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Citations for Miltenyi Biotec :
401 - 450 of 1703 citations for Goat anti mouse HRP secondary antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: Mouse Cell depletion was conducted according to manufacturer’s instructions for Mouse Cell Depletion kit (Miltenyi Biotec, #130-104-694). Briefly ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were then incubated for 30 min on ice with previously titrated dilutions of primary antibodies [recombinant anti-GFAP PE-conjugated (REA335, Miltenyi Biotec, Germany): 1:100 ...
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bioRxiv - Cancer Biology 2022Quote: 24-well tissue culture-treated plates were pre-coated with 1 µg/mL anti-CD3 (OKT3) antibody (Miltenyi Biotec 130-093-387) in 1 mL PBS per well ...
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bioRxiv - Genomics 2021Quote: ... CD14+ monocytes were isolated from PBMCs by a magnetic cell separation system (MACS) using anti-human CD14 antibody attached to microbeads (Miltenyi Biotec #130050201) on an LS column (Miltenyi Biotec #130-042-401 ...
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bioRxiv - Genomics 2021Quote: ... CD4+ T cells were then isolated from CD14+ monocyte-free PBMCs using anti-human CD4 antibody attached to microbeads (Miltenyi Biotec #130045101) on a new LS column ...
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bioRxiv - Immunology 2024Quote: ... seeded into well culture plates and stimulated with antigens for 18 h in presence of 1 mg/ml of anti-CD40 blocking antibody (both Miltenyi Biotec, Germany) and costimulatory molecules (CD28/CD49d ...
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bioRxiv - Immunology 2022Quote: TILs were stimulated for 7 days with anti-biotin MACSiBead Particles loaded with biotinylated CD3/CD28 antibodies (Miltenyi Biotec, Bergisch Gladbach, Germany). The preparation of the conjugated beads was carried out according to the manufacture’s recommendation (activation/expansion kit ...
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bioRxiv - Neuroscience 2024Quote: ... In most cases microglial cells were removed using a mini MACS separator based on an anti-rat CD11b/c antibody conjugated to magnetic microbeads according to manufacturer instructions (Miltenyi Biotec, CA) (Marek et al. ...
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bioRxiv - Biophysics 2024Quote: ... antibody CD56-APC (cat. 130-113-310) and antibody CD16-FITC (cat. 130-091-244) antibodies (all from Miltenyi Biotec). Cells were stored in RPMI 1640 medium (Gibco ...
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bioRxiv - Immunology 2021Quote: CD4+ cells were isolated from C7 Tg mouse spleens using mouse CD4 (L3T4) MicroBeads from Miltenyi Biotec (130-117-043) according to the manufacturer’s protocol and cultured in T cell media at 2 x 106 cells/mL with 1 μM ESAT-6 peptide pool (NIH BEI Resources Repository catalog no ...
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bioRxiv - Cancer Biology 2022Quote: ... Single mouse tumor cell suspensions were obtained using a mouse Tumor Disassociation Kit from Miltenyi Biotec (Cat# 130-096-730). After leukocytes extraction using density gradient medium ...
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bioRxiv - Systems Biology 2021Quote: ... these mice were euthanized and the YUMM1.7-reactive cells were isolated from mouse splenocytes using mouse CD8a+ T Cell Isolation Kit (130-104-075, Miltenyi Biotec), resuspended in a concentration of 106/ml ...
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bioRxiv - Cancer Biology 2023Quote: Tumor samples harvested from mouse mammary glands were dissociated using the Mouse Tumor Dissociation Kit (Miltenyi Biotec,130-096-730), gentleMACS C tubes (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... pan splenic T cells were isolated from crushed spleens of naïve C57BL/6 mouse using the Mouse Pan T Cell Isolation Kit (Miltenyi Biotec). In the subsequent co-culture incubation ...
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bioRxiv - Cancer Biology 2024Quote: ... hSS2 mouse tumors were harvested and digested to single cell suspension with the Miltenyi mouse tumor dissociation kit (Miltenyi Biotec). Approximately 5-10 million cells were crosslinked with 1% of formaldehyde ...
