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Citations for Miltenyi Biotec :
401 - 450 of 3339 citations for Cow T Cell Surface Glycoprotein CD3 Gamma Chain CD3G ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: Primary murine CD4 T cells were isolated from the spleens and lymph nodes of mice using a negative isolation kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Responder CD8 T cells were isolated from the spleens and lymph nodes of mice using a negative isolation kit (Miltenyi Biotec) and stained with 5μM CellTrace Violet (CTV ...
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bioRxiv - Immunology 2020Quote: Bulk polyclonal CD8+ T cells were isolated from the spleen and lymph nodes of WT or Dct-/- mice using negative magnetic enrichment (CD8a+ T cell Isolation Kit; Miltenyi Biotec). Enriched CD8+ T cells (typically ∼75-90% pure ...
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bioRxiv - Immunology 2020Quote: ... naïve CD4+ T-cells were isolated from lymph nodes and spleens of OT-II TCR transgenic mice using negative selection with a mouse CD4+ T Cell Isolation Kit (Miltenyi Biotec). Splenocytes from C57BL/6 mice were T cell depleted by complement lysis of Thy1.2+ cells (clone J1J ...
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bioRxiv - Biophysics 2022Quote: ... Naive T cells were purified via magnetic negative selection from spleens of 6–8 week old mice using either CD4+ (for 3.L2) or CD8+ (for P14 and OT1) T-cell isolation kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Treg and Tconv cells were enriched from PBMCs of patients with IBD and healthy donors using MACS CD4+CD25+ Regulatory T Cell Isolation Kit human (Miltenyi Biotec) for the co-culture experiments ...
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bioRxiv - Immunology 2023Quote: ... and 1:8 with CD8+ T cells enriched using mouse CD8 (Ly-2) Microbeads kit (Miltenyi Biotec Cat # 130-049-401) and labeled with CellTrace Violet (CTV ...
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bioRxiv - Immunology 2023Quote: ... purified CD8+ T-cells were coated with mouse IL-2 catch reagent from a mouse IL-2 secretion assay detection kit (Miltenyi Biotec) by incubating T cells in a 20x dilution of IL-2 catch reagent in R10 medium for 15 min on ice ...
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bioRxiv - Bioengineering 2023Quote: CD4+ and CD8+ T cells were then isolated from PBMCs by magnetic associated cell sorting (MACS) using the CD4+ or CD8+ human T cell isolation kit (130-096-533 or 130-096-495; Miltenyi Biotec), respectively ...
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bioRxiv - Immunology 2023Quote: ... or Kdm6aflox/flox LckCre (UTXTCD) 8- to 12-week-old female donor spleens using the CD4+ T cell Isolation Kit II (Miltenyi Biotec) according to manufacturer instructions ...
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bioRxiv - Immunology 2023Quote: ... Red blood cells were lysed using RBC lysis buffer before being magnetically sorted using the CD8+ T cell isolation kit (Catalog # 130-096-543, Miltenyi Biotec). Neutrophil preparation ...
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bioRxiv - Cancer Biology 2023Quote: CD4+ T cells were magnetically isolated from the spleens of healthy C57B6129SF1/J mice using a mouse CD4+ T cell isolation kit (Miltenyi Biotec). The CD4+ T cells were cultured for 3 days with or without KPC-derived PDAC cells in the presence of low dose anti-CD3/CD28 beads (x 40 dilution ...
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bioRxiv - Immunology 2023Quote: ... Naïve CD4+ T cells from the sample were purified by magnetic bead separation using the CD4+CD62L+ isolation kit (Miltenyi Biotec). Cells were plated in 96-well plates coated with anti-CD3 antibody (5μg/ml ...
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bioRxiv - Microbiology 2024Quote: ... Norway) centrifugation and used for CD4+ T cell isolation by negative selection with a commercial kit (Miltenyi Biotec, Bergisch Gladbach, Germany) per manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... T cell stimulation was induced with anti-CD3ε and anti-CD28 beads (T Cell Activation/Expansion Kit mouse, Cat.#130-093-627, Miltenyi Biotec) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... were isolated from the spleen and lymph nodes of FoxP3-aPIK3CD mice using the CD4+CD25+ Regulatory T Cell Isolation Kit (Miltenyi Biotec). Genomic DNA was isolated from these cells using the AllPrep DNA/RNA Micro Kit (Qiagen) ...
