific T-cells were MACS isolated using the IFNγ Secretion Assay – Cell Enrichment and Detection Kit ...
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bioRxiv - Molecular Biology 2019Quote: ... 5 μg/L FGF2 (Cat # 130093838, Miltenyi Biotec, San Diego, CA), 5 mg/L insulin (Cat # SC360248 ...
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bioRxiv - Neuroscience 2020Quote: ... for 5 minutes and Anti-Biotin MicroBeads (Miltenyi Biotec, 130-090-485) for 10 minutes at 2-8°C ...
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bioRxiv - Genomics 2021Quote: Libraries were prepared from 5 fmol miRXplore Universal Reference Pool (Miltenyi Biotec) and 200 ng total cellular RNA using fixed NEBNext-adapters or 5N-adapters ...
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bioRxiv - Neuroscience 2022Quote: ... resuspended in the presence of anti-biotin MicroBeads (1:5; Miltenyi Biotec) and incubated for 15 min at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... we added an ice-cold buffer of 5% autoMACS Rinsing Solution (Miltenyi Biotec) in 1X MACS BSA solution (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2022Quote: ... 5×105 mononuclear cells were blocked with human FcR blocking reagent (Miltenyi Biotec) for 5 min and stained in FACS buffer (Phosphate buffer saline - PBS plus 2 mM EDTA ...
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bioRxiv - Cancer Biology 2023Quote: ... at 4°C and distributed at 5 mL per LS column (Miltenyi Biotec), or 5×106 BM MNC were thawed ...