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Citations for Miltenyi Biotec :
301 - 350 of 398 citations for Porcine Tissue Lysates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... spinal cord tissues were homogenized using the Adult Brain Dissociation Kit (mouse and rat, cat # 130-107-677, Miltenyi Biotec) and running the gentleMACS program 37C_ABDK_02 following the manufacturer instructions.
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bioRxiv - Genomics 2023Quote: Singlet rate was estimated on dissociated tissues using flow cytometry on a MACSQuant Analyzer 10 (Miltenyi Biotec, 130-096-343). Flow cytometry data was analyzed using Flowlogic v8 (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... Grafts were flushed with saline and digested with collagenase A and DNase I in a gentleMACS tissue dissociator (Miltenyi Biotec). Cells were stained with cocktails of fluorochrome-conjugated monoclonal antibodies including AF647- conjugated anti-mouse CD57 (polyclonal ...
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bioRxiv - Genomics 2024Quote: Adrenal gland neoplasm samples were dissociated with 25-30 ul of enzyme D Multi Tissue Dissociation Kit (MTDK) (Miltenyi Biotec) or enzyme A Neural Tissue Dissociation Kit P (NTDK ...
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bioRxiv - Cancer Biology 2021Quote: Freshly resected tumor or adjacent normal lung tissue was thoroughly chopped with surgical scissors and transferred to GentleMACS C Tubes (Miltenyi Biotec) containing 20 uL/mL Liberase TL (5 mg/ml ...
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bioRxiv - Developmental Biology 2019Quote: ... cortices from 1-3 embryos from 2-5 mothers were pooled and dissociated into single cell suspension using the MACS Neural Tissue Dissociation kit with papain (Miltenyi Biotec) according to manufacturer’s instructions ...
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Low basal expression and slow induction of IFITM3 puts immune cells at risk of influenza A infectionbioRxiv - Immunology 2019Quote: ... Para-tumour lung tissue samples from metastatic cancer or fibrosis patients were processed using a tumour dissociation kit (MACS Miltenyi Biotec) to isolate immune and epithelial cells ...
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bioRxiv - Neuroscience 2020Quote: ... were prepared from embryonic day E14.5 mice as described before [54] or from postnatal day 7-8 mouse brains using the MACS cell separation technology using the Neural Tissue Dissociation Kit and anti-O4 MicroBeads (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... The tumor-bearing hemispheres and corresponding hemispheres from naïve animals were dissociated enzymatically to obtain a single-cell suspension with a Neural Tissue Dissociation Kit with papain (Miltenyi Biotec) or 0.5 mg/mL DNase I (DN25 ...
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bioRxiv - Neuroscience 2020Quote: Micro-dissected dentate gyri were cut into pieces with a scalpel blade and enzymatically dissociated using the Neural Tissue Dissociation Kit P (Miltenyi Biotec) according to the manufacturer’s manual ...
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bioRxiv - Cell Biology 2020Quote: ... from control and CL- and cold-treated mice were collected after CL treatment and processed for SVC isolation using mouse adipose tissue dissociation kit (Miltenyi biotec) according to manufactures.
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bioRxiv - Cancer Biology 2021Quote: ... the index tumor lesions (the most extensive with the highest Gleason score) were immediately harvested and transferred to precooled MACS tissue storage solution (Miltenyi Biotec) and shipped at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Tumours were digested using a mouse tumour dissociation kit in GentleMACS C digestion tubes with a GentleMACS tissue dissociator (Miltenyi Biotec). Enzyme activity was neutralised by addition of cold RPMI/2% FBS and suspension was dispersed through a 70 µm cell strainer ...
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bioRxiv - Cancer Biology 2022Quote: Fresh tumor tissue was minced to 2-4 mm sized pieces in a 6-cm dish and subsequently digested to single cell suspension using the Multi Tissue Human Tumor Dissociation Kit 1 (Miltenyi Biotec) and a gentleMACS OctoDissociator (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2022Quote: ... and fresh tissue was minced to 2-4 mm sized pieces in a 6-cm dish and subsequently digested to single cell suspension using Multi Tissue Mouse Tumor Dissociation Kit 1 (Miltenyi Biotec) and a gentleMACS OctoDissociator (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue of single mice was digested with trypsin and DNase according to the guidelines of the Neural Tissue Dissociation Kit (T) (Miltenyi Biotec) using a Gentle MACS Dissociator (Miltenyi Biotec) ...