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bioRxiv - Immunology 2021Quote: ... Mouse FRCs required sorting using MACS (Miltenyi Biotec), with anti-mouse CD45 and CD31 magnetic beads ...
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bioRxiv - Cell Biology 2021Quote: ... Mouse IgG1-APC (Order no:130092214, Miltenyi Biotec), Mouse Anti-human CD34 (CD34-APC ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... mouse and rat (Miltenyi Biotec 130-098-373) at 37 °C with gentle pipetting ...
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bioRxiv - Immunology 2022Quote: ... were minced using scissors in RPMI media containing enzyme cocktail mix from Miltenyi Biotec mouse tumor dissociation kit (Miltenyi Biotec, Auburn, CA). Tumor pieces were further digested as per manufacturer’s instructions and digested tissue was filtered through 70μm strainer to obtain a single cell suspension ...
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bioRxiv - Immunology 2021Quote: ... mouse (Miltenyi Biotec Cat No. 130-095-927) and GentleMACS Dissociator (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2023Quote: ... mouse and rat (Miltenyi Biotec, 130-107-677) was used for adult mouse brain dissociation of WT mice and contralateral hemispheres of glioblastoma mice ...
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bioRxiv - Neuroscience 2023Quote: ... mouse and rat (Miltenyi Biotec, 130-107-677) was used according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... human and mouse (Miltenyi Biotec, 130-049-601) and a MACS separation system (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2023Quote: ... mouse FcR blocker (Miltenyi Biotec 130-092-575), added to a base blocking buffer consisting of 10% normal goat serum ...
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bioRxiv - Bioengineering 2024Quote: ... and mouse (Miltenyi Biotec, cat.: 130-092-575) for 10 min at room temperature and then stained for 30 min at 4°C with the following antibody cocktail ...
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bioRxiv - Cancer Biology 2024Quote: ... mouse (Miltenyi Biotec, Catalog no. 130-110-470), while the resulting Lin- (lineage-negative ...
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bioRxiv - Neuroscience 2024Quote: ... mouse and rat (Miltenyi Biotec, 130-107-677) was used according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... and cKIT positive (Cd117 Microbeads mouse, Miltenyi Biotec) cells were isolated from the total bone marrow cells by two steps of magnetic cell sorting (Miltenyi Biotec) ...
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bioRxiv - Molecular Biology 2024Quote: ... The recombinant mouse (rm) CSF-2 (Miltenyi Biotec) was used at a final concentration of 10 ng/ml.
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bioRxiv - Immunology 2024Quote: ... together with mouse FcR blocking Reagent (Miltenyi Biotec) were incubated for 15 min in the dark ...
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bioRxiv - Immunology 2024Quote: ... mouse and AutoMACS® Pro Separator (Miltenyi Biotec), according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... mouse and rat (130-098-305, Miltenyi Biotec), according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... mouse and rat (Miltenyi Biotec, 130-098-373), according to the manufacturer’s protocol with some modifications ...
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bioRxiv - Developmental Biology 2021Quote: ... Positive selection was subsequently applied with an antibody against biotin-conjugated integrin α7-biotin and anti-biotin MicroBeads (Miltenyi Biotec, 130-090-485), followed by MS columns (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2022Quote: ... Purified stromal cells were then separated from epithelial cells using anti-CD326 (EpCAM) antibody-coated microbeads (Miltenyi Biotec Inc., San Diego, CA, USA). Next ...
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bioRxiv - Bioengineering 2022Quote: ... Non-plasma cells were labeled with a biotin-conjugated antibody cocktail combined with anti-biotin microbeads and separated using a magnetic rack according to the manufacturer’s protocol (Miltenyi Biotec, 130-092-530). Purified plasma B cells were eluted and sequentially incubated with CD138 microbeads for an additional 15 minutes at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... PBMCs were captured by using an anti-CD19 antibody conjugated to colloidal paramagnetic microbeads (B-cell isolation kit; Miltenyi Biotec, Bergisch-Gladbach, Germany) and passed through a magnetic separation column (LS ...
