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Citations for Miltenyi Biotec :
301 - 350 of 801 citations for IL 13 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... incubated with human FcR- block (5µl/sample, Miltenyi Biotec) and stained with monoclonal antibodies (list of human antibodies is shown in Table S8) ...
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bioRxiv - Biophysics 2019Quote: ... 5% CO2 in the presence of IL-2 (50⍰ng/ml; Miltenyi Biotec, Bergisch Gladbach, Germany) and used 6 to 10⍰days after stimulation ...
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bioRxiv - Immunology 2021Quote: ... and IL-5 were enriched for live cells using a dead cell removal kit (Miltenyi Biotec) followed by CD138-APC (BD Pharmingen ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were subsequently stained with IL-17-FITC (1:20, rat, Miltenyi Biotec, 130-102-262), CD-3 APC/cy7 (1:200 ...
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bioRxiv - Biophysics 2019Quote: ... 5% CO2 in the presence of IL-2 (50 ng/ml; Miltenyi Biotec, Bergisch Gladbach, Germany) and used 7 days after activation ...
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bioRxiv - Immunology 2024Quote: ... supplemented with 1000 IU/ml GM-CSF and 400 IU/ml IL-4 (both Miltenyi Biotec) for 5 days ...
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bioRxiv - Molecular Biology 2023Quote: ... for monocyte-derived dendritic cells and 20 ng/ml IL-4 (#130-093-920, Miltenyi Biotec) and 40 ng/ml M-CSF (#130-096-485 ...
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bioRxiv - Neuroscience 2023Quote: ... At d15 cells were treated with IL-4 (20 ng/ml, LOT130-094-117, Miltenyi Biotec), IL-6 (100 ng/ml ...
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bioRxiv - Immunology 2023Quote: ... IL-4 (500 U/ml) and GM-CSF (800 U/ml, #130-093-868, Miltenyi Biotec) in a humidified ...
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bioRxiv - Microbiology 2023Quote: ... Peripheral blood mononuclear cells were obtained from deidentified healthy human donors (Gulf Coast Regional Blood Center, Houston, TX) and primary human monocytes isolated using MACS negative selection (Miltenyi Biotec, Cambridge, MA). E ...
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bioRxiv - Microbiology 2020Quote: ... Cells were washed twice with PBS 2% FCS before analysis by flow cytometry using a MACSQuant cytometer (Miltenyi Biotec, Germany) and analysis was done with the software FlowJo (vers 7.6).
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bioRxiv - Cell Biology 2021Quote: ... 0.5% heat-inactivated fetal calf serum (FCS) at pH 7.4 and magnetically sorted with using magnetic microbeads coated with antibodies directed against CD14 (Miltenyi Biotec). Monocytes were then seeded on plastic at 2×106 cells/well in six-well plates in RPMI 1640 (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... Whole blood staining was performed on 500µl blood samples from healthy donors with or without Fc fragment receptor blockers (Miltenyi Biotec). Whole blood staining was done with Alexa Fluor®647 labelled-VLP (2.5µg/ml ...
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bioRxiv - Biophysics 2021Quote: ... 0.5% heat-inactivated Fetal Calf Serum (FCS) at pH 7.4 and magnetically sorted with magnetic microbeads coated with antibodies directed against CD14 (Miltenyi Biotec). Monocytes were then seeded on glass coverslips at 1.5 × 106 cells/well in six-well plates in RPMI 1640 (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... in the presence of 50 ng/ml GM-CSF and 20 ng/ml IL-4 (Miltenyi Biotec). Untouched peripheral blood cDC1 and cDC2 (cDCs ...
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bioRxiv - Immunology 2019Quote: ... Cells were then washed and cultured in R10 medium containing IL-2 (100 IU/mL, Miltenyi Biotec). Culture supernatants were assayed on day 7 using an SIV p27 Antigen ELISA Kit (Zeptometrix) ...
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bioRxiv - Immunology 2020Quote: ... in combination with or without recombinant interleukin-4 (IL-4, Miltenyi Biotec, catalogue number: 130-094-061) for 24h ...
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bioRxiv - Immunology 2023Quote: ... moDCs were supplemented with a maturation cocktail: IL-6 (15 ng/ml, #130-093-933, Miltenyi Biotec), TNF-α (10 ng/mg ...
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bioRxiv - Genetics 2021Quote: ... CD14+ monocytes and CD19+ B cells were isolated from human PBMCs using the Human CD3+ T Cell Isolation kit (Miltenyi Biotec,130-050-101), Human CD14+ monocytes Isolation kit (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... FITC/PE anti-human REAfinity™ TCR Vδ1 (REA173) and APC anti-human REAfinity™ TCR Vδ2 (REA771) were purchased from Miltenyi Biotec (Bergisch Gladbach, Germany); PE/Cy7 anti-mouse FasL (MFL3) ...
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bioRxiv - Microbiology 2023Quote: ... CD14+ cells were isolated from the PBMCs using human CD14+ micro beads conjugated with anti-human CD14 mAbs (130-118-906, Miltenyi Biotec, Bergisch Gladbach, Germany) LC columns (130-042-401 ...
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bioRxiv - Cancer Biology 2022Quote: ... cryopreserved human PBMCs were thawed and primary human CD3+ T cells were isolated from PBMCs by using human Pan T Cell Isolation Kit (Miltenyi Biotec, 130-096-535). Cells were activated and expanded with Dynabeads at 1:1 ratio ...
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bioRxiv - Cell Biology 2020Quote: ... and Vioblue anti-human CD45 antibodies (REA747, #5180719178 Miltenyi Biotec) for 30 minutes at 4°C ...
