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Citations for Miltenyi Biotec :
351 - 400 of 701 citations for Absent In Melanoma 2 AIM2 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Red blood cells and debris were removed and astrocytes magnetically labelled using ACSA-2 microbeads (Miltenyi Biotec, 130-097-678) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 femora and 2 humeri was harvested in PBS/2.5% FBS and pooled before lineage negative depletion using autoMACS Pro separator (Miltenyi Biotec). Cells were incubated with a lineage antibody cocktail (B220 ...
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bioRxiv - Immunology 2019Quote: ... All samples were washed and resuspended in 2% PFA before acquisition on a seven-color MacsQuant flow cytometer (Miltenyi Biotec). Data were analyzed using FlowJo Single Cell Analysis software.
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bioRxiv - Genetics 2020Quote: ... were washed in PBS and MACS buffer (PBS, 2 µM EDTA, 0.5% BSA) and stained with Human CD34 Microbead kit (Miltenyi Biotec) following the manufacturer’s instruction for 30 minutes (4 °C ...
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bioRxiv - Immunology 2021Quote: ... the biopsies were cut into 2-3mm pieces and dissociated using a human Tumour Dissociation Kit (Miltenyi Biotec, Surrey, UK) according to the manufacturer’s protocol with some modifications ...
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bioRxiv - Neuroscience 2022Quote: ... then astrocytes were isolated using the Miltenyi Biotec’s anti-ACSA-2 magnetic cell sorting kit and protocol (Miltenyi Biotec, 130097678). RNA was purified and concentrated with proteinase K cell digestion ...
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bioRxiv - Bioengineering 2020Quote: ... The HER2 expression level was quantified by flow cytometry using anti-ErbB-2 (CD340) Vio® Bright FITC (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... 2 to 3×108 PBMCs were taken up in 2.1 ml staining buffer (PBS, 0.5 % BSA, 2 mM EDTA) and 300 μl of anti-human CD61 and CD3 microbeads (Miltenyi Biotec) were added to remove contaminating platelets and T cells ...
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bioRxiv - Genomics 2021Quote: ... we used NS medium containing media hormone mix (MHM) medium (KOHJIN-Bio) supplemented with 2% MACS NeuroBrew-21 (Miltenyi Biotec), 20 ng/ml basic FGF (PEPROTECH) ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were resuspended in MACS buffer (PBS containing 2 mM ETDA and 2.5% FBS) supplemented with human FcR Blocking Reagent (Miltenyi Biotec) at 1:5 dilution and anti-human CD45-APC (BioLegend 304012 ...
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bioRxiv - Immunology 2022Quote: ... CD8+ SP thymocytes were isolated using the CD8a+ (Ly-2) positive selection kit per manufacturer’s instructions (Microbeads, #130-117-044; Miltenyi Biotec). Flow-through resulted in the DN population ...
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bioRxiv - Immunology 2022Quote: ... (3) Non-SARS-CoV-2 antigens: Peptide pools of the pp65 protein of human cytomegalovirus (CMV) (Miltenyi Biotec, Gladbach, GER), or HIV-1 gag peptide pools (NIH AIDS Reagent Repository ...
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bioRxiv - Neuroscience 2022Quote: ... cell pellets were resuspended in sorting buffer and incubated with ACSA-2 MicroBeads (1:10, Miltenyi Biotec, 130-097-678) for 15 min at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Hearts were mashed with pestles for 2 min and cell isolation performed according to manufacturer’s instructions (Neonatal Heart Dissociation Kit, Miltenyi Biotec). Cell suspension was centrifuged for 5 min at 600 x g and cells were resuspended in cell culture medium and plated in 22.1 mm plates ...
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bioRxiv - Bioengineering 2023Quote: ... Germany]) and activated using Dynabeads™ Human T-Activator CD3/CD28 Beads and 50 U/mL IL-2 (Miltenyi Biotec).
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bioRxiv - Neuroscience 2023Quote: Samples and relevant controls were added with 2 μL of crystallizable fragment receptor blocker (Miltenyi Biotec, cat # 130-092-575) and incubated in the dark with rotation (12min ...
