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Citations for Miltenyi Biotec :
351 - 400 of 669 citations for Recombinant Mouse Regenerating Islet derived 3 Alpha His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... before dissociating using an Adipose Tissue Dissociation Kit for Mouse and Rat (Miltenyi Biotec), using a gentleMACS™ Octo Dissociator with Heaters (Miltenyi Biotec) ...
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bioRxiv - Immunology 2023Quote: ... and biotinylated anti-mouse IgD/anti-biotin microbeads (Catalog:130-090-485, Miltenyi Biotec). Cells were pooled from 3 donor mice of each genotype in each experiment ...
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bioRxiv - Neuroscience 2023Quote: Mouse brains were dissociated with Adult Brain Dissociation kit (Miltenyi Biotec, 130-107- 677) and incubated with the coupled antibody NeuN Alexa 647 (Abcam ...
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bioRxiv - Pathology 2024Quote: ... followed by incubation with anti-mouse FcR blocking reagent (130-092-575, Miltenyi Biotec) in PBS 2% FBS for 10 min at 4°C ...
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BTK and MMP9 regulate NLRP3 inflammasome-dependent cytokine and NET responses in primary neutrophilsbioRxiv - Immunology 2024Quote: ... and the neutrophils were isolated using magnetic separation (mouse neutrophil isolation kit, Miltenyi Biotec) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Neural cells were acutely isolated from adult mouse brain using MACS technology (Miltenyi Biotec). Brain tissue was dissociated into a single-cell suspension by enzymatic digestion using the Adult Brain Dissociation Kit P (# 130-107-677 ...
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bioRxiv - Cell Biology 2024Quote: ... and mouse anti-GFP HRP-conjugated at 1:5,000 (130-091-833, Miltenyi Biotec). Chemiluminescence detection was conducted using SuperSignal West Femto Maximum Sensitivity Substrate (34096 ...
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bioRxiv - Immunology 2024Quote: Resting B lymphocytes were isolated from mouse spleens using anti-CD43 MicroBeads (Miltenyi Biotec), and grown in RPMI 1640 medium (Life Technologies ...
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bioRxiv - Cancer Biology 2024Quote: Primary resting mouse splenic B-cells were isolated using anti-CD43 microbeads (Miltenyi Biotec) and activated with 25 ug/mL LPS (Sigma) ...
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bioRxiv - Immunology 2022Quote: ... (3) Non-SARS-CoV-2 antigens: Peptide pools of the pp65 protein of human cytomegalovirus (CMV) (Miltenyi Biotec, Gladbach, GER), or HIV-1 gag peptide pools (NIH AIDS Reagent Repository ...
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bioRxiv - Neuroscience 2023Quote: ... frozen hippocampus of 3 mice in each sample group were dissociated using the Adult Brain Dissociation kit (Miltenyi Biotec, Germany), and after dead cell removal ...
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bioRxiv - Cancer Biology 2023Quote: ... and 3) removal of debris and dead cells using the Dead Cell Removal Kit (Miltenyi Biotec, Cat: 130-090-101). The brain of an age-matched uninoculated mouse was harvested and processed in parallel to serve as the negative control for downstream FACS sorting ...
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bioRxiv - Cancer Biology 2024Quote: ... tumors were harvested from mice on Day 3 and separately dissociated on a GentleMacs Octo Dissociator with Heaters (Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were washed in 1%FBS in Dulbecco’s PBS 3 times before straining through 75 µm and 30 µm strainers (Greiner Bio-One and Miltenyi Biotec) in Dulbecco’s PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were mixed with a 20μl cocktail from a Mouse Cell Depletion Kit (Miltenyi Biotec). Separation of mouse cells from human tumor cells was achieved using an LS column on a MACS Separator (Miltenyi Biotec) ...
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bioRxiv - Molecular Biology 2021Quote: ... lineage negative BM cells were first depleted using mouse lineage cell depletion kit (Miltenyi Biotec) then applied to five-laser FACSAria II sorter (BD Biosciences).
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bioRxiv - Developmental Biology 2020Quote: ... Samples resuspended in 2% FBS/PBS solution were blocked with mouse Fc Block (Miltenyi Biotec) for 5 minutes on ice ...
