Labshake search
Citations for Miltenyi Biotec :
351 - 400 of 430 citations for 2 Triisopropylsilyl Oxazole 5 Carbaldehyde since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Synthetic Biology 2023Quote: ... supplemented with 10% Human AB serum (Gemini Bio Products) with the addition of 100 U/ml human IL-2 IS (Miltenyi Biotec), 1 ng/ml IL-15 (Miltenyi Biotec) ...
-
bioRxiv - Immunology 2024Quote: ... In-vitro CD4 T cell survival assays were performed in complete RPMI medium supplemented with recombinant murine IL-2 (100 ng/ml, Miltenyi Biotec) or IL-7 (10 ng/ml ...
-
bioRxiv - Immunology 2024Quote: ... Pieces of approximately 1 cm2 were placed in 10 mL PBS containing 2% FBS and homogenized for 10min at room temperature using a gentleMACS dissociator (Miltenyi Biotec). Homogenized tissue was filtered through a 70 μm cell strainer and pooled ...
-
bioRxiv - Cell Biology 2024Quote: ... cells were stained with ITGA7-APC and ITGA5-PE (Supplementary Table 2) and sorted using a MACSQuant Tyto Cell Sorter (Miltenyi Biotec). Flow cytometry was performed regularly during SC proliferation using a MACSQuant10 Flow Analyzer (Miltenyi Biotec ...
-
bioRxiv - Immunology 2024Quote: ... stimulated with IL-2 (R&D), and/or T cell differentiating cytokines and antibodies (Th1: anti-IL-4, IL-12 (Miltenyi Biotec), Treg ...
-
bioRxiv - Neuroscience 2024Quote: ... Then the astrocytes or newborn neurons were isolated using Anti-ACSA-2 MicroBead Kit or Anti-PSA- NCAM MicroBead Kit (Miltenyi Biotec). Briefly to say ...
-
bioRxiv - Immunology 2024Quote: ... Cells were resuspended in 900 µl/107 of isolation buffer containing 0.5% Bovine Serum Albumin (BSA) and 2 mM EDTA in PBS and 10 µl/1x107 of mouse anti- Ly6G MicroBeads (Miltenyi Biotec) and incubated at 4°C for 10 mins ...
-
bioRxiv - Cell Biology 2024Quote: ... 20 ng ml−1 mouse recombinant vascular endothelial growth factor and 20 ng ml−1 human recombinant fibroblast growth factor-2 (all from Miltenyi Biotec) as described 85 and plated on pre-coated (Collagen I (10 µg ml−1 Millipore) ...
-
bioRxiv - Cancer Biology 2024Quote: CD34+ cells were enriched from BM MNCs of sex-matched SF3B1-mutated MDS patients (n = 3) and healthy donors (n = 2) using CD34 Microbeads and positive selection with autoMACS Pro Separator (Miltenyi Biotec). 4000 or 7500 cells were plated as duplicates in MethoCult (H4434 ...
-
bioRxiv - Immunology 2021Quote: ... Cells were centrifuged at 400 g for 5 min and incubated with 1:25 diluted mouse FcR blocking reagent (Miltenyi Biotec, 130-092-575) in FACS Buffer (PBS ...
-
bioRxiv - Immunology 2021Quote: CD4+ T cells were isolated from at least 5×106 PBMCs on the day of thaw by negative selection using the CD4+ T cell isolation kit II (Miltenyi Biotec, Bergisch Gladbach, Germany) according to manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: Oligodendrocyte precursor cells (OPCs) were isolated from 5–7-day old YACQ84 transgenic mice using magnetic cell sorting (MACS, Miltenyi Biotec, Bergisch Gladbach, Germany). Whole brains were dissected and homogenized using the Neural Tissue Dissociation Kit P (Miltenyi ...
