Labshake search
Citations for Miltenyi Biotec :
51 - 100 of 761 citations for 7 CHLORO 10 11 DIHYDRO 5H DIBENZ B F ACEPIN 2 OL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2020Quote: ... primary B cells from PBMCs using B Cell Isolation Kit II (Miltenyi Biotec, >90% purity by anti-CD19 staining). PBMCs were rested and stimulated with 100 ng/ml LPS (from E ...
-
bioRxiv - Immunology 2022Quote: ... 5 ng/ml human IL-7 (Miltenyi Biotec), and 5 ng/ml human FLT3L (Miltenyi Biotec ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... 25 U/mL human IL-7 (Miltenyi Biotec) and 50 U/mL human IL-15 (Miltenyi Biotec ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... 25 U/mL human IL-7 (Miltenyi Biotec) and 50 U/mL human IL-15 (Miltenyi Biotec) ...
-
bioRxiv - Immunology 2023Quote: ... with IL-7 and IL-15 (Miltenyi Biotec) added at 10ng/mL and 5ng/mL ...
-
bioRxiv - Pathology 2024Quote: ... Isotype controls or fluorescence minus one (FMO) controls were used, and the live/dead marker 7-aminoactinomycin D (7-AAD, PerCP) (Miltenyi Biotec) was added to all samples 10 min before analysis (10 μl to 1 ml of cell suspension).
-
bioRxiv - Immunology 2019Quote: ... Murine B cells were enriched by negative selection of IgMpos cells with a mouse B cell isolation kit (Miltenyi Biotec, #130-090-862 ...
-
bioRxiv - Immunology 2021Quote: ... naïve splenic CD43− B cells were magnetically isolated using the B cell isolation kit (Miltenyi Biotec, Inc.; 130-090-862) and LS columns (Miltenyi Biotec ...
-
bioRxiv - Immunology 2021Quote: ... Human naïve B cells were isolated using the MACSxpress Whole Blood B Cell Isolation Kit (Miltenyi Biotec, 130-098-190) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: Splenic and mLN naïve B cells were purified using negative B cell isolation kits yielding enriched populations of 95%–98% (Miltenyi Biotec). Splenic naïve CD4 T cells were purified using negative Naïve CD4+ T cell isolation Kit yielding a cell purity of 97%-99% (Miltenyi Biotec) ...
-
bioRxiv - Immunology 2022Quote: ... and CD19+CD43- non-B-1 cells were purified using Mouse B Cell Isolation Kit (130-090-862, Miltenyi Biotec) which contains anti-CD43 antibody ...
-
bioRxiv - Immunology 2023Quote: Memory B cells and naïve B cells were isolated from RosetteSep pre-sorted total B cells using MACS cell sorting from Miltenyi Biotec. Memory B cells were obtained via positive selection using the Milteny Memory B cell isolation kit ...
-
bioRxiv - Cancer Biology 2023Quote: ... and B-CLL cells were further purified using the Pan B-cell Isolation Kit (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany). Versalyse buffer (Beckman-Coulter ...
-
bioRxiv - Molecular Biology 2023Quote: ... CD43− B cells were enriched from the spleen by immunomagnetic depletion of non-B cells with CD43-MicroBeads (Miltenyi Biotec). The following monoclonal antibodies were used for flow-cytometric analysis of mouse lymphoid organs from 4-12 week-old mice ...
-
bioRxiv - Microbiology 2024Quote: ... consisting mainly of 15-mer sequences with 11-amino-acid (aa) overlap (Miltenyi Biotec). Negative controls for non-stimulated cells were included in the test ...
-
bioRxiv - Immunology 2022Quote: ... B cell kit (Miltenyi Biotec, 130-090-862) was followed according to the manufacturer’s protocol.
-
bioRxiv - Microbiology 2023Quote: ... Cells were stimulated with IL-2 (10 ng/ml) (Miltenyi Biotec #130-097-745) and with anti-CD3/CD28 beads (Gibco #11132D ...
