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Citations for Miltenyi Biotec :
301 - 350 of 951 citations for 5 1 3 Dioxolan 2 yl 2 3 fluorobenzoyl thiophene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... 0.4 x 106 isolated T cells from the same donor and 0.4 x 106 T cell activation beads (Miltenyi Biotec, anti-CD2/3/28) were added ...
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bioRxiv - Cell Biology 2024Quote: ... the pellet from each BM sample was resuspended in 392µl PBS/3% FBS and 8µl CD117 (cKit) MicroBeads (Miltenyi Biotec, catalog number 130-091-224) and incubated on ice for 30 mins ...
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bioRxiv - Immunology 2021Quote: Splenocytes or lung leukocytes were incubated with SARS-CoV-2 S peptide pools (1μg/ml, Miltenyi Biotec) for 24 h ...
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bioRxiv - Genomics 2021Quote: ... 2 µl of the respective antibodies were used: FITC anti-human CD10 (Miltenyi Biotec, #130-124-262) and APC anti-human CD99 (Miltenyi Biotec ...
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bioRxiv - Bioengineering 2020Quote: ... mouse anti-human ErbB-2 (CD340, HER2) Vio® Bright FITC (130-121-436) from Miltenyi Biotec; and rabbit anti-human HER2 mAb (2165S ...
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bioRxiv - Synthetic Biology 2024Quote: ... The cells were incubated with PBS supplemented with 2 μg/ml DAPI (Miltenyi Biotec, 130-111-570) for 15 minutes at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... with Fc receptors blocking prior to Anti-ACSA-2 MicroBeads (Cat# 130-097-678, Miltenyi Biotec, Germany). Incubation was carried out for 15 minutes in the refrigerator at 4℃ ...
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bioRxiv - Microbiology 2023Quote: Splenocytes or lung leukocytes were incubated with SARS-CoV-2 spike peptide pools (1μg/ml, Miltenyi Biotec) for 6 h in the presence of GolgiPlug (BD Bioscience) ...
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bioRxiv - Molecular Biology 2023Quote: ... Single-cell suspensions were prepared using Multi Tissue Dissociation Kit 2 (Miltenyi Biotec; Catalog # 130-110-203) following the instructions provided with the kit ...
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bioRxiv - Molecular Biology 2023Quote: ... The samples were then incubated with 2 mL of diluted RBC lysis buffer (Miltenyi Biotec, Auburn, CA) for 15 minutes at room temperature ...
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bioRxiv - Immunology 2024Quote: ... lymphoid organ cells were pulsed with a SARS-CoV-2 peptide pool (Miltenyi Biotec, # 130-127-309) (1 μg/mL ...
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bioRxiv - Immunology 2024Quote: ... CD8+ T cells were positively stained using CD8a (Ly-2) microbeads (Miltenyi Biotec, cat. # 130-117-044) and the negative flow-through fraction was collected ...
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bioRxiv - Cancer Biology 2024Quote: ... with a final concentration of 50 IU/mL of IL-2 (Miltenyi Biotec catalog #130-097-743). Cell-free supernatants were collected ...
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bioRxiv - Microbiology 2022Quote: ... after which 3 mL of FACS buffer was added and the mixture was passed over a magnetized LS column (Miltenyi Biotec, cat#130–042– 401). The column was washed once with 5 mL ice-cold FACS buffer and then removed from the magnetic field and 5 mL ice cold FACS buffer was pushed through the unmagnetized column twice using a plunger to elute the bound cell fraction.
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bioRxiv - Neuroscience 2022Quote: ... resuspended in the presence of anti-biotin MicroBeads (1:5; Miltenyi Biotec) and incubated for 15 min at 4°C ...
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bioRxiv - Immunology 2021Quote: ... The cell fraction was then depleted with human CD45 beads (100ul beads per 2×108 cells; Miltenyi Biotec) using autoMACS ...
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bioRxiv - Immunology 2020Quote: ... covering the N-terminal S1 domain of the Spike protein of SARS-CoV-2 (Miltenyi Biotec, Auburn, CA) at 0.2 μg/mL and 0.5 μg/mL per peptide ...
