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Citations for Miltenyi Biotec :
251 - 300 of 350 citations for LS Tetrasaccharide a LSTa Sialyl lacto N tetraose a >90% NMR Sodium salt since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: Liver cells were depleted of Ter119+CD45+ CD71+ CD117+ cells by magnetic cell separation using LS MACS Columns (Miltenyi Biotec). Cell suspensions were stained for 20-30 min at 4ºC in the dark with antibodies listed in Table 1 ...
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bioRxiv - Biochemistry 2021Quote: ... washed and passed through a LS-column according to manufacturer’s description (130-050-201, 130-042-401, Miltenyi Biotec, USA). The monocytes were counted (Sysmex) ...
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bioRxiv - Bioengineering 2021Quote: ... The enrichment or depletion was performed according to the manufacturer’s protocol and run over the LS or MS columns (Miltenyi Biotec, 130-042-401 or 130-042-201 ...
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bioRxiv - Bioengineering 2022Quote: ... cells were washed in a 10 ml of MACS buffer and loaded on LS columns (Miltenyi Biotec, 130-042-401) on the magnetic stand ...
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bioRxiv - Neuroscience 2024Quote: The LS MACS column was positioned in the magnetic field of a MACS Separator (Cat#130-090-976, Miltenyi Biotec) and rinsed with 3 mL of 0.5% BSA/PBS buffer ...
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Stromal transdifferentiation drives lymph node lipomatosis and induces extensive vascular remodelingbioRxiv - Pathology 2022Quote: ... CD45+ immune cells were depleted from the samples using CD45 beads and LS-columns following the manufacturer’s instructions (Miltenyi Biotec). The pellets were resuspended in Fc block (1:100 in FACS buffer containing EDTA and fetal bovine serum (FBS ...
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bioRxiv - Immunology 2024Quote: ... B cells were enriched by incubating cells with anti-CD43-biotin (S7) and magnetic enrichment with LS columns (Miltenyi Biotec). For class switching to IgG1 ...
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bioRxiv - Neuroscience 2024Quote: ... the pellet resuspended in 500 µl of D-PBS–0.5% BSA and then added to pre-wetted LS columns (Miltenyi Biotec). The columns were washed with 3 × 3 ml of D-PBS–0.5% BSA buffer after which the cells were flushed out using 5 ml of D-PBS–0.5% BSA buffer ...
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bioRxiv - Cell Biology 2024Quote: ... for 10 minutes at 4°C with rotation and passed through an LS separation column (Miltenyi Biotec, 130-042-401) inserted into a Quadro MACS Multi Stand magnetic stand (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2024Quote: ... Magnetic sorting of tumors was performed using the Miltenyi QuadroMACS Separator with LS columns (Miltenyi Biotec; cat. #130-042-401). CD11b (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2023Quote: ... Freshly tumor (n = 8) and adjacent normal lung samples (n = 8) were delivered within MACS® Tissue Storage Solution (Miltenyi Biotec) on ice directly from the operating room to the laboratory after frozen pathology (1-2h).
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bioRxiv - Cancer Biology 2024Quote: CD34+ cells were enriched from BM MNCs of sex-matched SF3B1-mutated MDS patients (n = 3) and healthy donors (n = 2) using CD34 Microbeads and positive selection with autoMACS Pro Separator (Miltenyi Biotec). 4000 or 7500 cells were plated as duplicates in MethoCult (H4434 ...
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bioRxiv - Immunology 2024Quote: ... DCs were purified (>90%) from the spleens of C57BL/6 mice using MACS CD11c microbeads (Miltenyi Biotec). Freshly isolated CD8+ T cells were then activated either with anti-CD3 and anti-CD28 antibody-coated microbeads at a 1:1 bead:cell ratio (Miltenyi Biotec ...
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bioRxiv - Immunology 2020Quote: CD4+ cells from the spleen of mice were purified using LS positive selection magnetic-activated columns (MACs) and anti-CD4 (L3T4) beads (Miltenyi Biotec). CD4+ MACs sorted cells were further purified by fluorescence activated cell sorting using a FACS Aria (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... treated with erythrocyte lysis buffer and CD45+ leukocytes were isolated using LS columns and CD45 MicroBeads (35 μL beads per sample, Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... We suspended microbead pellets in additional sorting buffer (500 μL for 108 cells) and then loaded the suspended microbeads to a LS column (Miltenyi Biotec). We washed the column that was attached to a magnetic MidiMACSTM separator from Miltenyi Biotec for retaining CD3 magnetic microbeads with the sorting buffer three times to remove CD3-negative cells and then transferred the beads bound with T cells to a 15 mL falcon tube ...
