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Citations for Miltenyi Biotec :
251 - 300 of 800 citations for 6β Fluoro 21 hydroxypregna 1 4 9 11 16 tetraene 3 20 dione 21 acetate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... cDCs were positively selected from PBMCs with CD141 (BDCA-3) MicroBead Kit (130-090-512, Miltenyi Biotec) by MACS technology (Figure Supplementary 6C) ...
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bioRxiv - Immunology 2024Quote: ... lung tissue from young (3-month-old) C57BL/6 mice was dissociated with the gentleMACS (Miltenyi Biotec) system and incubated at 37°C with Liberase™ for 1 hour ...
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bioRxiv - Immunology 2021Quote: ... and incubated for 45 mins at 4°C on a MACSmix Tube Rotator (Miltenyi Biotec). Anti-CD24 antibody-bound cells were then magnetically removed ...
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bioRxiv - Bioengineering 2021Quote: ... for 15 mins at 4°C and separated using a MACS Pro Separator (Miltenyi Biotec). Monocytes were cultured using Iscove’s Modified Dulbecco’s media supplemented with 10% human serum blood type AB ...
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bioRxiv - Immunology 2020Quote: ... plus 10ng/ml recombinant human IL-2 and IL-4 (Miltenyi Biotec, Auburn, CA, USA). Cells were maintained in a humidified incubator at 37°C with 5% CO2 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... falciparum culture (4% hematocrit, 5-10% parasitemia) was passed through an LS column (Miltenyi Biotec; previously rinsed with 2 mL culture medium ...
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bioRxiv - Bioengineering 2023Quote: ... Tumor digestion protocol #4 was performed by using the gentleMACS Octo tissue dissociator (Miltenyi Biotec) with three 10 min rounds of processing over 40 min ...
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bioRxiv - Cancer Biology 2023Quote: ... The cell pellets were resuspended with permeabilization buffer and incubated with 20 μl FcR blocking reagent (Miltenyi Biotec) for 10 min at 4°C in the dark ...
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bioRxiv - Immunology 2021Quote: ... Th1 cultures were activated with CD2/3/28 activation beads (Human T cell Activation/Expansion kit, Miltenyi Biotec) at a 2:1 ratio of cells to beads with anti-IL-4 (2μg/mL) ...
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bioRxiv - Immunology 2023Quote: ... and subsequently expanded using a rapid expansion protocol based on CD3-TCR-activation via OKT-3 (Miltenyi Biotec) and irradiated donor-mismatched PBMCs (all CAR-T cells ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were stained for 15min at 4°C and then transferred to LS columns (Miltenyi Biotec). Subsequently ...
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Single cell transcriptomics reveals the effect of PD-L1/TGF-β blockade on the tumor microenvironmentbioRxiv - Genomics 2020Quote: ... and the tissue was stored overnight at 4°C in MACS Tissue Storage Solution (Miltenyi Biotec). Tumor tissue was minced and dissociated using the mouse Tumor Dissociation Kit (Miltenyi Biotec ...
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bioRxiv - Systems Biology 2023Quote: ... incubated for 15 minutes at 4°C and transfered in conditioned Whole Blood Columns (Miltenyi Biotec) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... IL-4 (500 U/ml) and GM-CSF (800 U/ml, #130-093-868, Miltenyi Biotec) in a humidified ...
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bioRxiv - Immunology 2024Quote: ... supplemented with 1000 IU/ml GM-CSF and 400 IU/ml IL-4 (both Miltenyi Biotec) for 5 days ...
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bioRxiv - Cell Biology 2024Quote: ... Intracellular markers (OCT3/4, NANOG) were stained by fixation/permeabilization solution (Miltenyi Biotec, 130-093-142) (30 min ...
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bioRxiv - Neuroscience 2021Quote: ... Nuclei pellets were washed tree times with one filtration step by MACS pre-separation filter 20 μm (Miltenyi Biotec). Nuclei pellets were resuspended in 100 μL of staining buffer (DPBS BSA 2% ...
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bioRxiv - Genomics 2019Quote: ... and Anti-Biotin MicroBeads UltraPure were added at a 20% concentration by volume (Cat.# 130-105-637, Miltenyi Biotec) and incubated at room temperature for 30 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... the cell pellet re-suspended in 20 μl of anti-PE magnetic-activated cell sorting (MACS) microbeads (Miltenyi Biotec) with 80 μL separation buffer and incubated in the dark at 4°C for 30 min ...
