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Citations for Miltenyi Biotec :
351 - 400 of 441 citations for Ubiquitin thioesterase ZRANB1 ZRANB1 Antibody HRP since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... at a final concentration of 1 µg/mL in the presence of a blocking CD40 antibody (1 µg/mL; Miltenyi Biotec) and CD28 antibody (1 µg/mL ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were stained with APC-efluo780 conjugated anti-c-kit antibody and c-kit+ cells were enriched using anti-APC paramagnetic microbeads (Miltenyi Biotec). Cells were stained with fluorophore-conjugated antibodies against lineage markers (CD2 ...
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bioRxiv - Microbiology 2023Quote: ... and 20 minute incubation with primary antibody cocktail (1:50 anti-CD45.1-PE, REAfinity [Miltenyi Biotec, Germany]/1:50 anti-CD45.2-VioBlue, REAfinity [Miltenyi Biotec, Germany]). Samples were analyzed using a S1000 Flow Cytometer with an A600 96-well plate high-throughput extension (Stratedigm ...
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bioRxiv - Cell Biology 2022Quote: ... Magnetic enrichment was carried out on an AutoMACS using microbeads conjugated to antibodies recognizing biotin or mouse CD117 (all from Miltenyi Biotec). HSCs (CD45.2+ ...
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bioRxiv - Bioengineering 2023Quote: ... T cells were enriched by MACS using an in-house biotinylated anti-EGFR antibody (Cetuximab, Eli Lilly and Company, Indianapolis, IN, USA) and anti-Biotin Microbeads (Miltenyi Biotec). Purity of enriched CAR modified T cells was analyzed as described above ...
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bioRxiv - Cancer Biology 2022Quote: ... standard CAR-T cells for the clinical setting were produced except for several modifications(20): CD8+ T cells were stimulated with 1 ug/ml OKT-3/CD28 antibodies (Miltenyi Biotec) for 72 h ...
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bioRxiv - Immunology 2023Quote: ... Cells were depleted of CD4+ T cells by staining with CD4 PE-conjugated antibody (RM4-4) for 20 minutes at 4°C and then with Anti-PE MicroBeads (Miltenyi Biotec.) as described above ...
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bioRxiv - Immunology 2023Quote: ... CD11c-biotin and B220-biotin antibodies for 20 min at 4°C and labeled with anti-biotin magnetic beads (Miltenyi Biotec) for 15 min at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... and then co-stained for 15 min at 4 °C with PE-conjugated anti-astrocyte cell surface antigen-2 (ACSA-2) antibody (130-116-244; Miltenyi Biotec) and Alexa Fluor 647-conjugated anti-O1 antibody (FAB1327R ...
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bioRxiv - Molecular Biology 2023Quote: ... Negative controls were incubated with fluorochrome-labeled irrelevant isotype control antibody (REA Control APC, mouse cod. 130-113-446 Miltenyi Biotec).
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bioRxiv - Immunology 2023Quote: ... cells were washed twice with FACS Intracellular Staining Perm Wash buffer and the following antibodies were added to the cells: anti-STAT1 pTyr701 (Miltenyi Biotec), and anti-STAT3 pTyr705 (eBioscience™) ...
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bioRxiv - Molecular Biology 2023Quote: ... natural killer cells and monocytes were isolated from PBMCs by negative selection and magnetic sorting using cocktails of biotin-conjugated antibodies against non-target cells (#130-096-535,Miltenyi Biotec for T cells ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were resuspended in 1 ml 10% FBS in PBS and stained with 6 μg anti-mouse Ter119-PE antibody (Miltenyi Biotec) per 108 cells for 20 minutes at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... Red blood cell lysed splenocytes were labeled with microbeads conjugated to monoclonal anti-mouse CD19 antibodies (Miltenyi Biotec, Bergisch Gladbach, Germany) and positively sorted as detailed by the manufacturer ...
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bioRxiv - Cell Biology 2023Quote: The gastrocnemius muscles were minced and enzymatically digested as explained above and cells were magnetically labeled using anti-F4/80 antibody and isolated by magnetic sorting (Miltenyi Biotec).
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bioRxiv - Biochemistry 2023Quote: ... and the flowthrough was enriched for CD4+ T cells by positive selection using anti-CD4 microbead antibodies (cat#130-045-101, Miltenyi Biotec). CD4+ T cell enrichment was confirmed by determining the percentage of CD3+/CD4+ cells via flow cytometry ...
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bioRxiv - Cancer Biology 2023Quote: ... human T-ALL cells were purified after labeling with anti-huCD45 PE conjugated antibody followed by immuno magnetic selection with anti-Phycoerythrin (PE) microbeads (130-048-801, Miltenyi Biotec).
