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Citations for Miltenyi Biotec :
301 - 350 of 906 citations for 2 1 1 Diethoxy 2 methyl propyl 4' Nitrophenyl Carbonate d6 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Astrocytes and microglia in the cell suspension were removed by using anti-ACSA-2 micro beads (130-097-679, Miltenyi Biotec) and anti-CD11b micro beads (130-049-601 ...
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bioRxiv - Immunology 2021Quote: ... were co-transferred with CD3+ cell-depleted autologous PBMCs (2 × 106) isolated using CD3 Microbeads and the LD Column (Miltenyi Biotec) into 5- to 7-week-old female mice ...
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bioRxiv - Microbiology 2021Quote: ... at a ratio of one bead per cell for 72 h and maintained in RPMI with 100 U/mL IL-2 (AIDS Reagents Program) and 100 ng/mL IL-7 (Miltenyi Biotec). Monocyte-derived-macrophages (MDMs ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated for 2 min at room temperature in the presence of red blood cell lysis buffer (Miltenyi Biotec S.I.) according to the manufacturer’s instructions ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... Lung cells were centrifuged and resuspended with fresh media with 20 μL/mL of PeptTivator® SARS-CoV-2 Prot_S (Miltenyi Biotec) and 5 μg/mL Brefeldin A (BioLegend) ...
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bioRxiv - Immunology 2020Quote: ... CD8+ T cells and CD4+ T cells were isolated from splenocyte suspensions using MACS MicroBeads (CD8a [Ly-2] and CD4 [L3T4] [Miltenyi Biotec]) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... CD8+ T cells and CD4+ T cells were isolated from splenocyte suspensions using MACS MicroBeads (CD8a [Ly-2] and CD4 [L3T4] [Miltenyi Biotec]) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... The organs were homogenized in 2 ml PBS using gentleMACS M tubes and gentleMACS Octo Dissociator (Miltenyi Biotec, Bergisch Gladbach, Germany). Afterward ...
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Single cell transcriptomics reveals the effect of PD-L1/TGF-β blockade on the tumor microenvironmentbioRxiv - Genomics 2020Quote: ... A red blood cell lysis step was performed by incubating cells for 2 minutes with 1x Red Blood Cell Lysis Solution (Miltenyi Biotec). Cells were washed again and then cell viability was measured using the Nexcelom Cellometer K2 Fluorescent Viability Cell Counter and the ViaStain AOPI Staining Solution (Nexcelom) ...
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bioRxiv - Microbiology 2020Quote: ... Each section was weighed and then homogenized in 2 ml PBS in gentleMACS M tubes and a gentleMACS Octo Dissociator (Miltenyi Biotec) using the RNA_1 setting ...
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bioRxiv - Immunology 2020Quote: ... PBMCs from 48 healthy individuals (samples taken before the SARS-CoV-2 outbreak) were enriched for CD19+ cells with CD19-microbeads (Miltenyi Biotec). For each individual ...
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bioRxiv - Immunology 2023Quote: ... T cells were stimulated by the addition of 100 IU/mL IL-2 (Proleukin) and anti-CD3/CD28 stimulation using loaded MACSiBead particles (Miltenyi Biotec) in a proportion of 1:1 of beads to cell ...
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bioRxiv - Bioengineering 2023Quote: ... and 2/3 for CD4+ subsets (both positive selection for CD4+CD25+cells and negative selection for CD4+CD25- cells Miltenyi Biotec). A small portion of each cell fraction was analyzed by flow cytometry to confirm enrichment of the desired cell subtype ...
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bioRxiv - Immunology 2023Quote: ... and incubated at 37°C for total of 30 minutes (15 minutes, 2 times) with rotated using MACSmix Tube Rotator (Miltenyi Biotec). Between and after the two incubations ...
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bioRxiv - Immunology 2023Quote: ... splenocytes were resuspended in CTL test media (Immunospot) and incubated at 37 °C for 2 hours with either no stimulation or S1 peptide simulation (Miltenyi Biotec) at a final concentration of 2 µg/mL before adding GolgiPlug to all samples for 4 additional hours ...
