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Citations for Miltenyi Biotec :
301 - 350 of 798 citations for PI 3065 CAS 955977 50 1 98% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... B cells were immunomagnetically separated using the MACS mouse B cells isolation kit (Miltenyi Biotec, Auburn, CA) and L.S ...
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bioRxiv - Cell Biology 2024Quote: ... The mitochondria pellet was resuspended in 100 μL storage buffer (130-094-532, Miltenyi Biotec, CA, USA) and stored at -80°C until further analyses ...
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bioRxiv - Immunology 2022Quote: ... The pellet was resuspended in 0.5 mL DPBS with 0.5 % BSA and labeled with 50 μL anti-CD14 microbeads (Miltenyi Biotec, catalog# 130-050-201). Cells were labeled for at least 20 minutes at 4 ° C and CD14+ cells were isolated by magnetic column (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... or from enriched mouse lung CD4+ T cells (CD4 T cell isolation kit, Miltenyi Biotec, Auburn, CA, USA), using RNeasy Micro Kit (Qiagen ...
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bioRxiv - Cancer Biology 2020Quote: ... After washing with cold MACS buffer stained cells were incubated with anti-biotin-microbeads (Miltenyi Biotec, Auburn, CA) for an additional 15 min and subsequently subjected to positive selection of Gr-1+ cells on a MACS LS column according to the manufacturer’s instructions (Miltenyi Biotec).
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bioRxiv - Cancer Biology 2020Quote: CD3+T-cells were isolated from healthy C57/B6 mice using a Miltenyi kit (Miltenyi Biotec, Auburn, CA). Round bottom plates were coated with 10μg of functional CD3 antibody (eBiosciences ...
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bioRxiv - Cancer Biology 2021Quote: Single cell suspensions from whole mouse lungs were prepared using the lung dissociation kit (Miltenyi Biotec, Auburn, CA) as per the manufacturer’s instructions ...
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Methacrylic acid-based biomaterials promote peripheral innervation in the subcutaneous space of micebioRxiv - Bioengineering 2022Quote: ... Tissue samples were homogenized using gentleMACS M tubes with the gentleMACS Dissociator (Miltenyi Biotec Inc., Auburn, CA, US). RNA isolation was completed using PuroSPIN™ Fibrous Tissue RNA Purification Kit (Luna Nanotech ...
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Methacrylic acid-based biomaterials promote peripheral innervation in the subcutaneous space of micebioRxiv - Bioengineering 2022Quote: Tissue explants (hydrogel with surrounding tissue) were placed in gentleMACS M tubes (Miltenyi Biotec Inc., Auburn, CA, US) containing lysis buffer on ice for 30 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... samples were incubated with CD11b antibody conjugated magnetic beads (Miltenyi Biotec, Catalog #: 130-093-634 San Diego, CA) for 15 min and then passed through a magnetic bead column (Quadro MACS Separator and LS columns ...
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bioRxiv - Immunology 2020Quote: ... covering the N-terminal S1 domain of the Spike protein of SARS-CoV-2 (Miltenyi Biotec, Auburn, CA) at 0.2 μg/mL and 0.5 μg/mL per peptide ...
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bioRxiv - Immunology 2021Quote: ... TCRβ+CD138- and TCR+CD138+ cells were further separated with anti-PE magnetic microbeads (Miltenyi Biotec, Auburn, CA). Depending on the experimental objective ...
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bioRxiv - Immunology 2020Quote: ... Neutrophils were isolated from BM cells by positive selection using the neutrophil isolation kit (Miltenyi Biotec, Auburn, CA). WT and G184S KI neutrophils were stained by Alexa Fluor 568- and Alexa Fluor 488-conjugated anti-mouse Gr-1 antibodies ...
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bioRxiv - Immunology 2022Quote: ... Total splenic B cells (CD19+) were purified using CD19 MicroBeads (130-121-301, Miltenyi Biotec, San Jose, CA), and CD19+CD43- non-B-1 cells were purified using Mouse B Cell Isolation Kit (130-090-862 ...
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bioRxiv - Immunology 2022Quote: Whole mouse lungs were digested and single cell suspension was prepared using dissociation kit (Miltenyi Biotec, Auburn, CA) as per the vendor’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Mitochondria were isolated using the MACS Mitochondria Isolation Kit (human tissue) (130-094-532, Miltenyi Biotec, CA, USA). Briefly ...
