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Citations for Miltenyi Biotec :
3301 - 3350 of 3977 citations for Mouse T cell receptor alpha chain C region TCRA ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... human CD3+ positive selection kit (# 130-050-101), and murine CD3+ and CD8+ negative selection kits (# 130-095-130, # 130-104-075) were purchased from Miltenyi Biotec. SiR-Actin (# CY-SC001 ...
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bioRxiv - Cancer Biology 2024Quote: ... 3.5 µL 1 mg/mL Hoechst 33342 dye and 399 µL of permeabilization buffer Inside Perm (#130-090-477, Inside stain Kit, MACS Miltenyi Biotec).
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bioRxiv - Immunology 2022Quote: ... was drawn from peripheral veins of healthy volunteers and subjected to the MACSxpress Whole Blood Neutrophil Isolation Kit (#130-104-434, Miltenyi Biotec). The blood was incubated with labeling antibodies and placed in the magnetic separator ...
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bioRxiv - Neuroscience 2023Quote: ... were sacrificed at E14.5 and brain cortices dissected under a stereomicroscope in ice-cold Hanks’ Balanced Salt Solution (HBSS) and enzymatically dissociated using a Neural Tissue Dissociation Kit (Miltenyi Biotec) following manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... we separate the epidermal tissue from the dermis and then dissociated to single-cell suspensions by combining mechanical dissociation with enzymatic degradation of the extracellular adhesion proteins using Epidermis Dissociation Kit for Human (130-103-464, MACS Miltenyi Biotec). The dissociated cell suspension was strained with a 40μm filter (BD Falcon) ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were transferred to a C Tube containing the enzyme mix I (enzyme P and buffer Z from the Adult Brain Dissociation Kit #130-107-677, Miltenyi Biotec, mixed according to manufacturer’s instructions) ...
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bioRxiv - Immunology 2023Quote: Monocytes were isolated from the bone marrow of Apoe-/-LysMCre and Apoe-/-LysMCreCnr1flox/flox mice by magnetic separation monocyte Isolation Kit (130-100-629, Miltenyi Biotec). The purified monocytes were labelled with carboxyfluorescein diacetate succinimidyl ester (CFSE ...
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bioRxiv - Neuroscience 2023Quote: ... 100 µl of CD81- (for all samples) or CD63- (for MS and HC samples) isolation beads (Exosome Isolation Kit, human Miltenyi Biotec) were added to 2 ml of diluted sample ...
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bioRxiv - Neuroscience 2023Quote: ... ipsilateral perilesional cortex and hippocampus from Sham and CCI mice were rapidly microdissected and a single cell suspension was prepared from the combined tissues (pooled tissue from two mice) using enzymatic digestion (Neural Tissue Dissociation Kit; Miltenyi Biotec) in combination with a gentleMACS Dissociator ...
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bioRxiv - Neuroscience 2023Quote: ... The mixture was then resuspended in Enzyme P using Neural Tissue Dissociation Kit P (Miltenyi Biotec, catalog no. 130-092-628) and was spun at 37°C for 15 min ...
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bioRxiv - Cancer Biology 2023Quote: ... tumors were extracted in a laminar flow hood under sterile conditions and were immediately digested using the human tumor dissociation kit (130-095-929, Miltenyi Biotec) in combination with the GentleMACS Octo Dissociator (Miltenyi Biotec) ...
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bioRxiv - Bioengineering 2023Quote: ... Lung progenitor cultures at day 14 of differentiation were detached as described above and fixed and permeabilized using the transcription factor staining buffer kit (Miltenyi Biotec). After washing with flow buffer (PBS w/o containing 1.0% BSA) ...
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bioRxiv - Genetics 2023Quote: PBS-perfused brains were cut into small pieces and dissociated using the Adult Brain Dissociation Kit and gentleMACS Octo Dissociator with heaters (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Primary BM mononuclear cells isolated from MM patients or healthy donors were enriched in CD138+ PCs using magnetic sorting with the CD138 Microbead Kit (Miltenyi Biotec). Cells were plated in 48-well plates previously seeded with human BM-derived mesenchymal cells.
