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Citations for Miltenyi Biotec :
351 - 400 of 1969 citations for Rat C Reactive Protein ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Subsequent washing with MACS buffer (PBS containing 2 mM EDTA and 0.5% BSA) was followed by a 15-minute incubation at 10°C with anti-IgG magnetic beads (Miltenyi Biotec). The cell suspension was introduced to a LS separation column following the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: ... In short small pieces of full-thickness specimens were placed in a gentleMACS C-tube (130-093-237, Miltenyi Biotec) in digestion solution and dissociated in the gentleMACS Octo Dissociator (130-095-937 ...
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bioRxiv - Immunology 2023Quote: Processing of peripheral and cryopreservation was completed as previously described.8 Fresh tumor tissue was diced with a sterile scalpel and dissociated in 0.1% collagenase in RPMI 1640 for 60 minutes at 37⁰C using the gentleMACS OctoDissociator (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... PBMC were incubated at 37°C for 15 minutes with 0.5 μg/mL anti-human CD40 mAb blocking antibody (Miltenyi Biotec). Chemokine receptor antibodies were also added on day 1 (see ref23 for antibodies used in the AIM assay) ...
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bioRxiv - Biochemistry 2024Quote: ... cells were incubated for 20 min at 4°C with an anti-mouse antibody conjugated to magnetic microbeads (Miltenyi Biotec). Finally ...
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bioRxiv - Systems Biology 2024Quote: ... tumor was cut into 1-2 mm pieces and transferred to a gentleMACS C Tube (Miltenyi Biotec #130-093-237) containing 4.7 mL of sterile media (Knockout DMEM/F12) ...
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bioRxiv - Neuroscience 2024Quote: ... C-tubes tightly closed and attached upside down onto the sleeve of the gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec.). The appropriate program 37C_ABDK_02 was run for 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... M and N protein pools [1 µg/mL] (PepTivator® SARS-CoV-2 regents; Miltenyi Biotec) in 96-well U-bottom plates at 0,5-1×106 PBMCs per well ...
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bioRxiv - Plant Biology 2021Quote: ... H-tagged proteins were detected using the anti-HA HRP-coupled antibody (mouse monoclonal, Miltenyi Biotec) diluted 1:5000 (v/v) ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins were subsequently detected with horseradish peroxidase (HRP)-conjugated anti-GFP antibody (1:5,000) (Miltenyi Biotec) for 1 hr at room temperature with gentle shaking ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were subsequently detected with horseradish peroxidase (HRP)-conjugated anti-GFP antibody (1:5,000) (Miltenyi Biotec) or HRP-conjugated anti-c-myc antibody (1:5,000 ...
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bioRxiv - Immunology 2022Quote: ... the wound tissues were firstly digested by the Epidermis Dissociation Kit (Epidermis Dissociation Kit, mouse; Miltenyi Biotec) for enzymatic epidermal-dermal separation ...
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bioRxiv - Immunology 2023Quote: Human CD4+ T cells were isolated by employing a CD4+ T cells isolation kit (Miltenyi Biotec kit) following the provider’s instructions ...
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bioRxiv - Immunology 2023Quote: ... the wound samples were enzymatically digested by the Epidermis Dissociation Kit (Epidermis Dissociation Kit, mouse; Miltenyi Biotec) to achieve the epidermal-dermal separation ...
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bioRxiv - Cancer Biology 2021Quote: Freshly resected tumor or adjacent normal lung tissue was thoroughly chopped with surgical scissors and transferred to GentleMACS C Tubes (Miltenyi Biotec) containing 20 uL/mL Liberase TL (5 mg/ml ...
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bioRxiv - Synthetic Biology 2019Quote: ... Cultures were grown overnight at 37 °C and shaking and then diluted in 1:50 in DPBS and analyzed on a MACSQuant VYB flow cytometer (Miltenyi Biotec) using a previously described strategy to gate for single cells3 ...
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bioRxiv - Microbiology 2021Quote: ... Samples were stored at 4°C in dark until the next morning for data acquisition using a MacQuant10 (Miltenyi Biotec, USA). Data was analyzed using FlowJo 10 (BD Biosciences ...
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bioRxiv - Cancer Biology 2022Quote: Cryopreserved grafts were thawed and stained using standard procedures (10 min, 4°C) with the surface antibody CD34-PE-Vio770 (clone AC136, lot# 5180718070, dilution 1:50, Miltenyi Biotec) and DAPI (Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2022Quote: ... and then incubated for 2h at 4°C with 50 µl of anti-GFP antibody conjugated with paramagnetic beads (Miltenyi Biotec). After incubation ...
