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Citations for Miltenyi Biotec :
201 - 250 of 1069 citations for Tumor Protein Translationally Controlled 1 TPT1 Antibody HRP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... tumors (0.5 g) were mechanically disrupted by chopping and then chemically digested using Tumor Dissociation Kit (130-096-730, Miltenyi Biotec) and gentleMACS Dissociator (130-096-427 ...
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bioRxiv - Cell Biology 2022Quote: ... Fresh tumor tissue from NSCLC patients were enzymatically digested and single-cell suspensions were stained with CD14 microbeads (Miltenyi Biotec) and positively selected using AutoMACS ProSeparator (Miltenyi Biotec) ...
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bioRxiv - Genomics 2020Quote: ... The sample was then macrodissected and dissociated into a cellular suspension by the gentleMACS Octo Dissociator using the human tumor dissociation kit as per manufacturer’s recommendations and the 37C_h_TDK_3 program (Miltenyi Biotec, Bergisch Gladbach, Germany). The suspension was used immediately for scRNA-seq ...
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bioRxiv - Genomics 2022Quote: ... The sample was then macrodissected and dissociated into a cellular suspension by the gentleMACS Octo Dissociator using the human tumor dissociation kit as per manufacturer’s recommendations and the 37C_h_TDK_3 program (Miltenyi Biotec, Bergisch Gladbach, Germany). The suspension was used for scRNA-seq ...
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bioRxiv - Cancer Biology 2023Quote: ... and further processed into dead-cell depleted single cell suspensions following manufacturer’s protocol using Tumor Dissociation Kit and MACS Dead Cell removal Kit (Miltenyi Biotec). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... were cut into small pieces and digested with in 2.4 mL of dissociation solution [DMEM medium supplemented with enzyme mixture (100ul Enzyme D, 50uL Enzyme R and 12.5uL Enzyme A)] using Tumor Dissociation Kit (Miltenyi Biotec). After incubation for 45 minutes at 37°C ...
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bioRxiv - Genomics 2024Quote: ... we processed a fraction of the dissociated tumor for immune cell depletion using CD11b MicroBeads (Miltenyi Biotec #130-097-142) following manufacturer’s protocol on LS Columns ...
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bioRxiv - Cancer Biology 2024Quote: ... tumors were harvested from mice on Day 3 and separately dissociated on a GentleMacs Octo Dissociator with Heaters (Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-human GLAST (ACSA-1) biotinylated antibody (Miltenyi Biotec, Inc. ...
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bioRxiv - Plant Biology 2023Quote: ... Horseradish peroxidase-conjugated anti-GFP antibody (1:5,000) (Miltenyi Biotec) was used to detect sYFP-tagged recombinant proteins ...
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bioRxiv - Cancer Biology 2021Quote: Freshly resected tumor or adjacent normal lung tissue was thoroughly chopped with surgical scissors and transferred to GentleMACS C Tubes (Miltenyi Biotec) containing 20 uL/mL Liberase TL (5 mg/ml ...
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bioRxiv - Immunology 2021Quote: Tumor nodules collected from the lungs of KP mice at time of analysis were cut into 1mm pieces and placed in mouse tumor dissociation kit (Miltenyi Biotec) for 45 minutes at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were minced with a scalpel and then enzymatically digested with a Tumor Dissociation Kit (Miltenyi Biotec, 130-095-929, Germany) on a gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: Each tumor was dissociated into a single cell suspension with Miltenyi’s gentleMACS Dissociator according to the tumor dissociation kit protocol (Miltenyi Biotec, Germany). A small portion of the dissociated tumor cells was removed for profiling via flow cytometry ...
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bioRxiv - Cancer Biology 2022Quote: ... The piece of cerebellum was dissociated into a single cell suspension by a gentleMACS Octo Dissociator within a gentleMACS C Tube in combination with the Brain Tumor Dissociation Kit (P) (Miltenyi Biotec) following all associated manufacturer’s protocols ...
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bioRxiv - Immunology 2022Quote: The tissue pieces were rapidly transferred into the gentleMACS C Tube containing Human Tumor Dissociation Kit (Miltenyi Biotec, #130-095-929) according to the manufacturer’s recommendation ...
