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Citations for Miltenyi Biotec :
351 - 400 of 1126 citations for 1 5 Bromo 1 2 Trimethylsilyl Ethoxy Methyl 1H Pyrrolo 2 3 B Pyridin 3 Yl Ethanone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... Conjugated primary antibodies used was anti-cardiac troponin 2 (TNNT2) PE (Miltenyi Biotec – 130-120-405). Viability marker Zombie Aqua (Biolegend – 423102 ...
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bioRxiv - Neuroscience 2023Quote: ... and 2 EAE late stage samples were processed debris removal solution (Miltenyi Biotec, 130-107-677) according to the manufacturer’s protocol to enrich more OPCs ...
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bioRxiv - Molecular Biology 2023Quote: ... and incubated in dissociation solution (Multi Tissue Dissociation Kit 2; Miltenyi Biotec; Catalog # 130-110-203) for 30 minutes at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... in a six-well plate in 2 ml StemMACS iPS-Brew (Miltenyi Biotec, Bergisch Gladbach, Germany). Medium was supplemented with 100 U/ml penicilin/streptomycin (Gibco ...
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bioRxiv - Immunology 2021Quote: ... CD8 T cells from spleens and lymph nodes of either P14 or FSF-Tim-3/E8i-Cre/P14 mice were purified by magnetic separation using a naïve CD8+ T cell isolation kit (Miltenyi Biotec #130-104-075) and then mixed with gp33-pulsed B cells at a ratio of 1:1 and incubated for indicated times at 37°C.
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bioRxiv - Molecular Biology 2023Quote: ... The protein complexes were then eluted into 1.5 mL Eppendorf tubes using 3 x 35 µL of pre-heated 95°C elution buffer (Miltenyi Biotec #130-042-701). The samples were heated to 95°C for 5 min ...
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bioRxiv - Bioengineering 2024Quote: ... were obtained after transduction of Jurkat T cells with a serial dilution of viral supernatants and transduction efficiency evaluated after 3-4 days by monitoring cell surface dNGFR expression using an anti-CD271-APC antibody (Miltenyi Biotec., Paris, France) or HLA-A*02 Dextramer-APC (Immudex ...
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bioRxiv - Immunology 2021Quote: ... were isolated by gradient centrifugation and then enriched for B cells by negative selection using a pan-B-cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec, 130-101-638).
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bioRxiv - Immunology 2021Quote: ... peripheral blood mononuclear cells were enriched for B cells by negative selection using a pan-B-cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were incubated in FACS buffer (1× PBS ...
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bioRxiv - Immunology 2021Quote: ... peripheral blood mononuclear cells were enriched for B cells by negative selection using a pan-B-cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were incubated in Flourescence-Activated Cell-sorting (FACS ...
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bioRxiv - Immunology 2022Quote: ... peripheral blood mononuclear cells were enriched for B cells by negative selection using a pan-B-cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were incubated in Flourescence-Activated Cell-sorting (FACS ...
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bioRxiv - Immunology 2022Quote: ... peripheral blood mononuclear cells were enriched for B cells by negative selection using a pan-B-cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were incubated in FACS buffer (1× PBS ...
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bioRxiv - Immunology 2022Quote: ... peripheral blood mononuclear cells were enriched for B cells by negative selection using a pan-B-cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were incubated in Flourescence-Activated Cell-sorting (FACS ...
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bioRxiv - Immunology 2021Quote: ... peripheral blood mononuclear cells were enriched for B cells by negative selection using a pan-B-cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were incubated in FACS buffer (1× PBS ...
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bioRxiv - Immunology 2021Quote: Murine naïve B cells were purified from spleen by magnetic negative selection by using Pan B Cell Isolation Kit (Miltenyi Biotec, 130-095-813) according to the manufacturer’s instructions and maintained in appropriate culture medium ...
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bioRxiv - Immunology 2020Quote: PBMCs were enriched for B cells by negative selection using a pan B cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were incubated in FACS buffer (1 X Phosphate-buffered Saline (PBS) ...
