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Citations for Miltenyi Biotec :
351 - 400 of 927 citations for 7 Bromo 2 chloromethyl 4H pyrido 1 2 a pyrimidin 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... and cells were washed and labeled with the following antibodies for 30 min at 4 °C: rat anti-CD45 PerCP/Cyanine5.5 (1:100, catalog no. 130-102-469; Miltenyi Biotec); rat anti-SiglecH PE (1:100 ...
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bioRxiv - Immunology 2020Quote: ... in combination with or without recombinant interleukin-4 (IL-4, Miltenyi Biotec, catalogue number: 130-094-061) for 24h ...
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bioRxiv - Immunology 2021Quote: ... The isolated T cells were then stimulated with IL-7 and IL-15 (Miltenyi Biotec, Bergisch Gladbach, Germany) at a concentration of 25μg/2L bag of TexMACS media with 3% human AB serum (Innovative Research ...
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bioRxiv - Biophysics 2024Quote: ... Cells were cultured in TexMACS media with IL-7 and IL-15 (Miltenyi Biotec; North Rhine-Westphalia, Germany). Cells were then cryopreserved until experimentation ...
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bioRxiv - Neuroscience 2020Quote: ... Resuspended total EVs were incubated with 4 μg of anti-GLAST (i.e. antibody against the astrocyte cell surface antigen-1; RRID: AB_2733473; Miltenyi Biotec, Auburn, CA), anti-human CD171 (i.e ...
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bioRxiv - Neuroscience 2019Quote: ... or left untransduced for 4 days prior to lysis in mitochondria isolation kit lysis buffer (1×107 cells/mL; Miltenyi Biotec) with protease inhibitors (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: Cryopreserved grafts were thawed and stained using standard procedures (10 min, 4°C) with the surface antibody CD34-PE-Vio770 (clone AC136, lot# 5180718070, dilution 1:50, Miltenyi Biotec) and DAPI (Sigma-Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... spleens were harvested and single cell suspensions were incubated with APC-conjugated I-Ab Mtb Ag85b280-294 tetramer (provided by National Institutes of Health tetramer core facility) for 1 hour at 4°C and tetramer-positive cells were enriched using anti-APC Micro-Beads (Miltenyi Biotec). CD19−CD11c−CD11b−F4/80−CD8a− WT (CD45.1+CD45.2+ ...
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bioRxiv - Cell Biology 2022Quote: ... JAM-1 and CD45 (Fig. 3, Supplementary Table 4) for 15 min prior to analysis on a MACSQuant10 Flow Analyzer (Miltenyi Biotec). To determine proportions of SCs and FAPs (Fig ...
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bioRxiv - Neuroscience 2024Quote: ... for 10 min at 4°C and incubated with anti-mouse CD11b-PE (1:50 dilution; 130-113-806, Miltenyi Biotec) and anti-mouse CD45-APC (1.15:100 dilution ...
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bioRxiv - Immunology 2020Quote: ... Th2: 20ng/ml rmIL-4 (Miltenyi Biotec). Intracellular cytokine staining was performed after incubation for 4-6h with Cell Stimulation cocktail plus Golgi transport inhibitors (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... 10 ng/mL BMP-4 (Miltenyi Biotec), and 10 µM Y-27632 (Abcam ...
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bioRxiv - Immunology 2024Quote: ... BDCA-4-magnetic beads (MACS Miltenyi Biotec), LS Columns (MACS Miltenyi Biotec ...
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bioRxiv - Immunology 2020Quote: C1 and C4-plasma generated cells were isolated after 7 d of culture using human CD14 Microbeads (Miltenyi Biotec). Isolated CD14+ cells were validated using flow cytometry (CD14-FITC ...
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bioRxiv - Molecular Biology 2022Quote: ... Plated neurospheres were maintained for 7 days on N2B27 basal medium supplemented with Dual Smad inhibitors (Miltenyi Biotec (SB), Tocris (DM)) ...
