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Citations for Miltenyi Biotec :
351 - 400 of 1280 citations for Trisodium dichloroanthra 2 1 9 mna naphth 2 3 h acridine 5 10 15 triyl tris sulfate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Single cell transcriptomics reveals the effect of PD-L1/TGF-β blockade on the tumor microenvironmentbioRxiv - Genomics 2020Quote: ... A red blood cell lysis step was performed by incubating cells for 2 minutes with 1x Red Blood Cell Lysis Solution (Miltenyi Biotec). Cells were washed again and then cell viability was measured using the Nexcelom Cellometer K2 Fluorescent Viability Cell Counter and the ViaStain AOPI Staining Solution (Nexcelom) ...
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bioRxiv - Microbiology 2020Quote: ... Each section was weighed and then homogenized in 2 ml PBS in gentleMACS M tubes and a gentleMACS Octo Dissociator (Miltenyi Biotec) using the RNA_1 setting ...
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bioRxiv - Immunology 2020Quote: ... PBMCs from 48 healthy individuals (samples taken before the SARS-CoV-2 outbreak) were enriched for CD19+ cells with CD19-microbeads (Miltenyi Biotec). For each individual ...
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bioRxiv - Immunology 2023Quote: ... T cells were stimulated by the addition of 100 IU/mL IL-2 (Proleukin) and anti-CD3/CD28 stimulation using loaded MACSiBead particles (Miltenyi Biotec) in a proportion of 1:1 of beads to cell ...
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bioRxiv - Immunology 2023Quote: ... splenocytes were resuspended in CTL test media (Immunospot) and incubated at 37 °C for 2 hours with either no stimulation or S1 peptide simulation (Miltenyi Biotec) at a final concentration of 2 µg/mL before adding GolgiPlug to all samples for 4 additional hours ...
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bioRxiv - Immunology 2023Quote: ... Sarcoma tissue was collected on ice and immediately cut into 2 mm3 segments and dissociated to a single-cell suspension using the Human Tumor Dissociation Kit and gentleMACS dissociator (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: B cells were purified from the spleens of mice (aged 2–7 months of age) through negative selection with anti-CD43 magnetic beads (Miltenyi Biotec) and subsequently cultured in RPMI 1640 media (Life Technologies ...
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bioRxiv - Bioengineering 2023Quote: Lung cells were centrifuged and resuspended with fresh complete RPMI media with 20 μL/mL of PeptTivator® SARS-CoV-2 Prot_S (Miltenyi Biotec) and 5 μg/mL Brefeldin A (Biolegend) ...
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bioRxiv - Immunology 2024Quote: ... In-vitro CD4 T cell survival assays were performed in complete RPMI medium supplemented with recombinant murine IL-2 (100 ng/ml, Miltenyi Biotec) or IL-7 (10 ng/ml ...
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bioRxiv - Cell Biology 2024Quote: ... cells were stained with ITGA7-APC and ITGA5-PE (Supplementary Table 2) and sorted using a MACSQuant Tyto Cell Sorter (Miltenyi Biotec). Flow cytometry was performed regularly during SC proliferation using a MACSQuant10 Flow Analyzer (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... stimulated with IL-2 (R&D), and/or T cell differentiating cytokines and antibodies (Th1: anti-IL-4, IL-12 (Miltenyi Biotec), Treg ...
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bioRxiv - Neuroscience 2024Quote: ... Then the astrocytes or newborn neurons were isolated using Anti-ACSA-2 MicroBead Kit or Anti-PSA- NCAM MicroBead Kit (Miltenyi Biotec). Briefly to say ...
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bioRxiv - Immunology 2021Quote: ... Fc receptors were blocked by incubation for 15 minutes in Fc Blocking Reagent (1:100 in FACS buffer, Miltenyi Biotec, Bergisch Gladbach, Germany). Single-cell suspensions were stained with the indicated fluorescent antibodies on ice for 45 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 × 105 cells were stained using CD117-APC (Miltenyi Biotec, 130-098-207, 1:50) and CD133-PE (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... Add diluted 7-AAD (1:100 in the FACS buffer) to exclude dead cells and followed by Fc-receptor block (1:5 dilution, Miltenyi Biotec) to block non-specific staining ...
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bioRxiv - Cancer Biology 2023Quote: ... PE anti-human MSCA1 (Clone W8B2, dilution 1/10, Miltenyi Biotec, Paris, France), APC antihuman CD271 (Clone ME20.4-1.H4 ...