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bioRxiv - Developmental Biology 2023Quote: ... Astrocytes were isolated from the remaining cell suspension using the anti-ACSA-2 microbead kit (130-097-678, Miltenyi Biotec). The cell suspension was incubated with FcR blocking reagents followed by anti-ACSA-2 microbeads and placed on a magnetic MACS separator for positive selection ...
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bioRxiv - Pathology 2023Quote: ... GMCSF/IL-9 cytokine ratios of 1:2 diluted amniotic fluid were evaluated by the cytokine bead array (Miltenyi Biotec MACSPlex Cytokine 12 Kit ...
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bioRxiv - Immunology 2024Quote: ... MP1 and SARS-CoV-2 Prot_S (SPIKE) (peptides pools of 15 amino acids length with 11 amino acid overlap, Miltenyi Biotec) in 5% human serum RPMI medium in the presence of 1µg/ml anti-CD40 (HB14 ...
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bioRxiv - Systems Biology 2024Quote: ... tumor was cut into 1-2 mm pieces and transferred to a gentleMACS C Tube (Miltenyi Biotec #130-093-237) containing 4.7 mL of sterile media (Knockout DMEM/F12) ...
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bioRxiv - Pathology 2024Quote: ... The cells were resuspended in 200 μl of PBS containing 0.5% BSA and 2 mM EDTA and incubated with 15 μl of mouse FcR blocking reagent (Miltenyi Biotec) on ice for 15 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were washed with 10% FBS in PBS and resuspended in 135 ml cold PBE buffer (PBS with 0.5%BSA and 2 mM EDTA) with 15 ml Ter119-PE microbeads (Miltenyi Biotec) per 107 cells ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were resuspended in MACS buffer (PBS, 0.5% BSA, 2 mM EDTA) for CD34+ magnetic selection using CD34 human MicroBeads (Miltenyi Biotec; cat ...
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bioRxiv - Immunology 2024Quote: ... resuspended at 4 mio cells/ml and then cultured with HLA class I-enhanced neurons (see Human-iPSC-derived neuron preparation section above) at a density of 1.2mio cells/cm2 with IL-2 (1000UI/ml, Miltenyi Biotec) and a CD28 agonist antibody (5ug/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-CD28 antibody (15E8; 0.5µg/mL) (Miltenyi Biotec, Cat# 130-093-387 and 130-093-375) and IL-2 (100 U/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-CD28 antibody (15E8; 0.5µg/mL) (Miltenyi Biotec, Cat# 130- 093-387 and 130-093-375), and IL-2 (100 U/mL ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were then trypsinized and incubated with anti-CD146 antibody coated microbeads (Miltenyi Biotec Inc.) for 15 mins at 4°C to obtain the CD140b+CD146+ cells for subsequent experiments ...
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bioRxiv - Immunology 2022Quote: ... MAIT cell staining (1 ×106 PBMC) was performed using specific surface monoclonal antibodies (All Miltenyi Biotec) namely ...
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bioRxiv - Microbiology 2022Quote: ... Bacteria were stained with PE Anti-human IgA antibodies (1:100; Miltenyi Biotec clone IS11-8E10) for 30 min on ice ...
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bioRxiv - Neuroscience 2020Quote: ... where Magnetic isolation (MACS) was performed with anti-NG2 antibody conjugated with magnetic beads (Miltenyi Biotec). For the P9 time-point ...
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bioRxiv - Microbiology 2021Quote: ... The antibodies used for FACS analysis were APC-conjugated anti-CD163 (clone REA812, MACS Miltenyi Biotec), PE-conjugated mouse anti-HLA-DR (clone LN3 ...
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bioRxiv - Immunology 2022Quote: Separation was performed using anti-CD3 antibodies conjugated to ferromagnetic microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany) and directed through a cell separation column containing a magnetic field (Miltenyi Biotec) ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were stained with the anti-CD326 (EpCAM) magnetic microbeads antibody (Miltenyi Biotec, 130-105-958) for 40 min on ice ...
