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Citations for Miltenyi Biotec :
351 - 400 of 1068 citations for Human Chemokine C X C Motif Receptor 5 CXCR5 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Human (Miltenyi Biotec) for 60 min at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... human (Miltenyi Biotec) for 10 minutes at 4 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... Human (Miltenyi Biotec) according to the manufacturer’s recommendations ...
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bioRxiv - Bioengineering 2021Quote: ... and 5% Human AB serum (Gemini) supplemented with 50 units/mL IL-2 every 2 days for all experiments (Miltenyi Biotec).
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bioRxiv - Cancer Biology 2022Quote: ... we separate the epidermal tissue from the dermis and then dissociated to single-cell suspensions by combining mechanical dissociation with enzymatic degradation of the extracellular adhesion proteins using Epidermis Dissociation Kit for Human (130-103-464, MACS Miltenyi Biotec). The dissociated cell suspension was strained with a 40μm filter (BD Falcon) ...
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bioRxiv - Neuroscience 2024Quote: ... with Fc receptors blocking prior to Anti-ACSA-2 MicroBeads (Cat# 130-097-678, Miltenyi Biotec, Germany). Incubation was carried out for 15 minutes in the refrigerator at 4℃ ...
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bioRxiv - Microbiology 2021Quote: ... Fatty tissue was removed from the lymphoid tissues before dissecting the tissues into smaller pieces to allow dissociation using gentleMACS™ dissociation (C) tubes (Miltenyi Biotec) in Advanced RPMI medium supplemented with 10% fetal calf serum ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then stained with a CD34-PE-Vio770 antibody (20 minutes at 4°C; Miltenyi Biotec, clone AC136, #130-113-180) and DAPI (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... Tonsil and lung tissue samples were dissected to approximately 1 g pieces and placed into gentleMACS C Tubes (Miltenyi Biotec, Auburn, CA). The weight of each sample was recorded and PBS was added to a 10% weight/volume ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were spun at 400 g for 3 minutes at 4°C and washed in MACS Buffer (Miltenyi Biotec, 130-091-222) supplemented with 4% FBS (FBS ...
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bioRxiv - Neuroscience 2023Quote: ... cells were resuspended in PBS with 0.5% of Bovine Serum Albumin (BSA, pH 7.2) and incubated for 15 min at 4 °C with myelin removal beads II (Miltenyi Biotec, Cologne, Germany) for myelin and debris removal ...
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bioRxiv - Immunology 2022Quote: ... Yeast cells were washed 5 times with PBSF (PBS + 0.1% bovine serum albumin (BSA)) and 108 cells were incubated with 400 μL PBSF and 100 μL μMACS™ anti-c-Myc MicroBeads (Miltenyi Biotec) for 45 min on a rotator at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... Tumors were dissected from the limb and dissociated by shaking for 45 min at 37°C in an enzyme mix from the tumor dissociation kit (Miltenyi Biotec, 130096730). Cell suspension was then strained through a 40 µm filter ...
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bioRxiv - Bioengineering 2023Quote: ... Human TNF-α and human IFN-γ were purchased from Miltenyi Biotec. Human TNF-α DuoSet ELISA ...
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bioRxiv - Immunology 2021Quote: ... The large airways were removed and approximately ~500mg of airway free tissue was collected in a gentleMACS C-tube (Miltenyi Biotec, Auburn, CA) and 5mL digest buffer was added and placed on a gentleMACS octo dissociator for programmed runs ...
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bioRxiv - Microbiology 2019Quote: ... cells were either sub-cultured (Uns-C) or supplemented with kynurenic acid buffer and sorted according the manufacturer’s instructions using the Microbead Kit (Miltenyi Biotec #130-095-826). In brief ...
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bioRxiv - Immunology 2020Quote: ... 1% penicillin/streptomycin/L-glutamine)] prior to homogenisation in gentleMACS™ C-tubes using the gentleMACS™ dissociator (Miltenyi Biotec, Auburn, California, USA). Samples were incubated at 37°C with shaking to maximise digestion ...