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bioRxiv - Cancer Biology 2023Quote: The omental tumors harvested from C57Bl/6 mice were minced and dissociated using the Human Tumor Dissociation Kit from Miltenyi Biotec, following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Tissue was sectioned into pieces and a portion of tumour or normal brain was dissociated using the MACS Brain Tumor Dissociation Kit (Miltenyi Biotec). Labelled engrafted GFP+ cells were isolated from dissociated cell population using a BD FACSAria Fusion flow cytometer machine at the McGill University Health Centre Research Institute platform ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells were obtained from the lymph nodes (LNs) and spleens of OT-1 mice using a negative selection kit (130-104-075, Miltenyi Biotec). For CTL differentiation ...
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bioRxiv - Cancer Biology 2024Quote: ... tumour fragments from the lymphatic melanoma and skin lesion to chest wall metastases were obtained respectively during the surgical procedure and dissociation was performed on the same day utilising the human tumour dissociation kit (Miltenyi Biotec) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: Different sample types were subjected to bead-based multiplex EV analysis by flow cytometry (MACSPlex EV IO Kit, human, Miltenyi Biotec). Unless indicated otherwise ...
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bioRxiv - Cancer Biology 2021Quote: ... Enrichment of CD34+ cells was performed by immunomagnetic bead selection using an CliniMACS instrument (Miltenyi Biotec). After enrichment ...
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bioRxiv - Cell Biology 2020Quote: ... Stained cells were also quantified by Flow-cytometry in a MACSQuant-VYB (Miltenyi Biotec, Surrey, UK). Data analysis was carried out by using the FlowJo software ...
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bioRxiv - Developmental Biology 2021Quote: ... Pooled E18.5 sutures were similarly processed and red blood cell lysis (Miltenyi Biotec, 130-094-183) was performed after pelleting filtered cells ...
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bioRxiv - Neuroscience 2020Quote: ... ~100 μg Fe / 1 × 106 cells (40 μl Miltenyi Biotec FeraTrack SPIO / 2 ml culture medium) was chosen according to cell viability and manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were then trypsinized and incubated with anti-CD146 antibody coated microbeads (Miltenyi Biotec Inc.) for 15 mins at 4°C to obtain the CD140b+CD146+ cells for subsequent experiments ...
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bioRxiv - Immunology 2022Quote: ... MAIT cell staining (1 ×106 PBMC) was performed using specific surface monoclonal antibodies (All Miltenyi Biotec) namely ...
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bioRxiv - Cell Biology 2019Quote: ... The labelled cells were loaded onto a MACS LS Column (Cat# 130-042-401, Miltenyi Biotec), which is placed in the magnetic field of a MACS Separator ...
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bioRxiv - Immunology 2019Quote: ... Cells were washed and filtered through a LS magnetic column on a QuadroMACS separator (Miltenyi Biotec). Cells were then used in transcriptomic analysis ...
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bioRxiv - Genomics 2019Quote: ... The cell suspension was then labeled for oligodendrocyte marker 1:50 anti-O4-PE (Miltenyi Biotec), endhothelial cell marker 1:50 anti-CD31-PE (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2021Quote: Cerebrum and cerebellum at P5 were collected for cell dissociation using Neural tissue Dissociation (Miltenyi Biotec) kit with papain and double cell isolation using magnetic coupled antibodies anti-CD11B (Microglia ...
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bioRxiv - Neuroscience 2021Quote: Cerebrum and cerebellum at P5 were collected for cell dissociation using Neural Tissue Dissociation (Miltenyi Biotec). The cells were counted (Nucleocounter NC-200 ...
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bioRxiv - Neuroscience 2021Quote: ... cells were resuspended and incubated in MACS buffer containing Fc blocker and CD11c MicroBeads (Miltenyi Biotec) according to manufacturer instruction ...
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bioRxiv - Bioengineering 2021Quote: ... CD4+ and CD8+ cells were sorted by immunomagnetic separation using CD4 and CD8 microbeads (Miltenyi Biotec) in combination with a CliniMACS Prodigy cell sorter (Miltenyi Biotec) ...