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bioRxiv - Biochemistry 2022Quote: Monocyte-and lymphocyte-enriched PBMC suspensions were sorted using Isolation kits and Depletion Column Type LS (Miltenyi Biotec Inc., Auburn, CA) according to the supplier’s instructions ...
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bioRxiv - Physiology 2022Quote: ... The cell pellet was re-suspended in a sorting buffer and then passed through a magnetized LS separation column (Miltenyi Biotec). The iron-containing superparamagnetic cells/aggregates were eluted from the demagnetized column ...
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bioRxiv - Immunology 2020Quote: ... lymphocytes were collected from spleens and lymph nodes of naïve CD45.1+ and CD45.1.2+ P14 TCR transgenic mice and negatively enriched for CD8+ T cells using the CD8a+ T Cell Isolation Kit and LS MACS Columns (Miltenyi Biotec). 1×106 CD8+ T cells were plated per well in 48 well flat-bottom plates ...
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bioRxiv - Immunology 2022Quote: ... Cells were counted and CD11b+ cells were selected by MACS column purification using the CD11b MicroBead kit and LS columns (Miltenyi Biotec). CD11b+ cells were stained with Fc block α-CD16/32 (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... after which 3 mL of FACS buffer was added and the mixture was passed over a magnetized LS column (Miltenyi Biotec). The column was washed once with 5 mL ice-cold FACS buffer and then removed from the magnetic field and 5 mL ice-cold FACS buffer was pushed through the unmagnetized column twice using a plunger to elute the bound cell fraction.
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bioRxiv - Developmental Biology 2023Quote: ... CD34+ cells were purified from a portion of the cells using the CD34 Microbead Kit Ultrapure and LS columns (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... single cell suspension prepared by physical and enzymatic dissociation followed by MACS sorting by LS columns (Miltenyi Biotec 130-042-401) with CD45+ cell removal using CD45 micobeads (Miltenyi Biotec 130-052-301 ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were resuspended in MACS staining buffer and passed through LS columns with a dead cell removal magnetic bead kit (Miltenyi Biotec). CD11b+-positive selection magnetic beads (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2022Quote: ... The pellet was resuspended in 6 mL MACS+ buffer and evenly distributed between two LS columns (Miltenyi Biotec 130-042-401) in the presence of a magnetic field (generated by the QuadroMACS magnetic separator ...
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bioRxiv - Neuroscience 2024Quote: ... the only exception being that the flow through from the MACS separation LS column was again loaded on the MACS separation MS column (Miltenyi Biotec) to ensure highest purity of the microglial fraction for TEM imaging ...
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bioRxiv - Neuroscience 2024Quote: ... The cell pellets were resuspended in 1 ml BSA/PBS and applied together with 1 ml BSA/PBS onto the prepared LS columns (# 130-042-401, Miltenyi Biotec) placed into a QuadroMACS™ Separator (# 130-091-051 ...
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bioRxiv - Immunology 2022Quote: ... Cells were counted and CD11b+ cells were selected by MACS column purification using the CD11b MicroBead kit and LS columns (Miltenyi Biotec). CD11b+ cells were Fc blocked with α-CD16/32 (Biolegend ...
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Mice generated with induced pluripotent stem cells derived from mucosal-associated invariant T cellsbioRxiv - Immunology 2023Quote: ... cells were stained with APC-labeled mMR1-tet and mMR1-tet positive cells were isolated with anti-APC microbeads and LS columns (Miltenyi Biotec). The isolated MAIT cells were intraperitoneally (IP ...
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bioRxiv - Cell Biology 2023Quote: ... Fibroblasts for RT-PCR and Western blot analyses were negatively sorted on Cd11b microbeads and positively sorted for anti-feeder microbeads through LS columns on a QuadroMACS magnet (Miltenyi Biotec). Fibroblasts for RNASeq ...
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bioRxiv - Immunology 2023Quote: ... Lineage depletion was carried out before cell sorting by MACS Cell Separation and LS columns (Miltenyi Biotec, cat#130-042-401) to deplete CD3+ ...
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bioRxiv - Cancer Biology 2023Quote: ... Dextran-loaded melanoma cells were cultured with LECs for 48 h after which the cells were sorted using MidiMacs separator and LS columns (Miltenyi Biotec). Due to the cessation of the production of these magnetic nanoparticles ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were washed twice and resuspended in 1mL of sorting buffer (up to 10⁸ cells) prior to magnetic separation on LS columns and the MACS Separator (Miltenyi Biotec). Columns were rinsed with 0.5mL of MACS buffer prior to loading the cell suspension ...
