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Citations for Miltenyi Biotec :
301 - 350 of 726 citations for Recombinant Human CD274 Biotin His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... and CD81 antibodies (Human Exosome Isolation Kit, Pan, Miltenyi Biotec) followed by EV isolation via magnetic separation and elution ...
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bioRxiv - Genetics 2022Quote: ... Then cells were incubated with human CD34 Microbeads (Miltenyi Biotec) and Fc receptor blocker in MACS buffer (1% BSA/2mM EDTA/PBS ...
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bioRxiv - Cancer Biology 2019Quote: ... we used CD45-VioBlue (human antibody, clone REA747, Miltenyi Biotec) to identify immune cells and in few cases ...
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bioRxiv - Genetics 2021Quote: ... Human CD14+ monocytes Isolation kit (Miltenyi Biotec, 130-050-201) and Human CD19+ B Cell Isolation kit (Miltenyi Biotec,130-050-301 ...
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bioRxiv - Molecular Biology 2021Quote: ... PBMCs were incubated with anti-human CD14 MicroBeads (Miltenyi Biotec) and monocytes were separated on a LS magnetic column on a QuadroMACS separator (Miltenyi Biotec) ...
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bioRxiv - Genomics 2021Quote: ... and APC anti-human CD99 (Miltenyi Biotec, #130-121-096). Cells were incubated for 15 min on ice and protected from light ...
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bioRxiv - Genomics 2021Quote: ... OctoMACS separator and CD19 MicroBeads (human) (all from Miltenyi Biotec) modifying slightly the data sheet protocol according to the experimental needs and the cell types (figure 1).
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bioRxiv - Cancer Biology 2022Quote: ... in FACS buffer containing 1% human Fc Block (Miltenyi Biotec) for 30 mins at 4ºC and were then washed three times ...
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bioRxiv - Systems Biology 2023Quote: ... Monocytes were positively selected using Human CD14 MicroBeads (Miltenyi Biotec) and LS Columns ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-human GLAST (ACSA-1) biotinylated antibody (Miltenyi Biotec, Inc. ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 100 U/ml human IL-2 (Miltenyi Biotec) and 100 U/ml Penicillin/Streptomycin (Gibco) ...
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bioRxiv - Cancer Biology 2023Quote: ... Human T Cell TransAct (Miltenyi Biotec t# 130-111-160) was used to activate T cells ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 100 U/ml human IL-2 IS (Miltenyi Biotec), 1 ng/ml IL-15 (Miltenyi Biotec) ...
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bioRxiv - Cancer Biology 2023Quote: ... after treatment with a human FcR blocking reagent (Miltenyi Biotec). Flow cytometric analysis was performed as described above ...
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bioRxiv - Molecular Biology 2024Quote: A MACSPlex human Exosome kit (Miltenyi Biotec, Bergisch-Gladbach, Germany) was used ...
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bioRxiv - Microbiology 2024Quote: ... in the presence of human FcR blocking reagent (Miltenyi Biotec) and subsequent staining with anti-PE paramagnetic microbeads (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... CD14+ monocytes were isolated using Human CD14 Microbeads (Miltenyi Biotec). Cell purity was verified by flow cytometry ...
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bioRxiv - Immunology 2022Quote: TILs were stimulated for 7 days with anti-biotin MACSiBead Particles loaded with biotinylated CD3/CD28 antibodies (Miltenyi Biotec, Bergisch Gladbach, Germany). The preparation of the conjugated beads was carried out according to the manufacture’s recommendation (activation/expansion kit ...
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bioRxiv - Molecular Biology 2023Quote: ... natural killer cells and monocytes were isolated from PBMCs by negative selection and magnetic sorting using cocktails of biotin-conjugated antibodies against non-target cells (#130-096-535,Miltenyi Biotec for T cells, (#130-101-638, Miltenyi Biotec for B cells ...
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bioRxiv - Bioengineering 2024Quote: ... chambers were obtained from the Stanford Blood Center and primary human B cells were isolated by negative selection using the human B Cell Isolation Kit II (Miltenyi Biotec, cat: 130-091-151). Cells were cultured in Iscove’s modified Dulbecco’s medium (IMDM ...
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bioRxiv - Bioengineering 2022Quote: ... Non-plasma cells were labeled with a biotin-conjugated antibody cocktail combined with anti-biotin microbeads and separated using a magnetic rack according to the manufacturer’s protocol (Miltenyi Biotec, 130-092-530). Purified plasma B cells were eluted and sequentially incubated with CD138 microbeads for an additional 15 minutes at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... The remaining pellet was resuspended in 80 μL of 0.5% BSA and 20 μL of Anti-Biotin MicroBeads (Miltenyi Biotec, # 130-115-389) and incubated for 10 minutes at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... The remaining pellet was resuspended in 80 μL of 0.5% BSA and 20 μL of Non-Neuronal Cells Biotin-Antibody Cocktail (Miltenyi Biotec, # 130-115-389) and incubated for 5 minutes at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed in complete media and CD45+ cells isolated using CD45 magnetic bead separation for non-human primates (Miltenyi Biotec CD45 Microbeads non-human primate) in accordance with the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed in complete media and CD45+ cells isolated using CD45 magnetic bead separation for non-human primates (Miltenyi Biotec CD45 Microbeads non-human primate) in accordance with the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were enzymatically dissociated using Human Tumor Dissociation Kit (Miltenyi Biotec) according to manufacturer’s protocol (https://www.miltenyibiotec.com/AU-en/products/macs-sample-preparation/tissue-dissociation-kits/tumor-dissociation-kit-human.html#gref) ...