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bioRxiv - Neuroscience 2024Quote: ... The cell pellet was then resuspended in 100 µl of cold D-PBS containing fluorochrome-conjugated antibodies recognizing mouse CD11b and ACSA-2 (Miltenyi Biotec, 130-113-810 and 130-116-247) in a 1:100 dilution and incubated for 10 min at 4°C in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... Nuclei pellets were washed tree times with one filtration step by MACS pre-separation filter 20μm (Miltenyi Biotec). Nuclei pellets were resuspended in 100 μL of staining buffer (DPBS BSA 2% ...
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bioRxiv - Neuroscience 2024Quote: ... The anti-TOMM20 antibody was biotinylated via Miltenyi One-Step Antibody Biotinylation Kit (Miltenyi Biotec, 130-093-385) following the manufacture’s protocol ...
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bioRxiv - Systems Biology 2020Quote: ... and 250 IU/mL IL-4 (Miltenyi Biotec) on days 1 ...
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bioRxiv - Immunology 2020Quote: ... interleukin 4 (IL4) (Miltenyi Biotec, Bergisch-Gladbach, Germany) was added to some wells at 10 ng/ml.
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bioRxiv - Immunology 2021Quote: ... truncated hCD4 gene (pMACS 4-IRES.II; Miltenyi Biotec) and 3’ loxP site flanked with arms of homology to exon 5 of the Il22 gene was recombineered into RP24-227B3 (see Figure S1A) ...
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bioRxiv - Cancer Biology 2023Quote: ... and 100ng/mL Human IL-4 (Miltenyi Biotec) for 5 days only adding media once in between ...
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bioRxiv - Cancer Biology 2023Quote: ... and 50ng/mL Human IL-4 (Miltenyi Biotec). Monocytes were plated into 6-well plates (5×105 cells ...
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bioRxiv - Immunology 2023Quote: ... Cells were depleted of CD4+ T cells by staining with CD4 PE-conjugated antibody (RM4-4) for 20 minutes at 4°C and then with Anti-PE MicroBeads (Miltenyi Biotec.) as described above ...
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bioRxiv - Immunology 2020Quote: ... cells were incubated at 37°C with 5% CO2 for 7 days and activated with M-CSF (1000 U/mL) (Miltenyi Biotec) every second day.
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bioRxiv - Cancer Biology 2023Quote: ... and an enzymatic cocktail (supplementary table 7) and digested at 37°C for 42 minutes (37C_m_TDK_2 program) on a gentleMACS Octo Dissociator (Miltenyi Biotec, 130-096-427). Cell suspensions were then filtered through a 40 μm cell strainer (Corning ...
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bioRxiv - Immunology 2023Quote: ... 1×106 vehicle or ACCi-conditioned OT-1 T cells were infused into B16-OVA tumor bearing mice 10 days after tumor challenge and tumors were harvested 7 days later using the Mouse Tumor Dissociation Kit and gentleMACS Octo dissociator with heaters (Miltenyi Biotec) according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2024Quote: ... for 2 days and transduced with EGFR LiTE- encoding lentiviruses (TSTAb) in the presence of 10 ng/ml interleukin (IL)-7 and 10 ng/ml IL-15 (Miltenyi Biotec). As negative control ...
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bioRxiv - Neuroscience 2021Quote: ... Nuclei pellets were washed tree times with one filtration step by MACS pre-separation filter 20 μm (Miltenyi Biotec). Nuclei pellets were resuspended in 100 μL of staining buffer (DPBS BSA 2% ...
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bioRxiv - Plant Biology 2019Quote: ... Supernatants were adjusted to 5 mg/mL protein concentration and incubated for 1 h at 4 °C with 100 μL of anti-GFP magnetic beads (Miltenyi Biotec, #130-091-125). Bound proteins were analyzed by immunoblotting with anti-GFP (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... using CD40L-multimere-kit and IL-4 (Miltenyi Biotec) according to manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: TILs were stimulated for 7 days with anti-biotin MACSiBead Particles loaded with biotinylated CD3/CD28 antibodies (Miltenyi Biotec, Bergisch Gladbach, Germany). The preparation of the conjugated beads was carried out according to the manufacture’s recommendation (activation/expansion kit ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were washed in 1%FBS in Dulbecco’s PBS 3 times before straining through 75 µm and 30 µm strainers (Greiner Bio-One and Miltenyi Biotec) in Dulbecco’s PBS ...
