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Citations for Miltenyi Biotec :
251 - 300 of 748 citations for 1 Benzyl 3 Tert Butyldimethylsilyl Oxy Methyl Piperidin 4 One since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Cytokines measured from PBMC supernatants were collected after 4 days of incubation with S protein and concentrations were predicted using a standard curve in the LEGENDplex assay (Miltenyi Biotec 741028).
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then stained with a CD34-PE-Vio770 antibody (20 minutes at 4°C; Miltenyi Biotec, clone AC136, #130-113-180) and DAPI (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... cells were resuspended in PBS with 0.5% of Bovine Serum Albumin (BSA, pH 7.2) and incubated for 15 min at 4 °C with myelin removal beads II (Miltenyi Biotec, Cologne, Germany) for myelin and debris removal ...
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bioRxiv - Microbiology 2023Quote: ... Neutrophils were isolated from 4 different donors using the MACSxpress Neutrophil Isolation and MACSxpress Erythrocyte Depletion kits (Miltenyi Biotec Inc., Auburn, CA) and suspended in RPMI 1640 (without phenol red ...
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bioRxiv - Neuroscience 2024Quote: Single cell suspensions of 1323-4 hiPSC-derived organoids were prepared with neural tissue dissociation kits (P) (Miltenyi Biotec, 130-092-628) following manufactural instructions ...
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bioRxiv - Immunology 2024Quote: ... the absence of exogenous Th17- promoting cytokines (ii) with 10ng/ml of human recombinant polarizing cytokines (i.e.,TGF-β, IL-4, IL-12; Miltenyi biotec, Germany) or WNT10a ...
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bioRxiv - Immunology 2024Quote: ... anti-CD14 and anti-BDCA-4 microbeads and subjected to AutoMACS selection (possel_s program followed by possel) (Miltenyi Biotec, Bergisch-Gladbach, Germany). Purity of isolated cells and ratios (PDC/B cells ...
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bioRxiv - Microbiology 2022Quote: ... after which 3 mL of FACS buffer was added and the mixture was passed over a magnetized LS column (Miltenyi Biotec, cat#130–042– 401). The column was washed once with 5 mL ice-cold FACS buffer and then removed from the magnetic field and 5 mL ice cold FACS buffer was pushed through the unmagnetized column twice using a plunger to elute the bound cell fraction.
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bioRxiv - Neuroscience 2022Quote: ... The remaining pellet was resuspended in 1.25 mL of Red Blood Cell Remover Solution and incubated for 10 minutes at 4 °C before the addition of 12 mL of 0.5 % bovine serum albumin (BSA, Miltenyi Biotec, # 130-091-376) diluted in DPBS ...
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bioRxiv - Immunology 2020Quote: ... and the remaining adherent monocytes were cultured for 48h in the presence of IL-4 (300 U/ml) and GM-CSF (450 U/ml) (both from Miltenyi Biotec, Madrid, Spain) in X-VIVO 15 (BioWhittaker ...
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bioRxiv - Cancer Biology 2022Quote: Samples were processed immediately after surgery and temporarily stored for transport at 4°C in tissue storage solution (Miltenyi Biotec, Bergisch Gladbach, Germany) as previously described ...
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bioRxiv - Developmental Biology 2024Quote: ... and Dlk1+ hepatoblasts were labeled with anti-Dlk mAb-FITC (MBL; D187-4; Rat) and anti-FITC MicroBeads (Miltenyi Biotec; 130–048-701). Labeled Dlk + hepatoblasts were isolated using a magnetic column (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2022Quote: ... for 15 minutes at 4°C prior to labelling using an anti-human CD95-APC antibody (clone DX2, Miltenyi Biotec, 130-117-701) or corresponding IgG1-APC isotype (clone IS5-21F5 ...
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bioRxiv - Physiology 2024Quote: ... Lysates were centrifuged (16,000 g, 10 minutes, 4°C) and the supernatants passed through 70 µm pre-separation filters (Miltenyi Biotec, Bergisch Gladbach, Germany). A fraction (10% ...