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bioRxiv - Cancer Biology 2024Quote: ... stained cells were washed 3 times with MACS Buffer (1:20 in PBS, Miltenyi Biotec, 130-091-376) to remove any remaining unbound antibody ...
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bioRxiv - Immunology 2022Quote: ... Cells were stimulated with 25 ng/ml IL-15 (Miltenyi Biotec) for 6 hours and ADCC effector cells were enriched from PBMCs by depletion of T cells using anti-CD3 coupled magnetic beads from the EasySep™ Human T Cell Isolation Kit (Stemcell) ...
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Effective targeting of CD19 positive primary B-ALL cells using CAR-NK cells generated with mRNA-LNPsbioRxiv - Cancer Biology 2024Quote: ... and IL-15(10ng/ml; Miltenyi Biotec, Inc., San Diego, CA) and 10% FBS in RPMI 1640 (Biosera ...
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bioRxiv - Synthetic Biology 2020Quote: ... Dynabead-EV samples were re-suspended into 98 μl DPBS and 2 μl of one of the following antibodies: IgG1-PE (#130-113-200, Miltenyi Biotec, Germany), CD9-PE ...
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bioRxiv - Cancer Biology 2021Quote: Surgical specimens placed immediately in cold sterile DMEM after excision were cut into 2-4 mm pieces before undergoing enzymatic and mechanical dissociation using the gentleMACS™ Octo Dissociator multitissue kit and protocol (Miltenyi Biotec). Resuspended cells were then promptly plated into flasks ...
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bioRxiv - Bioengineering 2021Quote: ... Nalm6 tumors were extracted on day 4 post bacteria treatment (day 2 post T cell treatment) and lymphocytes were isolated from tumor tissue by mechanical homogenization using a gentleMACS™ Dissociator (Miltenyi Biotec) in complete hTcm ...
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bioRxiv - Microbiology 2022Quote: ... 1 ml of each sample was transferred to a 2 ml Eppendorf tube and the samples were assayed on a flow cytometer (MACSQuant® VYB, Miltenyi Biotec). Green fluorescent cells were detected using the blue laser (488 nm ...
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bioRxiv - Developmental Biology 2022Quote: ... The remaining cells were pelleted and resuspended in 80 µl of MACS buffer (PBS pH 7.2, 0.5% BSA and 2 mM EDTA) and 20 µl anti-CD45 MicroBeads (Miltenyi Biotec #130-052-301) or 20 µl anti-F4/80 MicroBeads (Miltenyi Biotec #130-110-443 ...
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bioRxiv - Biochemistry 2021Quote: ... The cell pellet was resuspended in 80 μL of 2% BSA/PBS and incubated with 20 μL of anti-PE MicroBeads UltraPure (Miltenyi Biotec, USA.) for 15 min at 4°C ...
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bioRxiv - Immunology 2021Quote: ... 2 mM EDTA in PBS) before positive selection of CD14+ monocytes by incubation with magnetic CD14 microbeads (Miltenyi Biotec, 130-050-201) (15 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Tumors were cut in small pieces of approximately 2 mm3 then incubated with Human tumor dissociation kit (Miltenyi Biotec, #130-095-929) and placed at 37°C in agitation (500 rpm ...
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bioRxiv - Cell Biology 2023Quote: ... cut longitudinally into roughly 2-mm-long pieces and were isolated using the Liver Dissociation Kit (mouse) and GentleMACS Dissociator (Miltenyi Biotec, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... + 0.5x GlutaMax (Thermo 35050061) + 1x NEAA (Thermo 11140050) + 1x N-2 (Thermo 17502048) + 1x MACS NeuroBrew-21 (Miltenyi Biotec 130-093-566). On D3 ...
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bioRxiv - Cell Biology 2023Quote: ... tissues were cleansed in ice-cold RPMI1640 and dissociated using the Multi-tissue dissociation kit 2 (Catalog No.130-110-203, Miltenyi Biotec, USA) following the manufacturer instructions ...
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bioRxiv - Immunology 2024Quote: CD4+ and CD8+ T cells and CD14+ monocytes were isolated from healthy donor mononuclear cells (agreement ANG-2003-2) by positive magnetic sorting (Miltenyi Biotec, Germany). Macrophages were generated by culturing monocytes for 7 days with 50 ng/ml M-CSF or 50 ng/ml GM-CSF (ORF Genetics Kopavogur ...
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bioRxiv - Immunology 2024Quote: ... both CD45.2 DKO and WT polyclonal donor marrow was depleted of mature T cells by magnetic column-based negative selection (Miltenyi Biotec MACS) using an anti-mouse CD5 biotin-conjugated antibody (Clone 53-7.3 ...
