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Citations for Miltenyi Biotec :
351 - 400 of 400 citations for Anion Exchange Membranes Thickness 10 13 µm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... the suspension was counted and incubated with Anti-O4 MicroBeads at a concentration of 10 uL of beads per 1×107 cells (Miltenyi Biotec). After incubation ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were resuspended in 1 ml 10% FBS in PBS and stained with 6 μg anti-mouse Ter119-PE antibody (Miltenyi Biotec) per 108 cells for 20 minutes at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... Peritoneal macrophages were isolated from adult (8-10 weeks old) female mice using Peritoneum Macrophage Isolation Kit (130-110-434, Miltenyi Biotec). Isolated cells were cultured in complete RPMI+GlutaMAX (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... followed by a 15 min incubation with 10 µL microbeads mixture containing antibodies directed against CD140 (130-101-502, Miltenyi Biotec), NG2 (130-097-170 ...
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bioRxiv - Neuroscience 2023Quote: ... The pelleted MVs were incubated with 10 μl of Biotin-conjugated anti-CD105 (Miltenyi Biotec cat# 130-094-916, RRID: AB_10828348) followed by adding 10 μl of anti-Biotin microbeads (MiltenyiBiotec cat# 130-094-256 ...
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bioRxiv - Developmental Biology 2023Quote: ... The cells were then strained through a 50 µm cell strainer and 1 million cells in 100 µL were incubated with 10 µL of Fc receptor block (Miltenyi Biotec) for 10 minutes on ice ...
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bioRxiv - Biophysics 2023Quote: ... antibodies in T cell media (RPMI 10 % FBS, 2 mM L-Glutamine, 1 % Penicillin-Streptomycin) with IL-15 and IL-7 (Miltenyi Biotec, 10 ng/ml each ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were washed twice and resuspended in 1mL of sorting buffer (up to 10⁸ cells) prior to magnetic separation on LS columns and the MACS Separator (Miltenyi Biotec). Columns were rinsed with 0.5mL of MACS buffer prior to loading the cell suspension ...
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bioRxiv - Cancer Biology 2023Quote: ... The samples were then centrifuged for 10 min at 3000 x g in the presence of debris removal solution (Miltenyi Biotec) and 10 min at 1000 x g in cooled PBS ...
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bioRxiv - Synthetic Biology 2023Quote: ... supplemented with 10% Human AB serum (Gemini Bio Products) with the addition of 100 U/ml human IL-2 IS (Miltenyi Biotec), 1 ng/ml IL-15 (Miltenyi Biotec) ...
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Novel RNA-binding protein YebC enhances translation of proline-rich amino acid stretches in bacteriabioRxiv - Molecular Biology 2024Quote: ... Then the medium was replaced by RPMI supplemented with 10% human serum type AB and 100ng/mL GM-CSF (Miltenyi Biotec). The following day ...
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bioRxiv - Immunology 2024Quote: ... lungs of infected mice were harvested and homogenized in 1 mL of minimal essential media with 10% bovine serum albumin using a gentleMACS C Tube (Miltenyi Biotec). Subsequently ...
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bioRxiv - Immunology 2024Quote: ... Pieces of approximately 1 cm2 were placed in 10 mL PBS containing 2% FBS and homogenized for 10min at room temperature using a gentleMACS dissociator (Miltenyi Biotec). Homogenized tissue was filtered through a 70 μm cell strainer and pooled ...
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bioRxiv - Neuroscience 2024Quote: ... for 10 min at 4°C and incubated with anti-mouse CD11b-PE (1:50 dilution; 130-113-806, Miltenyi Biotec) and anti-mouse CD45-APC (1.15:100 dilution ...
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bioRxiv - Immunology 2024Quote: ... Cells were resuspended in 900 µl/107 of isolation buffer containing 0.5% Bovine Serum Albumin (BSA) and 2 mM EDTA in PBS and 10 µl/1x107 of mouse anti- Ly6G MicroBeads (Miltenyi Biotec) and incubated at 4°C for 10 mins ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were washed with PBS to remove excess AO and the cell pellet was resuspended in 500 μl PBS for flow cytometry analysis with the MACSQuant Analyzer 10 (Miltenyi Biotec) flow cytometer ...
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bioRxiv - Cancer Biology 2024Quote: ... TILs were either transduced and/or expanded in complete X-Vivo15 medium supplemented with 50 ng/ml IL-15 and 10 ng/ml IL-7 (Miltenyi Biotec).49
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bioRxiv - Cancer Biology 2024Quote: Fresh spleen was collected in 3mL complete medium (CM; DMEM + 10%FBS + 1% peniciline/streptomycin) in a homogenization tube (gentleMACSTM C Tubes, Miltenyi Biotec). Spleen was dissociated using tissue dissociator gentleMACS Octo (gentleMACSTM ...
