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Citations for Miltenyi Biotec :
351 - 400 of 1064 citations for 2 5 Sulfanyl 4H 1 2 4 triazol 3 yl phenol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... 25 ng/ml IL-3 (Miltenyi Biotec), 2 mM Glutamax (Invitrogen Life Technologies) ...
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bioRxiv - Neuroscience 2024Quote: ... 25 ng/ml IL-3 (Miltenyi Biotec), 100 ng/ml M-CSF (Miltenyi Biotec) ...
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bioRxiv - Immunology 2020Quote: ... The cell suspensions were incubated with running buffer (PBS supplemented with 0.5% FCS and 2 mM EDTA) and L3T4 microbeads (Miltenyi Biotec, Maquarie Park, NSW, Australia) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... mixture at room temperature for 30 minutes and subsequently adding into 10 mL of RPMI+10%FBS and 2 uL of IL4 (Miltenyi Biotec 130-106-196). Cells were seeded at 0.5 × 106 per mL of CD40 stimulation medium at 37 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... frozen single cell suspensions from MDOTS processing (see above) were thawed and CD8+ TILs were isolated using Cd8a (LY-2) Microbeads (Miltenyi Biotec, 130-117-044). T cells were cultured in RPMI (Corning ...
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bioRxiv - Immunology 2023Quote: ... the cells were washed with PBS and resuspended in 50 µl of 1X annexin binding buffer containing 2 µl of FITC-annexin V (Miltenyi Biotec, 130-092-052). Cells were then incubated at room temperature for 30 min in the dark ...
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bioRxiv - Cancer Biology 2022Quote: ... samples were cut into approximately 2 – 4mm chunks with forceps and a scalpel and transferred to a gentleMACS C tube (Miltenyi Biotec, 130-093-237) containing 4.7 ml RPMI 1640 base media ...
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bioRxiv - Immunology 2022Quote: ... γδT cells were washed three times before co-culturing at a ratio of 1:5 with CD8+T cells isolated from tumor-bearing mice using CD8a (Ly-2) microbeads through magnetic activated cell sorting (MACS) (Miltenyi Biotec, Bergisch Gladbach, Germany). Tumor cell lysate (TCL ...
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bioRxiv - Neuroscience 2024Quote: ... The tissue was then minced and transferred into a 2 ml Dounce homogenizer containing nuclei extraction buffer (130-128-024, Miltenyi Biotec, Bergisch Gladbach, Germany) along with 0.2 U/ml Ribolock RNase inhibitor ...
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bioRxiv - Genomics 2024Quote: ... THP-1 in vitro differentiated macrophages were then stimulated by replacing media with 2 mL of fresh media containing 100 ng/mL of IFNγ (Miltenyi Biotec, 130-096-484) for 4 hours ...
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bioRxiv - Genomics 2024Quote: ... 4°C) with the surface antibody CD34-PE-Vio770 (clone AC136, lot no. 5180718070, dilution 1:50, Miltenyi Biotec) and DAPI (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then washed and resuspended in MACS buffer plus 1:5 anti-mouse igG microbeads (Miltenyi Biotec) and rotated at 4°C for a further 15 min ...
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bioRxiv - Genomics 2021Quote: ... cells were blocked with 3%BSA diluted in PBS for 1h at RT and then incubated with anti-CD-19-PE antibody (1:50) in 3% BSA-PBS (Miltenyi Biotec) for 1h at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... standard CAR-T cells for the clinical setting were produced except for several modifications(20): CD8+ T cells were stimulated with 1 ug/ml OKT-3/CD28 antibodies (Miltenyi Biotec) for 72 h ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... Cells were centrifuged and resuspended with fresh media with 40 μL/mL of PeptTivator® SARS-CoV-2 Prot_S (Miltenyi Biotec, Cat# 130-126-700), 1 μL/mL monensin (BioLegend) ...
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bioRxiv - Immunology 2021Quote: ... and the respective SARS-CoV-2 peptide pool (0.6 nmol/peptide, S and N pools from Miltenyi Biotec, 130-126-701 and 130-126-699). Unstimulated samples were used for subtraction of background cytokine production ...