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bioRxiv - Neuroscience 2022Quote: ... the contents of the gentleMACS™ C Tube were strained through a 70 μm cell strainer (Miltenyi Biotec, # 130-110-916) placed on top of a 15 mL conical centrifuge tube ...
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bioRxiv - Genetics 2022Quote: ... kidneys were quickly dissected after mouse euthanization and transferred to gentleMACS C Tubes (Miltenyi Biotec Inc., cat. no. # 130-093-237) in 2ml of digestion buffer (13.5 ml of Dispase (STEMCELL ...
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bioRxiv - Genetics 2019Quote: ... The flow-through was pelleted at 1250 rpm in a swinging bucket centrifuge set to 6°C then resuspended in 3 ml cold AutoMACs Running Buffer (AMB, Miltenyi Biotec). After gentle resuspension ...
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bioRxiv - Physiology 2021Quote: ... The muscle was removed from the -80 °C freezer and transferred to -20 °C where it was minced into pieces of 1-2 mm before being transferred to gentleMACS M-tubes (Miltenyi Biotec) tubes kept on ice ...
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bioRxiv - Cell Biology 2020Quote: ... Mechanical and enzymatic muscle digestion was then performed at 37°C on the gentleMACS with heaters (cat nb. 130-096-427, Miltenyi Biotec) for 60 min using a skeletal muscle digestion program (37C_mr_SMDK1).
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bioRxiv - Cancer Biology 2020Quote: ... 0.5% in PBS1X at 4°C for 15’ and washed with PBS1X before being resuspended in autoMACS Running Buffer (Miltenyi Biotec). Immunostained CD34+ cells were then subjected to single cell sorting by means of DEPArray™ Technology (Menarini Silicon Biosystems) ...
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bioRxiv - Cancer Biology 2021Quote: ... we dissociated tumor cells from a fresh primary HB tumor using an enzyme mix in a gentleMACS C Tube (Miltenyi Biotec). Cells were spun ...
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bioRxiv - Immunology 2021Quote: ... and transplanted with syngeneic BALB/c bone marrow cells (5 × 106) after T-cell depletion using CD4 and CD8 MicroBeads (Miltenyi Biotec). For retransfer experiments ...
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bioRxiv - Immunology 2020Quote: ... cells were incubated at 37°C with 5% CO2 for 7 days and activated with M-CSF (1000 U/mL) (Miltenyi Biotec) every second day.
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bioRxiv - Immunology 2021Quote: ... spleens were harvested and single cell suspensions were incubated with APC-conjugated I-Ab Mtb Ag85b280-294 tetramer (provided by National Institutes of Health tetramer core facility) for 1 hour at 4°C and tetramer-positive cells were enriched using anti-APC Micro-Beads (Miltenyi Biotec). CD19−CD11c−CD11b−F4/80−CD8a− WT (CD45.1+CD45.2+ ...
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bioRxiv - Genomics 2022Quote: ... Cells were then stained with the following antibodies for 30 minutes at 4°C: CD3 VioGreen (clone BW264/56, Miltenyi Biotec), CD14 V500 (clone M5E2 ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were incubated for 72 hours at 37°C with either anti-CD3/anti-CD28 beads (1:2 bead-to-cell ratio, Miltenyi Biotec) or 200ng/mL N-803 in the presence of 50µM raltegravir and 5µM saquinavir ...
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bioRxiv - Genetics 2022Quote: ... Human tonsil tissue was processed by mincing with scissors followed by transfer of up to 4g of tissue to a gentleMACS C tube (Miltenyi Biotec) containing 8 mL of phosphate-buffered saline (PBS ...
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bioRxiv - Microbiology 2022Quote: ... After another incubation of 30 minutes at 37°C with 5% CO2 the samples are analysed by flow cytometry (MACS Quant, Miltenyi Biotec) and FlowJo™ v10.8 Software (BD Life Sciences) ...
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bioRxiv - Plant Biology 2022Quote: ... The lysates were clarified by centrifugation at 100,000 g for 20 min at 4°C and resulting the supernatant was incubated with 50 μL of anti-GFP magnetic beads (Miltenyi Biotec) for 30 min at 4°C with rotation (10 rpm) ...