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Activation of innate immune cGAS-STING pathway contributes to Alzheimer’s pathogenesis in 5×FAD micebioRxiv - Neuroscience 2022Quote: Cell samples were fluorescently stained with Anti-ACSA-2-PE (clone: IH3-18A3) (130-102-365, Miltenyi Biotec), examined by a BD FACSCanto II flow cytometer ...
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bioRxiv - Bioengineering 2024Quote: ... and 2 mM EDTA and then isolated using mouse B cell purification kit (Miltenyi Biotec, 130-090-862) and LS columns (Miltenyi Biotec ...
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bioRxiv - Physiology 2022Quote: Primary cardiac fibroblasts were isolated from 2-day-old rats using a neonatal heart dissociation kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... lungs were harvested in 2 ml of Trizol and homogenized using a gentleMACS Octo dissociator (Miltenyi Biotec, Inc.). Total RNA was purified with trizol/chloroform ...
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bioRxiv - Neuroscience 2023Quote: ... Enzyme Mix 2 (15 μl of Buffer Y, 7.5 μl of Enzyme A [Miltenyi Biotec, 130-094-802]) was added for another 5-min ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 and 4 cytokines were added as followed: 20 ng/ml IL-4 (#130-093-920, Miltenyi Biotec) and 100 ng/ml GM-CSF (#130-093-864 ...
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bioRxiv - Immunology 2023Quote: ... and rested overnight with TexMACS medium supplemented with 100 IU/ml of recombinant human IL-2 (Miltenyi Biotec).
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bioRxiv - Cell Biology 2021Quote: Different lineages of in vitro cultured endMSCs (n = 3) were primed using a combination of recombinant pro-inflammatory cytokines and Toll-like receptors (TLR) ligands: IFNγ (Miltenyi Biotec Inc, San Diego, CA, USA) at 1 ng/ml and 100 ng/ml ...
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bioRxiv - Immunology 2021Quote: ... and PepTivator® SARS-CoV-2 Prot_S Complete peptide pool (Miltenyi Biotec, 15 mers with 11 amino acid overlap) covering the entire SARS-CoV-2 S protein ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then stimulated for 18 hours at 37 °C with SARS-CoV-2 S PepTivator peptides (Miltenyi Biotec) or media alone as a negative control ...
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bioRxiv - Cancer Biology 2022Quote: ... Spleens were placed in 3 ml of PBS supplemented with 2% FBS in C tubes and dissociated using program m_spleen_01 on a gentleMACS Octo dissociator with heaters (Miltenyi Biotec). Dissociated tissue was passaged through a 70 μm cell strainer and centrifuged at 1500 rpm x 5 minutes ...
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bioRxiv - Immunology 2022Quote: ... 500 IU/ml IL-2 and 10 ng/ml IL-15 (all the above regents were from Miltenyi Biotec). The above transfection mix was added into NK cell culture seeded in six-well plates (3×106/well ...
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bioRxiv - Genomics 2023Quote: ... pre-coated in 1 mM EDTA and deposited into a 5 mL ice-cold of PEB buffer (PBS, pH 7.2, 0.5% BSA, 2 mM EDTA; dilution of MACS BSA Stock Solution [Miltenyi Biotec, 130-091-376] 1:20 with autoMACS® Rinsing Solution [Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2024Quote: ... under continuous rotation at 150rpm and 37°C for 2 hours in MACS Gentle Dissociation C-Tubes (Miltenyi Biotec) to form a cell suspension ...
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bioRxiv - Immunology 2024Quote: CD4+ T cells were pre-enriched from spleens of CD45.1 or CD45.1/2 OT-II mice by negative selection using the CD4+ T-cell isolation kit (Miltenyi Biotec). Live (SytoxBlue−)CD25− CD44loCD62L+TCRβ+vα2+ naïve OT-II T cells were FACS-isolated ...
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bioRxiv - Neuroscience 2024Quote: ... then astrocytes were isolated using the anti-ACSA-2 magnetic cell sorting kit and protocol (catalog #130097678, Miltenyi Biotec). RNA was purified and concentrated with proteinase K cell digestion ...
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bioRxiv - Microbiology 2020Quote: ... Cells were washed twice with PBS 2% FCS before analysis by flow cytometry using a MACSQuant cytometer (Miltenyi Biotec, Germany) and analysis was done with the software FlowJo (vers 7.6).