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bioRxiv - Immunology 2022Quote: ... MAIT cell staining (1 ×106 PBMC) was performed using specific surface monoclonal antibodies (All Miltenyi Biotec) namely ...
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bioRxiv - Immunology 2022Quote: Splenic iNKT cells were isolated from C57BL/6 mice sorting CD45+CD3+CD1d:PBS57Tet+ cells upon enrichment through B cells exclusion (Mouse CD19 microbeads, Miltenyi Biotec). Bone marrow derived dendritic cells (BMDCs ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T cells were isolated from the CD14− cell fraction by negative selection (Miltenyi Biotec) and stained with CD4-PerCP/Cy5.5 (SK3) ...
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bioRxiv - Immunology 2023Quote: ... Cells were then positively enriched for tetramer+ T cells using a magnetic column (Miltenyi Biotec.) according to manufacturer’s instructions and washed before extracellular staining.
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bioRxiv - Cell Biology 2021Quote: Human peripheral blood lymphocytes (PBLs) from a healthy donor were stimulated using the T Cell Activation/ Expansion Kit (MACS,Miltenyi Biotec, USA) according to manufacturer instructions ...
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bioRxiv - Immunology 2022Quote: γδ T cells were sorted from the spleen of MCMV infected or control TCRα-/- mice using the TCR γδ T cell Isolation kit (Miltenyi Biotec). For γδ TCM and TEM sorting ...
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bioRxiv - Immunology 2023Quote: CD8+ T cells were isolated from RBC-depleted splenocytes using the Miltenyi CD8+ negative selection kit (Miltenyi Biotec, Cat # 130-104-075). Reporter-expressing CD8+ T cells were sorted using a BD Aria III ...
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bioRxiv - Immunology 2023Quote: ... naïve 8.3 CD8 T cells from the LNs of non-diabetic NY8.3 NOD mice were purified using two rounds of the naïve CD8 T cell isolation kit (Miltenyi Biotec, Bergisch Gladbach, Germany) with LS columns (Miltenyi Biotec) ...
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bioRxiv - Immunology 2023Quote: ... Tregs were purified from the mouse lymphocytes using a CD4+CD25+ regulatory T cell isolation kit (Miltenyi Biotec, cat. # 130-091-041). The purity of the isolated cells was checked by flow cytometry ...
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bioRxiv - Immunology 2022Quote: ... Autologous naïve T cells and NK cells were purified by magnetic-activated cell sorting (MACS®; Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2021Quote: ... Surface staining with antimouse CD8/APC (Miltenyi Biotec), anti-mouse CD4/APC-Cy7 (BD Biosciences) ...
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bioRxiv - Immunology 2019Quote: ... Purified CD4+ T-cells were stained with CD4 antibody (Biotin, Miltenyi Biotec) and CD25 antibody (PE ...
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bioRxiv - Immunology 2020Quote: Naïve splenic CD4+ T cells were purified by magnetic separation (Miltenyi Biotec). T cells were activated by α-CD28 (5 ug/ml ...
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bioRxiv - Immunology 2020Quote: CD4+ T cells were purified using anti-CD4+ conjugated microbeads (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... and CD4+ T PbTII cells were enriched using CD4 microbeads (Miltenyi Biotec). Cells (104/200 µl ...
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bioRxiv - Bioengineering 2021Quote: ... we suspended pelleted PBMCs in the sorting buffer (80 μL for 107 cells) with CD3 magnetic microbeads (20 μL for 107 cells) from Miltenyi Biotec for 15 min at 4 °C according to the capacity of the microbeads ...
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bioRxiv - Microbiology 2021Quote: ... CD2/CD3/CD28 activation beads (Miltenyi Biotec, Germany) or plastic-adhered anti-CD3 mAb plus soluble anti-CD28 mAb for 2-3 days ...