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bioRxiv - Cancer Biology 2019Quote: ... we also used CD3-PE to specifically identify lymphocytes (human antibody, clone BW264/56, Miltenyi Biotec). For all the samples ...
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bioRxiv - Cancer Biology 2020Quote: ... Merkel cells were stained with anti-PSA-NCAM-PE antibody (Miltenyi Biotec, Cat. 130-117-394) or with mouse IgM-PE isotype control (Miltenyi Biotec ...
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bioRxiv - Plant Biology 2021Quote: ... H-tagged proteins were detected using the anti-HA HRP-coupled antibody (mouse monoclonal, Miltenyi Biotec) diluted 1:5000 (v/v) ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins were subsequently detected with horseradish peroxidase (HRP)-conjugated anti-GFP antibody (1:5,000) (Miltenyi Biotec) for 1 hr at room temperature with gentle shaking ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were subsequently detected with horseradish peroxidase (HRP)-conjugated anti-GFP antibody (1:5,000) (Miltenyi Biotec) or HRP-conjugated anti-c-myc antibody (1:5,000 ...
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bioRxiv - Microbiology 2023Quote: ... and 20 minute incubation with primary antibody cocktail (1:50 anti-CD45.1-PE, REAfinity [Miltenyi Biotec, Germany]/1:50 anti-CD45.2-VioBlue ...
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bioRxiv - Cell Biology 2023Quote: ... The pellets were resuspended in 50 μL of pooled REAfinity antibodies (Miltenyi Biotec, 1:50 dilution) for 40 minutes on ice ...
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bioRxiv - Bioengineering 2023Quote: ... streptavidin magnetic beads were first conjugated with the desired biotinylated antibody (anti-CD63, biotin; Miltenyi Biotec) and allowed to incubate with the sample stirring overnight ...
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bioRxiv - Neuroscience 2024Quote: Purified single-cells suspensions were stained using antibodies against mouse CD45 (Miltenyi Biotec; 130-117-498), SCA1 (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2020Quote: ... and stimulated for 18 h with IL-2 (20 ng/ml; National Cancer Institute Preclinical Repository) and/or IL-12 (10 ng/ml; Miltenyi Biotec) cytokines ...
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bioRxiv - Immunology 2021Quote: ... the cells were resuspended in PBS containing 2% FCS and 2mM EDTA and stained with the extracellular mix using APC-Vio770 anti-CD14 (clone REA599) from Miltenyi Biotec and used at 1/100 ...
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bioRxiv - Plant Biology 2020Quote: ... 1% (v/v) Nonidet P-40) and incubated for 2 h with anti-green fluorescent protein (GFP) magnetic beads (MACS / Miltenyi Biotec) at 4 °C under rotation ...
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bioRxiv - Immunology 2022Quote: ... lungs of SARS-CoV-2 infected mice were collected and homogenized with gentle MACS M tubes (Miltenyi Biotec, 130-093-236) in a gentle MACS dissociator (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2020Quote: ... Astrocytes and microglia in the cell suspension were removed by using anti-ACSA-2 micro beads (130-097-679, Miltenyi Biotec) and anti-CD11b micro beads (130-049-601 ...
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bioRxiv - Immunology 2019Quote: ... T cells were isolated by magnetic selection of CD4 or CD8 BM cells or splenocytes using the Miltneyi MACS system (QuadroMACS Separator, LS columns, CD4 [L3T4] MicroBeads, CD8a [Ly-2] MicroBeads; Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... whole lungs from mice bearing MDA-LM2 macrometastases (3 weeks post tail vein injection) were harvested and lysed in 1 ml Cell Lysis Buffer 2 (R&D) using the gentleMACS Dissociator (Miltenyi Biotec). As a control ...
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bioRxiv - Immunology 2021Quote: ... were co-transferred with CD3+ cell-depleted autologous PBMCs (2 × 106) isolated using CD3 Microbeads and the LD Column (Miltenyi Biotec) into 5- to 7-week-old female mice ...