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bioRxiv - Microbiology 2022Quote: ... stained samples were diluted 10-fold in 1x PBS and cell concentrations as well as green fluorescence intensities were recorded on a MACSQuant Analyzer 10 (Miltenyi Biotec, Germany).
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bioRxiv - Microbiology 2023Quote: Parasites were enriched from ∼100 mL of culture containing synchronized schizonts (∼36-44 h post-invasion) at ∼10% parasitemia using a SuperMACS™ II Separator and a D Column (Miltenyi Biotec). Numbers of eluted cells were determined with a hemocytometer and parasitemia was calculated from a Giemsa-stained smear ...
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bioRxiv - Cancer Biology 2023Quote: Human AML and CB cells were seeded for 10-12 days in methylcellulose-based semi-solid medium (StemMACS HSC-CFU Media complete with EPO, Miltenyi Biotec, Germany) for assessment of the frequency of colony formation ...
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bioRxiv - Cell Biology 2022Quote: BAL samples from silica beads and silica treated mice were treated for 10 min with red blood cell lysis solution (Miltenyi Biotec, 170080033) and washed with PBS by centrifugation at 300 g for 10 min ...
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bioRxiv - Cancer Biology 2023Quote: Splenic B cells from mice immunized with SRBC for 10 days were pre-enriched by B220 MicroBeads (Miltenyi Biotec; 130-049-501) followed by flow sorting ...
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bioRxiv - Immunology 2024Quote: ... Cells were incubated at 37°C for 30 minutes in the presence or absence of 10 ng/ml human recombinant IL-6 (Miltenyi Biotec, Germany) or IL-23 (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2024Quote: ... 48h or 72h after platelet addition the number of PI - positive cells was evaluated by a MACSQuant® Analyzer 10 (Miltenyi Biotec). In indicated experiments ...
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bioRxiv - Neuroscience 2020Quote: ... and homogenized mechanically into respective Gentle MACS C tubes containing 50 µL enzyme P + 1900 µL buffer Z and 20 µL of Buffer Y + 10 µL of Enzyme A (Miltenyi Biotec, Auburn, CA). Samples were passed through a 70 µm cell strainer (Millipore ...
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bioRxiv - Neuroscience 2022Quote: ... Red Blood Cell Remover Solution was made by mixing 125 μL of Red Blood Cell Lysis Solution 10× (Miltenyi Biotec, # 130-094-183) with 1125 μL of H2O ...
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bioRxiv - Neuroscience 2022Quote: ... The remaining pellet was resuspended in 1.25 mL of Red Blood Cell Remover Solution and incubated for 10 minutes at 4 °C before the addition of 12 mL of 0.5 % bovine serum albumin (BSA, Miltenyi Biotec, # 130-091-376) diluted in DPBS ...
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Autocrine Sfrp1 inhibits lung fibroblast invasion during transition to injury induced myofibroblastsbioRxiv - Cell Biology 2022Quote: ... the labeled cells were incubated with 100 µl per 10 million cells of MACS dead cell removal beads (Miltenyi Biotec, 130-090-101) and 20 µl of each microbeads specific against AlexaFluor647 (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: The left lung was weighed and homogenized in 1 mL of RPMI supplemented with 10% FBS in GentleMACS™ M tube using GentleMACS™ Dissociator (Miltenyi Biotec) with RNA 2.01 program setting ...
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bioRxiv - Developmental Biology 2024Quote: ... in 200 μl of myeloid medium (MM) (DMEM with high glucose and L-glutamine, 10% FBS, and 1% Pen-Strep, supplemented with 50ng/ml Tpo (Miltenyi Biotec, Auburn, CA), 5ng/ml SCF ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatant was removed and the SVF resuspended in 10 ml room temperature plain DMEM followed by additional filtering through a 30 μm mesh filter (Miltenyi Biotec # 130-041-407) and subsequent centrifugation at 700 g for 5 min ...
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bioRxiv - Immunology 2021Quote: ... Erythrocyte lysis was terminated by washing the cells in the 10-fold volume of FACS buffer (autoMACS Running Buffer, Miltenyi Biotec, Bergisch Gladbach, Germany) and passed through a 30 µm cell strainer (BD Biosciences ...
