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Citations for Miltenyi Biotec :
401 - 450 of 2586 citations for Vertical Electrophoresis Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: CD8 T cells were purified from PBMC by negative immunomagentic sorting using CD8+ T Cell Isolation Kit (Miltenyi Biotec) and used for bilayers experiments or for further separation into the subsets ...
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bioRxiv - Immunology 2022Quote: ... Mouse hepatic parenchymal cells were digested and non-parenchymal cells were isolated by Mouse Liver Dissociation Kit (Miltenyi Biotec). Isolated cells passed through 40-um cell strainer were treated with Red Blood Cell Lysis Solution (Miltenyi Biotec ...
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bioRxiv - Immunology 2019Quote: ... Treg cells were further separated with Tcon cells by using anti-PE beads (Miltenyi Biotec, catalog 130-048-801). DCs were isolated from Ly5.1+ mice by using CD11c isolation kit (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... Primary human CD8+ T cells were negatively isolated from PBMC using Human CD8+ T Cell isolation Kits (Miltenyi Biotec). Human CD8+ T cells were stimulated with CD3/CD28 activator beads (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Single-cell suspensions were sorted on the MidiMACS Quadro magnetic-activated cell separator system (Miltenyi Biotec, Bergisch Gladbach, Germany) according to manufactures instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Mouse CAFs were separated from human pancreatic UM2 cancer cells using the Mouse Cell Depletion Kit (MACS Miltenyi Biotec) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: ... then cells in a single-cell suspension were magnetically labeled with MACS CD11b microbeads (#130-093-634, Miltenyi Biotec) and were separated using MS columns (#130-042-201 ...
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bioRxiv - Genetics 2022Quote: ... Peripheral T- and B-cells were isolated from whole spleen using Pan T Cell (Miltenyi Biotec,130-095-130) or Pan B Cell (Miltenyi Biotec ...
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bioRxiv - Microbiology 2020Quote: Human primary CD4+ T cells are obtained from peripheral blood with CD4 T cell isolation kit II (Miltenyi Biotec). Unactivated CD4+ T cells were maintained in complete RPMI medium containing 50 U/ml interleukin 2 (IL-2 ...
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bioRxiv - Neuroscience 2020Quote: ... single cell iPSCs were seeded at 3×105 cells/cm2 in StemMAC iPS-Brew (Miltenyi Biotec, Bergisch Gladbach, DE) in presence of 10 µM Rock-Inhibitor Y-27632 (Cell Guidance Systems ...
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bioRxiv - Immunology 2019Quote: ... Enrichment of Lin− cells was performed prior to cell sorting by negative magnetic selection using MidiMACS Separator (Miltenyi Biotec). Cells were incubated with a biotin mouse lineage panel (Biotin-Conjugated Mouse Lineage Panel ...
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bioRxiv - Cancer Biology 2021Quote: ... The lineage negative cells were collected and then positively selected for CD117+ (cKit+) cells using CD117 microbead (Miltenyi Biotec) to yield Lin−cKit+ HSPCs ...
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The transcription factor RUNX2 drives the generation of human NK cells and promotes tissue residencybioRxiv - Immunology 2022Quote: CD34+ haematopoietic progenitor cells (HPC) were isolated by magnetic-activated cell sorting using the CD34 MicroBead kit (Miltenyi Biotec) and cultured for 16 hours in preculture medium ...
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bioRxiv - Immunology 2022Quote: ... Mouse CD4+ T cell isolation kit and CD8+ T cell isolation kit were obtained from Miltenyi Biotec (Auburn, CA). Clodronate and control liposomes were obtained from Liposoma (Amsterdam ...
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bioRxiv - Immunology 2022Quote: CD4 T cells were enriched from PBMCs by negative immunomagnetic selection using CD4+ T cell isolation kit (Miltenyi Biotec), according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Dead cells were removed from the digested PDX using a Dead Cell Removal Kit (130-090-101, Miltenyi Biotec). Murine cells were removed from the dead cell depleted fraction using a Mouse Cell Depletion Kit (130-104-694 ...
