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Citations for Miltenyi Biotec :
251 - 300 of 754 citations for 6 Chloro imidazo 1 2 a pyridine 2 carbaldehyde since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... were co-transferred with CD3+ cell-depleted autologous PBMCs (2 × 106) isolated using CD3 Microbeads and the LD Column (Miltenyi Biotec) into 5- to 7-week-old female mice ...
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bioRxiv - Microbiology 2021Quote: ... at a ratio of one bead per cell for 72 h and maintained in RPMI with 100 U/mL IL-2 (AIDS Reagents Program) and 100 ng/mL IL-7 (Miltenyi Biotec). Monocyte-derived-macrophages (MDMs ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated for 2 min at room temperature in the presence of red blood cell lysis buffer (Miltenyi Biotec S.I.) according to the manufacturer’s instructions ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... Lung cells were centrifuged and resuspended with fresh media with 20 μL/mL of PeptTivator® SARS-CoV-2 Prot_S (Miltenyi Biotec) and 5 μg/mL Brefeldin A (BioLegend) ...
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bioRxiv - Immunology 2020Quote: ... CD8+ T cells and CD4+ T cells were isolated from splenocyte suspensions using MACS MicroBeads (CD8a [Ly-2] and CD4 [L3T4] [Miltenyi Biotec]) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... CD8+ T cells and CD4+ T cells were isolated from splenocyte suspensions using MACS MicroBeads (CD8a [Ly-2] and CD4 [L3T4] [Miltenyi Biotec]) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... The organs were homogenized in 2 ml PBS using gentleMACS M tubes and gentleMACS Octo Dissociator (Miltenyi Biotec, Bergisch Gladbach, Germany). Afterward ...
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Single cell transcriptomics reveals the effect of PD-L1/TGF-β blockade on the tumor microenvironmentbioRxiv - Genomics 2020Quote: ... A red blood cell lysis step was performed by incubating cells for 2 minutes with 1x Red Blood Cell Lysis Solution (Miltenyi Biotec). Cells were washed again and then cell viability was measured using the Nexcelom Cellometer K2 Fluorescent Viability Cell Counter and the ViaStain AOPI Staining Solution (Nexcelom) ...
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bioRxiv - Microbiology 2020Quote: ... Each section was weighed and then homogenized in 2 ml PBS in gentleMACS M tubes and a gentleMACS Octo Dissociator (Miltenyi Biotec) using the RNA_1 setting ...
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bioRxiv - Immunology 2020Quote: ... PBMCs from 48 healthy individuals (samples taken before the SARS-CoV-2 outbreak) were enriched for CD19+ cells with CD19-microbeads (Miltenyi Biotec). For each individual ...
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bioRxiv - Immunology 2022Quote: B cells were purified from the spleens of mice (aged 2–7 months of age) through negative selection with anti-CD43 magnetic beads (Miltenyi Biotec) and subsequently cultured in RPMI 1640 media (Life Technologies ...
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bioRxiv - Immunology 2023Quote: ... T cells were stimulated by the addition of 100 IU/mL IL-2 (Proleukin) and anti-CD3/CD28 stimulation using loaded MACSiBead particles (Miltenyi Biotec) in a proportion of 1:1 of beads to cell ...
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bioRxiv - Bioengineering 2023Quote: ... and 2/3 for CD4+ subsets (both positive selection for CD4+CD25+cells and negative selection for CD4+CD25- cells Miltenyi Biotec). A small portion of each cell fraction was analyzed by flow cytometry to confirm enrichment of the desired cell subtype ...
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bioRxiv - Immunology 2023Quote: ... and incubated at 37°C for total of 30 minutes (15 minutes, 2 times) with rotated using MACSmix Tube Rotator (Miltenyi Biotec). Between and after the two incubations ...
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bioRxiv - Immunology 2023Quote: ... splenocytes were resuspended in CTL test media (Immunospot) and incubated at 37 °C for 2 hours with either no stimulation or S1 peptide simulation (Miltenyi Biotec) at a final concentration of 2 µg/mL before adding GolgiPlug to all samples for 4 additional hours ...
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bioRxiv - Bioengineering 2023Quote: Lung cells were centrifuged and resuspended with fresh complete RPMI media with 20 μL/mL of PeptTivator® SARS-CoV-2 Prot_S (Miltenyi Biotec) and 5 μg/mL Brefeldin A (Biolegend) ...
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bioRxiv - Synthetic Biology 2023Quote: ... supplemented with 10% Human AB serum (Gemini Bio Products) with the addition of 100 U/ml human IL-2 IS (Miltenyi Biotec), 1 ng/ml IL-15 (Miltenyi Biotec) ...