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bioRxiv - Immunology 2022Quote: ... Suspensions were centrifuged at 400 × g and 4°C for 5 min and cells were suspended in autoMACS Running Buffer (RB, Miltenyi Biotec) followed by counting ...
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bioRxiv - Immunology 2022Quote: All cell culture was performed in a humidified incubator with 5% CO2 at 37°C using cRPMI (see above for ingredients) supplemented with 150ng/mL αCD40 (clone FGK-45; Miltenyi Biotec) and 25ng/mL recombinant murine IL-4 (Peprotech ...
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bioRxiv - Cancer Biology 2023Quote: ... Tumours were collected into cold PBS then manually cut into small pieces and digested in GentleMACS C dissociation tubes using the GentleMACS tissue dissociator (Miltenyi Biotec) with the manufacture’s enzymes (liver dissociation kit ...
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bioRxiv - Physiology 2023Quote: ... CD11b/c positive cells (monocyte/macrophage) were sorted using magnetic-activated cell sorting (MACS) separation columns (Miltenyi Biotec, 130-105-634) and immediately lysed with TRIzol (Fischer Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 to 7 Nematostella polyps or 3 Aiptasia polyps constituted one biological sample and were transferred to tissue homogenization tubes (“C-tubes”, 130-096-334, Miltenyi Biotec) after they were relaxed ...
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bioRxiv - Immunology 2023Quote: ... Cells (5×106) were infected at 5000 TCID50 / 1×106 cells at 37 °C for 2 hours on a cell-rotator (Miltenyi Biotec). After infection ...
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bioRxiv - Immunology 2023Quote: ... Fatty tissue was removed from the tonsil as much as possible with scalpels and placed into a gentleMACS C-tube (130-096-334, Miltenyi Biotec) with 5ml RPMI containing 0.3mg/ml Liberase (000000005401020001 Sigma ...
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bioRxiv - Neuroscience 2023Quote: The hippocampi from both hemispheres of Cx3cr1-NuTRAP mice were cut into four pieces and added to the pre-cooled gentleMACS C-tube (#130-096-334, Miltenyi Biotec) pre-filled with 2 mL ice-cold Nuclei Extraction Buffer (#130-128-024 ...
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bioRxiv - Immunology 2023Quote: ... Cells from BRAFi (n = 5) or BRAFi/C-Me (n = 6) tumors were pooled and CD45+ cells were isolated using CD45 MicroBeads (mouse, Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Lungs were then incubated at 37 C and dissociated using a GentleMACS Octo Dissociator with Heaters (Miltenyi Biotec #130-095-937). Cells were then filtered through a 70-micron mesh filter ...
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bioRxiv - Immunology 2023Quote: ... perfused murine lungs were placed in a gentle MACS C tube and mechanically homogenized in 5 ml of PBS using a gentle MACS Octo Dissociator (Miltenyi Biotec). Lung cell suspensions were lysed of RBCs ...
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bioRxiv - Immunology 2023Quote: ... Lungs were then incubated at 37 C and dissociated using a GentleMACS Octo Dissociator with Heaters (Miltenyi Biotec #130-095-937). Cells were then filtered through a 70-micron mesh filter ...
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bioRxiv - Immunology 2023Quote: ... splenocytes were resuspended in CTL test media (Immunospot) and incubated at 37 °C for 2 hours with either no stimulation or S1 peptide simulation (Miltenyi Biotec) at a final concentration of 2 µg/mL before adding GolgiPlug to all samples for 4 additional hours ...
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bioRxiv - Neuroscience 2023Quote: ... Each sample had 30□μl of Enzyme Mix 2 added before being mechanically dissociated for 30□min at 37°C on the gentleMACS Octo Dissociator with Heaters (#130-096-427, Miltenyi Biotec) using the 37C_ABDK_02 program ...
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bioRxiv - Immunology 2023Quote: ... and incubated at 37°C for total of 30 minutes (15 minutes, 2 times) with rotated using MACSmix Tube Rotator (Miltenyi Biotec). Between and after the two incubations ...
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bioRxiv - Cancer Biology 2023Quote: ... and an enzymatic cocktail (supplementary table 7) and digested at 37°C for 42 minutes (37C_m_TDK_2 program) on a gentleMACS Octo Dissociator (Miltenyi Biotec, 130-096-427). Cell suspensions were then filtered through a 40 μm cell strainer (Corning ...