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Citations for Miltenyi Biotec :
351 - 400 of 652 citations for Siglec 15 Human HEK293 mFc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... MP1 and SARS-CoV-2 Prot_S (SPIKE) (peptides pools of 15 amino acids length with 11 amino acid overlap, Miltenyi Biotec) in 5% human serum RPMI medium in the presence of 1µg/ml anti-CD40 (HB14 ...
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bioRxiv - Cell Biology 2020Quote: ... cells were washed three times and incubated in PBS containing DAPI (1 μg/mL) for 15 min before samples were processed using flow cytometry (MACSQuant, Miltenyi Biotec). At least 10000 events were analyzed per sample using FlowLogic software (Miltenyi Biotec).
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bioRxiv - Immunology 2019Quote: HIV-1-infected or mock-infected CD8-depleted PBMCs cells were used as targets and autologous cryo-preserved PBMCs rested overnight in R10 supplemented with 10ng/ml of IL-15 (Miltenyi Biotec) were used as a source of effector cells ...
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bioRxiv - Cancer Biology 2021Quote: ... were added to the nucleofected cells at the ratio of 15:1 in the presence of anti-CD3 antibody (50 ng/ml, 130-093-387, Miltenyi Biotec) and IL-2 (300 IU/ml ...
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bioRxiv - Immunology 2020Quote: ... Cells were then washed three times and cultured with 1 ng/mL rhIL-15 or 20 IU/mL rhIL-2 (Miltenyi Biotec). After 3 days ...
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bioRxiv - Immunology 2022Quote: ... we assessed the capacity of NK cells to perform ADCC after being stimulated with IFN-α or IL-15 (Miltenyi Biotec). In such cases ...
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bioRxiv - Cell Biology 2022Quote: ... incubated for 15 min at room temperature and applied onto the dead cell removal column (cat # 130-042-401, Miltenyi Biotec), which was attached to the MACS separator ...
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bioRxiv - Immunology 2020Quote: ... sequences [SLA (p1-p53)] or 0.6 nmol/mL PepTivatorR Candida albicans MP65 (peptides pools of 15 amino acids length with 11 amino acid overlap, Miltenyi Biotec) in 5% human serum RPMI medium in the presence of 1µg/ml anti-CD40 (HB14 ...
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bioRxiv - Genetics 2021Quote: ... Lysates were centrifuged at 4,000 x g and 4°C for 15 min and the supernatant was incubated with 50 µl magnetic beads coupled to monoclonal mouse anti-GFP antibody (Miltenyi Biotec) for 20 min on ice ...
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bioRxiv - Cancer Biology 2020Quote: ... 0.5% in PBS1X at 4°C for 15’ and washed with PBS1X before being resuspended in autoMACS Running Buffer (Miltenyi Biotec). Immunostained CD34+ cells were then subjected to single cell sorting by means of DEPArray™ Technology (Menarini Silicon Biosystems) ...
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bioRxiv - Immunology 2021Quote: ... Samples were then transferred to 15 mL conical tubes and non-memory B cells were depleted using the AutoMACS Magnetic Sorter (Miltenyi Biotec) “deplete” program ...
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bioRxiv - Cell Biology 2022Quote: ... JAM-1 and CD45 (Fig. 3, Supplementary Table 4) for 15 min prior to analysis on a MACSQuant10 Flow Analyzer (Miltenyi Biotec). To determine proportions of SCs and FAPs (Fig ...
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bioRxiv - Neuroscience 2022Quote: ... followed by a 15 min incubation with 10 µL microbeads mixture containing antibodies directed against CD140 (130-101-502, Miltenyi Biotec), NG2 (130-097-170 ...
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bioRxiv - Immunology 2022Quote: ... antibodies in T cell media (RPMI containing 10% FBS, 2mM L-Glutamine, 1% Penicilin-Streptomycin) with IL-15 and IL-7 (Miltenyi Biotec, 10ng/ml each ...
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bioRxiv - Immunology 2023Quote: ... and incubated at 37°C for total of 30 minutes (15 minutes, 2 times) with rotated using MACSmix Tube Rotator (Miltenyi Biotec). Between and after the two incubations ...