-
bioRxiv - Immunology 2021Quote: ... naïve B cells were isolated from the spleens of mice using the B cell isolation kit (Miltenyi Biotec: 130-090-862) and 30×103 cultured on the irradiated 40LB cells with IL-4 in supplemented RPMI-1640 media (final ...
-
bioRxiv - Immunology 2020Quote: ... of cytapheresis rings from healthy donors (EFS Bordeaux) and B cells were sorted with the B cell isolation kit II (Miltenyi Biotec). Sorted B cells were cultured for 4 days in RPMI 1640 with UltraGlutamine (Lonza ...
-
bioRxiv - Immunology 2020Quote: Splenic B cells were isolated from 5 or 12 weeks old MRL/Lpr mice using B Cell Isolation Kit (Miltenyi Biotec) and stained with CSFE before co-culturing with purified TCRβ+CD138+ or TCRβ+CD138− cells in the presence of anti-CD3/CD28 antibodies (BD Biosciences) ...
-
bioRxiv - Immunology 2021Quote: B cells were isolated from spleens of WT female mice and enriched with a B cell isolation kit (Miltenyi Biotec MACS). The purity of isolated B cells was 96±0.8% ...
-
bioRxiv - Immunology 2021Quote: ... B cell isolation from cryopreserved PBMCs was carried out using Memory B cells Isolation Kit (MACS/Miltenyi Biotec, Bergisch Gladbach, Germany). Next ...
-
bioRxiv - Immunology 2021Quote: ... splenic B cells were enriched by magnetic microparticle purification using mouse B Cell Isolation Kit (Miltenyi Biotec, Cat#130-090-862) and then further sorted by gating CD11c+ B cells (CD19+CD11c+CD21- ...
-
bioRxiv - Immunology 2023Quote: PBMCs from three different donors were isolated from human buffy coat by Ficoll density gradient centrifugation and untouched B-cells were purified by negative magnetic enrichment using a B-cell isolation kit II (Miltenyi Biotec) according to the manufacturer’s instructions ...
-
bioRxiv - Biophysics 2023Quote: Resting B-cells were enriched from the spleen using the B-cell Isolation Kit (Miltenyi Biotec, Germany, Cat# 130-090-862). 1 mL 8 million/mL B-cells in HBSS buffer were sequentially incubated with 1 μM cholesterol strand (30 min ...
-
bioRxiv - Immunology 2023Quote: ... B cell enrichment of bone marrow cells was performed using the Pan B Cell Isolation Kit (Miltenyi Biotec, #130-095-813). MultiScreen-IP Filter Plates (Sigma ...
-
bioRxiv - Immunology 2023Quote: B220+ B cells were isolated via negative selection from single-cell suspensions by using the mouse B Cell Isolation Kit (Miltenyi Biotec), following the manufacturer’s instructions ...
-
bioRxiv - Immunology 2023Quote: A cluster of differentiation 19 (CD19)-positive B lymphocytes (total B cells) were purified from buffy coats derived from healthy donors using magnetic microbeads (Miltenyi Biotec, Bergisch Gladbach ...
-
bioRxiv - Immunology 2024Quote: ... supplemented with IL-7 and IL-15 (Miltenyi Biotec), while the adherent cells (monocytes/macrophages ...
-
bioRxiv - Microbiology 2022Quote: ... and stimulated with either SARS-CoV-2 S-trimer (10-100 µg/mL) or SARS-CoV-2 PepTivator® Peptide S and NP protein Pools (10 µg/mL each, Miltenyi Biotec) for 72 h at 37°C ...
-
bioRxiv - Microbiology 2021Quote: ... B-cells purified from donor PBMCs using the MACS Human B-Cells isolation kit (Miltenyi Biotec Miltenyi Biotec Cat# 130-091-151) were stained with a panel of antibodies ...
-
bioRxiv - Microbiology 2021Quote: ... B-cells purified from donor PBMCs using the MACS Human B-Cells isolation kit (Miltenyi Biotec Miltenyi Biotec Cat# 130-091-151) were stained with a panel of antibodies ...
