Labshake search
Citations for Miltenyi Biotec :
351 - 400 of 695 citations for 3 5 Diacetoxy 2 acetoxymethyl 6 phenethyl tetrahydro pyran 4 yl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... cut into 4 pieces and enzymatically digested by using the whole skin dissociation kit from Miltenyi biotec without enzyme P for 5 hours at 37°C under agitation (500 rpm) ...
-
bioRxiv - Immunology 2021Quote: ... Fc receptors were blocked for 10 minutes at 4°C with FcR blocking reagent (MACS Miltenyi Biotec). Cells harvested during the conversion period from CD34+ hematopoietic precursors to CD14+ monocytes (Days 12-21 of the differentiation protocol ...
-
bioRxiv - Immunology 2021Quote: ... in the presence of 50 ng/ml GM-CSF and 20 ng/ml IL-4 (Miltenyi Biotec). Untouched peripheral blood cDC1 and cDC2 (cDCs ...
-
bioRxiv - Cancer Biology 2020Quote: ... Cells were stained for 15min at 4°C and then transferred to an LS column (Miltenyi Biotec). Human cells were then cytospinned with Shandon cytofunnels (Thermo Fisher ...
-
bioRxiv - Immunology 2024Quote: ... PBMCs were incubated at 4 with magnetic microbeads conjugated to monoclonal anti-human CD14 Abs (Miltenyi Biotec) and isolated by positive magnetic selection according to manufacturer’s instructions (Miltenyi Biotec) ...
-
bioRxiv - Plant Biology 2024Quote: ... 4% BSA) and incubated in a primary antibody solution anti-GFP-HRP (1/5000, MACS Miltenyi Biotec) and 1/5000 anti-His (11667475001 ...
-
bioRxiv - Neuroscience 2023Quote: ... Cells were washed with HBSS and spun at 300 g for 5min at 4 °C (Miltenyi Biotec). The pellet was resuspended and incubated with 80 µL of MACS buffer and 20 µL of anti-IgM microbeads (Miltenyi Biotec ...
-
bioRxiv - Immunology 2024Quote: ... and stored at 4°C before being acquired using the MACSQuant Analyzer 10 flow cytometer (Miltenyi Biotec) and analyzed using FlowJo 10 ...
-
bioRxiv - Developmental Biology 2022Quote: Soft palatal tissue of E14.5 C57BL/6J mouse embryos was dissected and digested using Multi Tissue Dissociation Kit 3 (130-110-204, Miltenyi Biotec) at 37°C with shaking at 600 rpm for 15 minutes in a Thermomixer (2231000269 ...
-
bioRxiv - Developmental Biology 2022Quote: Soft palatal tissue of E14.5 C57BL/6J mouse embryos was dissected and digested using Multi Tissue Dissociation Kit 3 (130-110-204, Miltenyi Biotec) at 37°C with shaking at 600 rpm for 15 minutes in a Thermomixer (2231000269 ...
-
bioRxiv - Cancer Biology 2024Quote: ... tumors were harvested from mice on Day 3 and separately dissociated on a GentleMacs Octo Dissociator with Heaters (Miltenyi Biotec, Bergisch Gladbach ...
-
bioRxiv - Bioengineering 2024Quote: ... Thermo) were washed 3 times with PBS followed by incubation with biotin anti-mouse CD63 antibody (Clone REA563; Miltenyi Biotec) or biotin anti-mouse fibronectin (FN ...
-
bioRxiv - Developmental Biology 2024Quote: ... Cells were washed in 1%FBS in Dulbecco’s PBS 3 times before straining through 75 µm and 30 µm strainers (Greiner Bio-One and Miltenyi Biotec) in Dulbecco’s PBS ...
-
bioRxiv - Neuroscience 2023Quote: ... frozen hippocampus of 3 mice in each sample group were dissociated using the Adult Brain Dissociation kit (Miltenyi Biotec, Germany), and after dead cell removal ...
-
bioRxiv - Cancer Biology 2023Quote: ... and 3) removal of debris and dead cells using the Dead Cell Removal Kit (Miltenyi Biotec, Cat: 130-090-101). The brain of an age-matched uninoculated mouse was harvested and processed in parallel to serve as the negative control for downstream FACS sorting ...