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bioRxiv - Cancer Biology 2021Quote: ... Fresh tissue was cut into 1 mm pieces and dissociated at 37°C using the Human Tumor Dissociation Kit (Miltenyi Biotec) on a gentleMACS Octo Dissociator ...
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bioRxiv - Neuroscience 2022Quote: Membrane potential measurements were made by impaling pericytes on microvessels isolated from Cspg4-DsRed mice using a papain-based Neural Tissue Dissociation kit (Miltenyi Biotec), as described previously21,25 ...
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bioRxiv - Immunology 2022Quote: The tissue pieces were rapidly transferred into the gentleMACS C Tube containing Human Tumor Dissociation Kit (Miltenyi Biotec, #130-095-929) according to the manufacturer’s recommendation ...
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bioRxiv - Immunology 2019Quote: CD4+ T cells were enriched from PBMCs and tissue mononuclear cells using a negative selection magnetic-activated cell sorting (MACS) system as per manufacturer’s instructions (Miltenyi Biotec, Germany). Enriched CD4+ T-cells were stained with fluorescently conjugated antibodies listed in Table S2 ...
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bioRxiv - Cancer Biology 2021Quote: ... CD34+ human hematopoietic progenitor cells were isolated from human fetal liver tissue (obtained from Advanced Bioscience Resources) using the CD34 MicroBead Kit (Miltenyi Biotec) following the protocol provided by the manufacturer ...
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bioRxiv - Physiology 2021Quote: Muscle leukocytes were isolated from hamstring muscles four days post-surgery using a skeletal muscle dissociation kit (Miltenyi Biotech, cat#130-098-305) and GentleMACS Dissociator tissue homogenizer (Miltenyi Biotec) as previously described (19) ...
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bioRxiv - Developmental Biology 2021Quote: ... We subjected approximately 1g minced dissected villous tissue to the Miltenyi Tumor Dissociation Kit on the GentleMACS Octo Dissociator with Heaters (Miltenyi Biotec) to yield single-cell suspensions of viable placental cells in 5μM StemMACS™ Y27632 (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2021Quote: ... Primary neurons where derived from neural tissue following standard operational procedure from p0-1 mice brain using the Neural Tissue Dissociation Kit-Postnatal neurons (Cat. 130-094-802, Miltenyi Biotec). After removing the meninges ...
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bioRxiv - Developmental Biology 2020Quote: ... A region spanning from the subpallium to the DP (see Fig. 1A) was dissected on both hemispheres and dissociated using the Neural Tissue Dissociation Kit (P) (Miltenyi Biotec) and a gentleMACS Octo Dissociator with Heaters following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... and microglia was isolated with the Papain-based Neural Tissue Dissociation Kit (P) (# 130-092-628, Miltenyi Biotec B.V. & Co. KG) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: The soft palate from E13.5 embryos was dissected and digested using Multi Tissue Dissociate Kit 3 (Miltenyi Biotec, 130-110-204) at 37°C (600 rpm for 15 minutes ...
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bioRxiv - Immunology 2022Quote: ... brains were collected from P2-P5 pups and dissociated using the Neural Tissue Dissociation Kit (P) (Miltenyi Biotec, 130-092-628) on a gentleMACS Octo Dissociator (Miltenyi Biotec ...
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bioRxiv - Developmental Biology 2022Quote: ... a region spanning the dorsal pallial midline (see Figure 1A) was dissected on both hemispheres and dissociated using the Neural Tissue Dissociation Kit (P) (Miltenyi Biotec) on the gentleMACS Octo Dissociator following the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... Human tonsil tissue was processed by mincing with scissors followed by transfer of up to 4g of tissue to a gentleMACS C tube (Miltenyi Biotec) containing 8 mL of phosphate-buffered saline (PBS ...
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bioRxiv - Immunology 2022Quote: ... Further disaggregation of iCCA tissue into a single-cell solution for sequencing was completed using the MACS tumor dissociation kit (Miltenyi Biotec) with the standard tough tumor protocol ...
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bioRxiv - Neuroscience 2022Quote: Primary neurons were derived from the cerebral cortex of p0-p1 mice following standard operational procedure using the neural tissue dissociation kit-postnatal neurons (Cat. 130-094-802, Miltenyi Biotec) as previously described 30 ...
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bioRxiv - Neuroscience 2023Quote: Six organoids from each cell line were collected and processed using MACS papain neural tissue dissociation (Miltenyi Biotec #130-092-628) according to manufacturer’s protocol and resuspended in 0.22μm filter sterilized dPBS 0.04%BSA solution ...