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bioRxiv - Immunology 2022Quote: ... CD3−sorted cells were collected and stained with anti-NKp44-PE conjugated to ferromagnetic microbeads (Miltenyi Biotec) and directed through a cell separation column containing a magnetic field (Keren et al ...
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bioRxiv - Immunology 2019Quote: Splenic CD4+ T cells or CD19+ B cells were positively selected using immunomagnetic MicroBeads (Miltenyi Biotec) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: CD8+ T-cells were isolated from HLA.A2- donors using magnetic-activated cell sorting (MACS, Miltenyi Biotec). Anti-CD3/CD28/CD2 Immunocult (Stemcell Technologies ...
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bioRxiv - Immunology 2020Quote: ... and the complete sequence of the nucleocapsid phosphoprotein N and the membrane glycoprotein M (GenBank MN908947.3, Protein QHD43416.1, Protein QHD43423.2, Protein QHD43419.1) (Miltenyi Biotec, Germany). After 5 hours of stimulation ...
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bioRxiv - Immunology 2019Quote: ... Mouse total T and CD8+ native T cells were enriched from spleens and lymph nodes by negative selection using MACS system (Miltenyi Biotec, Auburn, CA) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... CD8 T cells from spleens and lymph nodes of either P14 or FSF-Tim-3/E8i-Cre/P14 mice were purified by magnetic separation using a naïve CD8+ T cell isolation kit (Miltenyi Biotec #130-104-075) and then mixed with gp33-pulsed B cells at a ratio of 1:1 and incubated for indicated times at 37°C.
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bioRxiv - Immunology 2021Quote: ... were isolated from the spleen of naive Rdh10f/f Cd4Cre Rag1-/- OT-I CD45.1+ CD45.2+ (Rdh10CKO) or Rdh10f/f Rag1-/- OT-I CD45.1+ (control) mice using the Pan T Cell Isolation Kit II (Miltenyi Biotec, Bergisch Gladbach, Germany). For the adoptive transfer of memory T cells ...
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bioRxiv - Cancer Biology 2023Quote: CD3+ T cells were enriched from the BM of 30 MM patients using the Pan T cell isolation kit with MS columns (both Miltenyi Biotec, Bergisch, Germany). 5 x 105 CD3+ T cells were plated in 0.5 ml T cell expansion medium (Stemcell Technologies ...
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bioRxiv - Immunology 2021Quote: ... On day4 transduced cells were positively selected by magnetic assisted cell sorting (MACS) via the cell surface antigen CD90.1 (Thy1.1) using microbeads (Miltenyi Biotec: 130-094-523); the manufacturer’s protocol was adjusted by using 4x less reagents and by maintaining cells at room temperature ...
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bioRxiv - Immunology 2019Quote: ... CD4+ T cells were enriched using CD4 microbeads (Miltenyi Biotec; Bergisch Gladbach, Germany).
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bioRxiv - Immunology 2019Quote: ... with CD4 T cells depleted using CD4+ magnetic beads (Miltenyi Biotec, Auburn, CA), and then irradiated with 3000 rads ...
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bioRxiv - Immunology 2019Quote: ... The T cell isolation was done according to the manufacturer’s protocol (Miltenyi Biotec), however prolonging the incubation with CD3ε-Biotin to 20 min ...
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bioRxiv - Immunology 2020Quote: ... CD8+ T cells were purified from PBMC via human CD8 MicroBeads (Miltenyi Biotec) and activated with T Cell TransAct (Miltenyi Biotec) ...
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bioRxiv - Immunology 2021Quote: ... CD4+ T cells were enriched using microbeads (Miltenyi Biotec, Cat. #.: 130-117-043). Naïve CD4+ T cells (CD4+CD44lowCD62LhighCD25− ...
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bioRxiv - Immunology 2021Quote: ... T cells were selected using human CD4 microbeads and MS columns (Miltenyi Biotec). Procedure was carried out according to manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... 1G4 T cells were isolated using “untouched isolation” MACS microbead selection (Miltenyi Biotec). Cells were labeled labelled with CTV by mixing 1:1 in PBS ...
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bioRxiv - Immunology 2023Quote: ... and CD4+ T cells were enriched using MACS CD4 (L3T4) microbeads (Miltenyi Biotec). Next ...