Labshake search
Citations for Miltenyi Biotec :
51 - 100 of 345 citations for 6 ethoxy 7H purin 2 amine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: ... and IL-2 (50 IU/mL; Miltenyi Biotec), and stimulated with Dynabeads (Gibco ...
-
bioRxiv - Immunology 2022Quote: ... IL-2-PE (Clone M7/48A, Miltenyi Biotec), CD14-PerCP (Clone TUK4 ...
-
bioRxiv - Cancer Biology 2023Quote: ... containing IL-2 (Miltenyi Biotec, # 130-097-748), human serum AB (Seralab ...
-
bioRxiv - Cell Biology 2023Quote: ... and 25 ng/mL IL-2 (Miltenyi Biotec). NCI-H1299 ...
-
bioRxiv - Bioengineering 2021Quote: ... and 5% Human AB serum (Gemini) supplemented with 50 units/mL IL-2 every 2 days for all experiments (Miltenyi Biotec).
-
bioRxiv - Microbiology 2021Quote: ... Splenocytes (0.25 × 106) were stimulated in duplicates with SARS-CoV-2 S- or N-peptide pool (2 µg/ml, Miltenyi Biotec, USA) for 24 hours at 37°C ...
-
bioRxiv - Immunology 2023Quote: ... purified CD8+ T-cells were coated with mouse IL-2 catch reagent from a mouse IL-2 secretion assay detection kit (Miltenyi Biotec) by incubating T cells in a 20x dilution of IL-2 catch reagent in R10 medium for 15 min on ice ...
-
bioRxiv - Neuroscience 2023Quote: ... and then co-stained for 15 min at 4 °C with PE-conjugated anti-astrocyte cell surface antigen-2 (ACSA-2) antibody (130-116-244; Miltenyi Biotec) and Alexa Fluor 647-conjugated anti-O1 antibody (FAB1327R ...
-
bioRxiv - Bioengineering 2023Quote: ... adjusted at 5×105 cells/ml and cultured 2-5 additional days in cell culture medium supplemented with 20 ng/ml IL-2 (Miltenyi Biotec) before being used in the experiments.
-
bioRxiv - Molecular Biology 2024Quote: ... program and cultured in TexMACS supplemented with 3% human serum (HS) (Life Science Production) and interleukin-2 (IL-2) (Miltenyi Biotec) for 24 hours ...
-
bioRxiv - Immunology 2022Quote: ... CD11c+ cells were sorted from pooled spleens of WT C57BL/6 mice by using mouse CD11c MicroBeads (Miltenyi Biotec). Then ...
-
bioRxiv - Immunology 2020Quote: Neutrophils from bone marrow were purified from C57Bl/6 mice by negative selection using neutrophil isolation kit (Miltenyi Biotec). Purified neutrophils were cultured at 1.0-5.0 x 107 total cells and were infected or not at different ratios with RFP LmRyn metacyclic promastigotes ...
-
bioRxiv - Immunology 2020Quote: ... cells were isolated from WT C57BL/6 spleens using a mouse naïve CD4+ T cell isolation kit (Miltenyi Biotec) and cultured as described previously (Shakya et al. ...
-
bioRxiv - Immunology 2021Quote: ... Cells were cultured for 18 h at 5×105 cells per well with 2 μg/ml of a peptide pool consisting mainly of 15-mers (overlapping by 11 amino acids) covering the immunodominant sequence domains of the SARS-CoV-2 Spike protein (PepTivator® SARS-CoV-2 Prot_S; Miltenyi Biotec, Germany). The number of spots was determined using an automatic ELISPOT reader and image analysis software (CTL-ImmunoSpot® S6 Micro Analyzer ...
-
bioRxiv - Immunology 2023Quote: ... CD4+ T cells were washed with 2% FACs buffer (PBS with 2% FBS) prior to resuspension in TexMACS complete media (130-097-196, Miltenyi Biotec, USA), supplemented with 10% FBS ...
-
Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: ... ACSA-2 clone IH3-18A3 in PE (Miltenyi Biotec); CD31 clone 390 in PerCP/Cy5.5 (BioLegend) ...
-
bioRxiv - Immunology 2022Quote: ... recombinant human IL-2 (100 IU/ml, Miltenyi Biotec), PHA (0,25 µg/ml ...
