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Citations for Miltenyi Biotec :
351 - 400 of 408 citations for Corning Hygromycin B Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... and 1:10 50nm-magnetic-bead antibody solution against rat/mouse CD90.1 (Thy1.1) (Miltenyi Biotec, 120-094-523). Nerve fibroblasts express the marker CD90.1 ...
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bioRxiv - Cancer Biology 2021Quote: ... red blood cells were removed using the Red Blood Cell Lysis Solution according to the manufacturer’s instructions (Miltenyi Biotec). Cells were then washed ...
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bioRxiv - Developmental Biology 2020Quote: ... Disaggregated blastomeres in solution were incubated with 10 μl of magnetic microbeads conjugated to mouse anti-FITC (Miltenyi Biotec) for 15 min on ice ...
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bioRxiv - Immunology 2021Quote: ... A consecutive step to remove debris from the blood was performed by using the Debris Removal Solution (Miltenyi Biotec) according to the manufacturer protocol ...
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bioRxiv - Immunology 2023Quote: ... Leukocytes were isolated from whole blood by hypotonic lysis of erythrocytes using Red Blood Cell Lysis Solution (Miltenyi Biotec) followed by ice cold PBS/1% BSA/2mM EDTA washes ...
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bioRxiv - Genomics 2023Quote: ... Red blood cells (RBC) were lysed using Red Blood Cell Lysis Solution (catalog no. 130-094-183, Miltenyi BIotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... tissues were either freshly processed or transiently stored overnight MACS Tissue Storage solution (Cat. #130-100-008, Miltenyi Biotec) at 4 degree Celsius (in case of delayed surgeries ...
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bioRxiv - Genomics 2023Quote: ... pre-coated in 1 mM EDTA and deposited into a 5 mL ice-cold of PEB buffer (PBS, pH 7.2, 0.5% BSA, 2 mM EDTA; dilution of MACS BSA Stock Solution [Miltenyi Biotec, 130-091-376] 1:20 with autoMACS® Rinsing Solution [Miltenyi Biotec ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were resuspended in 100 µl bead solution from the Dead Cell Removal Kit (Miltenyi Biotec, #130-090-101) and incubated for 15 min in the dark ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples from Sahlgrenska University Hospital were collected in ice-cold MACS Tissue Storage Solution (Miltenyi Biotec, #130-100-008) and shipped overnight to Uppsala ...
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bioRxiv - Cancer Biology 2024Quote: ... the tissues of primary GBM tumors (n=12) from patients were collected into MACS Tissue Storage Solution (Miltenyi Biotec) by surgical excision and next ...
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bioRxiv - Cancer Biology 2024Quote: ... Subsequent debris removal was then performed using Debris removal solution according to manufacturer protocol (Miltenyi Biotec, #130-109-398) to obtain a clean sample of highly viable cells ...
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bioRxiv - Bioengineering 2021Quote: ... and the cell pellet was gently re-suspended in Red Blood Cell Lysis Solution (freshly prepared according to manufacturer’s instruction; 130-094-183; Miltenyi Biotec B.V ...
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bioRxiv - Cell Biology 2020Quote: ... Dissected muscles were minced in collagenase/dispase solution followed by dissociation using the gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec). Satellite cells were sorted by gating a mononuclear cell population of α7-INTEGRIN+ ...
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bioRxiv - Developmental Biology 2022Quote: ... Each purified hepatocyte pellet was resuspended in 5ml of red blood cell (RBC) lysis solution (Miltenyi Biotec 130-094-183) and incubated at room temperature for 10 mins ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were then resuspended in wash buffer and incubated in MACS human FcR blocking solution (20μl/sample, cat nb. 130-059-901, Miltenyi Biotec) and primary antibodies against CD45-FITC (12μl/sample ...
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bioRxiv - Immunology 2021Quote: ... the samples were gently incubated with a proprietary enzymatic solution in C-tubes (Miltenyi Biotec, Cat. No.: 130-096-334) at 37°C for at least 30 minutes using a rotator ...
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bioRxiv - Microbiology 2023Quote: ... lungs and spleens were collected in 5 ml MACS Tissue storage solution (Cat: 130-100-008; Miltenyi Biotec, Gaithersburg, MD) followed by storage at 4 °C until processing ...