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bioRxiv - Immunology 2019Quote: ... cells were resuspended in PBS/BSA prior to depletion of CD15+ neutrophils by magnetic cell separation (MACS, Miltenyi Biotec).
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bioRxiv - Immunology 2019Quote: Single cell suspensions of PBMCs and CD15-depleted SF cells were blocked with human FcR blocking reagent (Miltenyi Biotec) and labeled for 15 min at 4 °C with antibodies for fluorescent activated cell sorting (FACS ...
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bioRxiv - Immunology 2019Quote: ... T cells were isolated using the Pan T Cell isolation kit II according to the manufacturer’s instructions (Miltenyi Biotec). Cells were cultured in RPMI 1640 (Gibco ...
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bioRxiv - Cancer Biology 2021Quote: ... the YUMM1.7-reactive cells were isolated from mouse splenocytes using mouse CD8a+ T Cell Isolation Kit (Miltenyi Biotec, Germany), resuspended at 106 cells/ml ...
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bioRxiv - Immunology 2019Quote: ... An antibody cocktail designed for negative selection of purified naïve T cells (Naïve Pan T cell purification kit, Miltenyi Biotec) was used together with extra αCD15/CD14 beads ...
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bioRxiv - Cell Biology 2020Quote: ... the cell pellet re-suspended in 20 μl of anti-PE magnetic-activated cell sorting (MACS) microbeads (Miltenyi Biotec) with 80 μL separation buffer and incubated in the dark at 4°C for 30 min ...
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bioRxiv - Cancer Biology 2020Quote: ... The lineage negative cells were collected and then positively selected for CD117+ (cKit+) cells using CD117 microbead (Miltenyi Biotec) to yield Lin-cKit+ HSPCs.
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bioRxiv - Cancer Biology 2020Quote: ... primary B cells from PBMCs using B Cell Isolation Kit II (Miltenyi Biotec, >90% purity by anti-CD19 staining). PBMCs were rested and stimulated with 100 ng/ml LPS (from E ...
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bioRxiv - Cancer Biology 2021Quote: ... The naïve T cells were purified from human PBMC using Pan T cell isolation kit (Miltenyi Biotec, Cat#130096535) and activated and expanded by CD3/CD28 Dynabeads (GibcoTM ...
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bioRxiv - Immunology 2019Quote: ... Either PBMC or B cells magnetically sorted using the AutoMACS Pro cell separator and CD19+ microbeads (both Miltenyi Biotec), were lysed in RLT buffer (Qiagen) ...
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bioRxiv - Immunology 2020Quote: ... Dermal cells were enriched for CD45-expressing cells using CD45 magnetic bead separation (Miltenyi Biotec,San Diego, CA, USA). Cell suspensions were then counted and/or labelled for flow cytometric phenotyping of surface expression markers or for flow sorting ...
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bioRxiv - Bioengineering 2021Quote: The GFP-positive cells in the retinal explant cell suspensions were enriched using a MACS Tyto sorter (Miltenyi Biotec). GFP-positive cells were resuspended in D-PBS with 0.1% BSA and processed immediately for scRNA-seq.
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bioRxiv - Immunology 2020Quote: ... cells were isolated from WT C57BL/6 spleens using a mouse naïve CD4+ T cell isolation kit (Miltenyi Biotec) and cultured as described previously (Shakya et al. ...
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bioRxiv - Immunology 2021Quote: ... B cells were first isolated by magnetic cell sorting using the human CD19 MicroBeads (Miltenyi Biotec, San Diego, CA) and the AutoMACS magnetic cell separator ...
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bioRxiv - Molecular Biology 2022Quote: ... 1×107 cells per replicate were purified using 100ul of Pluripotent Stem Cell microbeads (Miltenyi Biotec 130-095-804), and LS columns (Miltenyi Biotec 130-042-401) ...
