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Citations for Miltenyi Biotec :
2801 - 2850 of 3110 citations for Vascular Endothelial Cell Growth Factor VEGF Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... whole splenocytes from wild-type and Slc29a3−/− mice were sequentially incubated with biotinylated anti-mouse CD3 (clone 145-2C11)/CD19 (clone 6D5)/NK1.1 (clone PK136)/Ly6G (clone aA8)/TER-119/erythroid cells (clone Ter-119) and Streptavidin MicroBeads (Miltenyi Biotec, Germany). The magnetically labelled cells were removed using autoMACS (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: Treg and Tconv cells were enriched from PBMCs of patients with IBD and healthy donors using MACS CD4+CD25+ Regulatory T Cell Isolation Kit human (Miltenyi Biotec) for the co-culture experiments ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then labeled with Live/Dead dye and analyzed by MACSQuant Analyzer 10 Flow Cytometer (Miltenyi Biotec, San Diego, CA).
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bioRxiv - Biochemistry 2024Quote: ... Cells were washed three times with selection buffer and labeled with 10 μL streptavidin MACS microbeads (Miltenyi Biotec, 130-048-102) (sufficient for 1e7 cells) ...
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bioRxiv - Bioengineering 2024Quote: ... T cell isolation was performed on the CliniMACS system using anti-CD4 and CD8 magnetic beads (Miltenyi Biotec, San Jose, CA). Next ...
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bioRxiv - Genomics 2024Quote: All tumours were labelled with Annexin specific MACS beads using the Dead Cell Removal Kit (Miltenyi Biotec Cat# 130-090-101) following the manufacturers’ instructions and dead cells were removed by passing the labelled cells through the autoMACS® Pro (Miltenyi ...
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bioRxiv - Microbiology 2024Quote: ... Norway) centrifugation and used for CD4+ T cell isolation by negative selection with a commercial kit (Miltenyi Biotec, Bergisch Gladbach, Germany) per manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... the freshly collected whole lungs were dissected into single lobes to isolate single cells using a mouse lung dissociation kit (Miltenyi Biotec) and a gentleMACS™ Dissociator (Miltenyi Biotec) ...
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bioRxiv - Neuroscience 2024Quote: We isolated single cells from the dissected mPFC sections using a neural tissue dissociation kit (Cat# 130-092-628, Miltenyi Biotec). After cell concentration quantified ...
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2P-NucTag: on-demand phototagging for molecular analysis of functionally identified cortical neuronsbioRxiv - Neuroscience 2024Quote: ... and the homogenate was pipetted up and down to further break apart tissue before being passed through a 30 μm cell strainer (Miltenyi Biotec) and collected in a 15 ml conical tube.
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bioRxiv - Cell Biology 2024Quote: ... to serve as a control (i.e., unstimulated cells) or interleukin- 1β (IL-1β; 50ng/mL; Miltenyi Biotec, Cat#130-101-682) plus interferon-γ (IFNγ ...
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bioRxiv - Immunology 2024Quote: ... staining and single-cell sorting of PvAMA1-specific IgG+ MBCs were performed as follows: B cells were enriched using immunomagnetic positive selection with anti-CD19 magnetic MACS beads (Miltenyi Biotec) and stained with mouse anti-human CD20 (PE-Cy5.5 ...
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bioRxiv - Developmental Biology 2024Quote: ... 2mM EDTA) followed by addition of 10ml 1x red blood cell lysis (RBC) buffer (Miltenyi Biotec, Cat-Nr.: 130-094-183). Cells were incubated in RBC lysis buffer for 2 min at room temperature and then centrifuged at 600ɡ for 5 min at room temperature ...
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bioRxiv - Immunology 2024Quote: ... The supernatant was subjected to Magnetic Activated Cell Sorting (MACS) using CD11b magnetic microbeads (Order No. 130-093-634, Miltenyi Biotec) according to the manufacturer’s instructions and over LS columns after red blood cell lysis ...
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bioRxiv - Immunology 2024Quote: ... T cell stimulation was induced with anti-CD3ε and anti-CD28 beads (T Cell Activation/Expansion Kit mouse, Cat.#130-093-627, Miltenyi Biotec) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Aliquots of 500 µL of a single cell suspension of salivary tissue were incubated with 10 µL of FcR mouse blocking reagent for 10 minutes (Miltenyi Biotec), followed by 0.5 or 1 µL of each mAb for 30 minutes at room temperature ...
