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Citations for Miltenyi Biotec :
251 - 300 of 653 citations for Fc Receptor Like Protein 3 FCRL3 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... M and N protein pools [1 µg/mL] (PepTivator® SARS-CoV-2 regents; Miltenyi Biotec) in 96-well U-bottom plates at 0,5-1×106 PBMCs per well ...
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bioRxiv - Plant Biology 2022Quote: RNA immunoprecipitation (RIP) assays were performed with the μMACS GFP-Tagged Protein Isolation Kit (Miltenyi Biotec) according to manufacturer’s instructions with minor modifications ...
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bioRxiv - Cell Biology 2020Quote: Pre-conditioned media from FAP cell culture stimulated 3 days with PDGF-AA (20 ng/ml, human PDGF-AA, Cat nb. 130-108-983, Miltenyi Biotec) or control was collected and stored at −20°C ...
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bioRxiv - Immunology 2021Quote: ... T cells were activated with T cell activation/expansion CD2/3/28 beads at a ratio of 1:2 bead to cells (Miltenyi Biotec). Cultures were maintained in HPLM media with 5% dialyzed FBS (Sigma Aldrich ...
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bioRxiv - Immunology 2022Quote: ... supplemented with cytokines (IL-6 10ng/ml, IL-3 20ng/ml, M-CSF 50ng/ml and SCF 20ng/ml; all Miltenyi Biotec), with 2.5% Pen/Strep and 20μM L-Glutamine (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2020Quote: ... Cell clumps and debris were removed by two consecutive rounds of centrifugation 3 minutes at 200xg and filtration through 30 µm cell strainers (Miltenyi Biotec). Additional mechanical dissociation and removal of cell aggregates was achieved by gentle pipetting up and down 5 times using a P200 pipette and Gel Saver tips (QSP) ...
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bioRxiv - Immunology 2020Quote: ... after which 3 mL of FACS buffer was added and the mixture was passed over a magnetized LS column (Miltenyi Biotec). The column was washed once with 5 mL ice-cold FACS buffer and then removed from the magnetic field and 5 mL ice-cold FACS buffer was pushed through the unmagnetized column twice using a plunger to elute the bound cell fraction.
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bioRxiv - Immunology 2021Quote: ... 21 and 63 DPI samples (n=3) were depleted of alveolar macrophages using a MACS cell separation LS column (Miltenyi Biotec) after staining for CD14 and CD172a (see Table S2) ...
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bioRxiv - Developmental Biology 2022Quote: The soft palate from E13.5 embryos was dissected and digested using Multi Tissue Dissociate Kit 3 (Miltenyi Biotec, 130-110-204) at 37°C (600 rpm for 15 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... Remaining clumps of cells and debris were removed via two rounds of centrifugation for 3 min at 200 g followed by filtration through 30 μm cell strainers (Miltenyi Biotec). To ensure all remaining cells aggregates were dissociated ...
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bioRxiv - Biophysics 2022Quote: ... Naive T cells were purified via magnetic negative selection from spleens of 6–8 week old mice using either CD4+ (for 3.L2) or CD8+ (for P14 and OT1) T-cell isolation kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... JAM-1 and CD45 (Fig. 3, Supplementary Table 4) for 15 min prior to analysis on a MACSQuant10 Flow Analyzer (Miltenyi Biotec). To determine proportions of SCs and FAPs (Fig ...
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bioRxiv - Molecular Biology 2024Quote: ... program and cultured in TexMACS supplemented with 3% human serum (HS) (Life Science Production) and interleukin-2 (IL-2) (Miltenyi Biotec) for 24 hours ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 to 7 Nematostella polyps or 3 Aiptasia polyps constituted one biological sample and were transferred to tissue homogenization tubes (“C-tubes”, 130-096-334, Miltenyi Biotec) after they were relaxed ...
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bioRxiv - Bioengineering 2023Quote: ... and 2/3 for CD4+ subsets (both positive selection for CD4+CD25+cells and negative selection for CD4+CD25- cells Miltenyi Biotec). A small portion of each cell fraction was analyzed by flow cytometry to confirm enrichment of the desired cell subtype ...