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bioRxiv - Neuroscience 2021Quote: ... a 5 µl volume of phosphate-buffered PBS (PBS) containing 10 μg/kg/injection of recombinant mouse IL-1β (Miltenyi Biotec, Bergisch Gladbach, Germany) or of PBS alone (control ...
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bioRxiv - Bioengineering 2021Quote: ... CD3/CD28-mediated activation of T cells was conducted by using 10 μl of T cell TransActTM (130-111-160; Miltenyi Biotec B.V. & Co. KG) according to manufacturer’s instructions (T cell activation medium) ...
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bioRxiv - Genomics 2020Quote: ... Cells were washed with cold PBS/10% FBS and resuspended in 10 μl of cold PBS/10% FBS per 106 cells and stained with a 1/100 dilution of anti-Ter119-PE antibody (Miltenyi Biotec: 130-102-336) at 4 °C for 20 minutes ...
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bioRxiv - Genomics 2022Quote: ... Cells were then collected and incubated with antibodies directed against Glast1 (Allophycocyanin-conjugated, ACSA-1, 1:10 dilution) (Miltenyi Biotec, Cat#: 130-098-803), 1:100 dilution of Anti-mouse CD133 antibody conjugated to PE (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... Fluorescent intensity of 10 000 cells was detected by flow cytometry on a FACS instrument (MACSQuant®VYB, Miltenyi Biotec, San Diego, CA, USA). Data were analyzed using FlowJo software (FlowJo LLC ...
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bioRxiv - Bioengineering 2024Quote: ... Canada) for 48 hours or T-cell TransAct (10 uL for each mL of culture, Cat # 130-111-160, Miltenyi Biotec, Bergisch Gladbach, Germany) for 72 hours respectively.
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bioRxiv - Cell Biology 2022Quote: One million cells suspended in 90 μl of staining buffer (eBiosciences, 00-4222-26) were incubated with 10 μl FcBlock (Miltenyi Biotec, 130-092-575) to reduce nonspecific antibody binding ...
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bioRxiv - Genomics 2024Quote: ... The cells were centrifuged at 300 x g for 10 minutes and further submitted to live cell sorting with the Magnetic Dead Cell Removal Kit (Miltenyi Biotec, 130-090-101), according to the manufacturer ...
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bioRxiv - Immunology 2024Quote: ... AVX/COVID-12 or 30 nM of a mixture of 10-15 amino acid long peptides derived from the SARS-CoV-2 spike protein (PepTivator, Miltenyi Biotec, North Rhine-Westphalia, Germany). In parallel ...
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bioRxiv - Bioengineering 2023Quote: ... Media supplements tested were FGF-2 at a concentration of 10 ng/ml (Human FGF-2 IS premium grade, MACS Miltenyi Biotec, Germany, 130-104-922), EGF at a concentration of 10 ng/ml (Recombinant Human EGF ...
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bioRxiv - Cancer Biology 2024Quote: ... TL-1 cells were cultured in RPMI-1640 with 10% FBS and supplemented with 200 U/mL IL-2 (Miltenyi Biotec cat # 130-097-743) and NKL cells were cultured in RPMI-1640 with 10% FBS and supplemented with 100 U/mL IL-2.
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bioRxiv - Genetics 2022Quote: ... 150 µl of 1M HEPES and 0.75 µl of 10 mg/ml DNAse I) and dissociated in gentleMACS Dissociator (Miltenyi Biotec Inc., cat. no. 130-093-235) using the Multi_E_01.01 program ...
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bioRxiv - Neuroscience 2024Quote: The telencephalon of the 10-month-old fish were dissected in ice-cold PBS and directly dissociated with Neural Tissue Dissociation Kit (Miltenyi Biotec, Cat. No. 130-092-628) as described previously24 ...
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bioRxiv - Immunology 2024Quote: ... or normal tissue were placed in 50 ml Falcon tubes containing 7-10 ml of MACS® Tissue Storage Solution (Miltenyi Biotec, cat. 130-100-008) and stored at room temperature for no longer than 2 hours ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were harvested by centrifugation at 400g for 10 min and resuspended in chilled 1X Red Blood Cell Removal Solution (Miltenyi Biotec, cat. no.130-094-183). Debris was then removed using the Debris Removal Solution (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... total splenocytes and LN cells were resuspended in 500 μl of IMDM-10 and incubated with 50 μl of CD4 MicroBeads (Miltenyi Biotec, Cat. No. 130-117-043) at 4°C for 30 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... 10-20 cortices of PN day 2-4 pups were dissociated using the MACS Neural Tissue Dissociation Kit-T (Miltenyi Biotec, Cat# 130-093-231, Auburn, CA) at 37°C (5% CO2 ...