Labshake search
Citations for Miltenyi Biotec :
101 - 150 of 809 citations for 7 Hydroxy 2 3 dihydro 1H cyclopenta c chromen 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: ... CD4+ T cells were incubated for 72 hours at 37°C with either anti-CD3/anti-CD28 beads (1:2 bead-to-cell ratio, Miltenyi Biotec) or 200ng/mL N-803 in the presence of 50µM raltegravir and 5µM saquinavir ...
-
bioRxiv - Immunology 2023Quote: ... Cells (5×106) were infected at 5000 TCID50 / 1×106 cells at 37 °C for 2 hours on a cell-rotator (Miltenyi Biotec). After infection ...
-
bioRxiv - Immunology 2023Quote: ... splenocytes were resuspended in CTL test media (Immunospot) and incubated at 37 °C for 2 hours with either no stimulation or S1 peptide simulation (Miltenyi Biotec) at a final concentration of 2 µg/mL before adding GolgiPlug to all samples for 4 additional hours ...
-
bioRxiv - Immunology 2023Quote: ... and incubated at 37°C for total of 30 minutes (15 minutes, 2 times) with rotated using MACSmix Tube Rotator (Miltenyi Biotec). Between and after the two incubations ...
-
bioRxiv - Molecular Biology 2023Quote: ... 0.4 x 106 isolated T cells from the same donor and 0.4 x 106 T cell activation beads (Miltenyi Biotec, anti-CD2/3/28) were added ...
-
bioRxiv - Plant Biology 2019Quote: ... Supernatants were adjusted to 5 mg/mL protein concentration and incubated for 1 h at 4 °C with 100 μL of anti-GFP magnetic beads (Miltenyi Biotec, #130-091-125). Bound proteins were analyzed by immunoblotting with anti-GFP (Invitrogen ...
-
bioRxiv - Microbiology 2020Quote: ... to analyze pDCs for 20 min at 4°C to analyze B cells or with two antibodies directed against CD303(BDCA2)-APC (Miltenyi Biotec, #130-097-931) and CD304(BDCA4)-PE (Miltenyi Biotec # 130-112-045) ...
-
bioRxiv - Immunology 2019Quote: ... centrifuged at 500g for 10 min at 4 °C and resuspended in 0XTD supplemented with 100 ng/ml each of Stem Cell Factor (SCF, Miltenyi Biotec, 130-096-695), Flt3 Ligand (Flt3L ...
-
bioRxiv - Immunology 2022Quote: ... After centrifugation (5 min, 500 x g, 4 °C) supernatant was discarded and cells were resuspended in MACS buffer (Miltenyi Biotec, #130-091-221) for following cell sorting.
-
bioRxiv - Cancer Biology 2023Quote: ... For CD8+ T cells magnetic isolation we incubated the cell suspension 15 min incubation at 4°C with CD8 microbeads (Miltenyi Biotec, #130-045-201), then cells were passed through LS magnetic columns (Miltenyi Biotec ...
-
bioRxiv - Cancer Biology 2020Quote: ... and subsequently stained at 4°C with antibodies against following human antigens: CD3 (REA613, APC-Vio770, Miltenyi Biotec, Cat no.130-113-698, 1:50), CD4 (A161A1 ...
-
bioRxiv - Immunology 2022Quote: ... Cells were labeled for at least 20 minutes at 4 ° C and CD14+ cells were isolated by magnetic column (Miltenyi Biotec, catalog# 130-042-201).
-
bioRxiv - Microbiology 2024Quote: ... or 30 ng/mL interleukin-4 (IL-4; Miltenyi Biotec) (M2 ...
-
bioRxiv - Cancer Biology 2021Quote: Surgical specimens placed immediately in cold sterile DMEM after excision were cut into 2-4 mm pieces before undergoing enzymatic and mechanical dissociation using the gentleMACS™ Octo Dissociator multitissue kit and protocol (Miltenyi Biotec). Resuspended cells were then promptly plated into flasks ...
-
bioRxiv - Bioengineering 2023Quote: ... [4] (Miltenyi Biotec), and the lineage-negative NSG HSPCs were collected after MACS depletion of the lineage-positive marrow cells ...