-
bioRxiv - Neuroscience 2021Quote: ... a 5 µl volume of phosphate-buffered PBS (PBS) containing 10 μg/kg/injection of recombinant mouse IL-1β (Miltenyi Biotec, Bergisch Gladbach, Germany) or of PBS alone (control ...
-
bioRxiv - Genetics 2022Quote: ... in a cell culture incubator (37 °C; 5% CO2) followed by dissociation in gentleMACS C Tubes (Miltenyi Biotec Inc., cat. no. # 130-093-237) in a total of 8 ml DMEM media with 10% FBS using gentleMACS Dissociator Program A twice and seeded onto 15 cm tissue culture dishes and grown in DMEM with 10% FBS at 37 °C with 5% CO2.
-
bioRxiv - Immunology 2022Quote: ... After centrifugation (5 min, 500 x g, 4 °C) supernatant was discarded and cells were resuspended in MACS buffer (Miltenyi Biotec, #130-091-221) for following cell sorting.
-
bioRxiv - Cell Biology 2023Quote: ... The tissue pieces were transferred to a 5 ml conical tube containing Mix 1 from the Neural Tissue Dissociation Kit (Miltenyi Biotec, 130-092-628) and incubated on a shaker for 15 minutes at room temperature ...
-
bioRxiv - Cancer Biology 2023Quote: ... the sample is spun down at 400 g for 5 min at 4℃ and subject to the dead cell removal kit (Miltenyi Biotec, 130-090-101). Finally the sample is spun down at 400 g for 5 min at 4℃ and resuspended in 500 μL to 1 mL of PBS with 0.5% BSA to a final concentration of 700 to 1,500 cells per μl.
-
bioRxiv - Developmental Biology 2024Quote: ... dissociated cells were centrifuged and resuspended in a 5% fetal bovine serum in PBS (FBS/PBS) with CD133-PE (Miltenyi Biotec 130-080-801), CD44-FITC (BD Pharmingen 555478 ...
-
bioRxiv - Neuroscience 2024Quote: E15.5 embryo brains were extracted and cortices were dissociated into single-cell suspension using Neural Tissue Dissociation Kit (P) (Miltenyi Biotec, 130-092-628). Progenitors were magnetically selected and isolated using Anti-Prominin1 MicroBeads (Miltenyi Biotec ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Dynabead-EV samples were re-suspended into 98 μl DPBS and 2 μl of one of the following antibodies: IgG1-PE (#130-113-200, Miltenyi Biotec, Germany), CD9-PE ...
-
bioRxiv - Cancer Biology 2021Quote: Surgical specimens placed immediately in cold sterile DMEM after excision were cut into 2-4 mm pieces before undergoing enzymatic and mechanical dissociation using the gentleMACS™ Octo Dissociator multitissue kit and protocol (Miltenyi Biotec). Resuspended cells were then promptly plated into flasks ...
-
bioRxiv - Bioengineering 2021Quote: ... Nalm6 tumors were extracted on day 4 post bacteria treatment (day 2 post T cell treatment) and lymphocytes were isolated from tumor tissue by mechanical homogenization using a gentleMACS™ Dissociator (Miltenyi Biotec) in complete hTcm ...
-
bioRxiv - Microbiology 2022Quote: ... 1 ml of each sample was transferred to a 2 ml Eppendorf tube and the samples were assayed on a flow cytometer (MACSQuant® VYB, Miltenyi Biotec). Green fluorescent cells were detected using the blue laser (488 nm ...
-
bioRxiv - Microbiology 2022Quote: ... and stimulated with either SARS-CoV-2 S-trimer (10-100 µg/mL) or SARS-CoV-2 PepTivator® Peptide S and NP protein Pools (10 µg/mL each, Miltenyi Biotec) for 72 h at 37°C ...
-
bioRxiv - Developmental Biology 2022Quote: ... The remaining cells were pelleted and resuspended in 80 µl of MACS buffer (PBS pH 7.2, 0.5% BSA and 2 mM EDTA) and 20 µl anti-CD45 MicroBeads (Miltenyi Biotec #130-052-301) or 20 µl anti-F4/80 MicroBeads (Miltenyi Biotec #130-110-443 ...