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bioRxiv - Genomics 2023Quote: ... Dissociated cells of each slice were collected with 6ml cold D-PBS and apply to a MACS SmartStrainer (70 μm, Miltenyi Biotec) placed on one well of a deepwell 24-well plate following centrifugation at 300×g for 10 minutes at 4°C ...
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bioRxiv - Immunology 2023Quote: Splenocytes or small intestinal IELs isolated from QFLTg or OT-1 mice were depleted of non-T cells using Pan T isolation Kit II (Miltenyi Biotec). The negatively selected T cells were then labeled with 5µM Cell Tracing Violet (CTV ...
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bioRxiv - Immunology 2024Quote: ... spleen or bone marrow cells were stained for 30 min on ice with Anti-CD23 Magnetic Microbeads (130-098-784, Miltenyi Biotec) or Anti-F4/80 Magnetic Microbeads (130-110-443 ...
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bioRxiv - Neuroscience 2023Quote: Six organoids from each cell line were collected and processed using MACS papain neural tissue dissociation (Miltenyi Biotec #130-092-628) according to manufacturer’s protocol and resuspended in 0.22μm filter sterilized dPBS 0.04%BSA solution ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were harvested and the transfection efficiency as well as expression of the NSD2 constructs in HEK293 cells were evaluated based on the mVenus reporter by flow cytometry (MACSQuant VYB, Miltenyi Biotec) and western blot using an anti GFP antibody (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: ... and 1:8 with CD8+ T cells enriched using mouse CD8 (Ly-2) Microbeads kit (Miltenyi Biotec Cat # 130-049-401) and labeled with CellTrace Violet (CTV ...
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bioRxiv - Immunology 2022Quote: ... to 1×108/ml and Ly6G+ cells were isolated according to the anti-Ly6G MicroBead Kit (#130-092-332, Miltenyi Biotec). The cells were labeled with biotinylated anti-Ly6G antibodies (anti-Ly6G-biotin ...
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bioRxiv - Molecular Biology 2023Quote: ... and stimulated with 200 ng/ml LPS (Enzo Bioscience) or anti-CD2/CD3/CD28-coated T-cell activation/expansion beads (Miltenyi Biotec) for 16h ...
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bioRxiv - Molecular Biology 2023Quote: PBMCs and CD14-depleted PBMCs were cultured in 96-well u-bottom plates at a density of 5 × 105 per well and stimulated with anti-CD2/CD3/CD28-coated T-cell activation/expansion beads (Miltenyi Biotec) in the presence of IL-2 (20 ng/ml ...
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bioRxiv - Immunology 2023Quote: ... 20’000 B cells from each condition were cultured for five days with 100’000 CTV-labelled CD4+ T cells along with CytoStim™ (200X dilution, Miltenyi Biotec). TransAct (300X dilution ...
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bioRxiv - Neuroscience 2023Quote: Analyses of the cells labeled in the previous section were conducted on a MACSQuant Analyzer 10 Flow Cytometer (#130-096-343, Miltenyi Biotec). Post-sort purity was assessed by the percent of CD11b+CD45+ singlets using MACSQuantify v2.13.0 software ...
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bioRxiv - Immunology 2023Quote: ... Cells from BRAFi (n = 5) or BRAFi/C-Me (n = 6) tumors were pooled and CD45+ cells were isolated using CD45 MicroBeads (mouse, Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Cd11b+ (130-126-725) and Ly6G+ (130-120-337) were isolated from single cell suspensions using UltraPure MicroBeads from Miltenyi Biotec according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... for all male cell lines (FD-1, FD-2, Ctrl) and the StemMACS mRNA reprogramming kit (Miltenyi Biotec, Bergisch Gladbach, Germany) for the female Fabry cell line (FD-3;26) ...
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bioRxiv - Immunology 2023Quote: ... purified CD8+ T-cells were coated with mouse IL-2 catch reagent from a mouse IL-2 secretion assay detection kit (Miltenyi Biotec) by incubating T cells in a 20x dilution of IL-2 catch reagent in R10 medium for 15 min on ice ...
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bioRxiv - Molecular Biology 2023Quote: ... single cell suspension prepared by physical and enzymatic dissociation followed by MACS sorting by LS columns (Miltenyi Biotec 130-042-401) with CD45+ cell removal using CD45 micobeads (Miltenyi Biotec 130-052-301 ...
