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Citations for Miltenyi Biotec :
1 - 50 of 1104 citations for 3 4 Hydroxy 5 isopropyl 6 oxo 1 6 dihydro pyrimidin 2 ylsulfanyl propionic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... and interleukin 6 (IL-6) (Miltenyi Biotec), as previously reported 35,36 ...
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bioRxiv - Immunology 2020Quote: ... sequences [SLA (p1-p53)] or 0.6 nmol/mL PepTivatorR Candida albicans MP65 (peptides pools of 15 amino acids length with 11 amino acid overlap, Miltenyi Biotec) in 5% human serum RPMI medium in the presence of 1µg/ml anti-CD40 (HB14 ...
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bioRxiv - Biochemistry 2021Quote: ... supplemented with growth factors (Miltenyi Biotec, IL-3, IL-6 and G-CSF (10 ng/mL), h-SCF and FLt3-L (50 ng/mL) ...
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bioRxiv - Systems Biology 2021Quote: ... supplemented with growth factors (Miltenyi Biotec, IL-3, IL-6 and G-CSF (10 ng/mL), h-SCF and FLt3-L (50 ng/mL) ...
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bioRxiv - Systems Biology 2023Quote: ... supplemented with growth factors (Miltenyi Biotec, IL-3, IL-6 and G-CSF (10 ng/mL), h-SCF and FLt3-L (50 ng/mL) ...
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bioRxiv - Cancer Biology 2023Quote: ... recombinant human IL-6 (Miltenyi Biotec), recombinant human TPO (PeproTech) ...
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bioRxiv - Immunology 2023Quote: ... IL-6 (30ng/mL; Miltenyi Biotec), anti-IFN-γ (5µg/mL ...
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bioRxiv - Neuroscience 2023Quote: ... 2) 4.17 mM GadoSpin-P (n=6; MW=200 kDa; Miltenyi Biotec, Bergisch Gladbach, Germany); and 3 ...
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bioRxiv - Immunology 2024Quote: ... lung tissue from young (3-month-old) C57BL/6 mice was dissociated with the gentleMACS (Miltenyi Biotec) system and incubated at 37°C with Liberase™ for 1 hour ...
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bioRxiv - Immunology 2021Quote: ... A total of 1.5 × 106 PBMCs were stimulated with 1 µg/mL of peptide pools for 6 h in TexMACS™ GMP Medium (Miltenyi Biotec), supplemented with 5% AB human serum (Sigma-Aldrich ...
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bioRxiv - Immunology 2020Quote: ... IL-6 mAbs from Miltenyi Biotec (Paris, France), and TNFa mAb from BD Biosciences.
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bioRxiv - Immunology 2022Quote: ... supplemented with cytokines (IL-6 10ng/ml, IL-3 20ng/ml, M-CSF 50ng/ml and SCF 20ng/ml; all Miltenyi Biotec), with 2.5% Pen/Strep and 20μM L-Glutamine (Sigma-Aldrich) ...
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bioRxiv - Immunology 2020Quote: ... DC differentiation was carried out over 6 days with 250 U/ml IL-4 and 50ng/ml GM-CSF (Miltenyi Biotec).
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bioRxiv - Immunology 2023Quote: ... Cells from BRAFi (n = 5) or BRAFi/C-Me (n = 6) tumors were pooled and CD45+ cells were isolated using CD45 MicroBeads (mouse, Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... splenocytes were isolated from C57BL/6 mice (male, 6-12 weeks old) and enriched using a CD4+ T Cell Isolation Kit (Miltenyi Biotec, No:130-104-453). Quality control was performed by flow cytometry (CD4+ [clone RM4-5 ...
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bioRxiv - Immunology 2020Quote: ... Anti-IL-10 (REA842) and anti-IL-6 (REA1037, Miltenyi Biotec) were used for intracellular staining.
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bioRxiv - Immunology 2020Quote: ... Cells were stimulated by the addition of individual virus-specific peptide pools (1 µg/ml) for 6 h in the presence of a blocking CD40 antibody (1 µg/ml; Miltenyi Biotec). For subsequent MACS-based enrichment of CD154+ ...