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bioRxiv - Immunology 2020Quote: Pools of lyophilized peptides covering the immunodominant sequence of the spike glycoprotein and the complete sequence of the membrane glycoprotein of SARS-CoV-2 (15-mer sequences with 11 amino acids overlap) were obtained from Miltenyi Biotec (Thieme et al., 2020) resuspended and stored according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2023Quote: ... The lungs were incubated for 20min at 37 ◦C under continuous rotation and then run on the gentleMACS m-Lung-2 program on the gentleMACS (Miltenyi Biotec, Cat #130-095-937). This was followed by brief centrifugation and the passage of cells through the 70-uM filter ...
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bioRxiv - Immunology 2024Quote: ... AVX/COVID-12 or 30 nM of a mixture of 10-15 amino acid long peptides derived from the SARS-CoV-2 spike protein (PepTivator, Miltenyi Biotec, North Rhine-Westphalia, Germany). In parallel ...
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bioRxiv - Developmental Biology 2022Quote: ... The lungs were incubated for 20min at 37 ◦C under continuous rotation and then run on the gentleMACS m-Lung-2 program on the gentleMACS (Miltenyi Biotec, Cat #130-095-937). This was followed by brief centrifugation and the passage of cells through the 70-uM filter ...
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bioRxiv - Synthetic Biology 2024Quote: ... the beads were removed and IL-2 was replaced with IL-7 and IL-15 (Miltenyi Biotec GmbH, 130-095-367 and 130-096-567) at 10 ng/ml each ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-OCT3/4 (PE; Miltenyi Biotec), and anti-NANOG (BV421 ...
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bioRxiv - Immunology 2021Quote: ... IL-4 Catch Reagent (Miltenyi Biotec) in a final volume of 100 µL per 106 cells for 15 min on ice ...
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bioRxiv - Neuroscience 2023Quote: ... and cells were washed and labeled with the following antibodies for 30 min at 4 °C: rat anti-CD45 PerCP/Cyanine5.5 (1:100, catalog no. 130-102-469; Miltenyi Biotec); rat anti-SiglecH PE (1:100 ...
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bioRxiv - Immunology 2024Quote: ... conjugate and staining information are as follows: anti-human CD3 (Miltenyi Biotec, Clone BW264/56, APC-R700, 1:200, 4℃); anti-human CD19 (BioLegend ...
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bioRxiv - Cancer Biology 2024Quote: ... the slides were further incubated overnight at 4°C with anti-PD1 antibody (Miltenyi Biotec, 130-117-384; 1:100) and anti-CK antibody (Invitrogen ...
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bioRxiv - Genomics 2020Quote: ... and passed through 3 LS Columns (Miltenyi Biotec). Mouse embryonic stem cells (ESC ...
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bioRxiv - Immunology 2020Quote: ... IL-5-Vio515 (Miltenyi Biotec), IL-4-PE Dazzle594 and IL-13-BV421 (Biolegend) ...
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bioRxiv - Immunology 2020Quote: ... in combination with or without recombinant interleukin-4 (IL-4, Miltenyi Biotec, catalogue number: 130-094-061) for 24h ...
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bioRxiv - Neuroscience 2020Quote: ... Resuspended total EVs were incubated with 4 μg of anti-GLAST (i.e. antibody against the astrocyte cell surface antigen-1; RRID: AB_2733473; Miltenyi Biotec, Auburn, CA), anti-human CD171 (i.e ...
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bioRxiv - Cancer Biology 2022Quote: Cryopreserved grafts were thawed and stained using standard procedures (10 min, 4°C) with the surface antibody CD34-PE-Vio770 (clone AC136, lot# 5180718070, dilution 1:50, Miltenyi Biotec) and DAPI (Sigma-Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... spleens were harvested and single cell suspensions were incubated with APC-conjugated I-Ab Mtb Ag85b280-294 tetramer (provided by National Institutes of Health tetramer core facility) for 1 hour at 4°C and tetramer-positive cells were enriched using anti-APC Micro-Beads (Miltenyi Biotec). CD19−CD11c−CD11b−F4/80−CD8a− WT (CD45.1+CD45.2+ ...