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bioRxiv - Immunology 2024Quote: ... CD45+ cells were enriched using anti-CD45 magnetic MicroBeads and LS columns (130-045-801 and 130-042-401, Miltenyi Biotec). For CD206-positive (CD206+ ...
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bioRxiv - Immunology 2024Quote: CD14-positive monocytes were isolated from peripheral blood mononuclear cells (PBMCs) with CD14 microbeads and MACS LS column system (Miltenyi Biotec). PBMCs were obtained from the buffy coats of twelve healthy blood donors (eight males and four females ...
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bioRxiv - Biochemistry 2024Quote: ... mouse cell depletion was performed on whole bone marrow cells using the Mouse Cell Depletion Kit and LS columns (Miltenyi Biotec). RIPA lysis was performed with purified human cells and 25µg of protein were loaded on 10% Bis-Tris-Gel NuPAGE gels with MOPS SDS running buffer (Life Technologies) ...
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bioRxiv - Immunology 2024Quote: ... donor OT1 cells that were present at low numbers post adoptive transfer were magnetically enriched with magnetic columns (LS Columns, Miltenyi Biotec). For early (≤ 72 h after immunization or infection ...
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bioRxiv - Immunology 2024Quote: ... the cells were resuspended in 1 mL of MACS buffer and applied to LS columns (Miltenyi Biotec, Cat #: 130-042-401) for enriching tetramer-bound T cells ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were washed once with FACS buffer and then loaded onto a pre-wet LS column (Miltenyi Biotec, #130-042-401) on the MACS Multistand (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... M and N protein were purchased from Miltenyi Biotec. Domains of SARS-CoV-2 S protein showed in Figure S2A was previously described (Wrapp et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... for 90 min at room temperature and purification was performed using the μMACS streptavidin starting kit (Miltenyi Biotec). Columns were first equilibrated with 1 mL of washing buffer (100 mM Tris-HCl at pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... from blood donors and monocyte populations (>90% CD14+) were isolated with CD14-negative selection magnetic beads (Miltenyi Biotec). Macrophages were obtained culturing these cells in the presence of 100 μg/mL of macrophage colony-stimulating factor (M-CSF ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were pelleted and washed in PBS with 0.5% BSA and 2mM EDTA and applied to MACS LS columns (Miltenyi Biotec, Auburn, CA) to gently separate microbead-bound podocytes from the other kidney cells ...
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bioRxiv - Neuroscience 2021Quote: ... the pellet was resuspended in MACS buffer and the solution was passed through LS columns (Miltenyi Biotec, Cat. No. 130-042-401). The cells were centrifuged at 300g for 15 min at 4°C ...
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bioRxiv - Immunology 2024Quote: ... The cells were further purified with magnetic beads and LS Columns from a Mouse neutrophil isolation kit (Miltenyi Biotec 130-097-658) resulting in 97-99% purity as determined by flow cytometry ...
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bioRxiv - Immunology 2023Quote: ... and following COVID-19 infection underwent CD19 and CD3 enrichment utilizing MicroBeads and LS Columns on the QuadroMACS™ Separator (Miltenyi Biotec). Single-cell suspensions of ∼1,200 cells/µl in PBS were prepared for scRNA-seq using 10X Chromium Next GEM Single Cell 5’ Kit v2 also using the Chromium Single Cell Human TCR and BCR Amplification Kits (10x Genomics ...
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bioRxiv - Microbiology 2023Quote: ... resuspended in 500 µl of staining buffer and applied onto a LS column for sorting of IgA+ and IgA- fractions with a QuadroMACS™ Separator (Miltenyi Biotec). IgA-coated and IgA-uncoated fractions were centrifuged and dry pellets were stored at -80°C until further analysis ...
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bioRxiv - Immunology 2022Quote: ... N and M peptide pool (Miltenyi Biotec, Bergisch Gladbach, Germany) for antigen specific T-cell stimulation ...
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bioRxiv - Cancer Biology 2020Quote: ... primary B cells from PBMCs using B Cell Isolation Kit II (Miltenyi Biotec, >90% purity by anti-CD19 staining). PBMCs were rested and stimulated with 100 ng/ml LPS (from E ...
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Single cell analysis of the cellular heterogeneity and interactions in the injured mouse spinal cordbioRxiv - Neuroscience 2020Quote: ... Pellet was resuspended in 1mL MACS buffer and applied onto equilibrated LS Miltenyi MACS Magnetic Bead Columns (Miltenyi Biotec Cat #: 130-042-401). Columns were washed with 2mL MACS buffer and suspension was centrifuged at 300g for 5min ...