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bioRxiv - Cancer Biology 2020Quote: Dorsal skin was removed from euthanized 4-week-old C57BL/6 mice and dissociated with the Epidermis Dissociation Kit (Miltenyi Biotec, Cat. 130-095-928) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: Fresh tumor tissue was minced to 2-4 mm sized pieces in a 6-cm dish and subsequently digested to single cell suspension using the Multi Tissue Human Tumor Dissociation Kit 1 (Miltenyi Biotec) and a gentleMACS OctoDissociator (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2022Quote: ... and fresh tissue was minced to 2-4 mm sized pieces in a 6-cm dish and subsequently digested to single cell suspension using Multi Tissue Mouse Tumor Dissociation Kit 1 (Miltenyi Biotec) and a gentleMACS OctoDissociator (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2022Quote: ... On day five ECs were dissociated with StemPro Accutase (diluted 1:6 in D-PBS) and separated via Magnetic-activated cell sorting (MACS, Miltenyi Biotec) using CD34 MicroBeads ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were resuspended in 1 ml 10% FBS in PBS and stained with 6 μg anti-mouse Ter119-PE antibody (Miltenyi Biotec) per 108 cells for 20 minutes at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were passaged (1:12) every 3-4 days using StemMACS Passaging Solution XF (Miltenyi Biotec) and Y-27632 dihydrochloride (Santa Cruz ...
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bioRxiv - Immunology 2020Quote: ... non-pathogenic Th17: 25ng/ml rmIL-6 and 2ng/ml of rhTGFb1 (Miltenyi Biotec); iTreg ...
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bioRxiv - Immunology 2023Quote: ... naïve T cells were cultured in the presence of IL-6 (30ng/mL; Miltenyi Biotec), soluble anti-CD28 (1µg/mL ...
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bioRxiv - Genomics 2024Quote: ... The EGFR-Hofbauer/fibroblast fraction was washed and incubated for 10 minutes at 4 °C in the dark with 1:11 REAfinity™ phycoerythrin (PE)-conjugated anti-CD10 antibody (anti-CD10-PE, Miltenyi Biotec, #130-114-5-2, lot #5190109168). The Hofbauer/fibroblast fraction was then washed and incubated for 15 minutes at 4 °C in the dark with 1:5 anti-phycoerythrin MACS® MicroBeads (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... and PE-conjugated anti-IL-4 (clone 7A3-3, Miltenyi Biotec) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2022Quote: PBMCs isolated from healthy donors (n=6) were used for CD4+T cell isolation kit (Miltenyi Biotec). CD4+T cells were treated with recombinant IL-2 ...
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bioRxiv - Immunology 2022Quote: CD8+ T cells were positively sorted with beads from the spleens of C57BL/6 mice (Miltenyi Biotec). The T cells were labelled with CFSE (Invitrogen Molecular Probes) ...
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bioRxiv - Immunology 2024Quote: ... DCs were purified (>90%) from the spleens of C57BL/6 mice using MACS CD11c microbeads (Miltenyi Biotec). Freshly isolated CD8+ T cells were then activated either with anti-CD3 and anti-CD28 antibody-coated microbeads at a 1:1 bead:cell ratio (Miltenyi Biotec ...
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bioRxiv - Microbiology 2022Quote: ... mature parasite cultures (6-8% parasitemia) were magnetically separated using magnetic columns (MACS LD columns, Miltenyi Biotec) and diluted to 1% parasitemia containing 0.5 mL fresh erythrocytes88 ...
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bioRxiv - Neuroscience 2021Quote: ... 4 °C and stained with the APC-labelled ACSA-2 antibody (1:10, Miltenyi Biotec, 130-102-315) in 0.5 % BSA in PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... and 5 ng/ml human FGF-2 (Miltenyi Biotec). Then ...
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bioRxiv - Cell Biology 2022Quote: ... JAM-1 and CD45 (Fig. 3, Supplementary Table 4) for 15 min prior to analysis on a MACSQuant10 Flow Analyzer (Miltenyi Biotec). To determine proportions of SCs and FAPs (Fig ...
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bioRxiv - Genomics 2020Quote: ... 1:100 B27 supplement without retinoic acid (Miltenyi Biotec) supplemented with 10 µM SB-431542 ...
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bioRxiv - Immunology 2022Quote: ... CD11c+ cells were sorted from pooled spleens of WT C57BL/6 mice by using mouse CD11c MicroBeads (Miltenyi Biotec). Then ...