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bioRxiv - Cell Biology 2024Quote: ... hFlt3-Ligand 100ng/mL, 130-096-479; hIL-3 60ng/mL, 130-095-069; TPO 10nM, 130-094-013, all from Miltenyi Biotec) and 1% PS during 24 hours ...
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bioRxiv - Cancer Biology 2024Quote: CD34+ cells were enriched from BM MNCs of sex-matched SF3B1-mutated MDS patients (n = 3) and healthy donors (n = 2) using CD34 Microbeads and positive selection with autoMACS Pro Separator (Miltenyi Biotec). 4000 or 7500 cells were plated as duplicates in MethoCult (H4434 ...
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bioRxiv - Molecular Biology 2022Quote: ... the same method was followed with the substitution of the fluorescent antibody mix against tetraspanins (CD63, CD81 and CD9) with the APC labelled HLA-G antibody (130111852, Miltenyi Biotec, same volume of 15 µl). This method ensured that EVs attached to the Ab conjugated beads were only detected if positive for HLA-G.
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated with magnetic bead-conjugated secondary antibody (Miltenyi Biotec). After further washes ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated with magnetic bead-conjugated secondary antibody (Miltenyi Biotec) for 15 minutes at 4°C ...
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bioRxiv - Immunology 2021Quote: ... anti-CD8α-APC-conjugated antibody and anti-APC microbeads (Miltenyi Biotec). Using the autoMACS® Pro separation system (Miltenyi Biotec) ...
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Reconstitution of prospermatogonial specification in vitro from human induced pluripotent stem cellsbioRxiv - Developmental Biology 2020Quote: ... and then was incubated with anti-SSEA1 antibody MicroBeads (Miltenyi Biotec) for 20 min on ice ...
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bioRxiv - Genetics 2020Quote: ... and CD28 antibodies (bead-to-cell ratio 1:2, Miltenyi Biotec). Resting or stimulated CD4 T cells were nucleofected with the MPRA plasmid library using at least 6 technical replicates for each sample ...
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bioRxiv - Genetics 2020Quote: ... and CD28 antibodies (bead-to-cell ratio 1:2, Miltenyi Biotec) and IL-2 (10ng/ml ...
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bioRxiv - Cell Biology 2021Quote: ... enriched with magnetic anti-CD15 antibodies (Miltenyi Biotec #130-046-601) and collected in HBSS+/+ buffer ...
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bioRxiv - Microbiology 2021Quote: ... Cell surface staining with anti-CD4 and CXCR4 antibodies (Miltenyi Biotec) confirmed than more than 95% cells were positive for both markers ...
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bioRxiv - Cell Biology 2022Quote: ... 10 μL of PAN T-Cell Biotin Antibody Cocktail (Miltenyi Biotec) was added and incubated for 5 minutes at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... CD133-PE (PROM1) antibody (Miltenyi Biotec, 130-113-670, 1:50), and CD338-PE (ABCG2 ...
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bioRxiv - Immunology 2021Quote: ... using the following antibodies: CD16 (APC) (#130-113-389, Miltenyi Biotec), CD14 (APC ...
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bioRxiv - Immunology 2020Quote: ... The APC-conjugated anti-CD154 antibody was obtained from Miltenyi Biotec. PE-conjugated anti-GR-1 (RB6-8C5) ...
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bioRxiv - Bioengineering 2022Quote: ... in DPBS) and 2 μL of CD34-PE antibody (Miltenyi Biotec). Cells were then incubated at 4°C for 10 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... microbeads containing antibodies directed against CD11b (130-093-634, Miltenyi Biotec) were used.
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bioRxiv - Bioengineering 2024Quote: ... anti-CD9 antibody conjugated with VioBlue was bought from Miltenyi Biotec Norden AB (Lund ...
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bioRxiv - Microbiology 2022Quote: ... and CD3 antibodies (T-cell activation/expansion kit from Miltenyi Biotec) were combined with isolated rhesus CD4 T cells as described by the manufacturer ...