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bioRxiv - Molecular Biology 2022Quote: ... resting naive mouse B cells were isolated from splenocytes with anti-CD43 MicroBeads (Miltenyi Biotec) by negative selection and activated for 72 hr in the presence of LPS (50 μg/ml final concentration ...
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bioRxiv - Immunology 2022Quote: ... IFN-γ secreting cells were labeled using the mouse IFN-γ secretion assay (Miltenyi Biotec) and then stained for viability and CD8 ...
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bioRxiv - Genetics 2022Quote: Mouse tissue was dissociated using gentleMACS Dissociator (Miltenyi Biotec Inc., cat. no. 130-093-235) and gentleMACS M Tubes (Miltenyi Biotec Inc. ...
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bioRxiv - Systems Biology 2019Quote: ... cell suspensions were stained with anti-mouse CD45-PE-Vio770 (Miltenyi Biotec, 130-110-661), CD326-BV421 (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... Lungs were processed into single cell suspension using the mouse lung dissociation kit (Miltenyi Biotec). The following fluorochrome-conjugated antibodies were used for labelling the cells ...
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bioRxiv - Microbiology 2021Quote: ... a 1:20 dilution of APC-conjugated mouse anti-CD163 (clone REA812, MACS Miltenyi Biotec), 0.14 µg/mL PE-conjugated mouse anti-HLA-DR (clone LN3 ...
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bioRxiv - Immunology 2021Quote: Lung epithelial cells separated by magnetic bead: the anti-mouse CD45 magnetic bead (Miltenyi Biotec) was used to enrich the epithelial cells in single-cell suspension ...
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bioRxiv - Immunology 2022Quote: ... samples were first positively selected for CD11c+ cells using Mouse UltraPure CD11c Microbeads (Miltenyi Biotec) prior to surface marker staining ...
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bioRxiv - Neuroscience 2022Quote: ... Microglia and myelin debris were subsequently removed through a mouse anti-CD45 (Invitrogen)-coated dish and myelin removal beads (Myelin Removal Beads II, human, mouse, rat, Miltenyi Biotec), respectively ...
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bioRxiv - Immunology 2022Quote: ... mouse splenocytes were subjected to ACK lysis followed by anti-CD4 positive selection (Miltenyi Biotec). Naive CD4+ T cells (CD4+CD25-CD44-CD62L+ ...
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bioRxiv - Immunology 2023Quote: B cells were isolated with anti-mouse CD19 microbeads (Catalog:130-097-144, Miltenyi Biotec) from Cd79acre/+Zbtb18fl/fl and Cd79acre/+ mice ...
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bioRxiv - Immunology 2024Quote: ... Mouse whole brains were processed using a brain dissociation kit (Miltenyi Biotec, 130-107-677) with the gentleMACS Octo Dissociator (Miltenyi Biotec,130-096-427) ...
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bioRxiv - Immunology 2019Quote: CD4+ and CD14+ cells were enriched from the PBMCs of 3 healthy donors via magnetic separation using the corresponding MicroBeads (Miltenyi Biotec). The CD14+ fraction was cryopreserved in liquid nitrogen ...
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bioRxiv - Cell Biology 2020Quote: Pre-conditioned media from FAP cell culture stimulated 3 days with PDGF-AA (20 ng/ml, human PDGF-AA, Cat nb. 130-108-983, Miltenyi Biotec) or control was collected and stored at −20°C ...
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bioRxiv - Immunology 2021Quote: ... T cells were activated with T cell activation/expansion CD2/3/28 beads at a ratio of 1:2 bead to cells (Miltenyi Biotec). Cultures were maintained in HPLM media with 5% dialyzed FBS (Sigma Aldrich ...
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bioRxiv - Immunology 2022Quote: ... supplemented with cytokines (IL-6 10ng/ml, IL-3 20ng/ml, M-CSF 50ng/ml and SCF 20ng/ml; all Miltenyi Biotec), with 2.5% Pen/Strep and 20μM L-Glutamine (Sigma-Aldrich) ...
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bioRxiv - Genetics 2019Quote: ... The flow-through was pelleted at 1250 rpm in a swinging bucket centrifuge set to 6°C then resuspended in 3 ml cold AutoMACs Running Buffer (AMB, Miltenyi Biotec). After gentle resuspension ...