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bioRxiv - Immunology 2023Quote: ... tumour suspensions were mashed through a 70 μm filter and live cells were enriched using debris removal solution (Miltenyi Biotec). For obtaining single cell suspensions for the KM macrophage datasets ...
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bioRxiv - Cancer Biology 2024Quote: ... SmartStrainers were used to filter debris and erythrocyte lysis was performed with a Red Blood Cell Lysis Solution (Miltenyi Biotec). Cells were counted and frozen in MACS Freezing Solution and store at – 80°C.
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bioRxiv - Cancer Biology 2022Quote: ... each blood sample was diluted in a 1 to 1 ratio with the HBSS buffer solution and incubated with 10% EpCAM MicroBeads (Miltenyi Biotec) with shaking at room temperature for an hour ...
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bioRxiv - Molecular Biology 2020Quote: ... dissolved in Hank’s balanced salt solution containing 50 μg/ml DNAse I and analyzed using a MACSQuant VYB flow cytometer (Miltenyi Biotec, Germany). Sytox Blue dead cell stain (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... the index tumor lesions (the most extensive with the highest Gleason score) were immediately harvested and transferred to precooled MACS tissue storage solution (Miltenyi Biotec) and shipped at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... the supernatant was removed and the cells resuspended with 400 µl of Running Buffer composed of MACS BSA stock solution (Miltenyi Biotec) 1:20 with autoMACS Rinsing Solution (Miltenyi Biotec) ...
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Single cell transcriptomics reveals the effect of PD-L1/TGF-β blockade on the tumor microenvironmentbioRxiv - Genomics 2020Quote: ... A red blood cell lysis step was performed by incubating cells for 2 minutes with 1x Red Blood Cell Lysis Solution (Miltenyi Biotec). Cells were washed again and then cell viability was measured using the Nexcelom Cellometer K2 Fluorescent Viability Cell Counter and the ViaStain AOPI Staining Solution (Nexcelom) ...
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bioRxiv - Cell Biology 2021Quote: ... whole bone marrow (WBM) cells isolated by crushing femur and tibia from each mouse were incubated with 1x RBC Lysis Solution (Miltenyi Biotec) to eliminate red blood cells ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies were added at a dilution of 1:100 in autoMACs Rinsing Solution (Cat. No. 130-091-222, Miltenyi Biotec) containing 0.5% BSA at 4 °C for 1 hour ...
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bioRxiv - Immunology 2021Quote: Mouse spleens and lymph nodes were removed from RNAlater solution and transferred to gentleMACS M tubes (Miltenyi Biotec 130-093-236) and 2 ml microtubes (OMNI 19-629-3 ...
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bioRxiv - Neuroscience 2023Quote: ... were sacrificed at E14.5 and brain cortices dissected under a stereomicroscope in ice-cold Hanks’ Balanced Salt Solution (HBSS) and enzymatically dissociated using a Neural Tissue Dissociation Kit (Miltenyi Biotec) following manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... containing enzymatic digestion solution according to manufacturer’s protocol for the Neonatal Heart Dissociation Kit (Miltenyi Biotec, Cat-Nr: 130-098-373). Two mouse hearts were processed in separate C-tubes at the same time for all experiments presented in this manuscript ...
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bioRxiv - Systems Biology 2024Quote: ... we first processed a portion of the cell harvest solution using the dead cell removal kit (Miltenyi Biotec 130-090-101) to remove any cell debris to avoid any free RNA from lysed cells from getting mixed in with mRNA to be extracted from live cells ...
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bioRxiv - Cell Biology 2023Quote: ... Freshly tumor (n = 8) and adjacent normal lung samples (n = 8) were delivered within MACS® Tissue Storage Solution (Miltenyi Biotec) on ice directly from the operating room to the laboratory after frozen pathology (1-2h).
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bioRxiv - Microbiology 2023Quote: ... containing 2.35 mL of a digestion solution and homogenized on a GentleMACS Octo Dissociator (Miltenyi Biotec, #130-096-427, program 37C_m_LPDK_1). Homogenates were resuspended in 5 mL PB Buffer (phosphate-buffered saline (PBS) ...