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bioRxiv - Immunology 2022Quote: ... were enriched for B cells by negative selection using a pan-B-cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec, 130-101-638). The enriched B cells were incubated in Flourescence-Activated Cell-sorting (FACS ...
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bioRxiv - Immunology 2021Quote: ... A total of 1.5 × 106 PBMCs were stimulated with 1 µg/mL of peptide pools for 6 h in TexMACS™ GMP Medium (Miltenyi Biotec), supplemented with 5% AB human serum (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... mouse B cells were first isolated from spleen and lymph nodes using the Pan B-cell Isolation Kit II (Miltenyi Biotec,130-104-443) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... mice were sacrificed and B cell were isolated from spleen and lymph nodes with mouse Pan B Cell Isolation Kit II (Miltenyi Biotec, 130-104-443) and LS columns (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: Splenic B cells from 24-week old MRL/lpr mice were isolated with a Pan B Cell Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany). B cells were cultured with RPMI-1640 medium (Lonza ...
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bioRxiv - Immunology 2023Quote: Single-cell suspensions of splenocytes from individual mice were prepared and B cell enrichment was performed using the Pan B Cell Isolation Kit (Miltenyi Biotec, #130-095-813). Plasmablast cell sorting was performed using a FACSAria (BD Biosciences) ...
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bioRxiv - Bioengineering 2024Quote: ... frozen liver samples were added to ice-cold PBS at 50 mg/mL and processed for tissue homogenization using the gentleMACS Dissociator (n = 3 per group) (Miltenyi Biotec, Bergisch Gladbach, Germany). Then ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.4 x 106 isolated T cells from the same donor and 0.4 x 106 T cell activation beads (Miltenyi Biotec, anti-CD2/3/28) were added ...
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bioRxiv - Cell Biology 2024Quote: ... the pellet from each BM sample was resuspended in 392µl PBS/3% FBS and 8µl CD117 (cKit) MicroBeads (Miltenyi Biotec, catalog number 130-091-224) and incubated on ice for 30 mins ...
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bioRxiv - Immunology 2020Quote: ... B cells were additionally permeabilized with Inside Perm (Miltenyi Biotec) and stained with the unlabeled primary antibodies anti-NET ...
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bioRxiv - Immunology 2021Quote: Splenocytes or lung leukocytes were incubated with SARS-CoV-2 S peptide pools (1μg/ml, Miltenyi Biotec) for 24 h ...
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bioRxiv - Genomics 2021Quote: ... 2 µl of the respective antibodies were used: FITC anti-human CD10 (Miltenyi Biotec, #130-124-262) and APC anti-human CD99 (Miltenyi Biotec ...
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bioRxiv - Bioengineering 2020Quote: ... mouse anti-human ErbB-2 (CD340, HER2) Vio® Bright FITC (130-121-436) from Miltenyi Biotec; and rabbit anti-human HER2 mAb (2165S ...
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bioRxiv - Synthetic Biology 2024Quote: ... The cells were incubated with PBS supplemented with 2 μg/ml DAPI (Miltenyi Biotec, 130-111-570) for 15 minutes at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... with Fc receptors blocking prior to Anti-ACSA-2 MicroBeads (Cat# 130-097-678, Miltenyi Biotec, Germany). Incubation was carried out for 15 minutes in the refrigerator at 4℃ ...
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bioRxiv - Microbiology 2023Quote: Splenocytes or lung leukocytes were incubated with SARS-CoV-2 spike peptide pools (1μg/ml, Miltenyi Biotec) for 6 h in the presence of GolgiPlug (BD Bioscience) ...
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bioRxiv - Molecular Biology 2023Quote: ... Single-cell suspensions were prepared using Multi Tissue Dissociation Kit 2 (Miltenyi Biotec; Catalog # 130-110-203) following the instructions provided with the kit ...
