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Citations for Miltenyi Biotec :
401 - 450 of 467 citations for tert Butyl 10 oxo 4 9 diazaspiro 4.5 decane 4 carboxylate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... each blood sample was diluted in a 1 to 1 ratio with the HBSS buffer solution and incubated with 10% EpCAM MicroBeads (Miltenyi Biotec) with shaking at room temperature for an hour ...
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bioRxiv - Immunology 2021Quote: ... T cell activation by peptide was assessed according to the expression of eGFP regulated by the NFAT promoter and analysed on a MACSQuant Analyzer 10 (Miltenyi Biotec). The acquired data was processed by FlowJo (version 10.6.2 ...
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bioRxiv - Synthetic Biology 2021Quote: ... All sample volumes were adjusted to 100 µl with 1x PBS + 10 µM ZnCl2 and measured via flow cytometry (MACSquant VYB, Miltenyi Biotec). The flow cytometer was calibrated using MacsQuant calibration beads (Miltenyi Biotec ...
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bioRxiv - Microbiology 2021Quote: ... were obtained by 7 days stimulation with 20 ng ml−1 macrophage colony-stimulating factor (M-CSF) and 10 ng ml−1 granulocyte-macrophage colony-stimulating factor (GM-CSF) (Miltenyi Biotec).
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bioRxiv - Microbiology 2022Quote: ... BSA in PBS + 0.2 mM EDTA (FACS buffer) and subsequently resuspended in 100 μl FACS buffer containing 10 % (v/v) FcR blocking reagent (Miltenyi Biotec) and 1 % (v/v ...
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bioRxiv - Immunology 2020Quote: ... the corresponding mAbs were added for 30 minute and cells were washed with BD Perm/Wash Buffer 1X before acquisition in the MACSQuant Analyzer 10 flow cytometer (Miltenyi Biotec). The percentage of positive cells for CD107a ...
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bioRxiv - Immunology 2020Quote: ... Sorted cell subsets were co-cultured at a ratio 1:1 in 200µL RPMI/10% FBS and stimulated with CytoStim™ (Miltenyi Biotec). After 7 days’ culture ...
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bioRxiv - Neuroscience 2020Quote: ... The cell pellet was washed with DMEM/10% FBS followed by CD11b positive selection (Miltenyi Biotec, Cat. No. 130–093-636) using mini-MACS (Miltenyi Biotec ...
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bioRxiv - Genomics 2020Quote: ... The cell suspension is pipetted through Falcon tubes with cell-strainer caps and then analyzed by MACSQuant Analyzer 10 Flow Cytometer (Miltenyi Biotec) for 10,000 events.
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bioRxiv - Immunology 2020Quote: ... anti-PDL1-PE/Cy7 and anti-FasL-APC to analyze surface markers on B cells involved in T cell suppression and with CD4-APC-Vio770 to investigate T cell proliferation by flow cytometry (MACSQuant Analyzer 10, Miltenyi Biotec).
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bioRxiv - Immunology 2021Quote: Splenic B cells were isolated from 10 to 12 weeks old MRL/Lpr mice using B Cell Isolation Kit (Miltenyi Biotec). Isolated cells were washed three times with PBS ...
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bioRxiv - Immunology 2021Quote: ... Cells derived from low-density fraction were stained using FITC-labeled anti-EpCAM mAb (dilution: 1/10, HEA-125, Miltenyi Biotec), PE-labeled anti-CD45 mAb (dilution ...
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bioRxiv - Immunology 2022Quote: ... in 96- well U-bottom plates 24 hours prior to co-culture with the indicated combinations of growth/differentiation factors (10 ng/ml each; all from Miltenyi Biotec). UCB or BM CD34+ HSPC subsets were directly seeded by FACS in the corresponding plates and cultured for 10 (bulk cultures ...
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bioRxiv - Immunology 2022Quote: ... in 96-well U-bottom plates 24 hours prior to co-culture with the indicated combinations of growth/differentiation factors (10 ng/ml each; all from Miltenyi Biotec). CD34+ HSPCs isolated from the BM of xenografted mice sacrificed between weeks 1-5 after grafting were directly seeded by FACS in the 96-well plates and cultured for 7 or 14 days before quantification of lineage outputs ...
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bioRxiv - Neuroscience 2023Quote: Analyses of the cells labeled in the previous section were conducted on a MACSQuant Analyzer 10 Flow Cytometer (#130-096-343, Miltenyi Biotec). Post-sort purity was assessed by the percent of CD11b+CD45+ singlets using MACSQuantify v2.13.0 software ...