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bioRxiv - Cell Biology 2020Quote: ... Single-cell suspensions were obtained by placing small sections of spleen (<5mm3) with cold phosphate buffer into gentleMACS™ C tubes and utilizing a benchtop gentleMACS™ dissociator (Miltenyi Biotec). MNCs were enriched through gradient separation (density gradient ...
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bioRxiv - Neuroscience 2022Quote: ... The remaining pellet was resuspended in 1.25 mL of Red Blood Cell Remover Solution and incubated for 10 minutes at 4 °C before the addition of 12 mL of 0.5 % bovine serum albumin (BSA, Miltenyi Biotec, # 130-091-376) diluted in DPBS ...
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bioRxiv - Systems Biology 2021Quote: ... Single-cell suspensions were obtained by enzymatically digesting the excised tumors using the Tumor Dissociation Kit and gentleMACS C system (Miltenyi Biotec, Auburn, CA). In addition to following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: Samples were processed immediately after surgery and temporarily stored for transport at 4°C in tissue storage solution (Miltenyi Biotec, Bergisch Gladbach, Germany) as previously described ...
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bioRxiv - Immunology 2023Quote: ... Cells harboring the receptor were enriched by using biotinylated anti-EGFR antibody and anti-biotin microbeads (Miltenyi Biotec).
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bioRxiv - Molecular Biology 2020Quote: ... human (Miltenyi Biotec Inc). See table S4 for antibodies used in flow cytometry.
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bioRxiv - Cell Biology 2022Quote: ... human (Miltenyi Biotec, USA) following the manufacturer’s recommendations ...
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bioRxiv - Physiology 2022Quote: ... human (Miltenyi Biotec, USA). Purified iPSC-cardiomyocytes were then plated on either Matrigel-coated glass coverslips (immature cells ...
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bioRxiv - Physiology 2022Quote: ... human (Miltenyi Biotec, USA) following the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2023Quote: ... human (Miltenyi Biotec, Germany) following protocols provided by the manufacturer ...
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bioRxiv - Molecular Biology 2020Quote: ... 3% human serum (Seralab) +20 ng/ml human recombinant IL-2 (Miltenyi Biotec) and activated with TransAct reagent (Miltenyi Biotec) ...
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bioRxiv - Microbiology 2021Quote: ... Lungs were removed from animals and the right lobes of the lung were homogenized in 1mL of PBS in Miltentyi C tubes (Miltenyi Biotec 130-096-334) using the m_lung_02 program on a Miltenyi gentleMACS tissue dissociator ...
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bioRxiv - Developmental Biology 2021Quote: ... Rhesus fetal lung tissue (~500 mg/tube) was cut into small pieces and transferred into a gentleMACS C-tube (Miltenyi Biotec, 130-093-237) containing 5 mL of digestion buffer (1 mL of Dispase [Corning ...
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bioRxiv - Microbiology 2020Quote: ... to analyze pDCs for 20 min at 4°C to analyze B cells or with two antibodies directed against CD303(BDCA2)-APC (Miltenyi Biotec, #130-097-931) and CD304(BDCA4)-PE (Miltenyi Biotec # 130-112-045) ...
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bioRxiv - Developmental Biology 2022Quote: ... The cells are centrifugated at 600 g for 7 min at 4 °C and resuspended with 180 μl running buffer (Miltenyi Biotec, 130-091-221) for MACS selection.
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bioRxiv - Immunology 2019Quote: ... centrifuged at 500g for 10 min at 4 °C and resuspended in 0XTD supplemented with 100 ng/ml each of Stem Cell Factor (SCF, Miltenyi Biotec, 130-096-695), Flt3 Ligand (Flt3L ...
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bioRxiv - Cancer Biology 2022Quote: ... samples were cut into approximately 2 – 4mm chunks with forceps and a scalpel and transferred to a gentleMACS C tube (Miltenyi Biotec, 130-093-237) containing 4.7 ml RPMI 1640 base media ...
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bioRxiv - Plant Biology 2022Quote: ... Extracted protein samples were centrifuged at 20 000g for 2 min at 4°C and the supernatant was collected for immunoprecipitation using the µMACS GFP Isolation Kit (Miltenyi Biotec, MediRay, New Zealand). Total and immunoprecipitated proteins were resolved on a 4–12% SDS-PAGE gel ...