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bioRxiv - Molecular Biology 2023Quote: ... The samples were then incubated with 2 mL of diluted RBC lysis buffer (Miltenyi Biotec, Auburn, CA) for 15 minutes at room temperature ...
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bioRxiv - Immunology 2024Quote: ... lymphoid organ cells were pulsed with a SARS-CoV-2 peptide pool (Miltenyi Biotec, # 130-127-309) (1 μg/mL ...
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bioRxiv - Immunology 2024Quote: ... CD8+ T cells were positively stained using CD8a (Ly-2) microbeads (Miltenyi Biotec, cat. # 130-117-044) and the negative flow-through fraction was collected ...
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bioRxiv - Cancer Biology 2024Quote: ... with a final concentration of 50 IU/mL of IL-2 (Miltenyi Biotec catalog #130-097-743). Cell-free supernatants were collected ...
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bioRxiv - Developmental Biology 2023Quote: ... and incubated the samples for a total of 1h at RT with intermittent pulses of mechanical tissue disruption using the gentleMACSTM tissue homogenizer (Miltenyi Biotec). The resulting homogenate was washed twice using 10 ml of ice-cold 1% BSA/PBS and pelleted for 5min at 800g (4°C) ...
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bioRxiv - Bioengineering 2024Quote: ... chambers were obtained from the Stanford Blood Center and primary human B cells were isolated by negative selection using the human B Cell Isolation Kit II (Miltenyi Biotec, cat: 130-091-151). Cells were cultured in Iscove’s modified Dulbecco’s medium (IMDM ...
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bioRxiv - Cell Biology 2024Quote: ... NM + 1 μM RA + 1 μΜ Purmorphamine (Miltenyi Biotec 130-104-465), was added and changed every other day until D17 ...
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bioRxiv - Microbiology 2022Quote: ... after which 3 mL of FACS buffer was added and the mixture was passed over a magnetized LS column (Miltenyi Biotec, cat#130–042– 401). The column was washed once with 5 mL ice-cold FACS buffer and then removed from the magnetic field and 5 mL ice cold FACS buffer was pushed through the unmagnetized column twice using a plunger to elute the bound cell fraction.
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bioRxiv - Immunology 2021Quote: ... The cell fraction was then depleted with human CD45 beads (100ul beads per 2×108 cells; Miltenyi Biotec) using autoMACS ...
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bioRxiv - Immunology 2020Quote: ... covering the N-terminal S1 domain of the Spike protein of SARS-CoV-2 (Miltenyi Biotec, Auburn, CA) at 0.2 μg/mL and 0.5 μg/mL per peptide ...
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Activation of innate immune cGAS-STING pathway contributes to Alzheimer’s pathogenesis in 5×FAD micebioRxiv - Neuroscience 2022Quote: Cell samples were fluorescently stained with Anti-ACSA-2-PE (clone: IH3-18A3) (130-102-365, Miltenyi Biotec), examined by a BD FACSCanto II flow cytometer ...
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bioRxiv - Physiology 2022Quote: Primary cardiac fibroblasts were isolated from 2-day-old rats using a neonatal heart dissociation kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... lungs were harvested in 2 ml of Trizol and homogenized using a gentleMACS Octo dissociator (Miltenyi Biotec, Inc.). Total RNA was purified with trizol/chloroform ...
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bioRxiv - Neuroscience 2023Quote: ... Enzyme Mix 2 (15 μl of Buffer Y, 7.5 μl of Enzyme A [Miltenyi Biotec, 130-094-802]) was added for another 5-min ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 and 4 cytokines were added as followed: 20 ng/ml IL-4 (#130-093-920, Miltenyi Biotec) and 100 ng/ml GM-CSF (#130-093-864 ...
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bioRxiv - Immunology 2023Quote: ... and rested overnight with TexMACS medium supplemented with 100 IU/ml of recombinant human IL-2 (Miltenyi Biotec).
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bioRxiv - Immunology 2020Quote: B cells were isolated from PBMCs using CD19-microbeads (Miltenyi Biotec) according to the manufacturer’s instruction ...