-
bioRxiv - Biochemistry 2021Quote: ... The cell pellet was resuspended in 80 μL of 2% BSA/PBS and incubated with 20 μL of anti-PE MicroBeads UltraPure (Miltenyi Biotec, USA.) for 15 min at 4°C ...
-
bioRxiv - Neuroscience 2021Quote: ... 2 mM EDTA in PBS) and labeled with anti-mouse CD31-PE (1:50, #130-111-354, Miltenyi Biotec, Auburn, CA, USA), anti-mouse CD45-FITC (1:50 ...
-
bioRxiv - Microbiology 2020Quote: Tissue samples were homogenized 2 × 55 sec in 1 ml sterile phosphate buffer saline (PBS) using a gentleMACS™ Octo Dissociator (Miltenyi Biotec). Total DNA from bacterial cells and infected tissues was purified using a GeneJet Genomic DNA Purification Kit (Thermo Scientific ...
-
bioRxiv - Immunology 2021Quote: ... 2 mM EDTA in PBS) before positive selection of CD14+ monocytes by incubation with magnetic CD14 microbeads (Miltenyi Biotec, 130-050-201) (15 min ...
-
bioRxiv - Cancer Biology 2023Quote: ... Tumors were cut in small pieces of approximately 2 mm3 then incubated with Human tumor dissociation kit (Miltenyi Biotec, #130-095-929) and placed at 37°C in agitation (500 rpm ...
-
bioRxiv - Cell Biology 2023Quote: ... cut longitudinally into roughly 2-mm-long pieces and were isolated using the Liver Dissociation Kit (mouse) and GentleMACS Dissociator (Miltenyi Biotec, Germany) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... + 0.5x GlutaMax (Thermo 35050061) + 1x NEAA (Thermo 11140050) + 1x N-2 (Thermo 17502048) + 1x MACS NeuroBrew-21 (Miltenyi Biotec 130-093-566). On D3 ...
-
bioRxiv - Cell Biology 2023Quote: ... tissues were cleansed in ice-cold RPMI1640 and dissociated using the Multi-tissue dissociation kit 2 (Catalog No.130-110-203, Miltenyi Biotec, USA) following the manufacturer instructions ...
-
bioRxiv - Immunology 2024Quote: CD4+ and CD8+ T cells and CD14+ monocytes were isolated from healthy donor mononuclear cells (agreement ANG-2003-2) by positive magnetic sorting (Miltenyi Biotec, Germany). Macrophages were generated by culturing monocytes for 7 days with 50 ng/ml M-CSF or 50 ng/ml GM-CSF (ORF Genetics Kopavogur ...
-
bioRxiv - Immunology 2024Quote: ... both CD45.2 DKO and WT polyclonal donor marrow was depleted of mature T cells by magnetic column-based negative selection (Miltenyi Biotec MACS) using an anti-mouse CD5 biotin-conjugated antibody (Clone 53-7.3 ...
-
bioRxiv - Cell Biology 2020Quote: ... LT-HSCs were enriched by positive selection for c-Kit expression using anti-CD117 immunomagnetic beads (130-091-224, Miltenyi Biotec Inc. 1:5 dilution). Second ...
-
bioRxiv - Immunology 2020Quote: ... BM cells were incubated on ice for 20 minutes with c-Kit microbeads (Miltenyi Biotec, 130-091-224; 5 μl/100 μl SM per mouse), washed with SM and enriched on an AutoMACS Pro magnetic cell separator (Miltenyi Biotec) ...
-
bioRxiv - Developmental Biology 2022Quote: ... and cells were resuspended in 5 mL MACS Buffer (autoMACS Rinsing Solution [Miltenyi Biotec, 130-091-222] with MACS BSA Stock Solution [Miltenyi Biotec, 130-091-376]) and passed through a 40 μm filter (Greiner Bio-One ...