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bioRxiv - Immunology 2021Quote: ... Cells were stimulated for 6 hours with 2 μg/ml of each of the 15 matrix peptide pools in the presence of 1 µg/ml anti-CD40 antibody (HB14) (purchased from Miltenyi Biotec, Bergisch Gladbach, Germany) and 1 μg/ml anti-CD28 antibody (CD28.2 ...
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bioRxiv - Immunology 2022Quote: ... anti-CD158a/h (KIR2DL1/DS1) VioBright 515 (clone REA1010, Miltenyi Biotec), anti-CD158b (KIR2DL2/DL3 ...
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bioRxiv - Immunology 2022Quote: ... anti-CD158a/h (KIR2DL1/DS1) VioBright 515 (clone REA1010, Miltenyi Biotec), anti-CD158b (KIR2DL2/DL3 ...
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bioRxiv - Developmental Biology 2020Quote: ... and 3 μM CHIR99021 (Miltenyi Biotec), together abbreviated as 2i ...
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bioRxiv - Neuroscience 2022Quote: ... and 3 μM CHIR99021 (Miltenyi Biotec) were added to the medium ...
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bioRxiv - Developmental Biology 2022Quote: ... and 3 μM CHIR99021 (Miltenyi Biotec). Cells were maintained at 37°C with 6% CO2.
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bioRxiv - Cell Biology 2020Quote: ... CHIR99021 (CH, 3 μM, Miltenyi Biotec) and leukemia inhibitory factor (LIF ...
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bioRxiv - Cell Biology 2020Quote: ... 3 μM CHIR 99021 (Miltenyi Biotec) and 150 μM LAAP (Merck) ...
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bioRxiv - Neuroscience 2024Quote: ... and 3 μM CHIR99021 (Miltenyi Biotec) were also added to the medium ...
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bioRxiv - Microbiology 2020Quote: ... One gram of each tissue was homogenized in 9 ml of PBS in gentleMACS M tubes using the appropriate setting on the gentleMACS dissociator (Miltenyi Biotec). Homogenates were filtered through 40-μm-pore-size filters and an aliquot was used to determine viable counts ...
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bioRxiv - Immunology 2023Quote: ... and GC B cells were enriched on day 9 by depleting all CD43+ and all IgD+ cells with anti-mouse CD43 microbeads (Catalog:130-097-148, Miltenyi Biotec) and biotinylated anti-mouse IgD/anti-biotin microbeads (Catalog:130-090-485 ...
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bioRxiv - Cell Biology 2022Quote: ... 2 mg of each cell lysate was incubated with 40 μL Anti-HA Microbeads (μMACS HA Isolation Kit, Miltenyi Biotec, 130-091-122) in a volume of 500 μL for 45 min at 4 °C with rotation ...
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bioRxiv - Developmental Biology 2020Quote: ... cell suspension with PBS supplemented with 0.5% BSA and 2 mM EDTA was applied to an MS column (Miltenyi Biotec, 130-042-201). After negative cells flow through the column ...
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bioRxiv - Microbiology 2024Quote: ... The samples were investigated at RT with 2-Photon imaging using two laser lines in parallel and PBS as the optical medium (LaVision Bio Tec TrimpScope 2; Miltenyi Biotec, Bielefeld, Germany). mCherry was excited with a Spectra-Physics Insight laser set to 1105 nm and GFP and dermal collagen were visualized by a second laser set to 910 nm at an intensity of 1 to 10% of its maximum power ...
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bioRxiv - Neuroscience 2024Quote: ... + 0.5% bovine serum albumin (BSA) + 2 mM EDTA + 0.05% sodium azide) and incubated with 25 μL CD11b (Microglia) microbeads (Miltenyi Biotec, #130-093-634) on ice for 30 min ...
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bioRxiv - Neuroscience 2021Quote: ... a 5 µl volume of phosphate-buffered PBS (PBS) containing 10 μg/kg/injection of recombinant mouse IL-1β (Miltenyi Biotec, Bergisch Gladbach, Germany) or of PBS alone (control ...
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bioRxiv - Molecular Biology 2024Quote: ... MDSL and TF-1 were additionally supplemented with 25ng/mL of recombinant human IL-3 (Miltenyi Biotec, 130-095-069), and MDS-L with 50nM β-mercaptoethanol (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... each blood sample was diluted in a 1 to 1 ratio with the HBSS buffer solution and incubated with 10% EpCAM MicroBeads (Miltenyi Biotec) with shaking at room temperature for an hour ...