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bioRxiv - Cancer Biology 2020Quote: ... and subsequently stained at 4°C with antibodies against following human antigens: CD3 (REA613, APC-Vio770, Miltenyi Biotec, Cat no.130-113-698, 1:50), CD4 (A161A1 ...
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bioRxiv - Genomics 2024Quote: ... The Hofbauer/fibroblast fraction was then washed and incubated for 15 minutes at 4 °C in the dark with 1:5 anti-phycoerythrin MACS® MicroBeads (Miltenyi Biotec, #130-105-639). The Hofbauer/fibroblast fraction was loaded onto an MS column for magnetic separation ...
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bioRxiv - Molecular Biology 2021Quote: ... and 4 µL SOX17-PE (Miltenyi Biotec #130-111-032). We used FACS to sort populations into undifferentiated (FOXA2/SOX17 double negative ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 ng/ml IL-4 (#130-093-920, Miltenyi Biotec) for B cells ...
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bioRxiv - Neuroscience 2021Quote: ... Nuclei pellets were washed tree times in staining buffer with one filtration step by MACS pre-separation filter 20 μm (Miltenyi Biotec) to a final resuspension in 300 μl of staining buffer for Malassez cell counting with Trypan blue counterstaining (Trypan Blue solution ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 to 7 Nematostella polyps or 3 Aiptasia polyps constituted one biological sample and were transferred to tissue homogenization tubes (“C-tubes”, 130-096-334, Miltenyi Biotec) after they were relaxed ...
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bioRxiv - Neuroscience 2023Quote: ... Nuclei pellets were washed three times in staining buffer with one filtration step by MACS pre-separation filter 20 μm (Miltenyi Biotec) to a final resuspension in 300 μL of staining buffer for Malassez cell counting with Trypan blue counterstaining (Trypan Blue solution ...
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bioRxiv - Developmental Biology 2024Quote: ... The cells were pelleted and resuspended in FACS buffer for immunomagnetic cell separation with EpCAM-conjugated beads (Miltenyi Biotec, Catalog No. 130-105-958, 1/4). After 20 min incubation at 4°C ...
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bioRxiv - Neuroscience 2021Quote: Oligodendrocyte precursor cells (OPCs) were isolated from 5–7-day old YACQ84 transgenic mice using magnetic cell sorting (MACS, Miltenyi Biotec, Bergisch Gladbach, Germany). Whole brains were dissected and homogenized using the Neural Tissue Dissociation Kit P (Miltenyi ...
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bioRxiv - Genomics 2024Quote: ... Each fraction was washed with BSA rinsing buffer and incubated for 15 minutes at 4 °C in the dark with 1:5 anti-allophycocyanin MACS® MicroBeads (Miltenyi Biotec, #130-090-855, lot #5190809239). Each fraction was loaded onto an MS column (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... and PE-conjugated anti-IL-4 (clone 7A3-3, Miltenyi Biotec) following the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2021Quote: ... or 4) Oligodendrocytic (O4-APC (REA576, #130-119-982, Miltenyi Biotec)) fluorophore-conjugated antibodies ...
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bioRxiv - Immunology 2021Quote: ... and 20 ng/ml IL-4 (Miltenyi Biotec, Bergisch Gladbach, Germany) for M2-polarization ...
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bioRxiv - Immunology 2020Quote: ... at RT and permeabilized with InsidePerm at 4°C (Miltenyi Biotec) followed by incubation with anti-IL-10-PE-conjugated antibody (Miltenyi Biotec) ...
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bioRxiv - Molecular Biology 2023Quote: ... and 40 ng/ml IL-4 (#130-093-920, Miltenyi Biotec) for dendritic cells ...
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bioRxiv - Bioengineering 2022Quote: ... and then the cell pellet was resuspended in 100 µL of MACS buffer and 25 µL of anti-integrin α-7 microbeads (Miltenyi Biotec, #130-104-261) for incubation at 4 °C for 15 minutes ...
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bioRxiv - Immunology 2023Quote: ... Media was replenished every 48 hours for 7 days at which point BMDCs were harvested and magnetically selected using CD11c specific microbeads (Miltenyi Biotec, Cat# 130-125-835).