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bioRxiv - Immunology 2023Quote: ... T cells were purified from PBMC by magnetic-bead-based negative T-cell isolation (Miltenyi Biotec, Bergisch Gladbach, Germany) as described before (Wabnitz et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... Dead cells were removed from the digested PDX using a dead cell removal kit (130-090-101, Miltenyi Biotec). Murine cells were removed from the dead-cell-depleted fraction using a mouse cell depletion kit (130-104-694 ...
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bioRxiv - Cancer Biology 2023Quote: Primary human inv(16) AML CD34+ cells were T-cell depleted using CD3 Microbeads (Miltenyi Biotec, #130-050-101) and nucleofected with the RNP complex ...
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bioRxiv - Immunology 2023Quote: ... Cells were resuspended in PBS/FCS and naïve CD4+ T cells were isolated using the naïve CD4+ T cell isolation kit (mouse) from Miltenyi Biotec according to their standard protocol ...
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bioRxiv - Genomics 2023Quote: ... and the cell pellet was resuspended in magnetic-activated cell sorting (MACS) buffer (Miltenyi Biotec No. 130-091-222). The tumor cells were purified on MACS columns in MACS buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were lineage depleted on an autoMACS using the Direct Lineage Cell Depletion Kit (Miltenyi Biotec, 130-110-470) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... Red blood cells (RBC) were lysed using Red Blood Cell Lysis Solution (catalog no. 130-094-183, Miltenyi BIotec) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... The resulting cell suspension was washed twice with PBS and used for the isolation of naïve CD4+ T cells according to the manufacturer’s instructions (Naive T Cell Isolation Kit II; Miltenyi Biotec). Naive CD4+ T cell purity was assessed by staining for CD4 PerCPeFluor710 (clone ...
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bioRxiv - Immunology 2024Quote: ZFP36L2CKO and WT OT-I T cells were purified with the MACS CD8a+ T cell isolation kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... cell isolation was performed using a magnetic cell sorting (MACS) method following the manufacturer’s instructions (Miltenyi Biotec, The Netherlands). Isolation of specific cell types was performed separately in male and female cultures An anti-CD11b microbeads antibody (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary NK cells were extracted from PBMCs by negative immunomagnetic isolation using a NK cell isolation kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... Separation of mouse cells from human tumor cells was achieved using an LS column on a MACS Separator (Miltenyi Biotec). The flow-through fraction ...
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bioRxiv - Cell Biology 2020Quote: ... WBM was depleted of terminally differentiated hematopoietic cells using a murine Lineage Cell Depletion Kit (Miltenyi Biotec 130-090-858) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2021Quote: ... The uncollected non-myeloid (CD11b-) cells were processed with the Pan T Cell Isolation Kit (Miltenyi Biotec, 130-095-130) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: Bone marrow cells were cultured (0.5×106 cells per mL) in the presence of 20 ng/mL GM-CSF (Miltenyi Biotec) in complete RPMI medium ...
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bioRxiv - Immunology 2019Quote: ... Bone marrow cells were MACS enriched for c-Kit+ cells using CD117 MicroBeads Ultrapure (Miltenyi Biotec cat #130-091-224) according to manufacturer’s protocol.
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bioRxiv - Immunology 2019Quote: ... Cells were washed and labeled for separation by negative selection using a MACS pan-dendritic cell isolation kit (Miltenyi Biotec).
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bioRxiv - Molecular Biology 2019Quote: ... Lineage negative cells were collected by applying the Direct Lineage Cell Depletion Kit mouse (130-090-858, Miltenyi Biotec GmbH) and magnetic separation following manufacturer’s instructions.
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bioRxiv - Systems Biology 2019Quote: ... Next the pellet was incubated with 200 µL/1 × 107 total cells of dead cell removal paramagnetic microbeads (Miltenyi Biotec) and incubated for 15 min at RT ...