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bioRxiv - Neuroscience 2020Quote: ... single cell iPSCs were seeded at 3×105 cells/cm2 in StemMAC iPS-Brew (Miltenyi Biotec, Bergisch Gladbach, DE) in presence of 10 µM Rock-Inhibitor Y-27632 (Cell Guidance Systems ...
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bioRxiv - Immunology 2023Quote: ... Human peripheral blood mononuclear cells purchased from STEMCELL (70025) was stimulated by OKT-3 (Miltenyi Biotec, 130-093-387) and CD28 (BD Pharmingen ...
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bioRxiv - Immunology 2020Quote: Bone marrow cells were cultured (0.5×106 cells per mL) in the presence of 20 ng/mL GM-CSF (Miltenyi Biotec) in complete RPMI medium ...
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bioRxiv - Cancer Biology 2020Quote: ... Thawed samples were counted and a maximum of 107 cells resuspended in 100µl PBS and incubated on ice for 20 min with 1µl Viobility-405/520 amine reactive viability dye (Miltenyi Biotec Norden AB ...
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The antibiotic bedaquiline activates host macrophage innate immune resistance to bacterial infectionbioRxiv - Immunology 2019Quote: ... and were allowed to differentiate into Mφs in the presence of granulocyte macrophage colony-stimulating factor (GM-CSF, 20 ng/mL; Miltenyi Biotec) over a 6-day period ...
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bioRxiv - Developmental Biology 2023Quote: ... The cells were resuspended in 180 μl artificial seawater containing 0.05% BSA and we added 20 μl of CD4 antibody (Miltenyi Biotec) conjugated with magnetic beads ...
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bioRxiv - Neuroscience 2023Quote: ... and filtered resuspended nuclei with 20 um strainer (pre-separation filter; catalog # 130-101-812; Miltenyi Biotec; Bergisch Gladbach, Germany). We fluorescently labeled nuclei with propidium iodide and then quantified them with a DeNovix CellDrop automated cell counter ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were cultured in clusters and passaged every 4-5 days using StemMACS Dissociation reagent (Miltenyi Biotec). All iPS lines were genotyped using an RFLP at the relevant important loci and the sequence was confirmed by Sanger sequencing ...
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bioRxiv - Immunology 2021Quote: ... cut into 4 pieces and enzymatically digested by using the whole skin dissociation kit from Miltenyi biotec without enzyme P for 5 hours at 37°C under agitation (500 rpm) ...
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bioRxiv - Immunology 2021Quote: ... Fc receptors were blocked for 10 minutes at 4°C with FcR blocking reagent (MACS Miltenyi Biotec). Cells harvested during the conversion period from CD34+ hematopoietic precursors to CD14+ monocytes (Days 12-21 of the differentiation protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were stained for 15min at 4°C and then transferred to an LS column (Miltenyi Biotec). Human cells were then cytospinned with Shandon cytofunnels (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed with HBSS and spun at 300 g for 5min at 4 °C (Miltenyi Biotec). The pellet was resuspended and incubated with 80 µL of MACS buffer and 20 µL of anti-IgM microbeads (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were incubated at 4 with magnetic microbeads conjugated to monoclonal anti-human CD14 Abs (Miltenyi Biotec) and isolated by positive magnetic selection according to manufacturer’s instructions (Miltenyi Biotec) ...
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bioRxiv - Developmental Biology 2022Quote: Soft palatal tissue of E14.5 C57BL/6J mouse embryos was dissected and digested using Multi Tissue Dissociation Kit 3 (130-110-204, Miltenyi Biotec) at 37°C with shaking at 600 rpm for 15 minutes in a Thermomixer (2231000269 ...
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bioRxiv - Developmental Biology 2022Quote: Soft palatal tissue of E14.5 C57BL/6J mouse embryos was dissected and digested using Multi Tissue Dissociation Kit 3 (130-110-204, Miltenyi Biotec) at 37°C with shaking at 600 rpm for 15 minutes in a Thermomixer (2231000269 ...