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bioRxiv - Neuroscience 2024Quote: ... for 10 min at 4°C and incubated with anti-mouse CD11b-PE (1:50 dilution; 130-113-806, Miltenyi Biotec) and anti-mouse CD45-APC (1.15:100 dilution ...
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bioRxiv - Cell Biology 2024Quote: ... and resuspended in cold selection buffer (PBS + 0.5% BSA + 2 mM EDTA) mixed with 20% (v/v) CD20 MicroBeads (Miltenyi Biotec #130-091-104 and #130-042-201) for 15 min at 4 ºC ...
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bioRxiv - Immunology 2020Quote: ... Th2: 20ng/ml rmIL-4 (Miltenyi Biotec). Intracellular cytokine staining was performed after incubation for 4-6h with Cell Stimulation cocktail plus Golgi transport inhibitors (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... 10 ng/mL BMP-4 (Miltenyi Biotec), and 10 µM Y-27632 (Abcam ...
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bioRxiv - Immunology 2024Quote: ... BDCA-4-magnetic beads (MACS Miltenyi Biotec), LS Columns (MACS Miltenyi Biotec ...
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bioRxiv - Molecular Biology 2024Quote: ... + 30 ng/mL BMP-4 (Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Immunology 2020Quote: ... Lineage cells (Ter119+ F4/80+ Gr1+) were depleted using magnetic anti-biotin Microbeads (1:5) (Miltenyi Biotec 130-090-485) and MS columns (Miltenyi Biotec 130-042-201) ...
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bioRxiv - Developmental Biology 2022Quote: ... using the Multi Tissue Dissociation Kit 3 (Miltenyi Biotec), and pooled ...
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bioRxiv - Cell Biology 2024Quote: ... 3 μM of StemMACS™ CHIR99021 (CHIR; Miltenyi Biotec) were also added ...
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bioRxiv - Neuroscience 2024Quote: ... The cell pellet was then resuspended in 100 µl of cold D-PBS containing fluorochrome-conjugated antibodies recognizing mouse CD11b and ACSA-2 (Miltenyi Biotec, 130-113-810 and 130-116-247) in a 1:100 dilution and incubated for 10 min at 4°C in the dark ...
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bioRxiv - Immunology 2020Quote: ... plated in 6-well culture plates at a concentration of 5 × 106 cells/ml in 1 ml of serum-free TexMACS medium (Miltenyi Biotec) and left overnight (5 % CO2 ...
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bioRxiv - Molecular Biology 2023Quote: ... following which the cells were cryopreserved at a concentration of 5×106-1×107 cells/mL in CryoMACS 50 or 250 Bags (Miltenyi Biotec) for the gene edited drug product generation step.
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bioRxiv - Systems Biology 2020Quote: ... and 250 IU/mL IL-4 (Miltenyi Biotec) on days 1 ...
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bioRxiv - Immunology 2020Quote: ... interleukin 4 (IL4) (Miltenyi Biotec, Bergisch-Gladbach, Germany) was added to some wells at 10 ng/ml.
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bioRxiv - Immunology 2021Quote: ... truncated hCD4 gene (pMACS 4-IRES.II; Miltenyi Biotec) and 3’ loxP site flanked with arms of homology to exon 5 of the Il22 gene was recombineered into RP24-227B3 (see Figure S1A) ...
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bioRxiv - Cancer Biology 2023Quote: ... and 100ng/mL Human IL-4 (Miltenyi Biotec) for 5 days only adding media once in between ...
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bioRxiv - Cancer Biology 2023Quote: ... and 50ng/mL Human IL-4 (Miltenyi Biotec). Monocytes were plated into 6-well plates (5×105 cells ...
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bioRxiv - Cancer Biology 2022Quote: ... 30 ng/ml anti-CD3 antibody (OKT-3) (Miltenyi Biotec) and 3000 IU/ml hrIL-2 ...