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bioRxiv - Immunology 2020Quote: ... A 1:1 mixture of CD3-depleted (Miltenyi Biotec) Tollip-/- (CD45.2+ ...
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bioRxiv - Cell Biology 2023Quote: ... insulin-like growth factor 1 (IGF-1, Miltenyi Biotec), FGF-2 and LDN193189 ...
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bioRxiv - Immunology 2022Quote: ... Alveolar-like MDMs were generated by differentiating CD4+ monocytes on tissue culture plates into macrophages in the presence of 50 ng/ml of human M-CSF (Miltenyi Biotec, Bergisch Gladbach, Germany) for 6 days ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then incubated in fresh media containing either 100 nM CCK or 100 nM saline vehicle control up to 72 hours with concurrent treatment with a mouse cytokine cocktail containing 50-ng/mL TNFα (Miltenyi Biotec, #139-101-687), 10-ng/mL IL-1β (Miltenyi Biotec ...
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bioRxiv - Bioengineering 2024Quote: ... frozen liver samples were added to ice-cold PBS at 50 mg/mL and processed for tissue homogenization using the gentleMACS Dissociator (n = 3 per group) (Miltenyi Biotec, Bergisch Gladbach, Germany). Then ...
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bioRxiv - Immunology 2023Quote: ... the cells were washed with PBS and resuspended in 50 µl of 1X annexin binding buffer containing 2 µl of FITC-annexin V (Miltenyi Biotec, 130-092-052). Cells were then incubated at room temperature for 30 min in the dark ...
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bioRxiv - Immunology 2022Quote: ... The pellet was resuspended in 50 ml EDTA-supplemented (2mM) washing medium and transferred over a 100 µm SmartStrainer (Miltenyi Biotec, Bergisch Gladbach, Germany). The cell suspension was then diluted to 90 ml ...
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bioRxiv - Immunology 2022Quote: ... Human monocytes were differentiated into a macrophage-like phenotype (MDM, monocyte derived macrophages) using M-CSF (50 ng/mL, Miltenyi Biotec, Bergisch Gladbach, Germany) for 6 days at 37ºC and 5% CO2 in Iscove’s Modified Dulbecco’s Medium (IMDM ...
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bioRxiv - Developmental Biology 2024Quote: Embryoid bodies (at least 50 EBs per sample) were pooled and dissociated using papain following the Neural Tissue Dissociation Kit – P (Miltenyi Biotec 130-092-628) and quenched with ovomucoid protease inhibitor (Worthington LK003182) ...
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bioRxiv - Immunology 2022Quote: ... Mouse CD4+ T cell isolation kit and CD8+ T cell isolation kit were obtained from Miltenyi Biotec (Auburn, CA). Clodronate and control liposomes were obtained from Liposoma (Amsterdam ...
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bioRxiv - Immunology 2020Quote: ... and TCRβ+CD138+ and TCRβ+CD138− cells were further separated with anti-PE magnetic MicroBeads (Miltenyi Biotec, Auburn, CA). After three washes with PBS ...
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bioRxiv - Neuroscience 2021Quote: ... sliced into 4 sagittal sections and placed into gentleMACS C-tubes (#130-093-237, Miltenyi Biotec, San Diego, CA), and processed for generation of single-cell suspensions using the Adult Brain Dissociation Kit and GentleMACS Octo Dissociator system (#130-107-677 and #130-095-937 ...
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bioRxiv - Immunology 2020Quote: ... Dermal cells were enriched for CD45-expressing cells using CD45 magnetic bead separation (Miltenyi Biotec,San Diego, CA, USA). Cell suspensions were then counted and/or labelled for flow cytometric phenotyping of surface expression markers or for flow sorting ...
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bioRxiv - Immunology 2021Quote: ... B cells were first isolated by magnetic cell sorting using the human CD19 MicroBeads (Miltenyi Biotec, San Diego, CA) and the AutoMACS magnetic cell separator ...
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bioRxiv - Immunology 2022Quote: ... Single cell suspensions from tumor tissues were prepared using the Mouse Tumor Dissociation Kit (Miltenyi Biotec, Auburn, CA, USA) and Percoll density gradient separation according to the manufacturer’s recommendations (GE Healthcare ...