-
bioRxiv - Immunology 2021Quote: ... and the cytokines IL-7 and IL-15 (Miltenyi Biotec, Bergisch Gladbach, Germany). Peripheral blood apheresis products were loaded into the machine and CD4 and CD8 T cells were isolated using CliniMACS CD4 reagent and CliniMACS CD8 reagent according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... was supplemented with 10 ng/ml IL-7 (#130-093-937, Miltenyi Biotec) and 3 ng/ml IL-15 (#130-093-955 ...
-
bioRxiv - Immunology 2024Quote: ... supplemented with 10 ng/ml of IL-7 and IL-15 (Miltenyi Biotec) and 1% P/S (Biowest ...
-
bioRxiv - Bioengineering 2024Quote: ... 20 µL of anti-integrin α-7 microbeads (Miltenyi Biotec, #130-104-261) was added to the cell suspension and then incubated at 4 °C for 15 min ...
-
bioRxiv - Developmental Biology 2023Quote: ... the dorsolateral neocortex was dissected and a single cell suspension was produced from 2-3 hemispheres per condition using the MACS Neural Tissue Dissociation kit containing papain (Miltenyi Biotec, 130-092-628). Cells were fixed in 1% formaldehyde for exactly 10 min on a rotating wheel (10 rpm ...
-
bioRxiv - Developmental Biology 2023Quote: ... Cerebral cortex was minced to small 2-3 mm pieces and dissociated using Neural Tissue Dissociation Kit P compounds (Miltenyi Biotec, 130-092-628) to obtain a cell suspension ...
-
bioRxiv - Cancer Biology 2022Quote: ... samples were cut into approximately 2 – 4mm chunks with forceps and a scalpel and transferred to a gentleMACS C tube (Miltenyi Biotec, 130-093-237) containing 4.7 ml RPMI 1640 base media ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... CD4 (VIT-4, PerCp, Miltenyi Biotec, VIT-4, VioBlue, Miltenyi Biotec), CD8 (RPA-T8 ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... CD4 (VIT-4, PerCp, Miltenyi Biotec, VIT-4, VioBlue, Miltenyi Biotec), CD8 (RPA-T8 ...
-
bioRxiv - Developmental Biology 2022Quote: ... 3 μM GSK3i (Miltenyi Biotec) and 10 μM of ROCKi (Y-27632 ...
-
bioRxiv - Immunology 2020Quote: ... Nylon wool-enriched splenic T cells from naive or immunized mice were incubated sequentially for 10 min at 4°C with FITC-conjugated anti-mouse γδ TCR or αβ TCR Abs and 15 min at 4°C with anti-FITC Microbeads (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany), then the cells were separated into bound and non-bound fractions on an autoMACS™ separator column (Miltenyi Biotec GmbH) ...
-
bioRxiv - Neuroscience 2024Quote: ... The supernatant was coupled with Anti-tomm22 micro-Beats (30min at 4° C) to remove free mitochondria through Magnetic-assisted cell sorting (MACS) (Miltenyi Biotec, 130-096-946, 130-042-401). The mitochondria-depleted flow-through was centrifuged at 13,000 × g for 10 min at 4° C to pellet synaptosomes (100ul of PBS resuspension ...
-
bioRxiv - Cancer Biology 2021Quote: ... Tumor suspensions were stained with 7-AAD live/dead stain (Miltenyi Biotec, Woking, UK), FITC-labeled CD3e (clone-145-2C11 ...
-
bioRxiv - Microbiology 2020Quote: ... Cells were passaged every 5-7 days using Stem-MACS Passaging Solution (Miltenyi Biotec). Differentiation of SV20 into cardiomyocytes (iCMs ...
-
bioRxiv - Microbiology 2019Quote: ... using C tubes (Miltenyi Biotec) in ice cold Dulbecco’s PBS supplemented with 0.1% BSA ...
-
bioRxiv - Developmental Biology 2022Quote: ... The lungs were incubated for 20min at 37 ◦C under continuous rotation and then run on the gentleMACS m-Lung-2 program on the gentleMACS (Miltenyi Biotec, Cat #130-095-937). This was followed by brief centrifugation and the passage of cells through the 70-uM filter ...