-
bioRxiv - Molecular Biology 2024Quote: ... MDSL and TF-1 were additionally supplemented with 25ng/mL of recombinant human IL-3 (Miltenyi Biotec, 130-095-069), and MDS-L with 50nM β-mercaptoethanol (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2021Quote: ... microglia proliferation was induced by adding 5 ng/ml GM-CSF (Miltenyi Biotec, 130-095-746) to the complete medium ...
-
bioRxiv - Immunology 2021Quote: ... and IL-5 were enriched for live cells using a dead cell removal kit (Miltenyi Biotec) followed by CD138-APC (BD Pharmingen ...
-
bioRxiv - Neuroscience 2020Quote: ... microglia proliferation was induced by adding 5 ng/ml GM-CSF (Miltenyi Biotec, 130-095-746) to the complete medium ...
-
bioRxiv - Cancer Biology 2023Quote: ... We incubated with 5 µL of Annexin V-conjugated FITC (MACS Miltenyi Biotec, Lab Systems SA) at room temperature in darkness for 20 min ...
-
bioRxiv - Immunology 2024Quote: ... Some of the cultures were also supplemented with 5 ng/mL of exogenous cytokines (Miltenyi Biotec) recombinant human rh-IL15 or rh-IL15+rh-IL21 ...
-
bioRxiv - Genomics 2024Quote: ... we combined 300 mg of the 5 SAT biopsies into a gentleMACS C tube (Miltenyi Biotec) containing 3 ml of chilled 0.1X lysis ...
-
bioRxiv - Microbiology 2020Quote: ... in complete RPMI-1640 supplemented with 80 U/ml IL-2-IS (Miltenyi Biotec) and cultured in 96-well V-bottom plates ...
-
bioRxiv - Cell Biology 2020Quote: ... Following astrocyte staining with ACSA-2-APC (Clone IH3-18A3, 1:200, Miltenyi Biotec), Hoechst to discriminate between cells and debris (10 µg/ml ...
-
bioRxiv - Immunology 2020Quote: NK cells were expanded by culturing with IFN-α and IL-2 (Miltenyi Biotec) in cIMDM for 48 hours to induce priming ...
-
bioRxiv - Cell Biology 2020Quote: ... and in presence of 33 U/ml human IL-2 (premium-grade, Miltenyi Biotec).
-
bioRxiv - Immunology 2020Quote: ... and 50 U/ml of human IL-2 IS (Miltenyi Biotec, #130-097-142) for primary T cells ...
-
bioRxiv - Immunology 2021Quote: ... then positively selected using anti-ACSA-2 MicroBeads (Miltenyi Biotec, Cat# 130-097-678). Total RNA was prepared using TRI Reagent (Millipore-Sigma ...
-
bioRxiv - Neuroscience 2022Quote: ... astrocyte ACSA-2+ cell sorting was performed using an autoMACS Pro Separator (Miltenyi Biotec). After isolation ...
-
bioRxiv - Microbiology 2024Quote: Splenocytes were incubated with SARS-CoV-2 S peptide pools (1μg/ml, Miltenyi Biotec) for 6 h in the presence of GolgiPlug (BD Bioscience) ...
-
bioRxiv - Developmental Biology 2024Quote: ... 2-photon imaging was also performed with a TrimScope Matrix II from Miltenyi Biotec using an Olympus 25X W/NA 1.05 objective at a wavelength of 850 nm owing to Spectra Physics Insight X3+ laser.
-
bioRxiv - Bioengineering 2024Quote: ... at a ratio of 1:2 in NK MACS media (Miltenyi Biotec, Gaithersburg, MD) supplemented with 5% human AB serum (Millipore Sigma ...
-
bioRxiv - Neuroscience 2023Quote: ... followed by ACSA-2 MicroBeads (cat # 130-097-678, MACS Miltenyi Biotec, Auburn, CA) for 15 min ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were stimulated with IL-2 (10 ng/ml) (Miltenyi Biotec #130-097-745) and with anti-CD3/CD28 beads (Gibco #11132D ...
-
bioRxiv - Genetics 2024Quote: ... iPSCs were mechanically passaged in the presence of 2 μM Thiazovivin (Miltenyi Biotec, UK).