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bioRxiv - Immunology 2024Quote: ... stimulated with IL-2 (R&D), and/or T cell differentiating cytokines and antibodies (Th1: anti-IL-4, IL-12 (Miltenyi Biotec), Treg ...
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bioRxiv - Cell Biology 2024Quote: ... 100 μL of each sample was removed and set aside as representative “crude mitochondrial fraction.” The remaining 300 μL was diluted to 2 mL total with ICB and combined with 60 μL of superparamagnetic microbeads coated with mouse IgG1 antibodies against GFP (#130-091-125; Miltenyi Biotec). These samples were then placed on a rotating wheel at +4°C for 1 hr ...
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bioRxiv - Immunology 2024Quote: ... Surface staining was performed for 15 min in the presence of 1 mg/ml of Beriglobin (CSL Behring) with the following fluorochrome-conjugated antibodies titrated to their optimal concentrations: anti-CD3-FITC (Miltenyi Biotec), anti-CD4-VioGreen (Miltenyi Biotec) ...
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bioRxiv - Immunology 2024Quote: ... a 24-well plate was pre-coated with anti-CD3 (OKT3) and anti-CD28 antibodies (15E8) (Miltenyi Biotec, Macquarie Park, Australia) at 1µg mL-1 on day 0 ...
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bioRxiv - Neuroscience 2024Quote: ... day 27 cultures were first enriched for motor neurons by using antibodies against PSA-NCAM and L1CAM (both from Miltenyi Biotec) [39] ...
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bioRxiv - Cell Biology 2024Quote: ... proteins from the native gel were transferred to the PVDF membrane and probed with anti-CD95 antibody (Miltenyi Biotec, clone DX2).
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bioRxiv - Cell Biology 2024Quote: ... The bead-EV complexes were resuspended in 100 µL/sample of filtered PBS and incubated with the following conjugated monoclonal antibodies from Miltenyi Biotec: mouse anti-CD9-APC antibody (Clone REA1071) ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were then incubated for 30 min on ice with previously titrated dilutions of primary antibodies [recombinant anti-GFAP PE-conjugated (REA335, Miltenyi Biotec, Germany): 1:100 ...
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bioRxiv - Synthetic Biology 2020Quote: ... Dynabead-EV samples were re-suspended into 98 μl DPBS and 2 μl of one of the following antibodies: IgG1-PE (#130-113-200, Miltenyi Biotec, Germany), CD9-PE ...
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bioRxiv - Bioengineering 2022Quote: ... PhDF were analyzed by flow cytometry using CD90 antibody conjugated to APC-Vio770 (CD90; 1:50; REA897; 130-114-905, Miltenyi Biotec, Germany).
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bioRxiv - Cancer Biology 2022Quote: 24-well tissue culture-treated plates were pre-coated with 1 µg/mL anti-CD3 (OKT3) antibody (Miltenyi Biotec 130-093-387) in 1 mL PBS per well ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then stained with a CD34-PE-Vio770 antibody (20 minutes at 4°C; Miltenyi Biotec, clone AC136, #130-113-180) and DAPI (Invitrogen ...
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bioRxiv - Genomics 2021Quote: ... CD14+ monocytes were isolated from PBMCs by a magnetic cell separation system (MACS) using anti-human CD14 antibody attached to microbeads (Miltenyi Biotec #130050201) on an LS column (Miltenyi Biotec #130-042-401 ...
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bioRxiv - Genomics 2021Quote: ... CD4+ T cells were then isolated from CD14+ monocyte-free PBMCs using anti-human CD4 antibody attached to microbeads (Miltenyi Biotec #130045101) on a new LS column ...
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bioRxiv - Immunology 2022Quote: TILs were stimulated for 7 days with anti-biotin MACSiBead Particles loaded with biotinylated CD3/CD28 antibodies (Miltenyi Biotec, Bergisch Gladbach, Germany). The preparation of the conjugated beads was carried out according to the manufacture’s recommendation (activation/expansion kit ...
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bioRxiv - Immunology 2024Quote: ... seeded into well culture plates and stimulated with antigens for 18 h in presence of 1 mg/ml of anti-CD40 blocking antibody (both Miltenyi Biotec, Germany) and costimulatory molecules (CD28/CD49d ...
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bioRxiv - Molecular Biology 2023Quote: ... natural killer cells and monocytes were isolated from PBMCs by negative selection and magnetic sorting using cocktails of biotin-conjugated antibodies against non-target cells (#130-096-535,Miltenyi Biotec for T cells, (#130-101-638, Miltenyi Biotec for B cells ...