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bioRxiv - Immunology 2020Quote: Neutrophils from bone marrow were purified from C57Bl/6 mice by negative selection using neutrophil isolation kit (Miltenyi Biotec). Purified neutrophils were cultured at 1.0-5.0 x 107 total cells and were infected or not at different ratios with RFP LmRyn metacyclic promastigotes ...
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bioRxiv - Immunology 2020Quote: ... cells were isolated from WT C57BL/6 spleens using a mouse naïve CD4+ T cell isolation kit (Miltenyi Biotec) and cultured as described previously (Shakya et al. ...
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bioRxiv - Cell Biology 2020Quote: ... Naïve CD4+ T cells were isolated from the lymph nodes and spleen of C57BL/6 mice by MidiMACS (Miltenyi Biotec). Cells were activated by plate-bound anti-CD3 (5 μg mL−1 ...
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bioRxiv - Cancer Biology 2022Quote: Primary NK cells were isolated and enriched the day of the experiment from the spleens of 8-12 week old female C57BL/6 mice using the NK Cell Isolation Kit II according to manufacturer’s instructions (Miltenyi Biotec). 50,000 NK cells were then seeded in the top chamber of a transwell insert (Corning ...
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bioRxiv - Immunology 2023Quote: Naïve CD4+ T cells were purified from spleen and lymph nodes of 6-15 week old 2D2 mice or OT-II mice using the naïve CD4+ T cell isolation Kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... pan splenic T cells were isolated from crushed spleens of naïve C57BL/6 mouse using the Mouse Pan T Cell Isolation Kit (Miltenyi Biotec). In the subsequent co-culture incubation ...
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bioRxiv - Immunology 2022Quote: ... cells were prepared from CD45.1 Thy1.2 C57Bl/6 mice by first crushing bones followed by T cell depletion using CD4 and CD8 MicroBeads (Miltenyi Biotec). CD45.2 Thy1.2 FoxP3/GFP+ CD4+ Treg were FACS sorted from CD8/CD19-depleted single cell suspensions from spleens and lymph nodes from CD45.2 Thy1.2 FoxP3GFP+ C57Bl/6 using a BD FACS Aria II ...
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bioRxiv - Immunology 2022Quote: Splenic iNKT cells were isolated from C57BL/6 mice sorting CD45+CD3+CD1d:PBS57Tet+ cells upon enrichment through B cells exclusion (Mouse CD19 microbeads, Miltenyi Biotec). Bone marrow derived dendritic cells (BMDCs ...
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bioRxiv - Physiology 2024Quote: ... Renal primary cells were obtained from 6 C57/Bl6 mice after mechanical dissociation performed using the gentleMACS dissociator (Miltenyi Biotec). About 80,000 cells were therefore seeded just after isolation into XF96 cell culture microplates (Agilent ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 and 4 cytokines were added as followed: 20 ng/ml IL-4 (#130-093-920, Miltenyi Biotec) and 100 ng/ml GM-CSF (#130-093-864 ...
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bioRxiv - Immunology 2021Quote: ... T cells were activated with T cell activation/expansion CD2/3/28 beads at a ratio of 1:2 bead to cells (Miltenyi Biotec). Cultures were maintained in HPLM media with 5% dialyzed FBS (Sigma Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... 1/3 for CD8+ selection (Miltenyi Biotec) and 2/3 for CD4+ subsets (both positive selection for CD4+CD25+cells and negative selection for CD4+CD25- cells Miltenyi Biotec) ...
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bioRxiv - Immunology 2021Quote: ... Cells were cultured for 18 h at 5×105 cells per well with 2 μg/ml of a peptide pool consisting mainly of 15-mers (overlapping by 11 amino acids) covering the immunodominant sequence domains of the SARS-CoV-2 Spike protein (PepTivator® SARS-CoV-2 Prot_S; Miltenyi Biotec, Germany). The number of spots was determined using an automatic ELISPOT reader and image analysis software (CTL-ImmunoSpot® S6 Micro Analyzer ...
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bioRxiv - Cell Biology 2021Quote: ... erythrocytes infected with NF54 wild type parasites (500µl packed red blood cells in culture, 6% parasitemia) were purified using magnetic activated cell sorting (QuadroMACS™, Miltenyi Biotec). Purified late stages were accompanied with 30 µl uninfected red blood cells and cultured for 4h to let the cells recover ...