-
bioRxiv - Immunology 2020Quote: ... IL-2-PE (Miltenyi Biotec, cat #130-090-487) and TNF-α-APC (Miltenyi Biotec ...
-
bioRxiv - Immunology 2020Quote: ... and Prot_S (each at 2 μg/ml; Miltenyi Biotec). Negative control wells lacked peptides ...
-
bioRxiv - Molecular Biology 2023Quote: ... 2% NS-21 (MACS NeuroBrew-21 supplement (Miltenyi Biotec)) ...
-
bioRxiv - Molecular Biology 2023Quote: ... 2% NS-21 (MACS NeuroBrew-21 supplement (Miltenyi Biotec)) ...
-
bioRxiv - Molecular Biology 2023Quote: ... 2% NS-21 (MACS NeuroBrew-21 supplement (Miltenyi Biotec)) ...
-
bioRxiv - Physiology 2023Quote: ... 2% MACS® NeuroBrew-21 (Miltenyi Biotec, Surrey, UK), 2 μM PD0332991 (Tocris) ...
-
bioRxiv - Bioengineering 2024Quote: ... supplemented with 100 IU/mL IL-2 (Miltenyi Biotec)) ...
-
bioRxiv - Neuroscience 2024Quote: ... the ACSA-2 kit (#130-097-678, Miltenyi Biotec) and CD11b (#130-093-634 ...
-
bioRxiv - Neuroscience 2023Quote: ... ACSA-2 clone IH3-18A3 in PE (Miltenyi Biotec); CD31 clone 390 in PerCP/Cy5.5 (BioLegend) ...
-
bioRxiv - Molecular Biology 2023Quote: ... and 5 ng/ml human FGF-2 (Miltenyi Biotec). Then ...
-
bioRxiv - Bioengineering 2024Quote: ... 100 U/ml human IL-2 IS (Miltenyi Biotec) and 1 ng/ml IL-15 (Miltenyi Biotec ...
-
bioRxiv - Cell Biology 2020Quote: ... Naïve CD4+ T cells were isolated from the lymph nodes and spleen of C57BL/6 mice by MidiMACS (Miltenyi Biotec). Cells were activated by plate-bound anti-CD3 (5 μg mL−1 ...
-
bioRxiv - Cancer Biology 2022Quote: Primary NK cells were isolated and enriched the day of the experiment from the spleens of 8-12 week old female C57BL/6 mice using the NK Cell Isolation Kit II according to manufacturer’s instructions (Miltenyi Biotec). 50,000 NK cells were then seeded in the top chamber of a transwell insert (Corning ...
-
bioRxiv - Immunology 2022Quote: ... pan splenic T cells were isolated from crushed spleens of naïve C57BL/6 mouse using the Mouse Pan T Cell Isolation Kit (Miltenyi Biotec). In the subsequent co-culture incubation ...
-
bioRxiv - Immunology 2022Quote: ... cells were prepared from CD45.1 Thy1.2 C57Bl/6 mice by first crushing bones followed by T cell depletion using CD4 and CD8 MicroBeads (Miltenyi Biotec). CD45.2 Thy1.2 FoxP3/GFP+ CD4+ Treg were FACS sorted from CD8/CD19-depleted single cell suspensions from spleens and lymph nodes from CD45.2 Thy1.2 FoxP3GFP+ C57Bl/6 using a BD FACS Aria II ...
-
bioRxiv - Immunology 2022Quote: Splenic iNKT cells were isolated from C57BL/6 mice sorting CD45+CD3+CD1d:PBS57Tet+ cells upon enrichment through B cells exclusion (Mouse CD19 microbeads, Miltenyi Biotec). Bone marrow derived dendritic cells (BMDCs ...
-
bioRxiv - Immunology 2023Quote: Naïve CD4+ T cells were purified from spleen and lymph nodes of 6-15 week old 2D2 mice or OT-II mice using the naïve CD4+ T cell isolation Kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2022Quote: ... were processed and re-suspended in 80 µl MACS buffer (2% FBS, 2 mM EDTA in PBS) and 20 µl CD117 MicroBeads (MACS Miltenyi Biotec, Surrey, UK) to enrich LSK cells as per the manufacturers protocol ...