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bioRxiv - Immunology 2022Quote: ... Further disaggregation of iCCA tissue into a single-cell solution for sequencing was completed using the MACS tumor dissociation kit (Miltenyi Biotec) with the standard tough tumor protocol ...
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bioRxiv - Cell Biology 2022Quote: ... The cell solution was then filtered through a 70 μm cell strainer and separated with magnetic beads (mouse CD31, Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... The samples were then centrifuged for 10 min at 3000 x g in the presence of debris removal solution (Miltenyi Biotec) and 10 min at 1000 x g in cooled PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... After dissociation the cell suspension was filtered through a 40 µm strainer and treated with red blood cell lysis solution (Miltenyi Biotec). Lastly ...
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bioRxiv - Microbiology 2024Quote: ... the mice were sacrificed and their lungs were harvested and homogenized in lysis solution (ZR Fungal/Bacterial DNA MiniPrepTM, Epigenetics) using a gentleMACS dissociator (Miltenyi Biotec). The homogenates were clarified by centrifugation and the cytokine content of the supernatant was determined using a Luminex Multiplex Assay ...
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bioRxiv - Bioengineering 2024Quote: ... the cells were subcultivated by incubating the cells for 5 minutes at room temperature with StemMACS Passaging Solution XF (Miltenyi Biotec). Subsequently ...
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bioRxiv - Cell Biology 2024Quote: ... The solution was then spun down at 300g for 7 minutes and the pellet was either resuspended in wash buffer for downstream endothelial cell isolation or was resuspended in 1ml StemMACSTM Cryo-Brew solution (130-109-558, Miltenyi Biotec) and frozen until later use ...
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bioRxiv - Bioengineering 2021Quote: ... MACS buffer was prepared freshly before each isolation by diluting MACS BSA Stock Solution (130-091-376; Miltenyi Biotec B.V. & Co. KG) 1:20 in autoMACS Rinsing Solution (130-091-222 ...
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bioRxiv - Neuroscience 2021Quote: ... the pellet was resuspended in MACS buffer and the solution was passed through LS columns (Miltenyi Biotec, Cat. No. 130-042-401). The cells were centrifuged at 300g for 15 min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... The resulting cell suspensions were filtered through a 70 μm strainer and centrifuged in a density gradient using debris removal solution (#130-109-398, Miltenyi Biotec, Germany) according to the manufacturer’s protocol to remove myelin and cell debris ...
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bioRxiv - Cell Biology 2022Quote: BAL samples from silica beads and silica treated mice were treated for 10 min with red blood cell lysis solution (Miltenyi Biotec, 170080033) and washed with PBS by centrifugation at 300 g for 10 min ...
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bioRxiv - Cancer Biology 2022Quote: ... The suspension was filtered through a 70 μm strainer and subjected to a density gradient centrifugation using debris removal solution (#130-109-398, Miltenyi Biotec, Germany) according to manufacturer’s protocol to remove myelin and cell debris ...
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bioRxiv - Cancer Biology 2023Quote: ... RBCs and debris of dead cells in single cell suspensions were respectively removed using Red Blood Cell Lysis Solution (10x) Kit (Miltenyi Biotec, Germany) and Dead Cell Removal Kit (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2024Quote: The biopsy and surgical samples used to prepare the single cell suspension were immediately put into a tissue preservation solution (Miltenyi Biotec, Germany) and transported to the field laboratory in an ice bath for immediate preparation of the single cell suspension ...
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bioRxiv - Immunology 2024Quote: ... Both ∼1-2 mm tumor pieces and whole spleens were placed in an ice-cold Tissue Storage Solution (#130-100-008) until being transferred into gentleMACS™ C Tubes (130-093-237; Miltenyi Biotec) loaded with the appropriate kit reagents and enzymes ...
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bioRxiv - Cancer Biology 2024Quote: ... 1-2 mm tumour pieces were placed in an ice-cold Tissue Storage Solution (#130-100-008) before transferred into gentleMACS™ C Tubes (Miltenyi Biotec) containing the appropriate kit reagents and enzymes ...
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bioRxiv - Neuroscience 2023Quote: Human tumor tissue samples were acquired during neurosurgical biopsy or open resection and stored in Tissue Storage Solution (Miltenyi Biotec, 130-100-008) for 2-30 hours until further processing for scRadiotracing ...