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bioRxiv - Immunology 2023Quote: ... Sarcoma tissue was collected on ice and immediately cut into 2 mm3 segments and dissociated to a single-cell suspension using the Human Tumor Dissociation Kit and gentleMACS dissociator (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: B cells were purified from the spleens of mice (aged 2–7 months of age) through negative selection with anti-CD43 magnetic beads (Miltenyi Biotec) and subsequently cultured in RPMI 1640 media (Life Technologies ...
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bioRxiv - Bioengineering 2023Quote: Lung cells were centrifuged and resuspended with fresh complete RPMI media with 20 μL/mL of PeptTivator® SARS-CoV-2 Prot_S (Miltenyi Biotec) and 5 μg/mL Brefeldin A (Biolegend) ...
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bioRxiv - Synthetic Biology 2023Quote: ... supplemented with 10% Human AB serum (Gemini Bio Products) with the addition of 100 U/ml human IL-2 IS (Miltenyi Biotec), 1 ng/ml IL-15 (Miltenyi Biotec) ...
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bioRxiv - Immunology 2024Quote: ... In-vitro CD4 T cell survival assays were performed in complete RPMI medium supplemented with recombinant murine IL-2 (100 ng/ml, Miltenyi Biotec) or IL-7 (10 ng/ml ...
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bioRxiv - Immunology 2024Quote: ... Pieces of approximately 1 cm2 were placed in 10 mL PBS containing 2% FBS and homogenized for 10min at room temperature using a gentleMACS dissociator (Miltenyi Biotec). Homogenized tissue was filtered through a 70 μm cell strainer and pooled ...
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bioRxiv - Cell Biology 2024Quote: ... cells were stained with ITGA7-APC and ITGA5-PE (Supplementary Table 2) and sorted using a MACSQuant Tyto Cell Sorter (Miltenyi Biotec). Flow cytometry was performed regularly during SC proliferation using a MACSQuant10 Flow Analyzer (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2024Quote: ... Then the astrocytes or newborn neurons were isolated using Anti-ACSA-2 MicroBead Kit or Anti-PSA- NCAM MicroBead Kit (Miltenyi Biotec). Briefly to say ...
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bioRxiv - Immunology 2024Quote: ... Cells were resuspended in 900 µl/107 of isolation buffer containing 0.5% Bovine Serum Albumin (BSA) and 2 mM EDTA in PBS and 10 µl/1x107 of mouse anti- Ly6G MicroBeads (Miltenyi Biotec) and incubated at 4°C for 10 mins ...
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bioRxiv - Cancer Biology 2024Quote: CD34+ cells were enriched from BM MNCs of sex-matched SF3B1-mutated MDS patients (n = 3) and healthy donors (n = 2) using CD34 Microbeads and positive selection with autoMACS Pro Separator (Miltenyi Biotec). 4000 or 7500 cells were plated as duplicates in MethoCult (H4434 ...
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bioRxiv - Immunology 2024Quote: OT-1 CD8 T cells were isolated from Thy1.1xOT-1 mice spleen by negative selection (Miltenyi Biotec).
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bioRxiv - Synthetic Biology 2020Quote: ... Dynabead-EV samples were re-suspended into 98 μl DPBS and 2 μl of one of the following antibodies: IgG1-PE (#130-113-200, Miltenyi Biotec, Germany), CD9-PE ...
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bioRxiv - Bioengineering 2021Quote: ... Nalm6 tumors were extracted on day 4 post bacteria treatment (day 2 post T cell treatment) and lymphocytes were isolated from tumor tissue by mechanical homogenization using a gentleMACS™ Dissociator (Miltenyi Biotec) in complete hTcm ...
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bioRxiv - Microbiology 2022Quote: ... 1 ml of each sample was transferred to a 2 ml Eppendorf tube and the samples were assayed on a flow cytometer (MACSQuant® VYB, Miltenyi Biotec). Green fluorescent cells were detected using the blue laser (488 nm ...
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bioRxiv - Microbiology 2022Quote: ... and stimulated with either SARS-CoV-2 S-trimer (10-100 µg/mL) or SARS-CoV-2 PepTivator® Peptide S and NP protein Pools (10 µg/mL each, Miltenyi Biotec) for 72 h at 37°C ...