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bioRxiv - Bioengineering 2022Quote: ... incubated with human and mouse FcR blocking reagents (Miltenyi Biotec) for 15min ...
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bioRxiv - Microbiology 2022Quote: ... PE-Vio770-conjugated anti-human CCR5 (clone REA245, Miltenyi Biotec), PE-conjugated anti-human CD11b (clone REA713 ...
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bioRxiv - Microbiology 2022Quote: ... and CD81 antibodies (Human Exosome Isolation Kit, Pan, Miltenyi Biotec) followed by EV isolation via magnetic separation and elution ...
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bioRxiv - Genetics 2022Quote: ... Then cells were incubated with human CD34 Microbeads (Miltenyi Biotec) and Fc receptor blocker in MACS buffer (1% BSA/2mM EDTA/PBS ...
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bioRxiv - Cancer Biology 2019Quote: ... we used CD45-VioBlue (human antibody, clone REA747, Miltenyi Biotec) to identify immune cells and in few cases ...
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bioRxiv - Genetics 2021Quote: ... Human CD14+ monocytes Isolation kit (Miltenyi Biotec, 130-050-201) and Human CD19+ B Cell Isolation kit (Miltenyi Biotec,130-050-301 ...
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bioRxiv - Molecular Biology 2021Quote: ... PBMCs were incubated with anti-human CD14 MicroBeads (Miltenyi Biotec) and monocytes were separated on a LS magnetic column on a QuadroMACS separator (Miltenyi Biotec) ...
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bioRxiv - Genomics 2021Quote: ... and APC anti-human CD99 (Miltenyi Biotec, #130-121-096). Cells were incubated for 15 min on ice and protected from light ...
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bioRxiv - Genomics 2021Quote: ... OctoMACS separator and CD19 MicroBeads (human) (all from Miltenyi Biotec) modifying slightly the data sheet protocol according to the experimental needs and the cell types (figure 1).
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bioRxiv - Immunology 2022Quote: ... Recombinant human transforming growth factor β1 (premium grade, Miltenyi Biotec) was diluted and used at the indicated concentrations.
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bioRxiv - Systems Biology 2023Quote: ... Monocytes were positively selected using Human CD14 MicroBeads (Miltenyi Biotec) and LS Columns ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-human GLAST (ACSA-1) biotinylated antibody (Miltenyi Biotec, Inc. ...
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bioRxiv - Molecular Biology 2024Quote: A MACSPlex human Exosome kit (Miltenyi Biotec, Bergisch-Gladbach, Germany) was used ...
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bioRxiv - Microbiology 2024Quote: ... in the presence of human FcR blocking reagent (Miltenyi Biotec) and subsequent staining with anti-PE paramagnetic microbeads (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... CD14+ monocytes were isolated using Human CD14 Microbeads (Miltenyi Biotec). Cell purity was verified by flow cytometry ...
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bioRxiv - Cancer Biology 2023Quote: ... Human T Cell TransAct (Miltenyi Biotec t# 130-111-160) was used to activate T cells ...
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bioRxiv - Neuroscience 2023Quote: ... and human recombinant TGF-β1 50 ng/mL (Miltenyi Biotec).
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bioRxiv - Cancer Biology 2023Quote: ... after treatment with a human FcR blocking reagent (Miltenyi Biotec). Flow cytometric analysis was performed as described above ...
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bioRxiv - Neuroscience 2024Quote: ... 20 ng/mL human stem cell factor (SCF, Miltenyi Biotec), 50 ng/mL vascular endothelial growth factor (hVEGF ...
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bioRxiv - Bioengineering 2024Quote: ... chambers were obtained from the Stanford Blood Center and primary human B cells were isolated by negative selection using the human B Cell Isolation Kit II (Miltenyi Biotec, cat: 130-091-151). Cells were cultured in Iscove’s modified Dulbecco’s medium (IMDM ...
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bioRxiv - Immunology 2021Quote: ... the cells were resuspended in PBS containing 2% FCS and 2mM EDTA and stained with the extracellular mix using APC-Vio770 anti-CD14 (clone REA599) from Miltenyi Biotec and used at 1/100 ...
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bioRxiv - Biochemistry 2021Quote: ... ACVR1-binding antibodies were enriched by incubation of biotinylated ACVR1-Fc and Myc-His monomeric ACVR1 at different concentrations with antibody expressing yeast cells followed by magnetic bead selection (Miltenyi Biotec) or flow cytometry on an Aria II cell sorter (BD Biosciences ...
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bioRxiv - Molecular Biology 2023Quote: ... and rinsed once with PBS and blocked in blocking buffer (PBS with 50% FCS, FcR Blocking Reagent 1:150 (Miltenyi Biotec) for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... After stopping the lysis with 1X PBS+10%FCS, cells were pelleted by centrifugation (300 x g, 5 min, 4°C) and subjected to dead cell removal (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... The cells were then strained through a 50 µm cell strainer and 1 million cells in 100 µL were incubated with 10 µL of Fc receptor block (Miltenyi Biotec) for 10 minutes on ice ...
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bioRxiv - Immunology 2023Quote: ... Add diluted 7-AAD (1:100 in the FACS buffer) to exclude dead cells and followed by Fc-receptor block (1:5 dilution, Miltenyi Biotec) to block non-specific staining ...
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bioRxiv - Bioengineering 2024Quote: ... rested overnight at a density of 1×107 cells/mL in 100 IU/mL IL-2 (Miltenyi Biotec)-supplemented medium ...