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bioRxiv - Immunology 2023Quote: ... Sarcoma tissue was collected on ice and immediately cut into 2 mm3 segments and dissociated to a single-cell suspension using the Human Tumor Dissociation Kit and gentleMACS dissociator (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... cells were stained with ITGA7-APC and ITGA5-PE (Supplementary Table 2) and sorted using a MACSQuant Tyto Cell Sorter (Miltenyi Biotec). Flow cytometry was performed regularly during SC proliferation using a MACSQuant10 Flow Analyzer (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... A total of 1.5 × 106 PBMCs were stimulated with 1 µg/mL of peptide pools for 6 h in TexMACS™ GMP Medium (Miltenyi Biotec), supplemented with 5% AB human serum (Sigma-Aldrich ...
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bioRxiv - Synthetic Biology 2020Quote: ... Dynabead-EV samples were re-suspended into 98 μl DPBS and 2 μl of one of the following antibodies: IgG1-PE (#130-113-200, Miltenyi Biotec, Germany), CD9-PE ...
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bioRxiv - Cancer Biology 2021Quote: Surgical specimens placed immediately in cold sterile DMEM after excision were cut into 2-4 mm pieces before undergoing enzymatic and mechanical dissociation using the gentleMACS™ Octo Dissociator multitissue kit and protocol (Miltenyi Biotec). Resuspended cells were then promptly plated into flasks ...
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bioRxiv - Bioengineering 2021Quote: ... Nalm6 tumors were extracted on day 4 post bacteria treatment (day 2 post T cell treatment) and lymphocytes were isolated from tumor tissue by mechanical homogenization using a gentleMACS™ Dissociator (Miltenyi Biotec) in complete hTcm ...
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bioRxiv - Microbiology 2022Quote: ... 1 ml of each sample was transferred to a 2 ml Eppendorf tube and the samples were assayed on a flow cytometer (MACSQuant® VYB, Miltenyi Biotec). Green fluorescent cells were detected using the blue laser (488 nm ...
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bioRxiv - Microbiology 2022Quote: ... and stimulated with either SARS-CoV-2 S-trimer (10-100 µg/mL) or SARS-CoV-2 PepTivator® Peptide S and NP protein Pools (10 µg/mL each, Miltenyi Biotec) for 72 h at 37°C ...
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bioRxiv - Developmental Biology 2022Quote: ... The remaining cells were pelleted and resuspended in 80 µl of MACS buffer (PBS pH 7.2, 0.5% BSA and 2 mM EDTA) and 20 µl anti-CD45 MicroBeads (Miltenyi Biotec #130-052-301) or 20 µl anti-F4/80 MicroBeads (Miltenyi Biotec #130-110-443 ...
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bioRxiv - Biochemistry 2021Quote: ... The cell pellet was resuspended in 80 μL of 2% BSA/PBS and incubated with 20 μL of anti-PE MicroBeads UltraPure (Miltenyi Biotec, USA.) for 15 min at 4°C ...
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bioRxiv - Immunology 2021Quote: ... 2 mM EDTA in PBS) before positive selection of CD14+ monocytes by incubation with magnetic CD14 microbeads (Miltenyi Biotec, 130-050-201) (15 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Tumors were cut in small pieces of approximately 2 mm3 then incubated with Human tumor dissociation kit (Miltenyi Biotec, #130-095-929) and placed at 37°C in agitation (500 rpm ...
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bioRxiv - Cell Biology 2023Quote: ... cut longitudinally into roughly 2-mm-long pieces and were isolated using the Liver Dissociation Kit (mouse) and GentleMACS Dissociator (Miltenyi Biotec, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... tissues were cleansed in ice-cold RPMI1640 and dissociated using the Multi-tissue dissociation kit 2 (Catalog No.130-110-203, Miltenyi Biotec, USA) following the manufacturer instructions ...
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bioRxiv - Cell Biology 2024Quote: ... + 0.5x GlutaMax (Thermo 35050061) + 1x NEAA (Thermo 11140050) + 1x N-2 (Thermo 17502048) + 1x MACS NeuroBrew-21 (Miltenyi Biotec 130-093-566). On D3 ...
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bioRxiv - Microbiology 2024Quote: ... 6 μl anti-DYKDDDDK microbeads (Miltenyi Biotec, 130-101-591) were added to 140 μl cell lysates and incubated for 30min on ice ...
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bioRxiv - Cell Biology 2022Quote: ... 2 mg of each cell lysate was incubated with 40 μL Anti-HA Microbeads (μMACS HA Isolation Kit, Miltenyi Biotec, 130-091-122) in a volume of 500 μL for 45 min at 4 °C with rotation ...