-
bioRxiv - Developmental Biology 2023Quote: ... The lungs were incubated for 20min at 37 ◦C under continuous rotation and then run on the gentleMACS m-Lung-2 program on the gentleMACS (Miltenyi Biotec, Cat #130-095-937). This was followed by brief centrifugation and the passage of cells through the 70-uM filter ...
-
bioRxiv - Cancer Biology 2019Quote: ... tumor samples were cut into pieces of 2-4 mm and suspended using the tumor dissociation protocol with the GentleMacs® dissociator (Miltenyi Biotec GmbH, Germany). The cell suspension was applied to a cell strainer (70 μm ...
-
bioRxiv - Cancer Biology 2023Quote: Tumors from orthotopic PDAC mice were minced into 2-4 mm pieces and added with an enzyme mix from a tumor dissociation kit (Miltenyi Biotec, Bergisch Gladbach, Germany) in Roswell Park Memorial Institute medium 1640 (Cytiva ...
-
bioRxiv - Pathology 2022Quote: ... and cells were stained for 30 min at 4°C with the following anti-human antibodies: PerCP-Vio700-conjugated REAfinity recombinant anti-CD31 (Miltenyi Biotec, clone REA730, #130-110-673, 1/20), BV421-conjugated anti-Tissue Factor/CD142 (BD Biosciences ...
-
bioRxiv - Microbiology 2020Quote: ... antibody for 45 min at 4°C followed by the incubation with the secondary antibody anti-IgG1-PE (REA1017, Miltenyi Biotec, 1 μL/105 cells in 100 μL) for 30 min at 4°C ...
-
bioRxiv - Pathology 2022Quote: ... Hearts were digested for 30 min at 37°C in gentleMACS C Tubes (Miltenyi Biotec) with 800 U/ml Collagenase II and 100 U/ml DNase I (Worthington Biochemical Corporation) ...
-
bioRxiv - Microbiology 2021Quote: ... purified mAbs were biotin-labelled using One-Step Antibody Biotinylation Kit (Miltenyi Biotec) following the manufacturer’s recommendations and purified using 40K MWCO Desalting Column (ThermoFisher Scientific) ...
-
bioRxiv - Microbiology 2020Quote: ... purified mAbs were biotin-labeled using One-Step Antibody Biotinylation Kit (Miltenyi Biotec) following manufacturer recommendations and purified using 40K MWCO Desalting Column (ThermoFisher Scientific) ...
-
bioRxiv - Developmental Biology 2020Quote: ... and 3 μM CHIR99021 (Miltenyi Biotec), together abbreviated as 2i ...
-
bioRxiv - Neuroscience 2019Quote: ... NT-3 (10ng/mL, Miltenyi Biotec), BDNF (10ng/mL ...
-
bioRxiv - Neuroscience 2022Quote: ... and 3 μM CHIR99021 (Miltenyi Biotec) were added to the medium ...
-
bioRxiv - Developmental Biology 2022Quote: ... and 3 μM CHIR99021 (Miltenyi Biotec). Cells were maintained at 37°C with 6% CO2.
-
bioRxiv - Cell Biology 2020Quote: ... CHIR99021 (CH, 3 μM, Miltenyi Biotec) and leukemia inhibitory factor (LIF ...
-
bioRxiv - Cell Biology 2020Quote: ... 3 μM CHIR 99021 (Miltenyi Biotec) and 150 μM LAAP (Merck) ...
-
bioRxiv - Genetics 2023Quote: ... and then 7 mL PEB buffer (1 volume of MACS BSA Stock Solution, Miltenyi Biotec, 130-091-376 ;19 volumes of autoMACS Rinsing Solution ...
-
bioRxiv - Pathology 2023Quote: ... and homogenized at 37°C in MACS C-tubes using the gentle MACS dissociator (Miltenyi Biotec), digestion neutralized with PEB buffer (2 mM EDTA ...
-
bioRxiv - Cancer Biology 2023Quote: ... Pre-enrichment for c-kit+ cells was achieved using anti-c-kit magnetic beads (Miltenyi Biotec).
-
bioRxiv - Immunology 2020Quote: ... and in C Tubes (Miltenyi Biotec) disassociated using the gentleMACS Octo Dissociator (Miltenyi Biotec) ...
-
bioRxiv - Microbiology 2020Quote: ... using a C-Tube (Miltenyi Biotec), which allows dissociation of viable single cells from tissue samples ...