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bioRxiv - Cancer Biology 2020Quote: CD8+ T cells were isolated from the spleens of transplanted PyMT tumor-bearing mice with a CD8a+ T Cell Isolation Kit (#130-104-075, Miltenyi Biotec), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... live CD45− cells were isolated by negative selection from digested WT and CCN4-KO tumors using the mouse CD45 (TIL) MicroBeads kit (Miltenyi Biotec). A total of 1 ×104 cells/well were cultured for 36 h in DMEM supplemented with 10% dialyzed FBS (Gibco ...
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bioRxiv - Cancer Biology 2021Quote: ... and from CD45− cells isolated from WT and CCN4-KO tumors by negative selection using a mouse CD45 MicroBeads kit (Miltenyi Biotec). Conditioned media were obtained by culturing 1 ×104 CD45− cells/well for 36 h in DMEM supplemented with 10% FBS ...
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bioRxiv - Immunology 2020Quote: ... Tumor and normal tissue samples harvested from euthanized mice were collected into ice-cold PBS and subsequently processed using a murine tumor digestion kit (Miltenyi Biotec) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... which were then dissociated with a tumor dissociation kit (Human) using a mechanical–chemical–mechanical cycle in a gentleMACS Dissociator (Miltenyi Biotec), according to the manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2021Quote: ... We subjected approximately 1g minced dissected villous tissue to the Miltenyi Tumor Dissociation Kit on the GentleMACS Octo Dissociator with Heaters (Miltenyi Biotec) to yield single-cell suspensions of viable placental cells in 5μM StemMACS™ Y27632 (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2020Quote: Single intestinal cells were obtained after enzymatic dissociation of the tunica muscularis using a Tumor Dissociation Kit (Miltenyi Biotec, Woking, UK), and YFP+ cells isolated using fluorescence activated cell sorting (FACS ...
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bioRxiv - Immunology 2022Quote: ... tumors from unimmunized or mice immunized with WTL+LPS+PGPC were harvested then dissociated using the tumor Dissociation Kit and the gentleMACS dissociator (Miltenyi Biotec), following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Further disaggregation of iCCA tissue into a single-cell solution for sequencing was completed using the MACS tumor dissociation kit (Miltenyi Biotec) with the standard tough tumor protocol ...
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bioRxiv - Molecular Biology 2022Quote: Dissociation of fresh Fallopian tube epithelium was done using the Milltenyi gentleMACS Octo Dissociator with heaters and the human Tumor Dissociation Kit (Miltenyi Biotec). Briefly ...
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bioRxiv - Immunology 2023Quote: ... Cells from BRAFi (n = 5) or BRAFi/C-Me (n = 6) tumors were pooled and CD45+ cells were isolated using CD45 MicroBeads (mouse, Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... tumors were extracted in a laminar flow hood under sterile conditions and were immediately digested using the human tumor dissociation kit (130-095-929, Miltenyi Biotec) in combination with the GentleMACS Octo Dissociator (Miltenyi Biotec) ...
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bioRxiv - Immunology 2023Quote: Tumor and bladder tissue were harvested and processed into single-cell suspensions using the Mouse Tumor Dissociation Kit and gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec) according to the manufacturer’s protocols ...
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bioRxiv - Immunology 2023Quote: ... and the appropriate programs were run according to the mouse tumor dissociation kit protocol and mouse lung dissociation kit protocol (both from Miltenyi Biotec) for tumors and lungs respectively ...
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bioRxiv - Immunology 2023Quote: ... Sarcoma tissue was collected on ice and immediately cut into 2 mm3 segments and dissociated to a single-cell suspension using the Human Tumor Dissociation Kit and gentleMACS dissociator (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Tissue was sectioned into pieces and a portion of tumour or normal brain was dissociated using the MACS Brain Tumor Dissociation Kit (Miltenyi Biotec). Labelled engrafted GFP+ cells were isolated from dissociated cell population using a BD FACSAria Fusion flow cytometer machine at the McGill University Health Centre Research Institute platform ...
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bioRxiv - Cancer Biology 2023Quote: Primary tumour epithelial cells were obtained using the gentle MACS Dissociator and Mouse Tumor dissociation kit (Miltenyi Biotec, Bisley, Surrey, UK) following the protocol recommended for ‘Dissociation of Tough Tumors’ ...
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bioRxiv - Cell Biology 2023Quote: ... Freshly tumor (n = 8) and adjacent normal lung samples (n = 8) were delivered within MACS® Tissue Storage Solution (Miltenyi Biotec) on ice directly from the operating room to the laboratory after frozen pathology (1-2h).
