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Citations for Miltenyi Biotec :
351 - 400 of 474 citations for 2 4 Benzyl 2 2 dimethyl tetrahydro pyran 4 yl ethylamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... The dissociated cells were enriched for epithelial cells by Magnetic-activated cell sorting (MACS) (buffer: 1x PBS, 1% BSA, and 2 mM EDTA) with CD326 (EpCAM) microbeads (Miltenyi Biotec, 130-061-101) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Spleen cells were incubated at 4°C with anti-CD4 (L3T4)-MicroBeads (Miltenyi Biotec) after 10 min CD4 (RM4-5)-PB ...
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bioRxiv - Developmental Biology 2023Quote: ... for 20 min at 4°C and passed through a LS column (Miltenyi Biotec). The flow-through was collected and used for cell surface staining with the antibodies listed in Table S1 ...
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bioRxiv - Developmental Biology 2023Quote: ... for 20 min at 4 °C and passed through a LS column (Miltenyi Biotec). The flow-through was collected and used for cell surface staining ...
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bioRxiv - Developmental Biology 2023Quote: ... for 20 min at 4°C and passed through a LS column (Miltenyi Biotec). The flow-through was collected and used for cell surface staining ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... Cells were centrifuged and resuspended with fresh media with 40 μL/mL of PeptTivator® SARS-CoV-2 Prot_S (Miltenyi Biotec, Cat# 130-126-700), 1 μL/mL monensin (BioLegend) ...
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bioRxiv - Immunology 2021Quote: ... and the respective SARS-CoV-2 peptide pool (0.6 nmol/peptide, S and N pools from Miltenyi Biotec, 130-126-701 and 130-126-699). Unstimulated samples were used for subtraction of background cytokine production ...
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bioRxiv - Immunology 2020Quote: Pools of lyophilized peptides covering the immunodominant sequence of the spike glycoprotein and the complete sequence of the membrane glycoprotein of SARS-CoV-2 (15-mer sequences with 11 amino acids overlap) were obtained from Miltenyi Biotec (Thieme et al., 2020) resuspended and stored according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2023Quote: ... The lungs were incubated for 20min at 37 ◦C under continuous rotation and then run on the gentleMACS m-Lung-2 program on the gentleMACS (Miltenyi Biotec, Cat #130-095-937). This was followed by brief centrifugation and the passage of cells through the 70-uM filter ...
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bioRxiv - Immunology 2024Quote: ... AVX/COVID-12 or 30 nM of a mixture of 10-15 amino acid long peptides derived from the SARS-CoV-2 spike protein (PepTivator, Miltenyi Biotec, North Rhine-Westphalia, Germany). In parallel ...
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bioRxiv - Developmental Biology 2022Quote: ... The lungs were incubated for 20min at 37 ◦C under continuous rotation and then run on the gentleMACS m-Lung-2 program on the gentleMACS (Miltenyi Biotec, Cat #130-095-937). This was followed by brief centrifugation and the passage of cells through the 70-uM filter ...
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bioRxiv - Synthetic Biology 2024Quote: ... the beads were removed and IL-2 was replaced with IL-7 and IL-15 (Miltenyi Biotec GmbH, 130-095-367 and 130-096-567) at 10 ng/ml each ...
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bioRxiv - Cancer Biology 2024Quote: ... TL-1 cells were cultured in RPMI-1640 with 10% FBS and supplemented with 200 U/mL IL-2 (Miltenyi Biotec cat # 130-097-743) and NKL cells were cultured in RPMI-1640 with 10% FBS and supplemented with 100 U/mL IL-2.
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bioRxiv - Genomics 2023Quote: ... 2 mM EDTA; dilution of MACS BSA Stock Solution [Miltenyi Biotec, 130-091-376] 1:20 with autoMACS® Rinsing Solution [Miltenyi Biotec, 130-091-222]).
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bioRxiv - Immunology 2021Quote: ... and incubated for 45 mins at 4°C on a MACSmix Tube Rotator (Miltenyi Biotec). Anti-CD24 antibody-bound cells were then magnetically removed ...
