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Citations for Miltenyi Biotec :
251 - 280 of 280 citations for 5 Pyrimidinecarbonitrile 4 ethoxy 6 trifluoromethyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... tumor samples were cut into pieces of 2-4 mm and suspended using the tumor dissociation protocol with the GentleMacs® dissociator (Miltenyi Biotec GmbH, Germany). The cell suspension was applied to a cell strainer (70 μm ...
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bioRxiv - Microbiology 2020Quote: ... to analyze pDCs for 20 min at 4°C to analyze B cells or with two antibodies directed against CD303(BDCA2)-APC (Miltenyi Biotec, #130-097-931) and CD304(BDCA4)-PE (Miltenyi Biotec # 130-112-045) ...
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bioRxiv - Developmental Biology 2022Quote: ... The cells are centrifugated at 600 g for 7 min at 4 °C and resuspended with 180 μl running buffer (Miltenyi Biotec, 130-091-221) for MACS selection.
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bioRxiv - Immunology 2019Quote: ... centrifuged at 500g for 10 min at 4 °C and resuspended in 0XTD supplemented with 100 ng/ml each of Stem Cell Factor (SCF, Miltenyi Biotec, 130-096-695), Flt3 Ligand (Flt3L ...
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bioRxiv - Plant Biology 2022Quote: ... Extracted protein samples were centrifuged at 20 000g for 2 min at 4°C and the supernatant was collected for immunoprecipitation using the µMACS GFP Isolation Kit (Miltenyi Biotec, MediRay, New Zealand). Total and immunoprecipitated proteins were resolved on a 4–12% SDS-PAGE gel ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.4 x 106 isolated T cells from the same donor and 0.4 x 106 T cell activation beads (Miltenyi Biotec, anti-CD2/3/28) were added ...
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bioRxiv - Cancer Biology 2023Quote: ... For CD8+ T cells magnetic isolation we incubated the cell suspension 15 min incubation at 4°C with CD8 microbeads (Miltenyi Biotec, #130-045-201), then cells were passed through LS magnetic columns (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2023Quote: ... 2-4 mm pieces of tumors from huPDAC mice were treated with an enzyme mix from a tumor dissociation kit (Miltenyi Biotec, Bergisch Gladbach, Germany). The tissues were dissociated using a gentle MACS Dissociator (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2023Quote: Tumors from orthotopic PDAC mice were minced into 2-4 mm pieces and added with an enzyme mix from a tumor dissociation kit (Miltenyi Biotec, Bergisch Gladbach, Germany) in Roswell Park Memorial Institute medium 1640 (Cytiva ...
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bioRxiv - Cancer Biology 2020Quote: ... and subsequently stained at 4°C with antibodies against following human antigens: CD3 (REA613, APC-Vio770, Miltenyi Biotec, Cat no.130-113-698, 1:50), CD4 (A161A1 ...
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bioRxiv - Immunology 2022Quote: ... Cells were labeled for at least 20 minutes at 4 ° C and CD14+ cells were isolated by magnetic column (Miltenyi Biotec, catalog# 130-042-201).
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bioRxiv - Immunology 2024Quote: ... single cell suspensions of mouse lungs were generated by putting lungs in gentle MACS™ C tubes and mechanically homogenizing in 5 ml PBS using a gentle MACS™ Octo Dissociator (Miltenyi Biotec) in the absence of enzymes ...
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bioRxiv - Developmental Biology 2024Quote: ... The cells were pelleted and resuspended in FACS buffer for immunomagnetic cell separation with EpCAM-conjugated beads (Miltenyi Biotec, Catalog No. 130-105-958, 1/4). After 20 min incubation at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Cells were centrifuged at 400 g for 5 min and incubated with 1:25 diluted mouse FcR blocking reagent (Miltenyi Biotec, 130-092-575) in FACS Buffer (PBS ...
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bioRxiv - Immunology 2021Quote: CD4+ T cells were isolated from at least 5×106 PBMCs on the day of thaw by negative selection using the CD4+ T cell isolation kit II (Miltenyi Biotec, Bergisch Gladbach, Germany) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: Oligodendrocyte precursor cells (OPCs) were isolated from 5–7-day old YACQ84 transgenic mice using magnetic cell sorting (MACS, Miltenyi Biotec, Bergisch Gladbach, Germany). Whole brains were dissected and homogenized using the Neural Tissue Dissociation Kit P (Miltenyi ...