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bioRxiv - Immunology 2023Quote: ... Tonsil tissue was roughly cut into smaller fragments using scissors and was further dissociated into a single cell suspension on the gentleMACS (130-096-334, Miltenyi Biotec) program “Multi_C_01_01” two times with a 15 minute incubation in between in a shaking water bath for 15 min-utes at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were resuspended in MACS staining buffer and passed through LS columns with a dead cell removal magnetic bead kit (Miltenyi Biotec). CD11b+-positive selection magnetic beads (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2023Quote: ... positive fraction cells are then run over a second magnetic column for higher purity) was used to elute highly pure CD11b+ cells in 500□μl of autoMACS Running buffer (#130-091-221, Miltenyi Biotec). Following separation ...
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bioRxiv - Molecular Biology 2023Quote: ... primary B cells were isolated from Rif1FH/FH and C57BL/6 (Rif1WT/WT) mice spleens using anti-CD43 MicroBeads (Miltenyi Biotec) and expanded in complete RPMI containing 5 µg/ml LPS (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: Single cell preparations of tumor-infiltrating lymphocytes were made by mechanically disrupting and enzymatically digesting tumors using gentleMACS™ (Miltenyi Biotec). The digestion media contained collagenase IV and dispase II (1 mg/ml each ...
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bioRxiv - Bioengineering 2023Quote: CD4+ and CD8+ T cells were then isolated from PBMCs by magnetic associated cell sorting (MACS) using the CD4+ or CD8+ human T cell isolation kit (130-096-533 or 130-096-495; Miltenyi Biotec), respectively ...
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bioRxiv - Immunology 2023Quote: ... and GC B cells were enriched on day 9 by depleting all CD43+ and all IgD+ cells with anti-mouse CD43 microbeads (Catalog:130-097-148, Miltenyi Biotec) and biotinylated anti-mouse IgD/anti-biotin microbeads (Catalog:130-090-485 ...
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bioRxiv - Immunology 2023Quote: ... Cells were magnetically labeled and separated according to the instructions of CD45 MicroBeads Human (order no. 130-045-801, Miltenyi Biotec). Then ...
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bioRxiv - Immunology 2023Quote: ... through peripheral blood mononuclear cell isolation by Ficoll density gradient centrifugation and magnetic separation using CD14 MicroBeads (130-050-201, Miltenyi Biotec), according to manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... or Kdm6aflox/flox LckCre (UTXTCD) 8- to 12-week-old female donor spleens using the CD4+ T cell Isolation Kit II (Miltenyi Biotec) according to manufacturer instructions ...
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bioRxiv - Immunology 2023Quote: SB CAR-T cells were generated as previously described from CD8+ or CD4+ bulk T cells purified from PBMCs of healthy donors using negative isolation with immunomagnetic beads (Miltenyi Biotec), activated with anti-CD3/CD28 beads according to the manufacturer’s instructions (Life Technologies ...
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bioRxiv - Immunology 2023Quote: LV CAR-T cells were generated as previously described from CD8+ or CD4+ bulk T cells purified from PBMCs of healthy donors using negative isolation with immunomagnetic beads (Miltenyi Biotec), activated with anti-CD3/CD28 beads according to the manufacturer’s instructions (Life Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... PDOX-derived cells were FACS-sorted (P8, nude control brain)23 or MACS-purified (remaining PDOXs) with a Mouse Cell Depletion kit (Miltenyi Biotec)21 ...
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bioRxiv - Pathology 2023Quote: ... The tissue was thereafter gently triturated with a needle and syringe and subsequently strained through a 70 µm cell strainer (130-098-458, Miltenyi Biotec).
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Mice generated with induced pluripotent stem cells derived from mucosal-associated invariant T cellsbioRxiv - Immunology 2023Quote: ... cells were stained with APC-labeled mMR1-tet and mMR1-tet positive cells were isolated with anti-APC microbeads and LS columns (Miltenyi Biotec). The isolated MAIT cells were intraperitoneally (IP ...
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bioRxiv - Immunology 2023Quote: ... The livers were harvested and dissociated into single-cell suspensions using the Liver Dissociation Kit on a GentleMACS Octo Dissociator (Miltenyi Biotec). The cell suspension was filtered through a 70 μm strainer ...
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A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: ... Cells were resuspended in 80 μL of buffer and 20 μL of Anti-biotin IgG microbeads up to 1×107 cells (Miltenyi Biotec) and incubated at room temperature for 15 minutes ...
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bioRxiv - Immunology 2023Quote: Tumor and bladder tissue were harvested and processed into single-cell suspensions using the Mouse Tumor Dissociation Kit and gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec) according to the manufacturer’s protocols ...