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bioRxiv - Neuroscience 2024Quote: ... Each cerebral hemisphere was then cut in 4 sagittal slides before being enzymatically and mechanically digested using a gentleMACSTM Dissociator (#130-093-235, Miltenyi Biotec, 37C_ABDK_01 program) and the Adult Brain Dissociation kit (#130-107-677 ...
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bioRxiv - Cell Biology 2021Quote: Different lineages of in vitro cultured endMSCs (n = 3) were primed using a combination of recombinant pro-inflammatory cytokines and Toll-like receptors (TLR) ligands: IFNγ (Miltenyi Biotec Inc, San Diego, CA, USA) at 1 ng/ml and 100 ng/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... APC antihuman CD271 (Clone ME20.4-1.H4, dilution 1/10, Miltenyi Biotec, Paris, France) and Brilliant Violet 510™ anti-human CD45 (clone HI30 ...
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bioRxiv - Neuroscience 2024Quote: ... 1% BSA (Miltenyi Biotec Ltd.), and 0.3% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... we prepared single-cell suspensions from spleens of 4- and 12-month-old APPtgp38fl/flLysM-Cre+/- and APPtgp38fl/flLysM-Cre-/- littermate mice with the Spleen Dissociation Kit (Miltenyi Biotec B.V. & Co. KG). After blocking with 25 μg/ml rat anti-mouse CD16/CD32 antibody (clone 2.4G2 ...
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bioRxiv - Microbiology 2020Quote: ... to analyze pDCs for 20 min at 4°C to analyze B cells or with two antibodies directed against CD303(BDCA2)-APC (Miltenyi Biotec, #130-097-931) and CD304(BDCA4)-PE (Miltenyi Biotec # 130-112-045) ...
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bioRxiv - Developmental Biology 2022Quote: ... The cells are centrifugated at 600 g for 7 min at 4 °C and resuspended with 180 μl running buffer (Miltenyi Biotec, 130-091-221) for MACS selection.
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bioRxiv - Plant Biology 2022Quote: ... Extracted protein samples were centrifuged at 20 000g for 2 min at 4°C and the supernatant was collected for immunoprecipitation using the µMACS GFP Isolation Kit (Miltenyi Biotec, MediRay, New Zealand). Total and immunoprecipitated proteins were resolved on a 4–12% SDS-PAGE gel ...
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bioRxiv - Immunology 2022Quote: ... After centrifugation (5 min, 500 x g, 4 °C) supernatant was discarded and cells were resuspended in MACS buffer (Miltenyi Biotec, #130-091-221) for following cell sorting.
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bioRxiv - Cancer Biology 2023Quote: ... For CD8+ T cells magnetic isolation we incubated the cell suspension 15 min incubation at 4°C with CD8 microbeads (Miltenyi Biotec, #130-045-201), then cells were passed through LS magnetic columns (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2023Quote: ... 2-4 mm pieces of tumors from huPDAC mice were treated with an enzyme mix from a tumor dissociation kit (Miltenyi Biotec, Bergisch Gladbach, Germany). The tissues were dissociated using a gentle MACS Dissociator (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2023Quote: Tumors from orthotopic PDAC mice were minced into 2-4 mm pieces and added with an enzyme mix from a tumor dissociation kit (Miltenyi Biotec, Bergisch Gladbach, Germany) in Roswell Park Memorial Institute medium 1640 (Cytiva ...
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bioRxiv - Cancer Biology 2023Quote: ... the sample is spun down at 400 g for 5 min at 4℃ and subject to the dead cell removal kit (Miltenyi Biotec, 130-090-101). Finally the sample is spun down at 400 g for 5 min at 4℃ and resuspended in 500 μL to 1 mL of PBS with 0.5% BSA to a final concentration of 700 to 1,500 cells per μl.
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bioRxiv - Developmental Biology 2024Quote: ... centrifuged (200 g for 3 min at 4°C) and resuspended in a solution composed by 80% FACS buffer and 20% anti-rat IgG MicroBeads (Miltenyi Biotec, 130-048-501). Cells were incubated in the mixture for 15 min at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Cells were labeled for at least 20 minutes at 4 ° C and CD14+ cells were isolated by magnetic column (Miltenyi Biotec, catalog# 130-042-201).