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bioRxiv - Neuroscience 2021Quote: ... ACSA-2 labelled cells were flushed from the MS column with pre-warmed AstroMACS medium (Miltenyi Biotec, 130-117-031) supplemented 50 U/ml penicillin/streptomycin (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... Red blood cells and debris were removed and astrocytes magnetically labelled using ACSA-2 microbeads (Miltenyi Biotec, 130-097-678) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 femora and 2 humeri was harvested in PBS/2.5% FBS and pooled before lineage negative depletion using autoMACS Pro separator (Miltenyi Biotec). Cells were incubated with a lineage antibody cocktail (B220 ...
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bioRxiv - Genetics 2020Quote: ... were washed in PBS and MACS buffer (PBS, 2 µM EDTA, 0.5% BSA) and stained with Human CD34 Microbead kit (Miltenyi Biotec) following the manufacturer’s instruction for 30 minutes (4 °C ...
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bioRxiv - Immunology 2021Quote: ... the biopsies were cut into 2-3mm pieces and dissociated using a human Tumour Dissociation Kit (Miltenyi Biotec, Surrey, UK) according to the manufacturer’s protocol with some modifications ...
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bioRxiv - Neuroscience 2022Quote: ... then astrocytes were isolated using the Miltenyi Biotec’s anti-ACSA-2 magnetic cell sorting kit and protocol (Miltenyi Biotec, 130097678). RNA was purified and concentrated with proteinase K cell digestion ...
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bioRxiv - Bioengineering 2020Quote: ... The HER2 expression level was quantified by flow cytometry using anti-ErbB-2 (CD340) Vio® Bright FITC (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... we used NS medium containing media hormone mix (MHM) medium (KOHJIN-Bio) supplemented with 2% MACS NeuroBrew-21 (Miltenyi Biotec), 20 ng/ml basic FGF (PEPROTECH) ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were resuspended in MACS buffer (PBS containing 2 mM ETDA and 2.5% FBS) supplemented with human FcR Blocking Reagent (Miltenyi Biotec) at 1:5 dilution and anti-human CD45-APC (BioLegend 304012 ...
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bioRxiv - Immunology 2022Quote: ... CD8+ SP thymocytes were isolated using the CD8a+ (Ly-2) positive selection kit per manufacturer’s instructions (Microbeads, #130-117-044; Miltenyi Biotec). Flow-through resulted in the DN population ...
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bioRxiv - Pathology 2024Quote: ... The cells were resuspended in 200 μl of PBS containing 0.5% BSA and 2 mM EDTA and incubated with 15 μl of mouse FcR blocking reagent (Miltenyi Biotec) on ice for 15 min ...
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bioRxiv - Immunology 2024Quote: ... resuspended at 4 mio cells/ml and then cultured with HLA class I-enhanced neurons (see Human-iPSC-derived neuron preparation section above) at a density of 1.2mio cells/cm2 with IL-2 (1000UI/ml, Miltenyi Biotec) and a CD28 agonist antibody (5ug/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... Hearts were mashed with pestles for 2 min and cell isolation performed according to manufacturer’s instructions (Neonatal Heart Dissociation Kit, Miltenyi Biotec). Cell suspension was centrifuged for 5 min at 600 x g and cells were resuspended in cell culture medium and plated in 22.1 mm plates ...
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bioRxiv - Neuroscience 2023Quote: Samples and relevant controls were added with 2 μL of crystallizable fragment receptor blocker (Miltenyi Biotec, cat # 130-092-575) and incubated in the dark with rotation (12min ...
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bioRxiv - Developmental Biology 2023Quote: ... Astrocytes were isolated from the remaining cell suspension using the anti-ACSA-2 microbead kit (130-097-678, Miltenyi Biotec). The cell suspension was incubated with FcR blocking reagents followed by anti-ACSA-2 microbeads and placed on a magnetic MACS separator for positive selection ...
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bioRxiv - Bioengineering 2023Quote: ... Germany]) and activated using Dynabeads™ Human T-Activator CD3/CD28 Beads and 50 U/mL IL-2 (Miltenyi Biotec).