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bioRxiv - Cell Biology 2022Quote: ... for 15 minutes at 4°C prior to labelling using an anti-human CD95-APC antibody (clone DX2, Miltenyi Biotec, 130-117-701) or corresponding IgG1-APC isotype (clone IS5-21F5 ...
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bioRxiv - Neuroscience 2022Quote: ... pellet was resuspended in 100 μl of PBS containing the appropriate antibodies: anti-ACSA-2-APC (1:50, Miltenyi Biotec, 130-117-535) and anti-GLUT1 conjugated to Alexa Fluor 488TM (1:200 ...
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bioRxiv - Bioengineering 2024Quote: ... were obtained after transduction of Jurkat T cells with a serial dilution of viral supernatants and transduction efficiency evaluated after 3-4 days by monitoring cell surface dNGFR expression using an anti-CD271-APC antibody (Miltenyi Biotec., Paris, France) or HLA-A*02 Dextramer-APC (Immudex ...
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bioRxiv - Molecular Biology 2024Quote: ... Isolation of specific cell types was performed separately in male and female cultures An anti-CD11b microbeads antibody (Miltenyi Biotec, 130-049-601) and an anti-O4 microbeads antibody were used to isolate microglia and oligodendrocytes ...
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bioRxiv - Immunology 2021Quote: ... Conventional T cells were enriched from FVB/N mouse spleen single-cell suspensions using the mouse Pan T Cell Isolation Kit II (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... purified CD8+ T-cells were coated with mouse IL-2 catch reagent from a mouse IL-2 secretion assay detection kit (Miltenyi Biotec) by incubating T cells in a 20x dilution of IL-2 catch reagent in R10 medium for 15 min on ice ...
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bioRxiv - Developmental Biology 2023Quote: ... red blood cell lysis was performed with ACK buffer (Thermo) before mouse cells were removed from the dissociated lungs using the mouse cell depletion kit (Miltenyi Biotec) yielding purified MeWo cells that achieved successful lung colonization ...
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bioRxiv - Immunology 2023Quote: ... and the appropriate programs were run according to the mouse tumor dissociation kit protocol and mouse lung dissociation kit protocol (both from Miltenyi Biotec) for tumors and lungs respectively ...
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bioRxiv - Biochemistry 2024Quote: ... mouse cell depletion was performed on whole bone marrow cells using the Mouse Cell Depletion Kit and LS columns (Miltenyi Biotec). RIPA lysis was performed with purified human cells and 25µg of protein were loaded on 10% Bis-Tris-Gel NuPAGE gels with MOPS SDS running buffer (Life Technologies) ...
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bioRxiv - Cancer Biology 2021Quote: ... αβ T cells were transduced with pMP71: γ-T2A-δ and incubated with a biotin-labelled anti-αβ TCR antibody (clone BW242/412; Miltenyi Biotec) followed by incubation with an anti-biotin antibody coupled to magnetic beads (anti-biotin MicroBeads ...
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bioRxiv - Genomics 2020Quote: ... Cells were washed with cold PBS/10% FBS and resuspended in 10 μl of cold PBS/10% FBS per 106 cells and stained with a 1/100 dilution of anti-Ter119-PE antibody (Miltenyi Biotec: 130-102-336) at 4 °C for 20 minutes ...
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bioRxiv - Immunology 2021Quote: ... Cells were stimulated for 6 hours with 2 μg/ml of each of the 15 matrix peptide pools in the presence of 1 µg/ml anti-CD40 antibody (HB14) (purchased from Miltenyi Biotec, Bergisch Gladbach, Germany) and 1 μg/ml anti-CD28 antibody (CD28.2 ...
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bioRxiv - Immunology 2021Quote: αβT cells transduced with γδTCR-Cl5 either alone or together with NKG2D wild type or the different NKG2D chimeras were incubated with a biotin-labeled anti-αβTCR antibody (clone BW242/412; Miltenyi Biotec, Bergisch Gladbach, Germany) and subsequently incubated with an anti-biotin antibody coupled to magnetic beads (anti-biotin MicroBeads ...