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bioRxiv - Neuroscience 2020Quote: ... Supernatant was taken and filtered using a 30μm cell strainer (Miltenyi Biotec cat#130-041-407) into a collection tube ...
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bioRxiv - Neuroscience 2020Quote: ... The cell suspension was then incubated with anti-O4 micro beads (130-094-543, Miltenyi Biotec) for OPC purification.
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bioRxiv - Microbiology 2020Quote: ... or by magnetic-activated cell sorting (MACS) separation using CD14 microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany) as described previously 46 ...
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bioRxiv - Microbiology 2020Quote: ... Absolute cell numbers from each population were analyzed on a MACSQuant Analyzer 10 (Miltenyi Biotec Inc.) using FlowJo (AshLand) ...
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bioRxiv - Microbiology 2020Quote: ... B cells were enriched using immunomagnetic positive selection with anti-CD19 magnetic MACS beads (Miltenyi Biotec). Cells were washed twice with 5 ml FACS buffer with 3 mM EDTA and adjusted to a cell density of 1-2×106 cells/mL ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were subsequently stained with IL-17-FITC (1:20, rat, Miltenyi Biotec, 130-102-262), CD-3 APC/cy7 (1:200 ...
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bioRxiv - Neuroscience 2022Quote: ... the cells were labeled with human CD11b-APCVio770 and CD45-phycoerythrin (PE) (Miltenyi Biotec, Gladbach, Germany), and flow cytometry was performed as described previously (38) ...
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bioRxiv - Molecular Biology 2022Quote: ... The isolated Treg cells were expanded in culture for 14 days with TexMACS medium (Miltenyi Biotec) supplemented with 5% (v/v ...
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bioRxiv - Cancer Biology 2022Quote: ... A million immune cells were isolated using CD45 micro magnetic beads (Miltenyi Biotec, 130-052-301) from each tumor ...
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bioRxiv - Neuroscience 2021Quote: ... Myelin in the cell suspension was depleted by myelin removal beads (130-096-733, Miltenyi Biotec) and magnetic LD columns (130-042-901 ...
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bioRxiv - Immunology 2020Quote: ... B cells were purified with CD43 micro beads (130-049-801, Miltenyi Biotec, Bergisch Gladbach Germany), and 1×106 cells were placed in the top chamber of a transwell plate in 100μL of volume ...
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bioRxiv - Microbiology 2021Quote: ... and B-cells were affinity-purified using CD19 microbeads (MACS MicroBeads, Miltenyi Biotec, Bergisch Gladbach, Germany) resulting in >95% pure B-cell populations ...
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bioRxiv - Genetics 2022Quote: ... Dissociated cells were filtered through pre-separation filters (Miltenyi Biotec Inc. cat. no. # 130-095-823) and centrifuged at 150xg for 5 min ...
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bioRxiv - Immunology 2022Quote: ... B cells were first isolated from donors’ PBMC by MACS using human CD19 MicroBeads (Miltenyi Biotec). CD19+ B cells were then stained using LIVE/DEAD aqua fixable dead cell stain kit (Molecular Probes ...
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bioRxiv - Microbiology 2022Quote: ... Scraped cells were homogenized by two dissociation cycles on a gentleMACS Octo Dissociator (Miltenyi Biotec #130095937) using the RNA_02.01 program and 10 gentleMACS M tubes (Miltenyi Biotec #130093236) ...
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bioRxiv - Neuroscience 2022Quote: ... CD29-positive Müller cells were enriched using LS columns according to the manufacturer’s instructions (Miltenyi Biotec). Cells in the flow-through of the last sorting step – depleted of microglia ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were stained with the anti-CD326 (EpCAM) magnetic microbeads antibody (Miltenyi Biotec, 130-105-958) for 40 min on ice ...
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bioRxiv - Immunology 2019Quote: ... Total B cells were isolated from PBMCs using positive selection with MACS CD19 microbeads (Miltenyi Biotec). Next ...