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Citations for Miltenyi Biotec :
301 - 350 of 578 citations for TMEM173 Human HEK293 Sumo His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... CD14+ monocytes were then obtained by positive magnetic isolation using human CD14 microbeads (Miltenyi Biotec) and a MACS LS column with a QuadroMacs™ separator (Miltenyi Biotech) ...
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bioRxiv - Cancer Biology 2023Quote: ... and cultured in cRPMI supplemented with 5,000 U/ml recombinant human IL-2 (Miltenyi Biotec). Mouse NK (mNK ...
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bioRxiv - Cancer Biology 2024Quote: ... The freshly isolated PBMCs were subsequently cultured in human NK MACS® medium (Miltenyi Biotec) for 2 to 3 weeks to ensure optimal in vitro expansion of NK cells ...
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bioRxiv - Biochemistry 2019Quote: ... and subsequent CD14+ positive selection using the MACS Miltenyi Biotec Human CD14 microbead protocol (Miltenyi Biotec). CD14+ cells were differentiated in to macrophages using recombinant human granulocyte-macrophage colony-stimulating factor (200 ng/mL GM-CSF ...
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bioRxiv - Cell Biology 2021Quote: ... and 60 ng/mL of human receptor activator of NF-κB-ligand (RANK-L, Miltenyi Biotec). The medium was then changed every third day by RPMI containing 10% FCS ...
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bioRxiv - Cell Biology 2020Quote: ... or 20 ng/ml PDGF-AA (human PDGF-AA, Cat nb. 130-108-983, Miltenyi Biotec). Inhibition of PDGF-signaling was performed by addition of 1μM imatinib (Cat nb ...
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bioRxiv - Neuroscience 2022Quote: ... the cells were labeled with human CD11b-APCVio770 and CD45-phycoerythrin (PE) (Miltenyi Biotec, Gladbach, Germany), and flow cytometry was performed as described previously (38) ...
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bioRxiv - Microbiology 2022Quote: ... Bacteria were stained with PE Anti-human IgA antibodies (1:100; Miltenyi Biotec clone IS11-8E10) for 30 min on ice ...
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bioRxiv - Immunology 2020Quote: ... T cells were isolated from PBMC using the Human Pan T cell isolation kit (Miltenyi Biotec) and subsequently activated by TransAct (Miltenyi Biotec) ...
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bioRxiv - Immunology 2020Quote: ... The cells were isolated using a human negative selection-based NK isolation kit (RosetteSep, Miltenyi Biotec). The purified NK cells were then cultured in a stem cell serum-free growth medium (CellGenix GMP SCGM ...
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bioRxiv - Immunology 2022Quote: ... B cells were first isolated from donors’ PBMC by MACS using human CD19 MicroBeads (Miltenyi Biotec). CD19+ B cells were then stained using LIVE/DEAD aqua fixable dead cell stain kit (Molecular Probes ...
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bioRxiv - Immunology 2022Quote: Breast tissue was enzymatically and mechanically dissociated as per the Human Tumor Dissociation Kit (Miltenyi Biotec) protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... we also used CD3-PE to specifically identify lymphocytes (human antibody, clone BW264/56, Miltenyi Biotec). For all the samples ...
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bioRxiv - Immunology 2020Quote: ... CD14+ cells were isolated using the MACS human CD14+ isolation kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2020Quote: ... and the media was further supplemented with 100 U/ml human IL-2 IS (Miltenyi Biotec), 1 ng/ml IL-15 (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2020Quote: ... Primary human CD4+ T cells were isolated by untouched CD4+ T cell isolation kit (Miltenyi Biotec). Human CD4+ T cells were activated by Dynabeads Human T-Activator CD3/CD28 (ThermoFisher ...
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bioRxiv - Immunology 2020Quote: For cultured human lung macrophages staining: anti-CD169 (7-239) and anti-CD11c (REA618, Miltenyi Biotec) were used for membrane staining ...
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bioRxiv - Immunology 2020Quote: ... CD8+ T cells were purified from PBMC via human CD8 MicroBeads (Miltenyi Biotec, 130-045-201) and activated with T Cell TransAct (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... CD3 selection was performed after being transduced with γδTCR-Cl5 using human CD3 microbeads (Miltenyi Biotec). Moreover ...
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bioRxiv - Immunology 2020Quote: ... Immature human moDCs were generated from PBMCs using CD14 Microbeads (Miltenyi Biotec cat. 130-050-201) and cultured for 6 days with recombinant GM-CSF (R&D Systems cat ...
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bioRxiv - Microbiology 2022Quote: ... and human granulocyte-macrophage colony-stimulating factor (GM-CSF; 20ng/ml; MILTENYI BIOTEC; 130-093-866). At day 5 ...
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bioRxiv - Microbiology 2023Quote: ... CD4+ T cells were isolated by positive selection (using magnetic CD4 human MicroBeads from Miltenyi Biotec). Cells were activated with CD3/CD28 agonists (T Cell transact ...
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bioRxiv - Immunology 2024Quote: B cells were eluted from PBMCs using a MACS Human B Cell isolation kit (Miltenyi Biotec). B cells were stained with rGP38 (IbAr10200 ...
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bioRxiv - Immunology 2021Quote: Naive B cells were purified from PBMCs using the MACS Human B Cell isolation kit (Miltenyi Biotec) and incubated with 25nM of each SARS-CoV-2 probe (RBD-mFc-BV650 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Primary human T cells were purified from PBMCs by Pan T Cell Isolation Kit (Miltenyi Biotec, Germany). Purified T cells were stimulated with anti-CD3/CD28 Dynabeads (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... CD14+ monocytes were isolated from PBMCs using human anti-CD14 magnetic beads with LS Columns (Miltenyi Biotec). CD14+ monocytes were differentiated into MDM in RPMI 1640 medium supplemented with 10% FBS ...