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bioRxiv - Cancer Biology 2020Quote: Dorsal skin was removed from euthanized 4-week-old C57BL/6 mice and dissociated with the Epidermis Dissociation Kit (Miltenyi Biotec, Cat. 130-095-928) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... TRA-1-60+ iPSCs were isolated from cultures using anti-TRA-1–60 MicroBeads (Miltenyi Biotec) and LS columns (Milteny Biotec) ...
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bioRxiv - Genomics 2020Quote: ... 1:100 B27 supplement (Miltenyi Biotec) and 1:40,000 Insulin (Sigma) ...
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bioRxiv - Immunology 2022Quote: ... GLAST (clone ACSA-1, Miltenyi Biotec). Cells were incubated with antibodies for 15 minutes ...
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bioRxiv - Immunology 2024Quote: ... and 1% Fc block (Miltenyi Biotec) in FACS buffer were added for 15 minutes (4°C) ...
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bioRxiv - Biochemistry 2024Quote: ... ODN 2216 (1 µM) (Miltenyi Biotec), or Poly:IC (1 µg/mL ...
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bioRxiv - Cancer Biology 2024Quote: ... CD107a and PD-1 (Miltenyi Biotec). For intracellular cytokine quantification ...
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bioRxiv - Immunology 2024Quote: OT-1 CD8 T cells were isolated from Thy1.1xOT-1 mice spleen by negative selection (Miltenyi Biotec).
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bioRxiv - Neuroscience 2020Quote: ... 10-20 cortices of PN day 2-4 pups were dissociated using the MACS Neural Tissue Dissociation Kit-T (Miltenyi Biotec, Cat# 130-093-231, Auburn, CA) at 37°C (5% CO2 ...
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bioRxiv - Immunology 2020Quote: ... Nylon wool-enriched splenic T cells from naive or immunized mice were incubated sequentially for 10 min at 4°C with FITC-conjugated anti-mouse γδ TCR or αβ TCR Abs and 15 min at 4°C with anti-FITC Microbeads (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany), then the cells were separated into bound and non-bound fractions on an autoMACS™ separator column (Miltenyi Biotec GmbH) ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was coupled with Anti-tomm22 micro-Beats (30min at 4° C) to remove free mitochondria through Magnetic-assisted cell sorting (MACS) (Miltenyi Biotec, 130-096-946, 130-042-401). The mitochondria-depleted flow-through was centrifuged at 13,000 × g for 10 min at 4° C to pellet synaptosomes (100ul of PBS resuspension ...
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bioRxiv - Molecular Biology 2020Quote: ... FLAG-PE (Miltenyi Biotec, 1:500 dilution), rabbit anti-TCAB1 (Bethyl Laboratories ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-Glast (Miltenyi Biotec, 1:100), rat anti-CD133 (eBioscience ...
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bioRxiv - Developmental Biology 2022Quote: ... 1% BSA (Miltenyi Biotec, 130-091-376), 1 mM DTT (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... APC-CD95-Fas (Miltenyi Biotec, 1:100). DAPI was used to stain the dead cells (ThermoScientific ...
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bioRxiv - Neuroscience 2020Quote: ... APC-CD133 (13A4, Miltenyi Biotec, 1/75), APC-Cy7-CD45 (BD ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-GLAST (1: 300, Miltenyi Biotec), mouse anti-caveolin1 (1:50 ...
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bioRxiv - Synthetic Biology 2020Quote: ... 1 ng/ml IL-15 (Miltenyi Biotec), and 4mM L-Arginine (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: ... 1% BSA (Miltenyi Biotec #130-091-376) and 0.1% Tween-20 (Sigma Aldrich #P7949 ...
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bioRxiv - Cell Biology 2022Quote: ... 1:50 (Miltenyi Biotec, #130-113-746). Cells were sorted using BD Accuri C6 flow cytometer and the data were analyzed using FlowJo (v10.6.1).
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bioRxiv - Molecular Biology 2023Quote: ... and mEFSK4-APC (Miltenyi Biotec; 1:40).