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bioRxiv - Biophysics 2023Quote: ... antibodies in T cell media (RPMI 10 % FBS, 2 mM L-Glutamine, 1 % Penicillin-Streptomycin) with IL-15 and IL-7 (Miltenyi Biotec, 10 ng/ml each ...
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bioRxiv - Genetics 2023Quote: ... 15 mins) and resuspended in 3mL DPBS supplemented with 30 µL Viobility 405/452 Fixable Dye (Miltenyi Biotec, 130-130-420). All the following steps were done in the dark ...
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bioRxiv - Molecular Biology 2024Quote: ... the cell pellets were subjected to a 15-minute staining procedure in the dark using Anti-CD11b Magnetic Microbeads (130–093-634, Miltenyi Biotec) on ice ...
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bioRxiv - Biochemistry 2019Quote: ... and subsequent CD14+ positive selection using the MACS Miltenyi Biotec Human CD14 microbead protocol (Miltenyi Biotec). CD14+ cells were differentiated in to macrophages using recombinant human granulocyte-macrophage colony-stimulating factor (200 ng/mL GM-CSF ...
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bioRxiv - Cell Biology 2021Quote: ... and 60 ng/mL of human receptor activator of NF-κB-ligand (RANK-L, Miltenyi Biotec). The medium was then changed every third day by RPMI containing 10% FCS ...
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bioRxiv - Cell Biology 2020Quote: ... or 20 ng/ml PDGF-AA (human PDGF-AA, Cat nb. 130-108-983, Miltenyi Biotec). Inhibition of PDGF-signaling was performed by addition of 1μM imatinib (Cat nb ...
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bioRxiv - Neuroscience 2022Quote: ... the cells were labeled with human CD11b-APCVio770 and CD45-phycoerythrin (PE) (Miltenyi Biotec, Gladbach, Germany), and flow cytometry was performed as described previously (38) ...
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bioRxiv - Microbiology 2022Quote: ... Bacteria were stained with PE Anti-human IgA antibodies (1:100; Miltenyi Biotec clone IS11-8E10) for 30 min on ice ...
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bioRxiv - Immunology 2020Quote: ... T cells were isolated from PBMC using the Human Pan T cell isolation kit (Miltenyi Biotec) and subsequently activated by TransAct (Miltenyi Biotec) ...
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bioRxiv - Immunology 2020Quote: ... The cells were isolated using a human negative selection-based NK isolation kit (RosetteSep, Miltenyi Biotec). The purified NK cells were then cultured in a stem cell serum-free growth medium (CellGenix GMP SCGM ...
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bioRxiv - Immunology 2022Quote: ... B cells were first isolated from donors’ PBMC by MACS using human CD19 MicroBeads (Miltenyi Biotec). CD19+ B cells were then stained using LIVE/DEAD aqua fixable dead cell stain kit (Molecular Probes ...
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bioRxiv - Immunology 2022Quote: Breast tissue was enzymatically and mechanically dissociated as per the Human Tumor Dissociation Kit (Miltenyi Biotec) protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... we also used CD3-PE to specifically identify lymphocytes (human antibody, clone BW264/56, Miltenyi Biotec). For all the samples ...
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bioRxiv - Immunology 2020Quote: ... CD14+ cells were isolated using the MACS human CD14+ isolation kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2020Quote: ... and the media was further supplemented with 100 U/ml human IL-2 IS (Miltenyi Biotec), 1 ng/ml IL-15 (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2020Quote: ... Primary human CD4+ T cells were isolated by untouched CD4+ T cell isolation kit (Miltenyi Biotec). Human CD4+ T cells were activated by Dynabeads Human T-Activator CD3/CD28 (ThermoFisher ...
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bioRxiv - Immunology 2020Quote: For cultured human lung macrophages staining: anti-CD169 (7-239) and anti-CD11c (REA618, Miltenyi Biotec) were used for membrane staining ...