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bioRxiv - Immunology 2020Quote: ... NK cells were isolated from PBMCs by negative depletion with human NK cell Isolation Kit (Miltenyi Biotec). Cells were cultured in RPMI 1640 medium supplemented with GlutaMAX (Gibco) ...
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bioRxiv - Cancer Biology 2022Quote: ... freshly isolated tumor samples were dissociated using the human tumor dissociation kit (Miltenyi Biotec; 130-095-929): surgical resection tissue was first minced into small pieces using a scalpel ...
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bioRxiv - Cancer Biology 2022Quote: ... 25 μL Enzyme A (all provided in the human tumor dissociation kit (Miltenyi Biotec; 130-095-929), and 4.7 mL DMEM (Gibco ...
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bioRxiv - Immunology 2022Quote: ... 100,000 cells per well were activated with anti-human TransAct (1:100, Miltenyi Biotec 130-111-160) in T cell medium (10 % FCS ...
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bioRxiv - Cancer Biology 2022Quote: ... Anti-mouse and anti-human FcyR blocking antibodies were used to prevent non-specific binding (Miltenyi Biotec). 7-aminoactinomycin D (BD ...
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bioRxiv - Cancer Biology 2022Quote: ... The xenograft tumors were immediately digested using the human Tumor Dissociation Kit (130-095-929, Miltenyi Biotec) in combination with the GentleMACS Octo Dissociator (Miltenyi Biotec) ...
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bioRxiv - Developmental Biology 2021Quote: ... This enriched macrophage/monocyte solution was incubated with human and mouse CD11b microbeads (Miltenyi Biotec; 130093-634), and cells were further enriched for Cd11b-positive macrophages/monocytes using Miltenyi MACS Separation Columns (LS ...
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bioRxiv - Neuroscience 2022Quote: ... Human iPSCs (hiPSCs) were cultured in a chemically defined growth medium (StemMACS iPS-Brew XF (Miltenyi Biotec) or mTSeRTM1 (Stem Cell Technologies ...
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bioRxiv - Immunology 2020Quote: ... in 5% human serum RPMI medium in the presence of 1µg/ml anti-CD40 (HB14, Miltenyi Biotec). After 3 hrs of specific peptide stimulation ...
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bioRxiv - Immunology 2021Quote: ... CD19+ B cells were purified from human PBMCs using the REAlease® CD19 Microbead Kit (Miltenyi Biotec) according to the manufacturer’s recommendations with purity greater than 98 %.
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bioRxiv - Genomics 2020Quote: Dissociation mixture was based on Miltenyi Human Tumor Dissociation Kit (Miltenyi Biotec, catalog no. 130-095-929). Before starting ...
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bioRxiv - Genomics 2021Quote: ... 2 µl of the respective antibodies were used: FITC anti-human CD10 (Miltenyi Biotec, #130-124-262) and APC anti-human CD99 (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were stained with PE/Cy7 anti-human CD44 antibody (Miltenyi Biotec, 130-113-904, 1:50) and CD338-PE (ABCG2-PE ...
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bioRxiv - Microbiology 2020Quote: ... 50 ng/ml recombinant human granulocyte macrophage colony–stimulating factor (rhGM-CSF) (130-093-865, Miltenyi Biotec), and 50 ng/ml rh interleukin (IL)-4 (130-093-921 ...
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bioRxiv - Immunology 2020Quote: ... iMS1 and iMono cells were isolated after 7 d of culture using human CD14 Microbeads (Miltenyi Biotec). Isolated CD14+ cells were validated using flow cytometry (CD14-FITC ...
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bioRxiv - Immunology 2021Quote: ... Anti-Biotin multi-sort and human CD4+CD45RO+ isolation kits were purchased from Miltenyi Biotec (Auburn, CA). PE+ cell ...
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bioRxiv - Immunology 2021Quote: ... total PMBCs were enriched for B cells using a MACS human B cell isolation kit (Miltenyi Biotec). Purified B cells were enumerated ...
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bioRxiv - Bioengineering 2020Quote: ... mouse anti-human ErbB-2 (CD340, HER2) Vio® Bright FITC (130-121-436) from Miltenyi Biotec; and rabbit anti-human HER2 mAb (2165S ...
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bioRxiv - Molecular Biology 2022Quote: ... the human induced pluripotent stem cell (hiPSC) line HMGU12 was harvested using StemMACS Passaging Solution (Miltenyi Biotec) and resuspended in DMEM/F-12 medium supplemented with 20% KSR ...
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bioRxiv - Cell Biology 2022Quote: ... Human peripheral blood monocytes were isolated by the classical monocyte isolation kit (Miltenyi Biotec, #130-117-337), according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... dissociated cells were incubated with biotin conjugated anti-human CD171(L1CAM) (Miltenyi Biotec, Cat#130-124-046) in MACS buffer at 4°C for 10 min ...
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bioRxiv - Immunology 2023Quote: iMSC-like cells were acquired by stimulating ADSCs with recombinant human IL-1β (0.5ng/ml, Miltenyi Biotec) overnight ...
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bioRxiv - Biophysics 2023Quote: ... Human primary classical monocytes (CD14+CD16-) were further isolated using the Classical Monocyte Isolation Kit (Miltenyi Biotec) by magnetic-activated cell sorting (MACS) ...
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bioRxiv - Immunology 2023Quote: Human CD4+ T cells were isolated by employing a CD4+ T cells isolation kit (Miltenyi Biotec kit) following the provider’s instructions ...