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bioRxiv - Neuroscience 2021Quote: ... a 5 µl volume of phosphate-buffered PBS (PBS) containing 10 μg/kg/injection of recombinant mouse IL-1β (Miltenyi Biotec, Bergisch Gladbach, Germany) or of PBS alone (control ...
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bioRxiv - Genetics 2022Quote: ... in a cell culture incubator (37 °C; 5% CO2) followed by dissociation in gentleMACS C Tubes (Miltenyi Biotec Inc., cat. no. # 130-093-237) in a total of 8 ml DMEM media with 10% FBS using gentleMACS Dissociator Program A twice and seeded onto 15 cm tissue culture dishes and grown in DMEM with 10% FBS at 37 °C with 5% CO2.
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bioRxiv - Developmental Biology 2019Quote: ... NeutrAvidin-functionalized devices were incubated with either (1) 2 μg/ml biotinylated mouse anti-human anti-SSEA-5 antibody (Miltenyi Biotec, Bergisch Gladbach, Germany) (2 ...
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bioRxiv - Cell Biology 2023Quote: ... The tissue pieces were transferred to a 5 ml conical tube containing Mix 1 from the Neural Tissue Dissociation Kit (Miltenyi Biotec, 130-092-628) and incubated on a shaker for 15 minutes at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... 10-20 cortices of PN day 2-4 pups were dissociated using the MACS Neural Tissue Dissociation Kit-T (Miltenyi Biotec, Cat# 130-093-231, Auburn, CA) at 37°C (5% CO2 ...
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bioRxiv - Immunology 2020Quote: ... Nylon wool-enriched splenic T cells from naive or immunized mice were incubated sequentially for 10 min at 4°C with FITC-conjugated anti-mouse γδ TCR or αβ TCR Abs and 15 min at 4°C with anti-FITC Microbeads (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany), then the cells were separated into bound and non-bound fractions on an autoMACS™ separator column (Miltenyi Biotec GmbH) ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was coupled with Anti-tomm22 micro-Beats (30min at 4° C) to remove free mitochondria through Magnetic-assisted cell sorting (MACS) (Miltenyi Biotec, 130-096-946, 130-042-401). The mitochondria-depleted flow-through was centrifuged at 13,000 × g for 10 min at 4° C to pellet synaptosomes (100ul of PBS resuspension ...
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bioRxiv - Cell Biology 2020Quote: ... LT-HSCs were enriched by positive selection for c-Kit expression using anti-CD117 immunomagnetic beads (130-091-224, Miltenyi Biotec Inc. 1:5 dilution). Second ...
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bioRxiv - Immunology 2020Quote: ... BM cells were incubated on ice for 20 minutes with c-Kit microbeads (Miltenyi Biotec, 130-091-224; 5 μl/100 μl SM per mouse), washed with SM and enriched on an AutoMACS Pro magnetic cell separator (Miltenyi Biotec) ...
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bioRxiv - Developmental Biology 2022Quote: ... and cells were resuspended in 5 mL MACS Buffer (autoMACS Rinsing Solution [Miltenyi Biotec, 130-091-222] with MACS BSA Stock Solution [Miltenyi Biotec, 130-091-376]) and passed through a 40 μm filter (Greiner Bio-One ...
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bioRxiv - Immunology 2023Quote: ... Each lung was then fragmented using surgical scissors and transferred to 5 mL of dissociation media (1 lung per dissociation reaction) in gentleMACS™ C Tubes (Miltenyi Biotec, 130-093-237). Dissociation media contained 0.5 U/mL dispase (Stemcell Technologies ...
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bioRxiv - Pathology 2022Quote: ... and cells were stained for 30 min at 4°C with the following anti-human antibodies: PerCP-Vio700-conjugated REAfinity recombinant anti-CD31 (Miltenyi Biotec, clone REA730, #130-110-673, 1/20), BV421-conjugated anti-Tissue Factor/CD142 (BD Biosciences ...
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bioRxiv - Microbiology 2020Quote: ... antibody for 45 min at 4°C followed by the incubation with the secondary antibody anti-IgG1-PE (REA1017, Miltenyi Biotec, 1 μL/105 cells in 100 μL) for 30 min at 4°C ...
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bioRxiv - Neuroscience 2020Quote: Isolated mononuclear cells from five brains were then further purified by CD11b positive selection using MACS columns (N = 5 mice, >90% enrichment of CD11b+ microglia [25]) (Miltenyi Biotec, Cat. No. 130–093-636) to selectively enrich microglia and brain mononuclear phagocytes (Figure 1B) ...