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bioRxiv - Neuroscience 2022Quote: ... human BDNF (100 ng/mL, MACS Miltenyi Biotec, 130-096-286), LM22A4 (5 µM ...
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bioRxiv - Immunology 2022Quote: ... using the human CD4+ T cell isolation kit (MACS Miltenyi Biotec). Isolated T helper cell purity was checked by flow cytometry CD4 staining and they were then cultured in CD3 (5µg/ml ...
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bioRxiv - Microbiology 2022Quote: ... and 100 IU/ml human interleukin 2 (IL-2, Miltenyi Biotec). Ten micromolar integrase inhibitor ...
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bioRxiv - Neuroscience 2021Quote: ... 10 ng/ml human M-CSF (130-096-491, Miltenyi Biotec), 10 ng/mL GM-CSF (130-093-862 ...
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bioRxiv - Immunology 2020Quote: Human nTregs were CFSE labelled and cultured in TexMACs (Miltenyi Biotec) supplemented with 10% heat inactivated fetal bovine serum (HI-FBS ...
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bioRxiv - Systems Biology 2019Quote: ... antibodies in the presence of human FcR Blocking Reagent (Miltenyi Biotec) at 4°C for 15 mins ...
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bioRxiv - Cancer Biology 2019Quote: ... The tissues were dissociated with Human Tumor Dissociated kit (Miltenyi Biotec) and filtered using 100µm SmarterStrainers (Miltenyi Biotec) ...
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bioRxiv - Immunology 2020Quote: ... incubated with human FcR Block (Miltenyi Biotec, Cat#130-059-901) at 1/70 in PBS-FBS 1% for 10 min ...
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bioRxiv - Immunology 2020Quote: ... Mouse anti-human PE anti-CD107a mAb (REA792, from Miltenyi Biotec) was added and cells were cultured at 37°C for one hour ...
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bioRxiv - Immunology 2020Quote: ... Monocytes were isolated from PBMC with human CD14 MicroBeads (Miltenyi Biotec) MACS isolation using manual separation with LD columns (Miltenyi Biotec) ...
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bioRxiv - Microbiology 2022Quote: ... and 100 IU/ml human interleukin 2 (IL-2, Miltenyi Biotec). All HTLV-1 infected T-cell clones were maintained in 10 µM of the integrase inhibitor ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Primary cells were blocked with human FcR blocking reagent (Miltenyi Biotec) and mouse-derived samples were additionally blocked with mouse FcR-blocking reagent (Miltenyi Biotec) ...
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bioRxiv - Bioengineering 2023Quote: ... CD31 antibody (anti-human, APC, REAfinityTM; 130-110-808; Miltenyi Biotec) [in 1:35 working concentration] and Hoechst 33342 [in 1:250 working concentration] were diluted in ECGM ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Monocytes were magnetically sorted using human CD14+ microbeads (Miltenyi Biotec, USA) according to manufactures protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were suspended in human FC block (Miltenyi Biotec Inc., FLP3330) and kept on ice for 30 minutes ...
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bioRxiv - Genomics 2023Quote: ... The tissues were digested with Human Tumor Dissociation Kit (Miltenyi Biotec) according to manufacturer’s manual ...
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bioRxiv - Cancer Biology 2024Quote: ... using the human tumor dissociation kit (Miltenyi Biotec, 130-095-929). Dead cells were removed using the dead cell removal kit (Miltenyi Biotec ...
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bioRxiv - Bioengineering 2024Quote: ... were stimulated with TransAct Human T cell activation reagent (Miltenyi Biotec), 100 U/ml human IL-2 IS (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2024Quote: ... and FITC anti-human CD3 (1:50; REA613 clone; Miltenyi Biotec) antibodies.
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bioRxiv - Cancer Biology 2021Quote: ... αβ T cells were transduced with pMP71: γ-T2A-δ and incubated with a biotin-labelled anti-αβ TCR antibody (clone BW242/412; Miltenyi Biotec) followed by incubation with an anti-biotin antibody coupled to magnetic beads (anti-biotin MicroBeads ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 mM EDTA in PBS) for 15 minutes and after two washes the samples were incubated with anti-biotin-APCVio770 (Miltenyi Biotec #130-113-851), diluted 1:50 in FACS buffer for 15 minutes ...
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bioRxiv - Immunology 2021Quote: αβT cells transduced with γδTCR-Cl5 either alone or together with NKG2D wild type or the different NKG2D chimeras were incubated with a biotin-labeled anti-αβTCR antibody (clone BW242/412; Miltenyi Biotec, Bergisch Gladbach, Germany) and subsequently incubated with an anti-biotin antibody coupled to magnetic beads (anti-biotin MicroBeads ...
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bioRxiv - Cancer Biology 2022Quote: ... and efficiently depleted of non-target cells by addition of magnetic microbeads conjugated to mouse anti-biotin monoclonal IgG1 antibodies and passage through LS ferromagnetic columns (Miltenyi Biotec, 130-042-401). The flow through ...
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bioRxiv - Immunology 2022Quote: ... followed by incubation with anti-Biotin mAb microbeads to deplete αβT-cells by magnetic separation using AUTOMACS under depleteS protocol (Miltenyi Biotec, Biergisch Gladbach, Germany). DOT cells were generated from αβ-depleted PBMCs cultured in either 96-well plates or G-REX platform (Wilson Wolf Manufacturing) ...