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bioRxiv - Immunology 2022Quote: ... antibodies in T cell media (RPMI containing 10% FBS, 2mM L-Glutamine, 1% Penicilin-Streptomycin) with IL-15 and IL-7 (Miltenyi Biotec, 10ng/ml each ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then labeled with Live/Dead dye and analyzed by MACSQuant Analyzer 10 Flow Cytometer (Miltenyi Biotec, San Diego, CA).
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bioRxiv - Biochemistry 2024Quote: ... Cells were washed three times with selection buffer and labeled with 10 μL streptavidin MACS microbeads (Miltenyi Biotec, 130-048-102) (sufficient for 1e7 cells) ...
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bioRxiv - Neuroscience 2023Quote: ... the suspension was counted and incubated with Anti-O4 MicroBeads at a concentration of 10 uL of beads per 1×107 cells (Miltenyi Biotec). After incubation ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were resuspended in 1 ml 10% FBS in PBS and stained with 6 μg anti-mouse Ter119-PE antibody (Miltenyi Biotec) per 108 cells for 20 minutes at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... Peritoneal macrophages were isolated from adult (8-10 weeks old) female mice using Peritoneum Macrophage Isolation Kit (130-110-434, Miltenyi Biotec). Isolated cells were cultured in complete RPMI+GlutaMAX (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... followed by a 15 min incubation with 10 µL microbeads mixture containing antibodies directed against CD140 (130-101-502, Miltenyi Biotec), NG2 (130-097-170 ...
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bioRxiv - Neuroscience 2023Quote: ... The pelleted MVs were incubated with 10 μl of Biotin-conjugated anti-CD105 (Miltenyi Biotec cat# 130-094-916, RRID: AB_10828348) followed by adding 10 μl of anti-Biotin microbeads (MiltenyiBiotec cat# 130-094-256 ...
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bioRxiv - Developmental Biology 2023Quote: ... The cells were then strained through a 50 µm cell strainer and 1 million cells in 100 µL were incubated with 10 µL of Fc receptor block (Miltenyi Biotec) for 10 minutes on ice ...
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bioRxiv - Biophysics 2023Quote: ... antibodies in T cell media (RPMI 10 % FBS, 2 mM L-Glutamine, 1 % Penicillin-Streptomycin) with IL-15 and IL-7 (Miltenyi Biotec, 10 ng/ml each ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were washed twice and resuspended in 1mL of sorting buffer (up to 10⁸ cells) prior to magnetic separation on LS columns and the MACS Separator (Miltenyi Biotec). Columns were rinsed with 0.5mL of MACS buffer prior to loading the cell suspension ...
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bioRxiv - Cancer Biology 2023Quote: ... The samples were then centrifuged for 10 min at 3000 x g in the presence of debris removal solution (Miltenyi Biotec) and 10 min at 1000 x g in cooled PBS ...
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bioRxiv - Synthetic Biology 2023Quote: ... supplemented with 10% Human AB serum (Gemini Bio Products) with the addition of 100 U/ml human IL-2 IS (Miltenyi Biotec), 1 ng/ml IL-15 (Miltenyi Biotec) ...
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bioRxiv - Immunology 2024Quote: ... Cells were then resuspended in RPMI supplemented with 10%FBS and filtered through 30 µm MACS cell strainer (MACS Filters Miltenyi Biotec). The cells were counted to determine concentration and viability using a hemocytometer after which they were subsequently fixed for chromium fixed RNA profiling.
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Novel RNA-binding protein YebC enhances translation of proline-rich amino acid stretches in bacteriabioRxiv - Molecular Biology 2024Quote: ... Then the medium was replaced by RPMI supplemented with 10% human serum type AB and 100ng/mL GM-CSF (Miltenyi Biotec). The following day ...
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bioRxiv - Immunology 2024Quote: ... lungs of infected mice were harvested and homogenized in 1 mL of minimal essential media with 10% bovine serum albumin using a gentleMACS C Tube (Miltenyi Biotec). Subsequently ...
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bioRxiv - Immunology 2024Quote: ... Pieces of approximately 1 cm2 were placed in 10 mL PBS containing 2% FBS and homogenized for 10min at room temperature using a gentleMACS dissociator (Miltenyi Biotec). Homogenized tissue was filtered through a 70 μm cell strainer and pooled ...
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bioRxiv - Immunology 2024Quote: ... Cells were resuspended in 900 µl/107 of isolation buffer containing 0.5% Bovine Serum Albumin (BSA) and 2 mM EDTA in PBS and 10 µl/1x107 of mouse anti- Ly6G MicroBeads (Miltenyi Biotec) and incubated at 4°C for 10 mins ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were washed with PBS to remove excess AO and the cell pellet was resuspended in 500 μl PBS for flow cytometry analysis with the MACSQuant Analyzer 10 (Miltenyi Biotec) flow cytometer ...