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bioRxiv - Immunology 2020Quote: ... CD8+ T cells were purified from PBMC via human CD8 MicroBeads (Miltenyi Biotec, 130-045-201) and activated with T Cell TransAct (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... CD3 selection was performed after being transduced with γδTCR-Cl5 using human CD3 microbeads (Miltenyi Biotec). Moreover ...
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bioRxiv - Immunology 2020Quote: ... Immature human moDCs were generated from PBMCs using CD14 Microbeads (Miltenyi Biotec cat. 130-050-201) and cultured for 6 days with recombinant GM-CSF (R&D Systems cat ...
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bioRxiv - Microbiology 2023Quote: ... CD4+ T cells were isolated by positive selection (using magnetic CD4 human MicroBeads from Miltenyi Biotec). Cells were activated with CD3/CD28 agonists (T Cell transact ...
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bioRxiv - Microbiology 2022Quote: ... and human granulocyte-macrophage colony-stimulating factor (GM-CSF; 20ng/ml; MILTENYI BIOTEC; 130-093-866). At day 5 ...
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bioRxiv - Immunology 2024Quote: B cells were eluted from PBMCs using a MACS Human B Cell isolation kit (Miltenyi Biotec). B cells were stained with rGP38 (IbAr10200 ...
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bioRxiv - Cell Biology 2019Quote: ... The suspension was incubated for 15 min with dead cell removal paramagnetic beads (130-090-101, Miltenyi Biotec GmbH, Bergisch Gladbach, Germany) and then passed through LS column (130-042-401 ...
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bioRxiv - Neuroscience 2023Quote: ... cells were resuspended in PBS with 0.5% of Bovine Serum Albumin (BSA, pH 7.2) and incubated for 15 min at 4 °C with myelin removal beads II (Miltenyi Biotec, Cologne, Germany) for myelin and debris removal ...
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bioRxiv - Physiology 2023Quote: ... The suspension was incubated for 15 min with dead cell removal paramagnetic beads (130-090-101, Miltenyi Biotec GmbH, Bergisch Gladbach, Germany) and then passed through LS column (130-042-401 ...
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bioRxiv - Molecular Biology 2024Quote: ... pooled honey bees (n=30) were dissociated with 15 mL of molecular grade water using gentleMACS™ M Tubes (Miltenyi Biotec, USA) and a gentleMACS™ Octo Dissociator (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: Naive B cells were purified from PBMCs using the MACS Human B Cell isolation kit (Miltenyi Biotec) and incubated with 25nM of each SARS-CoV-2 probe (RBD-mFc-BV650 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Primary human T cells were purified from PBMCs by Pan T Cell Isolation Kit (Miltenyi Biotec, Germany). Purified T cells were stimulated with anti-CD3/CD28 Dynabeads (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... CD14+ monocytes were isolated from PBMCs using human anti-CD14 magnetic beads with LS Columns (Miltenyi Biotec). CD14+ monocytes were differentiated into MDM in RPMI 1640 medium supplemented with 10% FBS ...
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bioRxiv - Immunology 2020Quote: ... NK cells were isolated from PBMCs by negative depletion with human NK cell Isolation Kit (Miltenyi Biotec). Cells were cultured in RPMI 1640 medium supplemented with GlutaMAX (Gibco) ...
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bioRxiv - Cancer Biology 2022Quote: ... freshly isolated tumor samples were dissociated using the human tumor dissociation kit (Miltenyi Biotec; 130-095-929): surgical resection tissue was first minced into small pieces using a scalpel ...
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bioRxiv - Cancer Biology 2022Quote: ... 25 μL Enzyme A (all provided in the human tumor dissociation kit (Miltenyi Biotec; 130-095-929), and 4.7 mL DMEM (Gibco ...
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bioRxiv - Immunology 2022Quote: ... 100,000 cells per well were activated with anti-human TransAct (1:100, Miltenyi Biotec 130-111-160) in T cell medium (10 % FCS ...
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bioRxiv - Cancer Biology 2022Quote: ... Anti-mouse and anti-human FcyR blocking antibodies were used to prevent non-specific binding (Miltenyi Biotec). 7-aminoactinomycin D (BD ...