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bioRxiv - Cancer Biology 2023Quote: ... For CD8+ T cells magnetic isolation we incubated the cell suspension 15 min incubation at 4°C with CD8 microbeads (Miltenyi Biotec, #130-045-201), then cells were passed through LS magnetic columns (Miltenyi Biotec ...
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bioRxiv - Developmental Biology 2024Quote: Dissected hearts were cut into small pieces using fine dissecting scissors and transferred to gentleMACS C-tubes (Miltenyi Biotec, Cat-Nr:130-093-237) containing enzymatic digestion solution according to manufacturer’s protocol for the Neonatal Heart Dissociation Kit (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2023Quote: ... Human tumor cells were isolated from primary human omental metastases with human tumor cell isolation kit (Miltenyi Biotec, Cat# 130-108-339) using MACS® cell separation technology following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... NeutrAvidin-functionalized devices were incubated with either (1) 2 μg/ml biotinylated mouse anti-human anti-SSEA-5 antibody (Miltenyi Biotec, Bergisch Gladbach, Germany) (2 ...
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Stability and heterogeneity in the anti-microbiota reactivity of human milk-derived Immunoglobulin AbioRxiv - Immunology 2023Quote: ... APC Anti-Human IgA [Anti-Human IgA APC (Miltenyi Biotec clone REA1014, (1:50)] ...
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bioRxiv - Immunology 2022Quote: ... Cells were labeled for at least 20 minutes at 4 ° C and CD14+ cells were isolated by magnetic column (Miltenyi Biotec, catalog# 130-042-201).
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bioRxiv - Developmental Biology 2022Quote: ... The lungs were incubated for 20min at 37 ◦C under continuous rotation and then run on the gentleMACS m-Lung-2 program on the gentleMACS (Miltenyi Biotec, Cat #130-095-937). This was followed by brief centrifugation and the passage of cells through the 70-uM filter ...
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bioRxiv - Physiology 2023Quote: ... Hearts were cut in small pieces and dissociated at 37°C with the commercially available enzyme mix (Neonatal Heart Dissociation Kit, mouse and rat, Miltenyi Biotec GmbH; # 130-098-373) followed by tissue homogenization with the genteMACS™ Dissociator (Miltenyi Biotec GmbH ...
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bioRxiv - Genomics 2023Quote: ... the tissue was chopped into small pieces with scissors and then transferred along with the lysing buffer into a gentleMACS C tube (Miltenyi Biotec, Cat. 130-096-334). An additional 1 ml of lysing buffer was added into the C tube to make a final volume of 2 ml ...
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bioRxiv - Developmental Biology 2023Quote: ... The lungs were incubated for 20min at 37 ◦C under continuous rotation and then run on the gentleMACS m-Lung-2 program on the gentleMACS (Miltenyi Biotec, Cat #130-095-937). This was followed by brief centrifugation and the passage of cells through the 70-uM filter ...
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bioRxiv - Neuroscience 2023Quote: ... frozen brain sections were thawed in ice cold Nuclei Extraction Buffer (cat#: 130-128-024, Miltenyi) in gentleMACS™ C-Tubes (cat#: 130-093-237, Miltenyi Biotec, Bergisch Gladbach, Germany), followed by automated gentleMACS™ Octo dissociation (cat# ...
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bioRxiv - Cancer Biology 2022Quote: ... Human CD34-PE (Miltenyi Biotec);and Human CD33-APC-Cy7 (Abcam).
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bioRxiv - Microbiology 2020Quote: ... αhuman-IgG-PE (Miltenyi Biotec). αHis-PE (Miltenyi Biotec) ...
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bioRxiv - Immunology 2023Quote: ... human and mouse (Miltenyi Biotec). Anti-human CD41 directly conjugated to Alexa Fluor 488 (Clone ...
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bioRxiv - Immunology 2024Quote: ... human FcR Blocking (Miltenyi Biotec), and 5% mouse serum (Sigma Aldrich) ...