-
bioRxiv - Microbiology 2021Quote: ... B lymphocyte isolation was conducted through negative selection by using the B cell Isolation Kit II (Miltenyi Biotec, Cat#130-091-151) according to the manufacturer’s protocol ...
-
bioRxiv - Genetics 2022Quote: ... or Pan B Cell (Miltenyi Biotec, 130-104-433) isolation kits ...
-
bioRxiv - Immunology 2023Quote: ... B cells were enriched with CD19 Microbeads (Miltenyi Biotec) or MojoSort Mouse Pan B cell Isolation Kit (BioLegend) ...
-
bioRxiv - Immunology 2023Quote: ... with Whole Blood B Cell Isolation Kit (Miltenyi Biotec). Sample acquisition was approved by the Regional Ethics Committee of South-Eastern Norway (project 6544) ...
-
bioRxiv - Immunology 2023Quote: ... or normal tissue were placed in 50 ml Falcon tubes containing 7-10 ml of MACS® Tissue Storage Solution (Miltenyi Biotec, cat. 130-100-008) and stored at room temperature for no longer than 2 hours ...
-
bioRxiv - Immunology 2021Quote: ... were isolated by gradient centrifugation and then enriched for B cells by negative selection using a pan-B-cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec, 130-101-638).
-
bioRxiv - Immunology 2021Quote: ... peripheral blood mononuclear cells were enriched for B cells by negative selection using a pan-B-cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were incubated in FACS buffer (1× PBS ...
-
bioRxiv - Immunology 2021Quote: ... peripheral blood mononuclear cells were enriched for B cells by negative selection using a pan-B-cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were incubated in Flourescence-Activated Cell-sorting (FACS ...
-
bioRxiv - Immunology 2022Quote: ... peripheral blood mononuclear cells were enriched for B cells by negative selection using a pan-B-cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were incubated in Flourescence-Activated Cell-sorting (FACS ...
-
bioRxiv - Immunology 2022Quote: ... peripheral blood mononuclear cells were enriched for B cells by negative selection using a pan-B-cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were incubated in FACS buffer (1× PBS ...
-
bioRxiv - Immunology 2022Quote: ... peripheral blood mononuclear cells were enriched for B cells by negative selection using a pan-B-cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were incubated in Flourescence-Activated Cell-sorting (FACS ...
-
bioRxiv - Immunology 2021Quote: ... peripheral blood mononuclear cells were enriched for B cells by negative selection using a pan-B-cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were incubated in FACS buffer (1× PBS ...
-
bioRxiv - Immunology 2021Quote: Murine naïve B cells were purified from spleen by magnetic negative selection by using Pan B Cell Isolation Kit (Miltenyi Biotec, 130-095-813) according to the manufacturer’s instructions and maintained in appropriate culture medium ...
-
bioRxiv - Immunology 2020Quote: PBMCs were enriched for B cells by negative selection using a pan B cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were incubated in FACS buffer (1 X Phosphate-buffered Saline (PBS) ...
-
bioRxiv - Immunology 2022Quote: ... were enriched for B cells by negative selection using a pan-B-cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were incubated in Flourescence-Activated Cell-sorting (FACS ...
-
bioRxiv - Bioengineering 2023Quote: ... and 7-AAD staining solution (130-111-568; Miltenyi Biotec) for dead cell exclusion on a MACS Quant 10 analyzer (Miltenyi Biotec) ...
-
bioRxiv - Cancer Biology 2023Quote: ... mixture at room temperature for 30 minutes and subsequently adding into 10 mL of RPMI+10%FBS and 2 uL of IL4 (Miltenyi Biotec 130-106-196). Cells were seeded at 0.5 × 106 per mL of CD40 stimulation medium at 37 °C ...
-
bioRxiv - Immunology 2022Quote: ... mouse B cells were first isolated from spleen and lymph nodes using the Pan B-cell Isolation Kit II (Miltenyi Biotec,130-104-443) following the manufacturer’s protocol ...