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bioRxiv - Developmental Biology 2022Quote: ... The remaining cells were pelleted and resuspended in 80 µl of MACS buffer (PBS pH 7.2, 0.5% BSA and 2 mM EDTA) and 20 µl anti-CD45 MicroBeads (Miltenyi Biotec #130-052-301) or 20 µl anti-F4/80 MicroBeads (Miltenyi Biotec #130-110-443 ...
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bioRxiv - Biochemistry 2021Quote: ... The cell pellet was resuspended in 80 μL of 2% BSA/PBS and incubated with 20 μL of anti-PE MicroBeads UltraPure (Miltenyi Biotec, USA.) for 15 min at 4°C ...
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bioRxiv - Immunology 2021Quote: ... 2 mM EDTA in PBS) before positive selection of CD14+ monocytes by incubation with magnetic CD14 microbeads (Miltenyi Biotec, 130-050-201) (15 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Tumors were cut in small pieces of approximately 2 mm3 then incubated with Human tumor dissociation kit (Miltenyi Biotec, #130-095-929) and placed at 37°C in agitation (500 rpm ...
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bioRxiv - Cell Biology 2023Quote: ... cut longitudinally into roughly 2-mm-long pieces and were isolated using the Liver Dissociation Kit (mouse) and GentleMACS Dissociator (Miltenyi Biotec, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... + 0.5x GlutaMax (Thermo 35050061) + 1x NEAA (Thermo 11140050) + 1x N-2 (Thermo 17502048) + 1x MACS NeuroBrew-21 (Miltenyi Biotec 130-093-566). On D3 ...
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bioRxiv - Cell Biology 2023Quote: ... tissues were cleansed in ice-cold RPMI1640 and dissociated using the Multi-tissue dissociation kit 2 (Catalog No.130-110-203, Miltenyi Biotec, USA) following the manufacturer instructions ...
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bioRxiv - Immunology 2024Quote: CD4+ and CD8+ T cells and CD14+ monocytes were isolated from healthy donor mononuclear cells (agreement ANG-2003-2) by positive magnetic sorting (Miltenyi Biotec, Germany). Macrophages were generated by culturing monocytes for 7 days with 50 ng/ml M-CSF or 50 ng/ml GM-CSF (ORF Genetics Kopavogur ...
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bioRxiv - Immunology 2024Quote: ... both CD45.2 DKO and WT polyclonal donor marrow was depleted of mature T cells by magnetic column-based negative selection (Miltenyi Biotec MACS) using an anti-mouse CD5 biotin-conjugated antibody (Clone 53-7.3 ...
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bioRxiv - Molecular Biology 2020Quote: ... FLAG-PE (Miltenyi Biotec, 1:500 dilution), rabbit anti-TCAB1 (Bethyl Laboratories ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-Glast (Miltenyi Biotec, 1:100), rat anti-CD133 (eBioscience ...
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bioRxiv - Developmental Biology 2022Quote: ... 1% BSA (Miltenyi Biotec, 130-091-376), 1 mM DTT (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... APC-CD95-Fas (Miltenyi Biotec, 1:100). DAPI was used to stain the dead cells (ThermoScientific ...
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bioRxiv - Neuroscience 2020Quote: ... APC-CD133 (13A4, Miltenyi Biotec, 1/75), APC-Cy7-CD45 (BD ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-GLAST (1: 300, Miltenyi Biotec), mouse anti-caveolin1 (1:50 ...
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bioRxiv - Synthetic Biology 2020Quote: ... 1 ng/ml IL-15 (Miltenyi Biotec), and 4mM L-Arginine (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: ... 1% BSA (Miltenyi Biotec #130-091-376) and 0.1% Tween-20 (Sigma Aldrich #P7949 ...
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bioRxiv - Molecular Biology 2023Quote: ... and mEFSK4-APC (Miltenyi Biotec; 1:40).
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 1% NK MACS (Miltenyi Biotec), 5% human AB serum (Sigma-Aldrich ...
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bioRxiv - Synthetic Biology 2023Quote: ... 1 ng/ml IL-15 (Miltenyi Biotec). After 48 hours ...