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bioRxiv - Bioengineering 2021Quote: ... for 15 mins at 4°C and separated using a MACS Pro Separator (Miltenyi Biotec). Monocytes were cultured using Iscove’s Modified Dulbecco’s media supplemented with 10% human serum blood type AB ...
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bioRxiv - Physiology 2022Quote: ... for 20 minutes at 4°C before Auto MACS cell separation (Miltenyi Biotec, Cologne, Germany). Cells were then sorted with a BD FACSAria Fusion (BD Biosciences ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... falciparum culture (4% hematocrit, 5-10% parasitemia) was passed through an LS column (Miltenyi Biotec; previously rinsed with 2 mL culture medium ...
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bioRxiv - Bioengineering 2023Quote: ... Tumor digestion protocol #4 was performed by using the gentleMACS Octo tissue dissociator (Miltenyi Biotec) with three 10 min rounds of processing over 40 min ...
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bioRxiv - Immunology 2024Quote: ... with the anti-CD304 (BDCA-4/Neuropilin-1) MicroBead kit (Miltenyi Biotec, Bergisch-Gladbach, Germany) and identified as BDCA-2 positive ...
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bioRxiv - Neuroscience 2024Quote: ... M2 macrophages were generated by conditioning BMDM with IL-4 (20 ng/ml; Miltenyi Biotec) for 16-18 hours at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... and resuspended in cold selection buffer (PBS + 0.5% BSA + 2 mM EDTA) mixed with 20% (v/v) CD20 MicroBeads (Miltenyi Biotec #130-091-104 and #130-042-201) for 15 min at 4 ºC ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were stained for 15min at 4°C and then transferred to LS columns (Miltenyi Biotec). Subsequently ...
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Single cell transcriptomics reveals the effect of PD-L1/TGF-β blockade on the tumor microenvironmentbioRxiv - Genomics 2020Quote: ... and the tissue was stored overnight at 4°C in MACS Tissue Storage Solution (Miltenyi Biotec). Tumor tissue was minced and dissociated using the mouse Tumor Dissociation Kit (Miltenyi Biotec ...
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bioRxiv - Systems Biology 2023Quote: ... incubated for 15 minutes at 4°C and transfered in conditioned Whole Blood Columns (Miltenyi Biotec) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Intracellular markers (OCT3/4, NANOG) were stained by fixation/permeabilization solution (Miltenyi Biotec, 130-093-142) (30 min ...
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bioRxiv - Neuroscience 2023Quote: ... At d15 cells were treated with IL-4 (20 ng/ml, LOT130-094-117, Miltenyi Biotec), IL-6 (100 ng/ml ...
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bioRxiv - Immunology 2024Quote: ... supplemented with 1000 IU/ml GM-CSF and 400 IU/ml IL-4 (both Miltenyi Biotec) for 5 days ...
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bioRxiv - Molecular Biology 2023Quote: ... for monocyte-derived dendritic cells and 20 ng/ml IL-4 (#130-093-920, Miltenyi Biotec) and 40 ng/ml M-CSF (#130-096-485 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were passaged (1:12) every 3-4 days using StemMACS Passaging Solution XF (Miltenyi Biotec) and Y-27632 dihydrochloride (Santa Cruz ...
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bioRxiv - Neuroscience 2024Quote: ... The cell pellet was then resuspended in 100 µl of cold D-PBS containing fluorochrome-conjugated antibodies recognizing mouse CD11b and ACSA-2 (Miltenyi Biotec, 130-113-810 and 130-116-247) in a 1:100 dilution and incubated for 10 min at 4°C in the dark ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were cultured in clusters and passaged every 4-5 days using StemMACS Dissociation reagent (Miltenyi Biotec). All iPS lines were genotyped using an RFLP at the relevant important loci and the sequence was confirmed by Sanger sequencing ...