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bioRxiv - Immunology 2022Quote: ... (3) Non-SARS-CoV-2 antigens: Peptide pools of the pp65 protein of human cytomegalovirus (CMV) (Miltenyi Biotec, Gladbach, GER), or HIV-1 gag peptide pools (NIH AIDS Reagent Repository ...
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bioRxiv - Neuroscience 2023Quote: ... frozen hippocampus of 3 mice in each sample group were dissociated using the Adult Brain Dissociation kit (Miltenyi Biotec, Germany), and after dead cell removal ...
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bioRxiv - Cancer Biology 2023Quote: ... and 3) removal of debris and dead cells using the Dead Cell Removal Kit (Miltenyi Biotec, Cat: 130-090-101). The brain of an age-matched uninoculated mouse was harvested and processed in parallel to serve as the negative control for downstream FACS sorting ...
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bioRxiv - Cancer Biology 2024Quote: ... tumors were harvested from mice on Day 3 and separately dissociated on a GentleMacs Octo Dissociator with Heaters (Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Bioengineering 2024Quote: ... Thermo) were washed 3 times with PBS followed by incubation with biotin anti-mouse CD63 antibody (Clone REA563; Miltenyi Biotec) or biotin anti-mouse fibronectin (FN ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were washed in 1%FBS in Dulbecco’s PBS 3 times before straining through 75 µm and 30 µm strainers (Greiner Bio-One and Miltenyi Biotec) in Dulbecco’s PBS ...
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bioRxiv - Cell Biology 2020Quote: ... and stimulated for 18 h with IL-2 (20 ng/ml; National Cancer Institute Preclinical Repository) and/or IL-12 (10 ng/ml; Miltenyi Biotec) cytokines ...
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bioRxiv - Bioengineering 2021Quote: ... we suspended pelleted PBMCs in the sorting buffer (80 μL for 107 cells) with CD3 magnetic microbeads (20 μL for 107 cells) from Miltenyi Biotec for 15 min at 4 °C according to the capacity of the microbeads ...
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bioRxiv - Physiology 2021Quote: ... The pellets were resuspended in 100 μl beads buffer consisting of 80 μl sorting buffer with 20 μl secondary Anti-rabbit IgG MicroBeads (Miltenyi Biotec) and incubated for 15 min in at 4 °C sorting buffer (900 μl) ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... Lung cells were centrifuged and resuspended with fresh media with 20 μL/mL of PeptTivator® SARS-CoV-2 Prot_S (Miltenyi Biotec) and 5 μg/mL Brefeldin A (BioLegend) ...
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bioRxiv - Microbiology 2022Quote: ... Stained and MACS bead-bound bacteria unbound to magnet plate were collected (20∼40 μl) and passed through MACS molecular columns (Miltenyi Biotec) (one sample/column ...
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bioRxiv - Neuroscience 2021Quote: ... Nuclei pellets were washed tree times in staining buffer with one filtration step by MACS pre-separation filter 20 μm (Miltenyi Biotec) to a final resuspension in 300 μl of staining buffer for Malassez cell counting with Trypan blue counterstaining (Trypan Blue solution ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were successively filtered through nylon filters (100 µm and 20 µm) followed by a percoll gradient and MACS sorting with CD45 Microbeads (Miltenyi Biotec). Cells were stained with APC anti-human EpCAM antibody (Biolegend ...
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bioRxiv - Microbiology 2023Quote: ... 0.01% BSA and 40 U/ml RNase inhibitor) and filter through 20 µm pre-separation filters (Miltenyi Biotec, 130-041-407) and were centrifuged with a swing bucket at 500xg ...
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bioRxiv - Neuroscience 2023Quote: ... Nuclei pellets were washed three times in staining buffer with one filtration step by MACS pre-separation filter 20 μm (Miltenyi Biotec) to a final resuspension in 300 μL of staining buffer for Malassez cell counting with Trypan blue counterstaining (Trypan Blue solution ...
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bioRxiv - Bioengineering 2023Quote: Lung cells were centrifuged and resuspended with fresh complete RPMI media with 20 μL/mL of PeptTivator® SARS-CoV-2 Prot_S (Miltenyi Biotec) and 5 μg/mL Brefeldin A (Biolegend) ...