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bioRxiv - Developmental Biology 2020Quote: ... Cell clumps and debris were removed by two consecutive rounds of centrifugation 3 minutes at 200xg and filtration through 30 µm cell strainers (Miltenyi Biotec). Additional mechanical dissociation and removal of cell aggregates was achieved by gentle pipetting up and down 5 times using a P200 pipette and Gel Saver tips (QSP) ...
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bioRxiv - Immunology 2020Quote: ... after which 3 mL of FACS buffer was added and the mixture was passed over a magnetized LS column (Miltenyi Biotec). The column was washed once with 5 mL ice-cold FACS buffer and then removed from the magnetic field and 5 mL ice-cold FACS buffer was pushed through the unmagnetized column twice using a plunger to elute the bound cell fraction.
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bioRxiv - Immunology 2021Quote: ... 21 and 63 DPI samples (n=3) were depleted of alveolar macrophages using a MACS cell separation LS column (Miltenyi Biotec) after staining for CD14 and CD172a (see Table S2) ...
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bioRxiv - Genomics 2021Quote: ... cells were blocked with 3%BSA diluted in PBS for 1h at RT and then incubated with anti-CD-19-PE antibody (1:50) in 3% BSA-PBS (Miltenyi Biotec) for 1h at RT ...
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bioRxiv - Developmental Biology 2022Quote: The soft palate from E13.5 embryos was dissected and digested using Multi Tissue Dissociate Kit 3 (Miltenyi Biotec, 130-110-204) at 37°C (600 rpm for 15 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... Remaining clumps of cells and debris were removed via two rounds of centrifugation for 3 min at 200 g followed by filtration through 30 μm cell strainers (Miltenyi Biotec). To ensure all remaining cells aggregates were dissociated ...
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bioRxiv - Biophysics 2022Quote: ... Naive T cells were purified via magnetic negative selection from spleens of 6–8 week old mice using either CD4+ (for 3.L2) or CD8+ (for P14 and OT1) T-cell isolation kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... JAM-1 and CD45 (Fig. 3, Supplementary Table 4) for 15 min prior to analysis on a MACSQuant10 Flow Analyzer (Miltenyi Biotec). To determine proportions of SCs and FAPs (Fig ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 to 7 Nematostella polyps or 3 Aiptasia polyps constituted one biological sample and were transferred to tissue homogenization tubes (“C-tubes”, 130-096-334, Miltenyi Biotec) after they were relaxed ...
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bioRxiv - Bioengineering 2023Quote: ... and 2/3 for CD4+ subsets (both positive selection for CD4+CD25+cells and negative selection for CD4+CD25- cells Miltenyi Biotec). A small portion of each cell fraction was analyzed by flow cytometry to confirm enrichment of the desired cell subtype ...
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bioRxiv - Cancer Biology 2022Quote: ... standard CAR-T cells for the clinical setting were produced except for several modifications(20): CD8+ T cells were stimulated with 1 ug/ml OKT-3/CD28 antibodies (Miltenyi Biotec) for 72 h ...
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bioRxiv - Molecular Biology 2024Quote: ... program and cultured in TexMACS supplemented with 3% human serum (HS) (Life Science Production) and interleukin-2 (IL-2) (Miltenyi Biotec) for 24 hours ...
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bioRxiv - Immunology 2019Quote: ... B cells were isolated from total splenocytes using naïve mouse B-cell isolation kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Cells (106) were stained with the ‘Mouse IFN-γ secretion assay detection kit’ (Miltenyi Biotec, Germany) according to the manufacturer’s instructions and IFN-γ secretion was analyzed by flow cytometry.
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bioRxiv - Cancer Biology 2020Quote: ... tumors were harvested and dissociated using a mouse tumor dissociation kit (Miltenyi Biotec, 130-096-730). After dissociation erythrocytes were lysed with Red Cell Lysis Buffer (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2021Quote: ... Mouse splenic T cells were isolated with a Pan T Cell Isolation Kit II (Miltenyi Biotec). Jurkat T cells and Raji B cells were cultured in complete RPMI-1640 (Hyclone ...