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bioRxiv - Neuroscience 2023Quote: ... were sacrificed at E14.5 and brain cortices dissected under a stereomicroscope in ice-cold Hanks’ Balanced Salt Solution (HBSS) and enzymatically dissociated using a Neural Tissue Dissociation Kit (Miltenyi Biotec) following manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Tumours were collected into cold PBS then manually cut into small pieces and digested in GentleMACS C dissociation tubes using the GentleMACS tissue dissociator (Miltenyi Biotec) with the manufacture’s enzymes (liver dissociation kit ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 to 7 Nematostella polyps or 3 Aiptasia polyps constituted one biological sample and were transferred to tissue homogenization tubes (“C-tubes”, 130-096-334, Miltenyi Biotec) after they were relaxed ...
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bioRxiv - Cell Biology 2023Quote: ... from control and treated mice were collected and processed for isolation of stromal vascular cells (SVC) using the mouse adipose tissue dissociation kit (Miltenyi Biotec) and the gentleMACS™ Dissociator (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2023Quote: Mouse tumors were excised and ∼200Lmg of tumor tissue was enzymatically and mechanically digested using the mouse Tumor Dissociation Kit (Miltenyi Biotec) to obtain a single-cell suspension ...
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bioRxiv - Neuroscience 2023Quote: ... The mixture was then resuspended in Enzyme P using Neural Tissue Dissociation Kit P (Miltenyi Biotec, catalog no. 130-092-628) and was spun at 37°C for 15 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Tumor tissue was suspended in 35 uL of lysis buffer per mg of tissue and mechanically homogenized using M tubes (Milteny Biotec) with a gentleMACS Octo Dissociator (Miltenyi Biotec) using program “RNA_02” ...
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bioRxiv - Neuroscience 2023Quote: ... a single cell suspension from the hippocampus and cortex was prepared using Neural Tissue Dissociation Kit (papain-based) (Miltenyi Biotec GmbH). After blocking with CD16/CD32 antibody (clone 2.4G2 ...
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bioRxiv - Cell Biology 2023Quote: ... Freshly tumor (n = 8) and adjacent normal lung samples (n = 8) were delivered within MACS® Tissue Storage Solution (Miltenyi Biotec) on ice directly from the operating room to the laboratory after frozen pathology (1-2h).
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bioRxiv - Cell Biology 2023Quote: ... One ovary from each mouse was dissociated using a Multi Tissue Dissociation Kit 1 (cat# 130110201, Miltenyi Biotec, Bergisch Gladbach, Germany), following manufacturer’s instructions to create a single-cell suspension in D-PBS (cat# 14287080 ...
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bioRxiv - Molecular Biology 2023Quote: ... Aliquots of 500 µL of a single cell suspension of salivary tissue were incubated with 10 µL of FcR mouse blocking reagent for 10 minutes (Miltenyi Biotec), followed by 0.5 or 1 µL of each mAb for 30 minutes at room temperature ...
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bioRxiv - Neuroscience 2024Quote: We isolated single cells from the dissected mPFC sections using a neural tissue dissociation kit (Cat# 130-092-628, Miltenyi Biotec). After cell concentration quantified ...
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2P-NucTag: on-demand phototagging for molecular analysis of functionally identified cortical neuronsbioRxiv - Neuroscience 2024Quote: ... and the homogenate was pipetted up and down to further break apart tissue before being passed through a 30 μm cell strainer (Miltenyi Biotec) and collected in a 15 ml conical tube.
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bioRxiv - Cancer Biology 2024Quote: ... Tumors were detached from the mice and approximately 150 mg of tumor tissue was enzymatically and mechanically digested using the Mouse Tumor Dissociation Kit (Miltenyi Biotec) to obtain a single cell suspension ...
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bioRxiv - Bioengineering 2024Quote: ... The tissue was then physically dissociated by running the “Skeletal Muscle” program in the GentleMACS Dissociator (Miltenyi Biotec, #130-093-235). Next ...
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bioRxiv - Neuroscience 2020Quote: ... The brain tissues were cut into small pieces and dissociated into single cells using a modified version of the Neural Tissue Dissociation Kit (P) (130-092-628, Miltenyi Biotec, US). Myelin debris was removed using the Myelin Removal kit II (130-096-733 ...
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bioRxiv - Bioengineering 2021Quote: ... Nalm6 tumors were extracted on day 4 post bacteria treatment (day 2 post T cell treatment) and lymphocytes were isolated from tumor tissue by mechanical homogenization using a gentleMACS™ Dissociator (Miltenyi Biotec) in complete hTcm ...