-
bioRxiv - Genomics 2020Quote: ... Stained cells were washed with cold PBS/10% FBS and resuspended in 8 μl of cold PBS/0.5% BSA/2 mM EDTA and 2 μl of anti-PE MACS microbeads (Miltenyi Biotec: 130-048-801) per 106 cells and incubated at 4 °C for 15 minutes ...
-
bioRxiv - Microbiology 2022Quote: ... 3 × 105 cells harvested from the spleen or cervical lymph nodes were incubated for 16 h in the presence of a peptide pool derived from the S protein of SARS-CoV-2 (a mixture of PepTivator SARS-CoV-2 Prot_S, S1, and S+; Miltenyi Biotec, Bergisch Gladbach, Germany). After washing the cells with PBS ...
-
bioRxiv - Molecular Biology 2022Quote: ... Stained cells were washed with cold PBS/10% FBS and resuspended in 8 μl of cold PBS/0.5% BSA/2 mM EDTA and 2 μl of anti-PE MACS microbeads (Miltenyi Biotec: 130-048-801) per 106 cells and incubated at 4 °C for 15 minutes ...
-
bioRxiv - Neuroscience 2020Quote: ... and Anti-ACSA-2 MicroBeads (Miltenyi Biotec, 130-097-678) for 10-15 minutes at 2-8°C ...
-
bioRxiv - Neuroscience 2021Quote: ... 2) Neuronal (Cd24-Vioblue (REA743, #130-110-831, Miltenyi Biotec)) ...
-
bioRxiv - Immunology 2022Quote: ... or 1 µg/mL SARS-CoV-2 Prot_S (Miltenyi Biotec), no antigen was added to control wells ...
-
bioRxiv - Cell Biology 2022Quote: ... supplemented with 100 U/ml human IL-2 (Miltenyi Biotec) and 100 U/ml Penicillin/Streptomycin (Gibco) ...
-
bioRxiv - Synthetic Biology 2023Quote: ... and 100 U/ml human IL-2 IS (Miltenyi Biotec), 1 ng/ml IL-15 (Miltenyi Biotec) ...
-
bioRxiv - Molecular Biology 2023Quote: ... 100 ng/ml IL-2 (#130-097-744, Miltenyi Biotec) for natural killer cells.
-
bioRxiv - Immunology 2024Quote: ... PepTivator® SARS-CoV-2 Prot_S-peptide pool (Miltenyi Biotec, consisting of 15-mer sequences with 11 amino acids overlap covering the complete protein coding sequence (aa 5–1273 ...
-
bioRxiv - Molecular Biology 2024Quote: ... 2% MACS NeuroBrew-21 (1x) (Miltenyi Biotec, 130-093-566), 0.5 mM L-Glutamine (Lonza ...
-
bioRxiv - Cell Biology 2021Quote: ... erythrocytes infected with NF54 wild type parasites (500µl packed red blood cells in culture, 6% parasitemia) were purified using magnetic activated cell sorting (QuadroMACS™, Miltenyi Biotec). Purified late stages were accompanied with 30 µl uninfected red blood cells and cultured for 4h to let the cells recover ...
-
bioRxiv - Cancer Biology 2022Quote: Fresh tumor tissue was minced to 2-4 mm sized pieces in a 6-cm dish and subsequently digested to single cell suspension using the Multi Tissue Human Tumor Dissociation Kit 1 (Miltenyi Biotec) and a gentleMACS OctoDissociator (Miltenyi Biotec ...
-
bioRxiv - Cancer Biology 2022Quote: ... and fresh tissue was minced to 2-4 mm sized pieces in a 6-cm dish and subsequently digested to single cell suspension using Multi Tissue Mouse Tumor Dissociation Kit 1 (Miltenyi Biotec) and a gentleMACS OctoDissociator (Miltenyi Biotec ...
-
bioRxiv - Immunology 2022Quote: ... supplemented with cytokines (IL-6 10ng/ml, IL-3 20ng/ml, M-CSF 50ng/ml and SCF 20ng/ml; all Miltenyi Biotec), with 2.5% Pen/Strep and 20μM L-Glutamine (Sigma-Aldrich) ...