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bioRxiv - Cancer Biology 2023Quote: Cells were stained with antibodies specific for PD-L1 (Miltenyi Biotec) and ErbB2 (R&D Systems ...
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bioRxiv - Immunology 2023Quote: ... and anti-CD138 antibodies in combination of streptavidin Microbeads (Miltenyi Biotec). CD19+ cells were then enriched using CD19 Microbeads (Miltenyi Biotec) ...
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bioRxiv - Immunology 2023Quote: ... biotin-conjugated anti-mouse CD55 REAfinity antibody (clone REA300, Miltenyi Biotec), unconjugated rabbit anti-mouse CD59a antibody (clone 108 ...
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bioRxiv - Bioengineering 2023Quote: ... CD31 antibody (anti-human, APC, REAfinityTM; 130-110-808; Miltenyi Biotec) [in 1:35 working concentration] and Hoechst 33342 [in 1:250 working concentration] were diluted in ECGM ...
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bioRxiv - Immunology 2024Quote: ... and anti-CD28 antibody coated beads (Miltenyi Biotec, Bergisch Gladbach, Germany) at a ratio of 3:1 (beads:cells) ...
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bioRxiv - Bioengineering 2024Quote: ... The anti-human antibody CD8β (REA715) was obtained from Miltenyi Biotec. The anti-mouse antibody mCD29 (HMβ1-1 ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were spun at 400 g for 3 minutes at 4°C and washed in MACS Buffer (Miltenyi Biotec, 130-091-222) supplemented with 4% FBS (FBS ...
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bioRxiv - Immunology 2021Quote: ... M and N protein pools separately [1 µg/mL each] (PepTivator® SARS-CoV-2 reagents; Miltenyi Biotec), Monensin (Biolegend ...
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bioRxiv - Plant Biology 2021Quote: ... Proteins fused to mCITRINE were immunoprecipitated using the pull-down kit Miltenyi μMacs anti-GFP (Miltenyi Biotec®). Crude extracts were put in 1XSDG supplemented with bromophenol blue and denaturated at 95°C for 5 min while IP samples were directly ready for migration on polyacrylamide gels.
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bioRxiv - Immunology 2020Quote: ... The splenocytes were incubated with the spike protein peptide pool at 1.5 μg/ml/peptide (Miltenyi Biotec, Germany) at 37 °C for 16-18 hours ...
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bioRxiv - Immunology 2020Quote: ... covering the N-terminal S1 domain of the Spike protein of SARS-CoV-2 (Miltenyi Biotec, Auburn, CA) at 0.2 μg/mL and 0.5 μg/mL per peptide ...
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bioRxiv - Immunology 2021Quote: ... CD8 T cells from spleens and lymph nodes of either P14 or FSF-Tim-3/E8i-Cre/P14 mice were purified by magnetic separation using a naïve CD8+ T cell isolation kit (Miltenyi Biotec #130-104-075) and then mixed with gp33-pulsed B cells at a ratio of 1:1 and incubated for indicated times at 37°C.
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bioRxiv - Molecular Biology 2023Quote: ... The protein complexes were then eluted into 1.5 mL Eppendorf tubes using 3 x 35 µL of pre-heated 95°C elution buffer (Miltenyi Biotec #130-042-701). The samples were heated to 95°C for 5 min ...
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bioRxiv - Developmental Biology 2024Quote: ... The cortex was cut in 2-3 mm pieces and dissociated in Neural Tissue Dissociation Kit P compounds (Miltenyi Biotec, 130-092-628) to obtain a cell suspension ...
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bioRxiv - Microbiology 2020Quote: ... antibody for 45 min at 4°C followed by the incubation with the secondary antibody anti-IgG1-PE (REA1017, Miltenyi Biotec, 1 μL/105 cells in 100 μL) for 30 min at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... with recommended dilutions of antibodies against CD31 (Miltenyi Biotec 130-097-418) and CD45 (Miltenyi Biotec 130-052-301 ...