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bioRxiv - Molecular Biology 2024Quote: ... Each sample was dissociated for 15 min at 37 °C on the GentleMACS Octo Dissociator with Heaters (Miltenyi) using Human Tumor Dissociation Kit (Miltenyi Biotec). Following tissue dissociation ...
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bioRxiv - Immunology 2023Quote: ... New granulomas determined by PET-CT and uninvolved lung lobes (no granuloma present in the lobe) were enzymatically homogenized using a human tumor dissociation kit (Miltenyi Biotec) and a gentleMACS Dissociator (MiltenyiBiotec ...
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bioRxiv - Cancer Biology 2024Quote: ... and cryopreserved in FBS supplemented with 10% dimethyl sulfoxide (DMSO) at -80 °C (samples for genotyping and mass cytometry) or processed directly using Tumor Dissociation Kit (human, Miltenyi Biotec) as described below (fresh sample for mass cytometry) ...
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bioRxiv - Cancer Biology 2023Quote: CAFs were magnetically isolated from fully grown (Day15) melanomas of C57BL/6 mice using the mouse Tumor-Associated Fibroblast Isolation Kit as already described (Miltenyi Biotec). The samples were fixed in a 2.5% glutaraldehyde solution/ 0.1M Sodium Cacodylate Buffer (SCB ...
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bioRxiv - Biochemistry 2020Quote: ... and Anti-His-HRP (Miltenyi Biotec, Cat# 130-092-785).
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bioRxiv - Genetics 2020Quote: ... and CD28 antibodies (bead-to-cell ratio 1:2, Miltenyi Biotec). Resting or stimulated CD4 T cells were nucleofected with the MPRA plasmid library using at least 6 technical replicates for each sample ...
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bioRxiv - Genetics 2020Quote: ... and CD28 antibodies (bead-to-cell ratio 1:2, Miltenyi Biotec) and IL-2 (10ng/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... CD133-PE (PROM1) antibody (Miltenyi Biotec, 130-113-670, 1:50), and CD338-PE (ABCG2 ...
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bioRxiv - Cancer Biology 2019Quote: ... or isotype control antibody (mouse IgG1, Miltenyi Biotec, dilution 1:11) for 10 minutes in the dark at 2-8°C ...
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bioRxiv - Bioengineering 2021Quote: ... Nalm6 tumors were extracted on day 4 post bacteria treatment (day 2 post T cell treatment) and lymphocytes were isolated from tumor tissue by mechanical homogenization using a gentleMACS™ Dissociator (Miltenyi Biotec) in complete hTcm ...
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bioRxiv - Cancer Biology 2021Quote: ... Tumor tissue was minced by hand and then processed in a GentleMacs dissociator (130-093-235, Miltenyi Biotec GmbH, Bergisch Gladbach, Germany) according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... via dounce homogenization and 30% Percoll gradient or enzymatically digested using mouse tumor dissociation kit and gentleMACS™ Octo Dissociator (Miltenyi Biotec). Spleens and lymph nodes were either digested with Collagenase IV/DNAse or not digested before mechanical disruption ...
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bioRxiv - Cancer Biology 2024Quote: ... Tumors were dissected from the limb and dissociated by shaking for 45 min at 37°C in an enzyme mix from the tumor dissociation kit (Miltenyi Biotec, 130096730). Cell suspension was then strained through a 40 µm filter ...
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bioRxiv - Cancer Biology 2023Quote: ... melanomas of α-SMAcreAtg5fl/fl and Atg5fl/fl mice using the mouse Tumor-Associated Fibroblast Isolation Kit as already described (Miltenyi Biotec). RNA was extracted with the Macherey-Nagel NucleoSpin® RNA kit ...
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bioRxiv - Cancer Biology 2023Quote: CAFs were magnetically isolated from Day15 B16.F10 melanomas of C57BL/6 mice or α-SMAcreAtg5fl/fl mice using the mouse Tumor-Associated Fibroblast Isolation Kit as already described (Miltenyi Biotec). 5 x 104 cells CAFs were seeded on poly-L-Lysine coated glass coverslips ...
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bioRxiv - Neuroscience 2023Quote: Human tumor tissue samples were acquired during neurosurgical biopsy or open resection and stored in Tissue Storage Solution (Miltenyi Biotec, 130-100-008) for 2-30 hours until further processing for scRadiotracing ...