-
bioRxiv - Immunology 2024Quote: ... SARS-CoV-2 Prot_S 130127953) (0.6 nmol/mL each) (Miltenyi Biotec; Bergisch Gladbach, Germany). After 6 days of incubation at 37°C ...
-
bioRxiv - Developmental Biology 2024Quote: ... Cells were then incubated with anti-APC magnetic beads (Miltenyi Biotec, Catalog No. 130-090-855, 1/4) for 20 min at 4°C ...
-
bioRxiv - Cell Biology 2020Quote: Pre-conditioned media from FAP cell culture stimulated 3 days with PDGF-AA (20 ng/ml, human PDGF-AA, Cat nb. 130-108-983, Miltenyi Biotec) or control was collected and stored at −20°C ...
-
bioRxiv - Developmental Biology 2020Quote: ... Cell clumps and debris were removed by two consecutive rounds of centrifugation 3 minutes at 200xg and filtration through 30 µm cell strainers (Miltenyi Biotec). Additional mechanical dissociation and removal of cell aggregates was achieved by gentle pipetting up and down 5 times using a P200 pipette and Gel Saver tips (QSP) ...
-
bioRxiv - Immunology 2020Quote: ... after which 3 mL of FACS buffer was added and the mixture was passed over a magnetized LS column (Miltenyi Biotec). The column was washed once with 5 mL ice-cold FACS buffer and then removed from the magnetic field and 5 mL ice-cold FACS buffer was pushed through the unmagnetized column twice using a plunger to elute the bound cell fraction.
-
bioRxiv - Immunology 2021Quote: ... 21 and 63 DPI samples (n=3) were depleted of alveolar macrophages using a MACS cell separation LS column (Miltenyi Biotec) after staining for CD14 and CD172a (see Table S2) ...
-
bioRxiv - Genomics 2021Quote: ... cells were blocked with 3%BSA diluted in PBS for 1h at RT and then incubated with anti-CD-19-PE antibody (1:50) in 3% BSA-PBS (Miltenyi Biotec) for 1h at RT ...
-
bioRxiv - Developmental Biology 2022Quote: The soft palate from E13.5 embryos was dissected and digested using Multi Tissue Dissociate Kit 3 (Miltenyi Biotec, 130-110-204) at 37°C (600 rpm for 15 minutes ...
-
bioRxiv - Developmental Biology 2022Quote: ... Remaining clumps of cells and debris were removed via two rounds of centrifugation for 3 min at 200 g followed by filtration through 30 μm cell strainers (Miltenyi Biotec). To ensure all remaining cells aggregates were dissociated ...
-
bioRxiv - Biophysics 2022Quote: ... Naive T cells were purified via magnetic negative selection from spleens of 6–8 week old mice using either CD4+ (for 3.L2) or CD8+ (for P14 and OT1) T-cell isolation kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2023Quote: ... 1 to 7 Nematostella polyps or 3 Aiptasia polyps constituted one biological sample and were transferred to tissue homogenization tubes (“C-tubes”, 130-096-334, Miltenyi Biotec) after they were relaxed ...
-
bioRxiv - Cancer Biology 2022Quote: ... standard CAR-T cells for the clinical setting were produced except for several modifications(20): CD8+ T cells were stimulated with 1 ug/ml OKT-3/CD28 antibodies (Miltenyi Biotec) for 72 h ...
-
bioRxiv - Cell Biology 2024Quote: ... hFlt3-Ligand 100ng/mL, 130-096-479; hIL-3 60ng/mL, 130-095-069; TPO 10nM, 130-094-013, all from Miltenyi Biotec) and 1% PS during 24 hours ...
-
bioRxiv - Molecular Biology 2021Quote: ... we stained batches of 5 million cells with 20 µL FOXA2-APC (Miltenyi Biotec #130-107-774) and 4 µL SOX17-PE (Miltenyi Biotec #130-111-032) ...
-
bioRxiv - Genomics 2022Quote: ... cells were dissociated using TrypLE for 5 min and sorted using CD144-conjugated magnetic microbeads (Miltenyi Biotec) according to the manufacturer’s instructions ...