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bioRxiv - Developmental Biology 2020Quote: ... cell suspension with PBS supplemented with 0.5% BSA and 2 mM EDTA was applied to an MS column (Miltenyi Biotec, 130-042-201). After negative cells flow through the column ...
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bioRxiv - Neuroscience 2023Quote: ... this tissue was transitioned to a 2 ml Dounce homogenizer containing 1.5 ml of nuclei extraction buffer (130-128-024, Miltenyi Biotec, Bergisch Gladbach, Germany) along with 0.2 U/ml Ribolock RNase inhibitor ...
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bioRxiv - Immunology 2021Quote: ... splenocytes were isolated from C57BL/6 mice (male, 6-12 weeks old) and enriched using a CD4+ T Cell Isolation Kit (Miltenyi Biotec, No:130-104-453). Quality control was performed by flow cytometry (CD4+ [clone RM4-5 ...
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bioRxiv - Cancer Biology 2019Quote: ... tumor samples were cut into pieces of 2-4 mm and suspended using the tumor dissociation protocol with the GentleMacs® dissociator (Miltenyi Biotec GmbH, Germany). The cell suspension was applied to a cell strainer (70 μm ...
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bioRxiv - Immunology 2020Quote: ... The cell suspensions were incubated with running buffer (PBS supplemented with 0.5% FCS and 2 mM EDTA) and L3T4 microbeads (Miltenyi Biotec, Maquarie Park, NSW, Australia) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... samples were cut into approximately 2 – 4mm chunks with forceps and a scalpel and transferred to a gentleMACS C tube (Miltenyi Biotec, 130-093-237) containing 4.7 ml RPMI 1640 base media ...
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bioRxiv - Immunology 2022Quote: ... γδT cells were washed three times before co-culturing at a ratio of 1:5 with CD8+T cells isolated from tumor-bearing mice using CD8a (Ly-2) microbeads through magnetic activated cell sorting (MACS) (Miltenyi Biotec, Bergisch Gladbach, Germany). Tumor cell lysate (TCL ...
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bioRxiv - Plant Biology 2022Quote: ... Extracted protein samples were centrifuged at 20 000g for 2 min at 4°C and the supernatant was collected for immunoprecipitation using the µMACS GFP Isolation Kit (Miltenyi Biotec, MediRay, New Zealand). Total and immunoprecipitated proteins were resolved on a 4–12% SDS-PAGE gel ...
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bioRxiv - Developmental Biology 2023Quote: ... the dorsolateral neocortex was dissected and a single cell suspension was produced from 2-3 hemispheres per condition using the MACS Neural Tissue Dissociation kit containing papain (Miltenyi Biotec, 130-092-628). Cells were fixed in 1% formaldehyde for exactly 10 min on a rotating wheel (10 rpm ...
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bioRxiv - Cancer Biology 2023Quote: ... mixture at room temperature for 30 minutes and subsequently adding into 10 mL of RPMI+10%FBS and 2 uL of IL4 (Miltenyi Biotec 130-106-196). Cells were seeded at 0.5 × 106 per mL of CD40 stimulation medium at 37 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... Cerebral cortex was minced to small 2-3 mm pieces and dissociated using Neural Tissue Dissociation Kit P compounds (Miltenyi Biotec, 130-092-628) to obtain a cell suspension ...
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bioRxiv - Cancer Biology 2023Quote: Tumors from orthotopic PDAC mice were minced into 2-4 mm pieces and added with an enzyme mix from a tumor dissociation kit (Miltenyi Biotec, Bergisch Gladbach, Germany) in Roswell Park Memorial Institute medium 1640 (Cytiva ...
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bioRxiv - Immunology 2023Quote: ... the cells were washed with PBS and resuspended in 50 µl of 1X annexin binding buffer containing 2 µl of FITC-annexin V (Miltenyi Biotec, 130-092-052). Cells were then incubated at room temperature for 30 min in the dark ...
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bioRxiv - Cancer Biology 2024Quote: ... frozen single cell suspensions from MDOTS processing (see above) were thawed and CD8+ TILs were isolated using Cd8a (LY-2) Microbeads (Miltenyi Biotec, 130-117-044). T cells were cultured in RPMI (Corning ...
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bioRxiv - Immunology 2020Quote: ... Anti-IL-10 (REA842) and anti-IL-6 (REA1037, Miltenyi Biotec) were used for intracellular staining.
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... Cells were centrifuged and resuspended with fresh media with 40 μL/mL of PeptTivator® SARS-CoV-2 Prot_S (Miltenyi Biotec, Cat# 130-126-700), 1 μL/mL monensin (BioLegend) ...