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bioRxiv - Cancer Biology 2024Quote: ... TILs were either transduced and/or expanded in complete X-Vivo15 medium supplemented with 50 ng/ml IL-15 and 10 ng/ml IL-7 (Miltenyi Biotec).49
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bioRxiv - Cancer Biology 2024Quote: Fresh spleen was collected in 3mL complete medium (CM; DMEM + 10%FBS + 1% peniciline/streptomycin) in a homogenization tube (gentleMACSTM C Tubes, Miltenyi Biotec). Spleen was dissociated using tissue dissociator gentleMACS Octo (gentleMACSTM ...
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bioRxiv - Microbiology 2022Quote: ... stained samples were diluted 10-fold in 1x PBS and cell concentrations as well as green fluorescence intensities were recorded on a MACSQuant Analyzer 10 (Miltenyi Biotec, Germany).
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bioRxiv - Microbiology 2023Quote: Parasites were enriched from ∼100 mL of culture containing synchronized schizonts (∼36-44 h post-invasion) at ∼10% parasitemia using a SuperMACS™ II Separator and a D Column (Miltenyi Biotec). Numbers of eluted cells were determined with a hemocytometer and parasitemia was calculated from a Giemsa-stained smear ...
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bioRxiv - Cancer Biology 2023Quote: Human AML and CB cells were seeded for 10-12 days in methylcellulose-based semi-solid medium (StemMACS HSC-CFU Media complete with EPO, Miltenyi Biotec, Germany) for assessment of the frequency of colony formation ...
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bioRxiv - Cell Biology 2022Quote: BAL samples from silica beads and silica treated mice were treated for 10 min with red blood cell lysis solution (Miltenyi Biotec, 170080033) and washed with PBS by centrifugation at 300 g for 10 min ...
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bioRxiv - Cancer Biology 2023Quote: Splenic B cells from mice immunized with SRBC for 10 days were pre-enriched by B220 MicroBeads (Miltenyi Biotec; 130-049-501) followed by flow sorting ...
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bioRxiv - Immunology 2024Quote: ... Cells were incubated at 37°C for 30 minutes in the presence or absence of 10 ng/ml human recombinant IL-6 (Miltenyi Biotec, Germany) or IL-23 (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2024Quote: ... 48h or 72h after platelet addition the number of PI - positive cells was evaluated by a MACSQuant® Analyzer 10 (Miltenyi Biotec). In indicated experiments ...
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bioRxiv - Neuroscience 2020Quote: ... and homogenized mechanically into respective Gentle MACS C tubes containing 50 µL enzyme P + 1900 µL buffer Z and 20 µL of Buffer Y + 10 µL of Enzyme A (Miltenyi Biotec, Auburn, CA). Samples were passed through a 70 µm cell strainer (Millipore ...
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bioRxiv - Neuroscience 2022Quote: ... Red Blood Cell Remover Solution was made by mixing 125 μL of Red Blood Cell Lysis Solution 10× (Miltenyi Biotec, # 130-094-183) with 1125 μL of H2O ...
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Autocrine Sfrp1 inhibits lung fibroblast invasion during transition to injury induced myofibroblastsbioRxiv - Cell Biology 2022Quote: ... the labeled cells were incubated with 100 µl per 10 million cells of MACS dead cell removal beads (Miltenyi Biotec, 130-090-101) and 20 µl of each microbeads specific against AlexaFluor647 (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: The left lung was weighed and homogenized in 1 mL of RPMI supplemented with 10% FBS in GentleMACS™ M tube using GentleMACS™ Dissociator (Miltenyi Biotec) with RNA 2.01 program setting ...
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bioRxiv - Molecular Biology 2024Quote: ... The suspension was then homogenized in a Potter (10 strokes) and subsequently in a Dounce (tight pestle, 15 strokes) homogenizers and filtered through 70 µm MACS SmartStrainers (Miltenyi Biotec, United States). The homogenate was cross-linked with 1% formaldehyde for 15 min at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... in 200 μl of myeloid medium (MM) (DMEM with high glucose and L-glutamine, 10% FBS, and 1% Pen-Strep, supplemented with 50ng/ml Tpo (Miltenyi Biotec, Auburn, CA), 5ng/ml SCF ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatant was removed and the SVF resuspended in 10 ml room temperature plain DMEM followed by additional filtering through a 30 μm mesh filter (Miltenyi Biotec # 130-041-407) and subsequent centrifugation at 700 g for 5 min ...