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bioRxiv - Immunology 2023Quote: ... were incubated in 96-well flat-bottom plate and stimulated at a ratio of 2:1 in the presence of NHP-specific MACSiBead™ Particles loaded with biotinylated antibodies to mimic antigen-presenting cells (T Cell Activation/Expansion Kit, Miltenyi Biotec). Responder cells were labeled with cell trace violet prior to incubation ...
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bioRxiv - Cancer Biology 2024Quote: ... CD34+ cells from cord blood or adult donors were sorted using microbeads conjugated to monoclonal mouse anti-human CD34 antibodies (Miltenyi Biotec MACS) through positive magnetic bead selection on LS columns.
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bioRxiv - Molecular Biology 2024Quote: ... CD45+ dynabeads were then removed by placing the sample on a magnetic stand and the remaining cells (CD45-) were incubated in CD140b-APC antibody (Miltenyi Biotec, Germany) for 30 minutes at 4°C on a shaker ...
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bioRxiv - Neuroscience 2024Quote: ... In most cases microglial cells were removed using a mini MACS separator based on an anti-rat CD11b/c antibody conjugated to magnetic microbeads according to manufacturer instructions (Miltenyi Biotec, CA) (Marek et al. ...
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bioRxiv - Developmental Biology 2024Quote: ... the homogenate was incubated with microbeads conjugated to a cocktail of monoclonal antibodies against no-target cells (Miltenyi Biotec, Bergisch Gladbach, Germany) at 2-8 °C for 20 min ...
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bioRxiv - Developmental Biology 2021Quote: ... Positive selection was subsequently applied with an antibody against biotin-conjugated integrin α7-biotin and anti-biotin MicroBeads (Miltenyi Biotec, 130-090-485), followed by MS columns (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2022Quote: ... Purified stromal cells were then separated from epithelial cells using anti-CD326 (EpCAM) antibody-coated microbeads (Miltenyi Biotec Inc., San Diego, CA, USA). Next ...
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bioRxiv - Neuroscience 2020Quote: ... cells dissociated as above were immunopurified with mouse monoclonal antibody to Thy-1 (BSJ-1: French and Jeffrey, 1986) using goat anti-mouse IgG conjugated magnetic beads (Miltenyi Biotec, Auburn, CA) (Yamagata et al. ...
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bioRxiv - Neuroscience 2020Quote: ... Antibody staining for FACS was performed in Flow Cytometry Staining Buffer (ThermoFischer Scientific) and following antibodies were used: PE-GLAST (ACSA-1, Miltenyi Biotec, 1/40), APC-CD133 (13A4 ...
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bioRxiv - Bioengineering 2022Quote: ... Non-plasma cells were labeled with a biotin-conjugated antibody cocktail combined with anti-biotin microbeads and separated using a magnetic rack according to the manufacturer’s protocol (Miltenyi Biotec, 130-092-530). Purified plasma B cells were eluted and sequentially incubated with CD138 microbeads for an additional 15 minutes at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... The remaining pellet was resuspended in 80 μL of 0.5% BSA and 20 μL of Non-Neuronal Cells Biotin-Antibody Cocktail (Miltenyi Biotec, # 130-115-389) and incubated for 5 minutes at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... Surface phenotype of cells was determined by flow cytometry using fluorochrome-conjugated antibodies specific for CD3 (Miltenyi Biotec, Inc. Cat#130-094-363), CD19 and CD107a (BD Biosciences Cat#555800) ...
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bioRxiv - Cancer Biology 2021Quote: ... PBMCs were captured by using an anti-CD19 antibody conjugated to colloidal paramagnetic microbeads (B-cell isolation kit; Miltenyi Biotec, Bergisch-Gladbach, Germany) and passed through a magnetic separation column (LS ...
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bioRxiv - Immunology 2020Quote: Monocytes were purified from freshly thawed PBMC using CD14 antibodies conjugated to magnetic microbeads per the manufacturer’s recommendations (Miltenyi Biotec, San Diego CA). Magnetically bound monocytes were washed and eluted for collection ...
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bioRxiv - Neuroscience 2022Quote: ... pellet was resuspended in 100 μl of PBS containing the appropriate antibodies: anti-ACSA-2-APC (1:50, Miltenyi Biotec, 130-117-535) and anti-GLUT1 conjugated to Alexa Fluor 488TM (1:200 ...
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bioRxiv - Molecular Biology 2024Quote: ... Isolation of specific cell types was performed separately in male and female cultures An anti-CD11b microbeads antibody (Miltenyi Biotec, 130-049-601) and an anti-O4 microbeads antibody were used to isolate microglia and oligodendrocytes ...