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bioRxiv - Immunology 2021Quote: ... cut into 4 pieces and enzymatically digested by using the whole skin dissociation kit from Miltenyi biotec without enzyme P for 5 hours at 37°C under agitation (500 rpm) ...
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bioRxiv - Immunology 2021Quote: ... Fc receptors were blocked for 10 minutes at 4°C with FcR blocking reagent (MACS Miltenyi Biotec). Cells harvested during the conversion period from CD34+ hematopoietic precursors to CD14+ monocytes (Days 12-21 of the differentiation protocol ...
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bioRxiv - Immunology 2021Quote: ... in the presence of 50 ng/ml GM-CSF and 20 ng/ml IL-4 (Miltenyi Biotec). Untouched peripheral blood cDC1 and cDC2 (cDCs ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were stained for 15min at 4°C and then transferred to an LS column (Miltenyi Biotec). Human cells were then cytospinned with Shandon cytofunnels (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed with HBSS and spun at 300 g for 5min at 4 °C (Miltenyi Biotec). The pellet was resuspended and incubated with 80 µL of MACS buffer and 20 µL of anti-IgM microbeads (Miltenyi Biotec ...
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bioRxiv - Plant Biology 2024Quote: ... 4% BSA) and incubated in a primary antibody solution anti-GFP-HRP (1/5000, MACS Miltenyi Biotec) and 1/5000 anti-His (11667475001 ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were incubated at 4 with magnetic microbeads conjugated to monoclonal anti-human CD14 Abs (Miltenyi Biotec) and isolated by positive magnetic selection according to manufacturer’s instructions (Miltenyi Biotec) ...
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bioRxiv - Immunology 2024Quote: ... and stored at 4°C before being acquired using the MACSQuant Analyzer 10 flow cytometer (Miltenyi Biotec) and analyzed using FlowJo 10 ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were then incubated with anti-APC magnetic beads (Miltenyi Biotec, Catalog No. 130-090-855, 1/4) for 20 min at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... tumor portions were minced to 3-4 mm pieces and dissociated using a Tumor Dissociation Kit (Miltenyi Biotec), followed by the clearance of red blood cells with RBC lysis buffer (eBioscience ...
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bioRxiv - Genetics 2020Quote: ... following the manufacturer’s instruction for 30 minutes (4 °C) before being passed successively through two LS Columns (Miltenyi Biotec) with three MACs buffer washes ...
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bioRxiv - Microbiology 2020Quote: ... and the medium on the monocytes was replenished with X-vivo 15 + L-glutamine containing interleukin-4 (Miltenyi Biotec) and granulocyte-macrophage colony-stimulating factor (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2021Quote: ... sliced into 4 sagittal sections and placed into gentleMACS C-tubes (#130-093-237, Miltenyi Biotec, San Diego, CA), and processed for generation of single-cell suspensions using the Adult Brain Dissociation Kit and GentleMACS Octo Dissociator system (#130-107-677 and #130-095-937 ...
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bioRxiv - Immunology 2020Quote: ... The proteins in the supernatant were diluted to 4 mg/mL and analyzed using MACSplex Human cytokine (Miltenyi Biotec) and data were analyzed with FlowLogic 7.3 software.
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bioRxiv - Physiology 2023Quote: ... at 4°C for 15 min under continuous agitation and passed through MS column (130-042-201, Miltenyi Biotec). The CD146 positive cells ...
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bioRxiv - Cancer Biology 2022Quote: ... and 37°C incubator and passaged every 4-5 days in small clusters using StemMACS Dissociation reagent (Miltenyi Biotec). Colon organoids were differentiated as previously described[46] with minor modifications ...
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bioRxiv - Genomics 2024Quote: ... 4°C) with the surface antibody CD34-PE-Vio770 (clone AC136, lot no. 5180718070, dilution 1:50, Miltenyi Biotec) and DAPI (Sigma-Aldrich) ...
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bioRxiv - Immunology 2022Quote: ... Next the cells were washed and incubated for 20 min at 4°C with 100μl anti-FITC MACS magnetic beads (Miltenyi Biotec) per 108 cells ...