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bioRxiv - Neuroscience 2023Quote: ... The cortices were dissociated using a neural tissue dissociation kit according to the manufacturer’s protocol (Miltenyi Biotec, Auburn, CA) and cultured in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Physiology 2023Quote: ... fresh mouse lung tissues were collected and dissociated using a lung dissociation kit (Miltenyi Biotec Inc., Auburn, CA, USA). Lung ECs were then isolated using two step magnetic bead separation (CD45 and CD31 antibody- conjugated microbeads ...
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bioRxiv - Genomics 2024Quote: ... 100–200 mg of the tumor tissue was placed in gentle MACS M tubes (Miltenyi Biotec San Diego, CA). 500 µL of QIAzol Lysis Reagent was added to the tissue ...
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bioRxiv - Immunology 2021Quote: ... CD3 (17A2; 1:400 or REA641; Miltenyi Biotec; 1:150); CD122 (TM-β1 ...
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bioRxiv - Genomics 2020Quote: ... poly-A RNA was isolated from 60∼90ug total RNA using μMACS mRNA Isolation Kits (Miltenyi Biotec Inc. CA, USA). Poly-A RNA (500 ng ...
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bioRxiv - Immunology 2020Quote: ... T cells were purified with either anti-CD8 or anti-CD4 magnetic beads using MACS columns (Miltenyi Biotec, Auburn, CA) prior to cell surface staining ...
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bioRxiv - Immunology 2021Quote: ... Lungs from infected mice were digested using the Miltenyi lung cell isolation buffer and dissociated via GentleMACS (Miltenyi Biotec, CA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... mice were euthanized and spleens were removed and mechanically disrupted with a GentleMacs tissue dissociator (Miltenyi Biotec Inc, Auburn, CA). Total spleen cells were washed with sterile PBS ...
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bioRxiv - Cell Biology 2022Quote: ... washed in Phosphate-Buffered Saline (PBS) and placed in C-tubes (gentleMACS C Tubes, Miltenyi Biotec Inc., San Diego, CA) and digested with collagenase and dispase as previously described.12,24 The resulting mixture was filtered through a 45 µm cell filter (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: Endothelial cells were enriched by depletion of CD45+ cells with CD45 microbeads (#130-052-301, Miltenyi Biotec, Auburn, CA, USA) followed by positive selection using CD31 microbeads (#130-097-418 ...
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bioRxiv - Immunology 2020Quote: ... CD14+ monocytes were isolated from peripheral blood mononuclear cells (PBMC) using magnetic bead selection (Miltenyi Biotec, San Diego, CA, USA). To differentiate cells into macrophages ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were enriched magnetically by their expression of H2Kk using a MACSelect Transfected Cell Selection kit (Miltenyi Biotec, Sunnyvale, CA). Cas9-mediated indel formation at the Acvr1 or Bmpr1a locus was confirmed by T7E1 mismatch analysis on the enriched cell populations (T7E1 Nuclease – New England Biolabs ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed twice using PBS containing 0.05% BSA at 1,700 RPM for 5 minutes and processed in a MACSQuant Analyzer 10 flow cytometer (Miltenyi Biotec, CA).
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bioRxiv - Cancer Biology 2023Quote: ... MNCs were enriched in CD34+ cells using magnetic separation with the CD34 Microbead Kit (Miltenyi Biotec, San Diego, CA, USA) and further purified by flow cytometric sorting as described below.
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bioRxiv - Immunology 2024Quote: ... or normal tissue were placed in 50 ml Falcon tubes containing 7-10 ml of MACS® Tissue Storage Solution (Miltenyi Biotec, cat. 130-100-008) and stored at room temperature for no longer than 2 hours ...
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bioRxiv - Immunology 2024Quote: ... total splenocytes and LN cells were resuspended in 500 μl of IMDM-10 and incubated with 50 μl of CD4 MicroBeads (Miltenyi Biotec, Cat. No. 130-117-043) at 4°C for 30 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... NM + 1 μM RA + 1 μΜ Purmorphamine (Miltenyi Biotec 130-104-465), was added and changed every other day until D17 ...
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bioRxiv - Biochemistry 2022Quote: Monocyte-and lymphocyte-enriched PBMC suspensions were sorted using Isolation kits and Depletion Column Type LS (Miltenyi Biotec Inc., Auburn, CA) according to the supplier’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were purified using a CD11b+ isolation kit per the manufacturer’s recommended protocol (Miltenyi Biotec, Auburn, CA; cat# 130-091-100). Prior to setting the culture ...