-
bioRxiv - Immunology 2023Quote: ... Each lung was then fragmented using surgical scissors and transferred to 5 mL of dissociation media (1 lung per dissociation reaction) in gentleMACS™ C Tubes (Miltenyi Biotec, 130-093-237). Dissociation media contained 0.5 U/mL dispase (Stemcell Technologies ...
-
bioRxiv - Genomics 2024Quote: ... The Hofbauer/fibroblast fraction was then washed and incubated for 15 minutes at 4 °C in the dark with 1:5 anti-phycoerythrin MACS® MicroBeads (Miltenyi Biotec, #130-105-639). The Hofbauer/fibroblast fraction was loaded onto an MS column for magnetic separation ...
-
bioRxiv - Genomics 2024Quote: ... an aliquot of cells per sample was centrifuged at 500 x g for 5 min at RT and used for dead cell removal (Miltenyi Biotec, ref. 130-090-101) using the autoMACS® Pro Separator following manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... 2 mg of each cell lysate was incubated with 40 μL Anti-HA Microbeads (μMACS HA Isolation Kit, Miltenyi Biotec, 130-091-122) in a volume of 500 μL for 45 min at 4 °C with rotation ...
-
bioRxiv - Developmental Biology 2020Quote: ... cell suspension with PBS supplemented with 0.5% BSA and 2 mM EDTA was applied to an MS column (Miltenyi Biotec, 130-042-201). After negative cells flow through the column ...
-
bioRxiv - Microbiology 2023Quote: Approximately half of the lung and the left hemisphere of each brain were weighed and homogenized in 1 mL of DMEM supplemented with 2% FBS in GentleMACS™ M tube using GentleMACS™ Dissociator (Miltenyi Biotec) with RNA 2.01 program setting ...
-
bioRxiv - Neuroscience 2022Quote: ... pellet was resuspended in 100 μl of PBS containing the appropriate antibodies: anti-ACSA-2-APC (1:50, Miltenyi Biotec, 130-117-535) and anti-GLUT1 conjugated to Alexa Fluor 488TM (1:200 ...
-
bioRxiv - Developmental Biology 2024Quote: ... The cortex was cut in 2-3 mm pieces and dissociated in Neural Tissue Dissociation Kit P compounds (Miltenyi Biotec, 130-092-628) to obtain a cell suspension ...
-
bioRxiv - Microbiology 2024Quote: ... The samples were investigated at RT with 2-Photon imaging using two laser lines in parallel and PBS as the optical medium (LaVision Bio Tec TrimpScope 2; Miltenyi Biotec, Bielefeld, Germany). mCherry was excited with a Spectra-Physics Insight laser set to 1105 nm and GFP and dermal collagen were visualized by a second laser set to 910 nm at an intensity of 1 to 10% of its maximum power ...
-
bioRxiv - Neuroscience 2024Quote: ... + 0.5% bovine serum albumin (BSA) + 2 mM EDTA + 0.05% sodium azide) and incubated with 25 μL CD11b (Microglia) microbeads (Miltenyi Biotec, #130-093-634) on ice for 30 min ...
-
bioRxiv - Neuroscience 2020Quote: Isolated mononuclear cells from five brains were then further purified by CD11b positive selection using MACS columns (N = 5 mice, >90% enrichment of CD11b+ microglia [25]) (Miltenyi Biotec, Cat. No. 130–093-636) to selectively enrich microglia and brain mononuclear phagocytes (Figure 1B) ...
-
bioRxiv - Immunology 2020Quote: ... The enriched B cells were resuspended in 1 % BSA in PBS x1 and 2 mM EDTA and stained with anti-CD19-FITC (Miltenyi Biotec, 130-113-645), anti-igG-APC (Miltenyi Biotec ...